scholarly journals Rapid Quantification of Bacteria in Infected Root Canals Using Fluorescence Reagents and a Membrane Filter: A Pilot Study on Its Clinical Application to the Evaluation of the Outcomes of Endodontic Treatment

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Takuichi Sato ◽  
Keiko Yamaki ◽  
Naoko Ishida ◽  
Megumi Shoji ◽  
Emika Sato ◽  
...  
Keyword(s):  
Pilot Study ◽  
Root Canal ◽  
Membrane Filter ◽  
Bacterial Count ◽  
Root Canal Treatment ◽  
Polycarbonate Membrane ◽  
Root Canals ◽  
Infected Root ◽  
Present Pilot Study ◽  
Detection And Quantification

Objective. The bacterial examination has been performed during the course of the root canal treatment. In the present pilot study, the new developed method, using fluorescence reagents and a membrane filter, was applied to the detection and quantification of bacteria in infected root canals, in order to evaluate the outcomes of the treatment.Methods. Six infected root canals with periapical lesions from 5 subjects were included. Informed consent was obtained from all subjects (age ranges, 23–79 years). Samples from infected root canals were collected at the beginning of the treatment (termed #25 First), the end of the first day of treatment (termed #55 First), and the next appointment day (termed #55 Second). Then, the bacterial count (CFU) was measured using fluorescence reagents (4′,6′-diamidino-2-phenylindole and propidium iodide) and the polycarbonate membrane filter by Bioplorer.Results. The mean ± SD of CFU in the sample of “#25 First” was(1.0±1.4)×105. As the root canal treatment progressed, the CFU decreased as7.9×103(#55 First) and4.3×102(#55 Second).Conclusion. In the present pilot study, rapid detection and quantification of bacteria in infected root canals were found to be successfully performed using fluorescence reagents and a membrane filter (Bioplorer analysis).

scholarly journals Microbial Profile of Root Canals of Pulpally Infected Teeth In Ghanaians

2020 ◽  
Author(s):  
Akua Boakyewaa Konadu ◽  
Ebenezer Anno Nyako ◽  
Patrick Caldicock Ampofo ◽  
Moses Lorenzo Akyeh ◽  
Thomas Akuetteh Ndanu ◽  
...  
Keyword(s):  
Root Canal ◽  
Bacterial Species ◽  
Primary Root ◽  
Root Canal Treatment ◽  
Root Canals ◽  
Facultative Anaerobes ◽  
Microbial Profile ◽  
Laboratory Setup ◽  
Infected Root ◽  
Micro Organisms

Abstract Background Pulpal and periapical infections are initiated by microorganisms when they gain access into the dental pulp. The success of root canal treatment principally depends on the eradication of the micro-organisms in the root canal system. The aim of the study was to determine the microbial agents of infected root canals in Ghanaians patients. Methodology Forty four consecutive patients with sixty teeth referred to the Restorative Dentistry Clinic requiring root canal treatment were recruited. Root canal samples were collected from the teeth with sterile paper points. The samples were processed at the laboratory setup created at the chairside, subjected for microbial analysis and identification using Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS).ResultsA total of 259 isolates were recovered from the 60 infected root canals, belonging to twenty (20) different microbial genera. Out of the 259 microbial species isolated, only two (2) were candida albicans, a fungi; 257(99.2%) were bacterial isolates belonging to 19 genera. The 19 genera had 53 bacterial species, out of which 26(49.1%) were identified as facultative anaerobes, 15(28.3 %.) as obligate anaerobes and 12(22.6%) were aerobes. Streptococcus species (Streptococcus oralis, S.mitis, S. mutans and S. constellatus) were the most predominant isolates, followed by Prevotella sp, Actinomyces sp, Enterococcus faecalis and Rothia sp respectively. ConclusionThe findings of this study show that, primary root canal infections are polymicrobial with facultative anaerobes been predominant. The determination of the microbial profile aids in understanding the pathogenesis of pulpal and periradicular infections and help in choosing effective antimicrobial irrigation and medicament for root canal treatment.

Antimicrobial Effectiveness of Photodynamic Therapy, 5% Sodium Hypochlorite and 2% Chlorhexidine Gluconate in Root Canal Treated Teeth: A Clinical Study

2021 ◽  
pp. 232020682110189
Author(s):  
Ramesh Bharti ◽  
Aseem Prakash Tikku ◽  
Anil Chandra ◽  
Prashant Gupta
Keyword(s):  
Photodynamic Therapy ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Bacterial Species ◽  
Bacterial Count ◽  
Chlorhexidine Gluconate ◽  
Total Bacterial Count ◽  
Root Canals ◽  
Anterior Teeth ◽  
Infected Root

Objective: To evaluate the effectiveness of photodynamic therapy (PDT), sodium hypochlorite (NaOCl) and chlorhexidine gluconate (CHO) in the endodontic re-treatment in the removing of microorganisms from previously filled infected root canals. Materials and Methods: The study samples consist of 54 root canal treated anterior teeth with apical periodontitis were randomly divided into three groups according to the disinfection protocol. Microbiological samples from the root canals were collected after removing the gutta-percha (S1) and following the endodontic re-treatment (S2) with either 5% NaOCl, 2% CHO or PDT procedure for 1 minute. Microbiological samples were cultivated on selective plates to assess the presence or absence of microbial growth and determine the average reduction of viable microorganisms. Results: Significant reduction in the total bacterial count in all the groups at post treatment ( P < .001). PDT provided statistically higher (99.74%) elimination of Enterococcus faecalis in comparison to NaOCl (95%) and CHO (88%). Conclusions: The use of PDT in endodontic re-treatment led to the significant reduction of the remaining bacterial species. So, PDT can be used for routine endodontic disinfection in failed root canal cases.

Root Canal Treatment of A Maxillary Canine With Two Root Canals: a Case Report

2012 ◽  
Vol 2 (8) ◽  
pp. 406-407
Author(s):  
Dr. Ramta Bansal ◽  
◽  
Dr. Aditya Jain ◽  
Dr. Ramta Bansal
Keyword(s):  
Case Report ◽  
Root Canal ◽  
Root Canal Treatment ◽  
Maxillary Canine ◽  
Root Canals

scholarly journals Comparison of the Effects of a Bioceramic and Conventional Resin-Based Sealers on Postoperative Pain after Nonsurgical Root Canal Treatment: A Randomized Controlled Clinical Study

Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2661
Author(s):  
Kiche Shim ◽  
Young-Eun Jang ◽  
Yemi Kim
Keyword(s):  
Postoperative Pain ◽  
Postoperative Period ◽  
Root Canal ◽  
Continuous Wave ◽  
Root Canal Treatment ◽  
Root Canals ◽  
Anterior Teeth ◽  
Significant Difference ◽  
Ah Plus ◽  
Group 2

Background: This clinical trial aimed to compare the effects of bioceramic sealer and resin-based sealer on the incidence and intensity of postoperative pain. Methods: Patients with anterior teeth or premolars requiring root canal treatment were assigned to group 1 (n = 51). Those with molars requiring treatment were assigned to group 2 (n = 57). In groups 1En and 2En, root canals were obturated with Endoseal MTA using the single-cone technique. In groups 1AH and 2AH, the sealer used was AH Plus with the continuous wave technique. On the day of canal filling, each patient was instructed to indicate their pain intensity over the 7 day postoperative period, at rest and, while biting, using a visual analog scale. Results: There was no significant difference in the incidence or intensity of postoperative pain between the Endoseal MTA and AH Plus groups during the 7 day postoperative period (p > 0.05). Less time was needed to seal the root canals with Endoseal MTA, especially in group 2 (p < 0.05). Conclusions: Endoseal MTA and AH Plus had similar effects on the incidence and intensity of postoperative pain. The obturation time was shorter when using Endoseal MTA compared to AH Plus.

scholarly journals Management of Severe Curvatures in Root Canal Treatment with the New Generation of Rotating Files Using a Safe and Predictable Protocol

2020 ◽  
Vol 14 (1) ◽  
pp. 421-425
Author(s):  
Alfredo Iandolo ◽  
Alessandra Amato ◽  
Stefano Martina ◽  
Dina Abdel latif ◽  
Giuseppe Pantaleo
Keyword(s):  
Surface Area ◽  
Root Canal ◽  
Root Canal Treatment ◽  
Complex Root ◽  
Root Canals ◽  
New Generation ◽  
Area And Volume ◽  
Clinical Cases

Objective: The aim of this work was to show the management of severe curvatures done with the new generation of rotating files through a safe and predictable protocol. Methods: Three clinical cases were described. A safe protocol was explained using the latest generation of rotating files for the shaping of the anatomy of complex root canals. All the cases presented 90°, 45° curvatures and/or double curvatures. Results: The instrumentation of canals increased their surface area and volume. All the cases with 90° and 45° curvatures were optimally shaped. Conclusion: With the help of rotating files in the martensitic alloy, it is possible to work safely, while avoiding the risk of file separation, even in root canals with severe curvatures, thus achieving success even in complex clinical cases.

scholarly journals Treatment of anatomic canal variations in premolar teeth: Five case reports

2021 ◽  
Vol 11 (Suppl. 1) ◽  
pp. 279-284
Author(s):  
Emre Bodrumlu ◽  
Esma Dinger
Keyword(s):  
Root Canal ◽  
Native Speakers ◽  
Case Reports ◽  
Anatomical Variations ◽  
Root Canal Treatment ◽  
Root Canals ◽  
Treatment Indications ◽  
Native Speakers Of English ◽  
Root Canal Morphology ◽  
Root Canal Instrumentation

Aim: The aim of this case report is to present a series of anatomical variations and endodontic treatments in four two-canal mandibular premolar teeth and three rooted three-canal maxillary second premolar teeth with root canal treatment indications identified via clinical and radiographic examinations. The success of root canal treatment is achieved with a thoroughly examined root canal morphology that has been accurately determined radiographically and clinically before adequate shaping, irrigation, and hermetic filling procedures. Root canals that are not found or not adequately disinfected can cause root canal treatment failure and complications, such as pain, swelling, or persistent fistula, also known as flare-up, after treatment. Canal variations in the teeth were detected via periapical radiographs during the root canal instrumentation stage. Methodology: The endodontic treatments of four two-canal mandibular premolar teeth and one triple-rooted three-canal maxillary second premolar with root canal treatment indications were described. Conclusion: To achieve full success in root canal treatment, anatomical variations should be examined in detail before and during treatment, and treatment should be completed with appropriate techniques.   How to cite this article: Dinger E, Bodrumlu E. Treatment of anatomic canal variations in premolar teeth: Five case reports. Int Dent Res 2021;11(Suppl.1):279-84. https://doi.org/10.5577/intdentres.2021.vol11.suppl1.41   Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.  

scholarly journals Knowledge and Practices Assessment of Decontamination during Root Canal Treatment in Saudi Arabia

2021 ◽  
Vol 6 (01) ◽  
pp. 25-29
Author(s):  
Necdet Adanir ◽  
Hassan Alkhalaf ◽  
Maram H. Alanazi ◽  
Asma S. Alghamdi ◽  
Abdullah A. Aljami ◽  
...  
Keyword(s):  
Root Canal ◽  
Cross Sectional Study ◽  
Root Canal Treatment ◽  
Canal System ◽  
Cross Sectional ◽  
Rubber Dam ◽  
Infected Root ◽  
Treatment Data ◽  
Root Canal System ◽  
Partial Isolation

Introduction: Bacteria play a crucial role in the pathogenesis of pulpal and periradicular pathoses. For example: Apical periodontitis is an inflammation of dental periapical tissues developed as a response to colonization of microorganisms in root canal system. The elimination of microbial species from the infected root canal system necessitates a strict aseptic condition for Instrumentation, disinfection and inter appointment medication. Enterococcus faecalis and Candida albicans had been commonly associated organisms in treatment-resistant infections. Methods: This was an observational cross-sectional study based on web-survey questionnaires developed by the authors with high confidence. The sample size was 304 estimated using the Qualtrics calculator with a confidence level of 95% and a margin of error of 5%. The questionnaire is divided into sections, regarding endodontic practice include endodontic education , years of experience , used of rubber dam or no and how to used irrigant solution whoever Sodium hypochlorite (NaOCl), Chlorhexidine, chelating agents (EDTA) or (MTAD) and reasons of every irrigant that prevents during root canal treatment. Data were collected using Google forms and prepared for analysis using Microsoft Excel. Statistical analysis was performed using the SPSS software. Results: A total of 304 participants responded, 44.1% dental interns, 39.5% General Dentist, 9.9% Endodontist, 6.3% other dental specialty. the Probable reasons that prevent them using rubber dam during root canal treatment are: 5.9% due to difficulty to apply, 9.2% due to lack of materials, 5.3% because it is a time-consuming, 1.6% said that other isolation methods are enough (partial isolation), 3.9% because of multiple reason combined together. 48.7% they always use rubber dam, so they did not choose any of the previous reasons. Conclusion: In conclusion, we identify and understand, most of the participants “always using rubber dam” during root canal treatment and the most common reasons was time consumption that prevented dentist from using rubber. In this study majority of participants preferred NaOCl 81.9% and 7.1% don’t use it.

Retreatment and Restoration Using Individual Formable Fibre Post and Crown - Case Report

2020 ◽  
Vol 48 ◽  
pp. 15-21
Author(s):  
Rinda Wanodyatama ◽  
Tri Endra Untara ◽  
Tunjung Nugraheni
Keyword(s):  
Case Report ◽  
Root Canal ◽  
Root Canal Treatment ◽  
Root Canals ◽  
Gutta Percha ◽  
Surgical Methods ◽  
Dental Hospital ◽  
Conservative Dentistry ◽  
One Year ◽  
Endodontic Retreatment

Endodontic retreatment is an attempt to reachieve a healthy periapical after it was previously been carried out an inadequate or reinfected endodontic retreatment that has been filled due to the leakage of apical and coronal. One stage in this retreatment is an uptake of obturasi material using hedstrom file (H-file). The solvent material used in this endodontic retreatment is xylol. Case report. A 22-year-old male patient came to the Clinic of Conservative Dentistry Dental Hospital Prof. Soedomo, Faculty of Dentistry, Gadjah Mada University (UGM) wanted to treat his mandibular second right premolar (45) that has been painful since two months ago. The tooth had root canal treatment one year ago. The pain suddenly appears and disappears as soon as the patient consumes painkillers. Dental preoperative (periapical) radiograph tooth 45 showed a picture of gutta percha root canals and radiolucent images at the tip of the root of the tooth. Diagnosis of tooth 45 is non-vital tooth after root canal treatment accompanied by apical, symptomatic periodontitis. The operator performed a root canal treatment by dismantling the restoration on the occlusal part of tooth 45 and taking gutta percha using a hedstrom file followed by treatment of the root canal, crown lengthening, installation of individual formable fiber post and porcelain fused to metal crown. Conclusion. Retreatment of root canal treatment with non-surgical methods still can be conducted effectively and obtain good results with the final treatment results in smaller lesions in the apical portion of tooth 45.

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