Reproductive toxicity of inorganic mercury exposure in adult zebrafish: Histological damage, oxidative stress, and alterations of sex hormone and gene expression in the hypothalamic-pituitary-gonadal axis

2016 ◽  
Vol 177 ◽  
pp. 417-424 ◽  
Author(s):  
Qun-Fang Zhang ◽  
Ying-Wen Li ◽  
Zhi-Hao Liu ◽  
Qi-Liang Chen
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Reproductive Toxicity ◽  
Inorganic Mercury ◽  
Mercury Exposure ◽  
Sex Hormone ◽  
Adult Zebrafish ◽  
Histological Damage

scholarly journals Protective Effect of Hydroalcoholic Extract of Orange Peel on PCNA and FSH-R Gene Expression in Histological Damage and Oxidative Stress Due to Ovarian Torsion in Adult Rats

2021 ◽  
Vol 9 (3) ◽  
pp. 205-211
Author(s):  
Seyedeh-Roza Tafrishi Nejad ◽  
Arash Khaki ◽  
Shamci Abbasalizadeh ◽  
Majid Shokoohi ◽  
Nava Ainehchi
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Protective Effect ◽  
Ovarian Tissue ◽  
Orange Peel ◽  
Ovarian Torsion ◽  
R Gene ◽  
Adult Rats ◽  
Hydroalcoholic Extract ◽  
Histological Damage

Objectives: The aim of this study was to evaluate the protective effect of the hydroalcoholic extract of orange peel on proliferating cell nuclear antigen (PCNA) and follicle-stimulating hormone receptor (FSH-R) gene expression in histological injuries and acid stress caused by ovarian torsion in adult rats. Materials and Methods: In this experimental study, 32 adult female rats were randomly divided into 4 groups. In group 1 (Sham), the abdominal wall was cut without applying torsion and in group 2, ovarian torsion was performed for 2 hours, followed by detorsion for 2 weeks. The hydro-alcoholic extract of orange peel was added to their diet for two weeks in group 3, followed by ovarian torsion for 2 hours and detorsion for 2 hours. Group 4 received the orange peel extract for two weeks and after then ovarian resection for the evaluation of histological damage and blood sampling to examine the serum level of antioxidant enzymes, as well as the expression of PCNA and FSH-R genes in the ovarian tissue. Results: Histological changes in the ovary tissue of rats showed that torsion and detorsion have destructive effects on the ovarian tissue, and torsion/detorsion led to a reduction in the expression of PCNA and FSH-R (P < 0.05). Based on biochemical and hormonal results, the ovarian torsion resulted in an imbalance in the oxidative stress markers and hormone profile of rats. Finally, the administration of the hydroalcoholic extract of orange peel due to its high antioxidant properties improves these effects. Conclusions: In general, administering an appropriate dose of the hydroalcoholic extract of orange peel for two consecutive weeks in the diet had a protective effect on the ovarian tissue at the risk of torsion/detorsion.

8. Does Oxidative Stress Function as a Biomarker of Environmental Mercury Contamination in Double-Crested Cormorant?

2018 ◽  
Author(s):  
Laura Gibson
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Mercury Concentration ◽  
Dry Weight ◽  
Threshold Concentration ◽  
Mercury Exposure ◽  
Mercury Contamination ◽  
Gavia Immer ◽  
Wide Range ◽  
And Oxidative Stress

Mercury is a widespread contaminant that has been shown to induce a wide range of adverse health effects in several animal species including changes in gene expression. This study was undertaken to explore the relationship between environmental mercury exposure and oxidative stress induction in the blood of double-crested cormorants (Phalacrocorax auritus). Blood was sampled at five sites across Central and Southern Ontario ranging from low to high risk of mercury contamination and was analyzed for total mercury concentration. To assess cellular oxidative stress, the expression of glutathione peroxidases 1 and 3, glutathione S-transferase µ3, superoxide dismutase 1 and heat-shock protein 70 kd-8 were measured in whole blood samples. Preliminary results indicate that erythrocyte mercury concentrations ranged from 5.0 to 27.3 µg/g dry weight. Mean (± standard error) site-specific erythrocyte mercury concentrations ranged from 9.0 ± 3.6 µg/g dry weight in Lake Erie to 17.5 ± 4.2 µg/g dry weight in Lake Nipissing. Approximately 27% of the birds exceeded the threshold concentration for adverse effects in another piscivorous bird, the common loon (Gavia immer). Results of gene expression analysis will be discussed in relation to mercury concentration. Our study will help to develop an understanding of the interactions of environmental mercury exposure and oxidative stress-related gene expression and will contribute to the development of sub lethal biomarkers for mercury toxicity in wild aquatic piscivorous birds.

Chemoprotective Effects of Propolis on Aflatoxin B1-Induced Hepatotoxicity in Rats: Oxidative Damage and Hepatotoxicity by Modulating TP53, Oxidative Stress

2020 ◽  
Vol 17 (3) ◽  
pp. 191-199
Author(s):  
Seval Yilmaz ◽  
Fatih Mehmet Kandemir ◽  
Emre Kaya ◽  
Mustafa Ozkaraca
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Oxidative Damage ◽  
Aflatoxin B1 ◽  
Tp53 Gene ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Gene Expressions ◽  
Cat Gene ◽  
Gst Activity

Objective: This study aimed to detect hepatic oxidative damage caused by aflatoxin B1 (AFB1), as well as to examine how propolis protects against hepatotoxic effects of AFB1. Method: Rats were split into four groups as control group, AFB1 group, propolis group, AFB1+ propolis group. Results: There was significant increase in malondialdehyde (MDA) level and tumor suppressor protein (TP53) gene expression, Glutathione (GSH) level, Catalase (CAT) activity, CAT gene expression decreased in AFB1 group in blood. MDA level and Glutathione-S-Transferase (GST) activity, GST and TP53 gene expressions increased in AFB1 group, whereas GSH level and CAT activity alongside CAT gene expression decreased in liver. AFB1+propolis group showed significant decrease in MDA level, GST activity, TP53 and GST gene expressions, GSH level and CAT activity and CAT gene expression increased in liver compared to AFB1 group. Conclusion: These results suggest that propolis may potentially be natural agent that prevents AFB1- induced oxidative stress and hepatotoxicity.

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