scholarly journals Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

2016 ◽  
Vol 216 ◽  
pp. 1-18 ◽  
Author(s):  
D.R. Yáñez-Ruiz ◽  
A. Bannink ◽  
J. Dijkstra ◽  
E. Kebreab ◽  
D.P. Morgavi ◽  
...  
Keyword(s):  
Batch Culture ◽  
Methane Mitigation ◽  
Enteric Methane

scholarly journals Enteric methane mitigation and fermentation kinetics of forage species from Southern Mexico: in vitro screening

2021 ◽  
Author(s):  
Sara S. Valencia-Salazar ◽  
Guillermo Jiménez-Ferrer ◽  
Jacobo Arango ◽  
Isabel Molina-Botero ◽  
Ngonidzashe Chirinda ◽  
...  
Keyword(s):  
In Vitro Screening ◽  
Southern Mexico ◽  
Methane Mitigation ◽  
Fermentation Kinetics ◽  
Forage Species ◽  
Enteric Methane ◽  
Kinetics Of

scholarly journals Impact of 2′-Fucosyllactose on Gut Microbiota Composition in Adults with Chronic Gastrointestinal Conditions: Batch Culture Fermentation Model and Pilot Clinical Trial Findings

Nutrients ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 938
Author(s):  
Jennifer Joan Ryan ◽  
Andrea Monteagudo-Mera ◽  
Nikhat Contractor ◽  
Glenn R. Gibson
Keyword(s):  
Ulcerative Colitis ◽  
Batch Culture ◽  
Pilot Trial ◽  
Gastrointestinal Symptoms ◽  
Short Chain Fatty Acids ◽  
Open Label ◽  
Gastrointestinal Conditions ◽  
Fermentation Model ◽  
Quality Of Life Index

Intestinal dysbiosis has been described in patients with certain gastrointestinal conditions including irritable bowel syndrome (IBS) and ulcerative colitis. 2′-fucosyllactose (2′-FL), a prebiotic human milk oligosaccharide, is considered bifidogenic and butyrogenic. To assess prebiotic effects of 2′-FL, alone or in combination with probiotic strains (potential synbiotics), in vitro experiments were conducted on stool from healthy, IBS, and ulcerative colitis adult donors. In anaerobic batch culture fermenters, Bifidobacterium and Eubacterium rectale-Clostridium coccoides counts, and short-chain fatty acids (SCFAs) including butyrate increased during fermentation with 2′-FL and some of the 2′-FL/probiotic combinations. In a subsequent open-label pilot trial, the effect of a 2′-FL-containing nutritional formula was evaluated in twelve adults with IBS or ulcerative colitis. Gastrointestinal Quality of Life Index (GIQLI) total and gastrointestinal symptoms domain scores, stool counts of Bifidobacterium and Faecalibacterium prausnitzii, and stool SCFAs including butyrate, increased after six weeks of intervention. Consistent with documented effects of 2′-FL, the batch culture fermentation experiments demonstrated bifidogenic and butyrogenic effects of 2′-FL during fermentation with human stool samples. Consumption of the 2′-FL-containing nutritional formula by adults with IBS or ulcerative colitis was associated with improvements in intra- and extra-intestinal symptoms, and bifidogenic and butyrogenic effects.

scholarly journals Reducing enteric methane production from buffalo (Bubalus bubalis) by garlic oil supplementation in in vitro rumen fermentation system

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Avijit Dey ◽  
Shyam Sundar Paul ◽  
Puran Chand Lailer ◽  
Satbir Singh Dahiya
Keyword(s):  
Environmental Pollution ◽  
Methane Production ◽  
Rumen Fluid ◽  
Bubalus Bubalis ◽  
Rumen Fermentation ◽  
Acetyl Esterase ◽  
Garlic Oil ◽  
Enteric Methane ◽  
In Vitro Rumen Fermentation

AbstractEnteric methane production contributes significantly to the greenhouse gas emission globally. Although, buffaloes are integral part of livestock production in Asian countries, contributing milk, meat and draft power, the contribution of enteric methane to environmental pollution attracts attention. The present study investigated the efficacy of garlic (Allium sativum) oil in reducing enteric methane production from buffaloes (Bubalus bubalis) by in vitro rumen fermentation. Garlic oil (GOL) was tested at four concentrations [0 (Control), 33.33 µl (GOL-1), 83.33 µl (GOL-2) and 166.66 µl (GOL-3) per litre of buffered rumen fluid] in 100-ml graduated glass syringes and incubated at 39℃ for 24 h for in vitro rumen fermentation study. Supplementation of GOL-1 increased (p < 0.05) total gas production in comparison with GOL-3; however, it remained comparable (p > 0.05) with control and GOL-2. Graded doses of garlic oil inclusions reduced (p < 0.001) methane concentration (%) in total gas and total methane production (ml/g DM), irrespective of concentrations. The feed degradability, volatile fatty acids and microbial biomass production (MBP) were not affected (p > 0.05) by GOL-1, but these tended to decrease in GOL-2 with marked reduction (p < 0.01) in GOL-3. The decrease (p < 0.01) in NH3–N concentration in fermentation fluid in the presence of garlic oil, irrespective of concentration, suggests reduced deamination by inhibiting rumen proteolytic bacterial population. The activities of ruminal fibrolytic enzymes (CMCase, xylanase, β-glucosidase, acetyl esterase) were not affected by lower dose (GOL-1) of garlic oil; however, reduction (p < 0.05) of these enzymes activity in rumen liquor was evident at higher doses (GOL-2 and GOL-3) of supplementation. This study shows positive impact of garlic oil supplementation at low dose (33.33 µl/l of rumen fluid) in reducing enteric methane production, thereby, abatement of environmental pollution without affecting feed digestibility.

Methanotrophs in enteric methane mitigation.

2015 ◽  
pp. 360-375 ◽  
Author(s):  
N. M. Soren ◽  
P. K. Malik ◽  
V. Sejian
Keyword(s):  
Methane Mitigation ◽  
Enteric Methane

In vitro methane production as influenced by harvest date and level of nitrogen application

2003 ◽  
Vol 2003 ◽  
pp. 163-163
Author(s):  
D. K. Lovett ◽  
A. Bortolozzo ◽  
P. O’Kiely ◽  
P. Conaghan ◽  
F. P. O’Mara
Keyword(s):  
Methane Production ◽  
Production Systems ◽  
Livestock Production ◽  
Harvest Date ◽  
Nitrogen Application ◽  
N Level ◽  
Grazed Pasture ◽  
Enteric Methane ◽  
Fertiliser Application

Increasing animal productivity increases daily enteric methane (CH4) emissions but reduces CH4 output per unit of animal production. Irish livestock production systems are characterised by a high dependence on grazed pasture. Increased nitrogen (N) fertiliser application can increase animal productivity through increased intake. The objective of this trial was to assess the effect of N level application and pasture maturity on in vitro methane production.

Tropical forage trees with low potential defaunating capacity

2005 ◽  
Vol 2005 ◽  
pp. 221-221
Author(s):  
G. E. Monforte Briceño ◽  
C. A. Sandoval Castro ◽  
C. M. Capetillo Leal ◽  
L. Ramírez Avilés
Keyword(s):  
Batch Culture ◽  
Culture System ◽  
Potential Effect ◽  
Rumen Protozoa ◽  
Tropical Forage ◽  
Livestock Feeding ◽  
The Tropics ◽  
Tropical Systems

Forage trees are commonly use for livestock feeding in the tropics. It is known that some species can affect the rumen protozoa population (Odenyoet al., 1997). However, little is known about the potential effect upon rumen protozoa of several species which are also use as feed in tropical systems. The objective of the experiment was to assess the defaunating capacity of forage trees. In companion reports (Monforteet al., 2005) we reported plants with a potential defaunating effect as evaluated under an in vitro batch culture system (Sandovalet al., 2005). Here we present those plants which did not have or had low effect on protozoa population in anin vitroculture.

616 Effect of conservation method on in vitro ruminal fermentation of purple prairie clover (Dalea purpurea Vent.) in batch culture

2017 ◽  
Vol 95 (suppl_4) ◽  
pp. 301-302
Author(s):  
K. Peng ◽  
Z. Xu ◽  
L. Jin ◽  
T. A. McAllister ◽  
S. Acharya ◽  
...  
Keyword(s):  
Batch Culture ◽  
Ruminal Fermentation ◽  
Conservation Method

scholarly journals 190 Reduce Reuse ReRumen: Preservation method of rumen fluid collected from slaughtered cattle affects in vitro digestibility

2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 66-67
Author(s):  
Taylor J Garcia ◽  
Jeffrey A Brady ◽  
Kimberly A Guay ◽  
James P Muir ◽  
William B Smith
Keyword(s):  
Batch Culture ◽  
Rumen Fluid ◽  
Chemical Constituents ◽  
Rumen Content ◽  
In Vitro Digestibility ◽  
Constant Weight ◽  
Preservation Methods ◽  
Preservation Method ◽  
Pass Through

Abstract Microbes and chemical constituents in the rumen fluid play an important role when re-establishing the rumen microbial population. Identification of a viable preservation method could have lasting implication of veterinary and research applications. Our objective was to determine the efficacy of preservation methods on rumen liquor. Twelve paunch samples were collected from slaughtered cattle at the Tarleton State University Meat Laboratory, Stephenville, TX. Rumen content was collected from different locations within the rumen, strained through eight layers of cheesecloth, and divided into five 500-mL aliquots. Aliquots were randomly allocated to one of five preservation methods (freezing or lyophilisation) and preservative (glycerol; yes or no). A fresh control was maintained from each sample. Three reference feeds and two roughage sources were used in the batch culture procedure. Reference feeds were dried to a constant weight at 55°C and ground to pass through a 2-mm screen. Ground samples were subjected to batch culture in vitro true digestibility (IVTD) assays using each of the four ruminal fluids (plus the control) for digestibility estimates. Original samples and incubated residues were assayed for DM, CP, NDF, and ADF. Data were analyzed in a 2 × 2 + 1 augmented factorial design using GLIMMIX procedure of SAS. There were no differences (P > 0.05) in IVTD when using protein or energy feeds; however, there were differences in IVTD for all treatments for fiber concentrates and forage reference feeds. Despite lower degradation of feeds when frozen or lyophilized rumen fluid was used, preserved rumen fluid may be a viable option in the absence of fresh inoculum for veterinary and research applications

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