Assessment of Annona reticulata Linn. leaves fractions for invitro antioxidative effect and antimicrobial potential against standard human pathogenic strains

A series of diazenyl chalcones was prepared by base catalyzed Claisen-Schmidt condensation of synthesized hydroxy substituted acetophenone azo dye with various substituted aromatic/ heteroaromatic aldehydes. The structural conformation of synthesized chalcones was done by a number of physicochemical and spectral means like FTIR, UV-visible, mass, NMR spectroscopy and CHNS/O analysis. These diazenyl chalcones were assessed for their in vitro antimicrobial potential against several Gram-negative, Gram-positive bacterial and fungal strains by serial tube dilution method. The fluconazole and cefadroxil were used as standard drugs. The target compounds were also evaluated for their antioxidant potential by DPPH assay. (2E)-3-(2,4-Dichlorophenyl)-1-(4-((2,6- dihydroxyphenyl)diazenyl)phenyl)prop-2-en-1-one (C-7) had shown very good antimicrobial potential with MIC ranges from 3.79 to 15.76 μg/ml against most of the tested microorganisms. Most of the synthesized diazenyl chalcones were found to be active against B. subtilis. The (2E)-1-(5-((2-Chloro- 4-nitrophenyl)diazenyl)-2-hydroxyphenyl)-3-(2-hydroxynaphthalen-1-yl)prop-2-en-1-one (C-10) had shown high free radical-scavenging activity when compared with the ascorbic acid as the reference antioxidant.

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Phytochemical Constituents of Annona reticulata and their Cytotoxic Activity

Letters in Organic Chemistry ◽

10.2174/1570178616666190724123004 ◽

2020 ◽

Vol 17 (3) ◽

pp. 206-210

Author(s):

Ty Viet Pham ◽

Thang Quoc Le ◽

Anh Tuan Le ◽

Hung Quoc Vo ◽

Duc Viet Ho

Keyword(s):

Cytotoxic Activity ◽

Cell Lines ◽

Structural Determination ◽

Phytochemical Investigation ◽

Ic50 Values ◽

Phytochemical Constituents ◽

First Time ◽

Annona Reticulata

A phytochemical investigation of the leaves of Annona reticulata led to the isolation and structural determination of β-sitosterol (1), ent-pimara-8(14),15-dien-19-oic acid (2), ent-pimara- 8(14),15-dien-19-ol (3), quercetin (4), quercetin 3-O-α-L-arabinopyranoside (5), and a mixture of quercetin 3-O-β-D-galactopyranoside (6a) and quercetin 3-O-β-D-glucopyranoside (6b). Of these, compounds 2 and 3 were isolated from the genus Annona for the first time. Compound 3 showed strong cytotoxicity against SK-LU-1 and SW626 cell lines with IC50 values of 17.64 ± 1.07 and 19.79 ± 1.41 μg mL-1, respectively.

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Total Phenolic Content, Volatile Constituents and Antioxidative Effect of Coriandrum sativum, Murraya koenigii and Mentha arvensis

The Natural Products Journal ◽

10.2174/2210315506666161121104251 ◽

2017 ◽

Vol 7 (1) ◽

pp. 65-74

Author(s):

Pandian Arjun ◽

Deepak Semwal ◽

Ruchi Semwal ◽

Malaiyandi Malaisamy ◽

Chandrasekaran Sivaraj ◽

...

Keyword(s):

Total Phenolic Content ◽

Phenolic Content ◽

Coriandrum Sativum ◽

Total Phenolic ◽

Murraya Koenigii ◽

Mentha Arvensis ◽

Antioxidative Effect ◽

Volatile Constituents

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Evaluation of Anti-diabetic and Anti-hyperlipidemic Activity of Isolated Bioactive Compounds of Leaves of Annona reticulata Linn.

The Natural Products Journal ◽

10.2174/2210315510999200511132940 ◽

2020 ◽

Vol 10 ◽

Author(s):

Kalyani Pathak ◽

Aparoop Das ◽

Anshul Shakya ◽

Riya Saikia ◽

Himangshu Sarma

Keyword(s):

Spectral Analysis ◽

Gallic Acid ◽

Liver Enzymes ◽

Serum Glucose ◽

Diabetic Rats ◽

Traditional Use ◽

Per Oral ◽

Diabetes And Obesity ◽

Annona Reticulata

Background: The leaves of Annona reticulata Linn. have been traditionally used by the tribes of Assam as a source of medicine to mitigate a range of health ailments including diabetes and obesity. Objectives: The current study aimed to evaluate the anti-diabetic and anti-hyperlipidemic potential of bioactive fractions isolated from the methanolic extract of Annona reticulata Linn. leaves using Nicotinamide + Streptozotocin (60 mg/kg, i.p.) induced diabetic rats. Methods: The partially purified bioactive fractions, namely F1, F2, F3 and F4 were administered to diabetic rats with the dose of 200 mg/kg, per oral (p.o.) and the effect of the fractions on serum glucose were studied up to 21 days. The potent fractions were further subjected for spectral analysis for identification of the isolated active compounds. Results: The in-vivo anti-diabetic activity of the isolated fractions F2 and F3 were found significant controlling blood glucose level, alike glibenclamide. Interestingly, F2 and F3 treated animals were found significant in restoring the lipid and liver enzymes profile in streptozotocin challenge rats. Further, spectral analysis revealed that F2 and F3 were comprises Quercetin and Gallic acid, respectively. Conclusion: Outcome of finding demonstrate the anti-diabetic and anti-hyperlipidemic potential of the isolates/fractions of A. reticulata, which were found enriched in polyphenolics including Quercetin and Gallic acid; and provides logistic behind the traditional use of the A. reticulata against Diabetes and obesity.

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