SHORT COMMUNICATION: Evaluating the relationship between in vitro and in situ starch degradation rates

2021 ◽  
pp. 115175
Author(s):  
C.B. Gleason ◽  
L.M. Beckett ◽  
B.R. dos Reis ◽  
R.R. White
Keyword(s):  
Short Communication ◽  
Starch Degradation ◽  
Degradation Rates ◽  
The Relationship

scholarly journals In vitro estimation of rumen fermentable organic matter using enzymes

1996 ◽  
Vol 44 (2) ◽  
pp. 103-110 ◽  
Author(s):  
J.W. Cone ◽  
A.H. Van Gelder ◽  
A.M. Van Vuuren
Keyword(s):  
Organic Matter ◽  
Crude Fibre ◽  
Fermentation Products ◽  
In Situ Method ◽  
Degradation Rates ◽  
Digestible Organic Matter ◽  
Similar Accuracy ◽  
The Relationship

The amount of rumen fermentable organic matter (FOM) can be calculated using tables, taking into account the amount of digestible organic matter, the content of fat and fermentation products, and the amount of starch and protein escaping rumen fermentation, or FOM can be calculated using in situ incubations. An in vitro method is described to predict FOM using amylase and other carbohydrate degrading enzymes. FOM estimated by the enzymic method showed a moderate correlation (Rsuperscript 2 = 0.71) with FOM estimated by the in situ method. The relationship could be improved by separating the high crude fibre samples (Rsuperscript 2 = 0.88) from the other samples (Rsuperscript 2 = 0.77). Because degradation rates with the enzymic method were high compared with the assumed rumen passage rates, it proved that FOM could be predicted with a similar accuracy (Rsuperscript 2 = 0.76 - 0.80) by the undegraded fraction after 24 h.

Chemical characteristics and in situ ruminal parameters of barley for cattle: Comparison of the malting cultivar AC Metcalfe and five feed cultivars

2008 ◽  
Vol 88 (4) ◽  
pp. 711-719 ◽  
Author(s):  
Kenton J Hart ◽  
Brian G Rossnagel ◽  
Peiqiang Yu
Keyword(s):  
Nutritive Value ◽  
Degradation Kinetics ◽  
Starch Degradation ◽  
Barley Cultivars ◽  
Degradation Rates ◽  
Key Words Barley ◽  
In Situ Degradation ◽  
The Mean ◽  
Pass Through

The objective of this study was to compare the most widely grown barley cultivar in Canada, AC Metcalfe, a malting type barley, with five feed cultivars. Barley cultivars were grown at one location during 3 consecutive years and barley samples were milled to pass through a 1-mm screen and analysed to determine nutritive value. Additional samples were passed through a roller mill with a gap set at 1.12 mm and incubated ruminally for 0, 2, 4, 8, 12, 24, and 48 h in 3 dry Holstein cows fitted with rumen cannulae. The rate and extent of rumen digestion were estimated. AC Metcalfe had a higher (P < 0.001) concentration of NDF, and lower (P < 0.05) concentrations of non structural carbohydrates, starch, ADF, total digestible nutrients, and fermentable cell wall carbohydrates compared with the mean of the feed cultivars. The malting cultivar had a higher (P < 0.001) soluble DM fraction, lower (P < 0.05) CP and starch degradation rates, and a lower (P < 0.001) ruminally degradable starch concentration compared with the mean of the five feed cultivars. The results demonstrate that there are only small differences in terms of chemical composition and in situ degradation kinetics between the malting cultivar AC Metcalfe and the five feed cultivars of barley reported here. Key words: Barley, energy, protein, ruminants

The relationship between erythropoietin-dependent cellular differentiation and colony-forming ability in prenatal haemopoietic tissues

Development ◽  
1975 ◽  
Vol 34 (3) ◽  
pp. 575-588
Author(s):  
R. J. Cole ◽  
T. Regan ◽  
S. L. White ◽  
E. M. Cheek
Keyword(s):  
Cellular Differentiation ◽  
Cell Numbers ◽  
In Vitro Sensitivity ◽  
Foetal Liver ◽  
Rapid Production ◽  
Colony Forming Cell ◽  
Macrophage Colony ◽  
The Relationship

Levels of haem synthesis achieved by foetal liver erythroblasts responding to erythropoietin in vitro are similar in dissociated cell cultures and in cultures of organized tissues. Erythroid colony-forming cells reach maximum numbers on the sixteenth day of gestation. Their presence in foetal liver is associated with the period of most rapid production of erythrocytes, and with in vitro sensitivity to erythropoietin measured as enhanced haem synthesis. It is concluded that at least a proportion of erythroid colony-forming cells in the foetal liver are dependent on erythropoietin in situ and that these cells are separated from the earliest recognizable pro-erythroblast by 1–2 cell divisions. Populations of granulocyte-macrophage colony-forming cells change independently of erythroid colony-forming cell numbers.

The relationship between in vitro and in situ dry matter disappearance of some Iranian feedstuffs in sheep

2007 ◽  
Vol 2007 ◽  
pp. 210-210
Author(s):  
H. Paya ◽  
A. Taghizadeh ◽  
H. Janmohamadi ◽  
G.A Moghadam
Keyword(s):  
Dry Matter ◽  
Nutrient Requirements ◽  
Feed Ingredients ◽  
The Relationship ◽  
Require Information

Ration formulation systems require information on nutrient requirements of the animal and reliable values for rumen degradable and undegradable fractions of feed ingredients. The in situ nylon-bag technique is widely used to characterize the disappearance of feeds from the rumen (Woods et al., 2002). The objective of this study was determining of relationship between in vitro and in situ dry matter disappearance.

scholarly journals Short communication: Sodium salicylate negatively affects rumen fermentation in vitro and in situ

2017 ◽  
Vol 100 (3) ◽  
pp. 1935-1939 ◽  
Author(s):  
A.J. Carpenter ◽  
C.F. Vargas Rodriguez ◽  
J.A.B. Jantz ◽  
B.J. Bradford
Keyword(s):  
Short Communication ◽  
Sodium Salicylate ◽  
Rumen Fermentation

scholarly journals miR-375 mediates CRH signaling pathway in inhibiting E2 synthesis in porcine ovary

Reproduction ◽  
2017 ◽  
Vol 153 (1) ◽  
pp. 63-73 ◽  
Author(s):  
Chulin Yu ◽  
Meiling Li ◽  
Yue Wang ◽  
Ying Liu ◽  
Chengzhi Yan ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Granulosa Cells ◽  
Reproductive System ◽  
Follicular Development ◽  
Cell Types ◽  
Signaling System ◽  
Key Factor ◽  
The Relationship

The corticotropin-releasing hormone (CRH) signaling system is involved in numbers of stress-related physiological and pathological responses, including its inhibiting effects on estradiol (E2) synthesis and follicular development in the ovary. In addition, there are reports that microRNAs (miRNAs) can control the function of animal reproductive system. The aim of present study was to investigate the functions of miR-375 and the relationship between miR-375 and CRH signaling molecules in the porcine ovary. First, our common PCR results show that miR-375 and the CRH receptor 1 (CRHR1) are expressed in porcine ovary, whereas CRH receptor 2 (CRHR2) is not detected. We further have located the cell types of miR-375 and CRHR1 by in situ hybridization (ISH), and the results show that miR-375 is located only in the granulosa cells, whereas CRHR1 is positive in all of granulosa cells and oocytes, inferring that miR-375 and CRHR1 are co-localized in granulosa cells. Second, we show that overexpression of miR-375 in cultured granulosa cells suppresses the E2 production, whereas miR-375 knockdown demonstrates the opposite result. Besides, our in vitro results demonstrate that miR-375 mediates the signaling pathway of CRH inhibiting E2 synthesis. Finally, our data show that the action of miR-375 is accomplished by directly binding to the 3′UTR of specificity protein1 (SP1) mRNA to decrease the SP1 protein level. Thus, we conclude that miR-375 is a key factor in regulating E2 synthesis by mediating the CRH signaling pathway.

scholarly journals In situ and in vitro ruminal starch degradation of untreated and formaldehyde-treated wheat and maize

1997 ◽  
Vol 37 (3) ◽  
pp. 305-312 ◽  
Author(s):  
B. Michalet-Doreau ◽  
C. Philippeau ◽  
M. Doreau
Keyword(s):  
Starch Degradation

Short Communication: The effect of seed hardness and malting characteristics on in situ dry matter digestibility of barley grain in beef heifers

2015 ◽  
Vol 95 (2) ◽  
pp. 299-303 ◽  
Author(s):  
S. Ding ◽  
M. Oba ◽  
M. L. Swift ◽  
M. Edney ◽  
J. T. O'Donovan ◽  
...  
Keyword(s):  
Dry Matter ◽  
Short Communication ◽  
Barley Grain ◽  
Beef Heifers ◽  
Beta Glucan ◽  
Diastatic Power ◽  
Dry Matter Digestibility ◽  
Seed Hardness ◽  
The Relationship

Ding, S., Oba, M., Swift, M. L., Edney, M., O'Donovan, J. T., McAllister, T. A. and Yang, W. Z. 2015. Short Communication: The effect of seed hardness and malting characteristics on in situ dry matter digestibility of barley grain in beef heifers. Can. J. Anim. Sci. 95: 299–303. An in situ study was conducted to evaluate the relationship between ruminal dry matter digestibility (DMD) and seed hardness or malting characteristics of barley grain. Samples were selected for low and high values of seed hardness index (53 vs. 65; N = 18), beta-glucan content in wort (122 vs. 316 ppm; N = 18), diastatic power (146 vs. 203°L; N = 18), and friability (46 vs. 81%; N = 18) in malt, and incubated in the rumen of three beef heifers for 4, 12 and 48 h. In situ DMD did not vary with beta-glucan concentration or friability. However, barley grain with low seed hardness had lower (P = 0.02) in situ DMD than those with high seed hardness after 4 h of incubation. The barley samples with low diastatic power also had (P = 0.02) higher DMD than with high diastatic power after 4 h, a trend (P = 0.07) that continued after 12 h of incubation. Seed hardness and malting characteristics may have the potential to predict DMD of barley grain in the rumen. However, observed differences in in situ DMD were relatively minor, and we did not detect a relationship between malting characteristics and in situ DMD at longer incubation times. This suggests that the identified grain physical and malt parameters may impact the rate, but not the extent of barley grain digestion in the rumen.

Comparison of starch degradation in cereal grains by incubation in vitro with rumen fluid and boiling with α-amylase solution

2007 ◽  
Vol 2007 ◽  
pp. 23-23
Author(s):  
C. M. Guedes ◽  
M. M. Rodrigues ◽  
A. Oliveira ◽  
A. L. Lourenço ◽  
S. R. Silva ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Dairy Cow ◽  
Rumen Fluid ◽  
New Method ◽  
Cereal Grains ◽  
Starch Degradation ◽  
Available Energy

Cereal grains are the most common sources of readily available energy for dairy cows. Several in vitro and in situ studies have measured starch degradability of cereal grains. However, most of these studies compared few samples and grain species and used only one method. Compared with protein, much less information is available on this topic. Such information would allow a more efficient use of energy and protein in dairy cow diets. The objective of this study was to determine starch degradation of 32 samples of 7 cereal grains using in vitro incubation with rumen fluid and a new method based on a boiling extraction with an enzymatic (thermo stable α-amylase) solution.

Rumen degradability of Mongolian pastures: a comparison of in situ and in vitro gas production techniques

1996 ◽  
Vol 1996 ◽  
pp. 227-227 ◽  
Author(s):  
A.H. Murray ◽  
D. Daalkhaijav ◽  
C.D. Wood
Keyword(s):  
Gas Production ◽  
Forest Steppe ◽  
High Mountain ◽  
In Vitro Gas Production ◽  
Production Techniques ◽  
Native Pastures ◽  
The Relationship

In Mongolia animal performance is very much dependent on the quality and quantity of natural grassland available, since certain pastoral animals may obtain as much as 98% of their annual intake from pasture. There have been few studies to date on either in vivo or in vitro degradation of native Mongolian pastures. This paper seeks to investigate the degradation characteristics of native pastures from 2 regions in Mongolia, high mountain and forest steppe. It also investigates the relationship between the in vitro gas production technique and the in sacco technique.

Sign in / Sign up

Export Citation Format

Share Document