Effect of starvation on the antioxidative pathway, autophagy, and mitochondrial function in the intestine of Chinese perch Siniperca chuatsi

Aquaculture ◽  
2021 ◽  
pp. 737683
Author(s):  
Yaxiong Pan ◽  
Jinsheng Tao ◽  
Jun Zhou ◽  
Jia Cheng ◽  
Yuanhua Chen ◽  
...  
Keyword(s):  
Mitochondrial Function ◽  
Siniperca Chuatsi ◽  
Chinese Perch

scholarly journals Large-Scale Microcarrier Culture of Chinese Perch Brain Cell for Viral Vaccine Production in a Stirred Bioreactor

Vaccines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1003
Author(s):  
Xia Luo ◽  
Yinjie Niu ◽  
Xiaozhe Fu ◽  
Qiang Lin ◽  
Hongru Liang ◽  
...  
Keyword(s):  
Culture System ◽  
Large Scale ◽  
Brain Cell ◽  
Vaccine Production ◽  
Mandarin Fish ◽  
Siniperca Chuatsi ◽  
Stirred Bioreactor ◽  
Viral Vaccine ◽  
Chinese Perch ◽  
Large Scale Culture

Mandarin fish (Siniperca chuatsi) is one of the important cultured fish species in China. Infectious spleen and kidney necrosis virus (ISKNV) and Siniperca Chuatsi rhabdovirus (SCRV) have hindered the development of mandarin fish farming industry. Vaccination is the most effective method for control of viral diseases, however viral vaccine production requires the large-scale culture of cells. Herein, a suspension culture system of Chinese perch brain cell (CPB) was developed on Cytodex 1 microcarrier in a stirred bioreactor. Firstly, CPB cells were cultured using Cytodex 1 microcarrier in 125 mL stirring flasks. With the optimum operational parameters, CPB cells grew well, distributed uniformly, and could fully cover the microcarriers. Then, CPB cells were digested with trypsin and expanded step-by-step with different expansion ratios from the 125 mL stirring bottle to a 500 mL stirring bottle, and finally to a 3-L bioreactor. Results showed that with an expansion ratio of 1:3, we achieved a high cell density level (2.25 × 106 cells/mL) with an efficient use of the microcarriers, which also confirmed the data obtained from the 125 mL stirring flask. Moreover, obvious cytopathic effects (CPE) were observed in the suspended CPB cells post-infection with ISKNV and SCRV. This study provided a large-scale culture system of CPB cells for virus vaccine production.

scholarly journals Identification and profiling of growth-related microRNAs in Chinese perch (Siniperca chuatsi)

BMC Genomics ◽  
2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Jiagang Tu ◽  
Changxu Tian ◽  
Peiqi Zhao ◽  
Junxiao Sun ◽  
Min Wang ◽  
...  
Keyword(s):  
Siniperca Chuatsi ◽  
Chinese Perch

Differences of gut microbiota and lipid metabolism in Chinese perch ( Siniperca chuatsi ) with different growth rates

2021 ◽  
Author(s):  
Yanpeng Zhang ◽  
Hexiong Feng ◽  
Xu‐Fang Liang ◽  
Shan He ◽  
Qiwei Zhang ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Gut Microbiota ◽  
Growth Rates ◽  
Siniperca Chuatsi ◽  
Chinese Perch

Role of NPY receptor 8 in regulating of food intake in Chinese perch (Siniperca chuatsi)

2021 ◽  
Author(s):  
Yanpeng Zhang ◽  
Zhen Zhang ◽  
Xu-Fang Liang ◽  
Shan He ◽  
Jing Xu
Keyword(s):  
Food Intake ◽  
Siniperca Chuatsi ◽  
Npy Receptor ◽  
Chinese Perch
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