A sensitive colorimetric immunoassay was established for quantitative detection of E. coli O157:H7. Au@Pt with peroxidase-like activity was combined with flaky reduced graphene oxide to form a chromogenic probe of pseudo enzyme. HRP was modified on rGO-NR–Au@Pt and a dual signal amplification system was realized.
In this work, a sensitive and selective electrochemical aptasensor for detection of microcystin-LR was developed based on a dual signal amplification system consisted of a novel ternary composite and horseradish peroxidase.