Protective Effect of Hydroalcoholic Extract of Orange Peel on PCNA and FSH-R Gene Expression in Histological Damage and Oxidative Stress Due to Ovarian Torsion in Adult Rats

Objectives: The aim of this study was to evaluate the protective effect of the hydroalcoholic extract of orange peel on proliferating cell nuclear antigen (PCNA) and follicle-stimulating hormone receptor (FSH-R) gene expression in histological injuries and acid stress caused by ovarian torsion in adult rats. Materials and Methods: In this experimental study, 32 adult female rats were randomly divided into 4 groups. In group 1 (Sham), the abdominal wall was cut without applying torsion and in group 2, ovarian torsion was performed for 2 hours, followed by detorsion for 2 weeks. The hydro-alcoholic extract of orange peel was added to their diet for two weeks in group 3, followed by ovarian torsion for 2 hours and detorsion for 2 hours. Group 4 received the orange peel extract for two weeks and after then ovarian resection for the evaluation of histological damage and blood sampling to examine the serum level of antioxidant enzymes, as well as the expression of PCNA and FSH-R genes in the ovarian tissue. Results: Histological changes in the ovary tissue of rats showed that torsion and detorsion have destructive effects on the ovarian tissue, and torsion/detorsion led to a reduction in the expression of PCNA and FSH-R (P < 0.05). Based on biochemical and hormonal results, the ovarian torsion resulted in an imbalance in the oxidative stress markers and hormone profile of rats. Finally, the administration of the hydroalcoholic extract of orange peel due to its high antioxidant properties improves these effects. Conclusions: In general, administering an appropriate dose of the hydroalcoholic extract of orange peel for two consecutive weeks in the diet had a protective effect on the ovarian tissue at the risk of torsion/detorsion.

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The ameliorative effect of Galega officinalis Extract on Histological Damages, Oxidative Stress Induced by Torsion-Detorsion in Adult Rats’ Ovarian

International Journal of Women s Health and Reproduction Sciences ◽

10.15296/ijwhr.2019.19 ◽

2018 ◽

Vol 7 (1) ◽

pp. 119-123 ◽

Cited By ~ 1

Author(s):

Farnaz Shokri ◽

Majid Shokoohi ◽

Ayda Roudi Rasht Abadi ◽

Hossein Kalarestaghi

Keyword(s):

Oxidative Stress ◽

Ovarian Tissue ◽

Serum Levels ◽

Ovarian Torsion ◽

Oxidative Stress Marker ◽

Blood Levels ◽

Adult Rats ◽

Galega Officinalis ◽

Ameliorative Effect ◽

Tissue Damages

Objectives: The antioxidative role of Galega officinalis extract has been reported in several studies. However, this experimental study was designed in order to investigate the impacts of G. officinalis extract against parameters, such as histological, hormonal, and oxidative stress parameters, which were induced by ovarian torsion/detorsion. Materials and Methods: Adult female Wistar rats (n = 28) were randomly divided into 4 groups including sham (G1), ovarian torsion for 3 hours then-after detorsion (G2 or TD), ovarian torsion-detorsion orally received 50 mg/kg extract of G. officinalis (G3 or TDGO), healthy rats orally received 50 mg/kg hydroalcoholic extract of G. officinalis (G4 or GO). Ten days after torsion-detorsion, rats were sacrificed and their ovaries, and their blood levels of hormones including estrogen and testosterone, as well as some oxidative stress markers were assayed. Results: The structure of ovaries in TD groups of the study showed a notable change compared to other groups. The serum levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH), and also estrogen significantly decreased in TD group, while treatment with G. officinalis could prevent from decreasing mentioned parameters. Furthermore, although torsion-detorsion led to increasing the serum level of malondialdehyde (MDA), it was decreased after administration of G. officinalis. Conclusions: Obtained results showed that G. officinalis could be useful in elevating the estrogen level, reducing the oxidative stress marker (i.e. MDA) and ovarian tissue damages induced by torsion-detorsion.

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Protective Effect of Hydroalcoholic Extract of Solanum surattense on Brain Tissue Damage and Oxidative Stress in Adult Rats with Toxoplasmosis

International Journal of Medical Parasitology and Epidemiology Sciences ◽

10.34172/ijmpes.2020.05 ◽

2020 ◽

Vol 1 (1) ◽

pp. 14-17

Author(s):

Mansour Ataei ◽

Arash Khaki ◽

Yagoob Garedaghi

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Brain Tissue ◽

Tissue Damage ◽

Female Rats ◽

Adult Rats ◽

Hydroalcoholic Extract ◽

Solanum Surattense ◽

Brain Tissue Damage ◽

The Brain

Introduction: Toxoplasmosis is caused by a protozoan named Toxoplasma gondii. This protozoan is a parasite of cats that can spread among other animals and birds around the world and cause a disease that varies from mild to severe. The disease is seen in the forms of acquired toxoplasmosis and congenital toxoplasmosis. Many studies have shown that there is a relationship between reproductive function and toxoplasmosis. T. gondii has led to decreased reproductive performance of males and females in many experimental animals. The aim of this study was to investigate the protective effect of hydroalcoholic extract of Solanum surattense on the brain tissue damage and brain oxidative stress induced by T. gondii in adult rats. Methods: For this purpose, 32 adult female rats were randomly divided into 4 groups. In group 1, 8 healthy rats received IP saline for 3 weeks. In group 2, 8 rats with T. gondii received IP saline for 3 weeks. In group 3, 8 rats with T. gondii received the hydroalcoholic extract of S. surattense for 3 weeks. In group 4, 8 healthy rats received the hydroalcoholic extract of S. surattense for 3 weeks. Then, brain tissue resection was performed to evaluate histological damage and levels of antioxidant enzymes. Results: Histological and biochemical studies showed that T. gondii had a deleterious effect on the brain tissue of rats and increased the level of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ). The administration of hydroalcoholic extract of S. surattense improved these effects due to its high antioxidant properties. Conclusion: The administration of the appropriate dose of hydroalcoholic extract of S. surattense for three consecutive weeks had a protective effect on brain tissue exposed to T. gondii.

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The protective effect of selenium on the lipopolysaccharide-induced oxidative stress and depressed gene expression related to milk protein synthesis in bovine mammary epithelial cells

Biological Trace Element Research ◽

10.1007/s12011-019-01961-7 ◽

2019 ◽

Vol 197 (1) ◽

pp. 141-148 ◽

Cited By ~ 2

Author(s):

Boqi Zhang ◽

Yongmei Guo ◽

Sumei Yan ◽

Xiaoyu Guo ◽

Yanli Zhao ◽

...

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Protein Synthesis ◽

Epithelial Cells ◽

Protective Effect ◽

Milk Protein ◽

Mammary Epithelial Cells ◽

Mammary Epithelial ◽

Bovine Mammary Epithelial Cells

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Reproductive toxicity of inorganic mercury exposure in adult zebrafish: Histological damage, oxidative stress, and alterations of sex hormone and gene expression in the hypothalamic-pituitary-gonadal axis

Aquatic Toxicology ◽

10.1016/j.aquatox.2016.06.018 ◽

2016 ◽

Vol 177 ◽

pp. 417-424 ◽

Cited By ~ 39

Author(s):

Qun-Fang Zhang ◽

Ying-Wen Li ◽

Zhi-Hao Liu ◽

Qi-Liang Chen

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Reproductive Toxicity ◽

Inorganic Mercury ◽

Mercury Exposure ◽

Sex Hormone ◽

Adult Zebrafish ◽

Histological Damage

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The Effect of Modified Porcine Surfactant Alone or in Combination with Polymyxin B on Lung Homeostasis in LPS-Challenged and Mechanically Ventilated Adult Rats

Molecules ◽

10.3390/molecules25194356 ◽

2020 ◽

Vol 25 (19) ◽

pp. 4356 ◽

Cited By ~ 1

Author(s):

Maros Kolomaznik ◽

Jana Kopincova ◽

Zuzana Nova ◽

Juliana Topercerova ◽

Ivan Zila ◽

...

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Lavage Fluid ◽

Polymyxin B ◽

Exogenous Surfactant ◽

Lung Edema ◽

Adult Rats ◽

Mechanically Ventilated ◽

Proinflammatory Mediators ◽

And Oxidative Stress

The study aimed to prove the hypothesis that exogenous surfactant and an antibiotic polymyxin B (PxB) can more effectively reduce lipopolysaccharide (LPS)-induced acute lung injury (ALI) than surfactant treatment alone, and to evaluate the effect of this treatment on the gene expression of surfactant proteins (SPs). Anesthetized rats were intratracheally instilled with different doses of LPS to induce ALI. Animals with LPS 500 μg/kg have been treated with exogenous surfactant (poractant alfa, Curosurf®, 50 mg PL/kg b.w.) or surfactant with PxB 1% w.w. (PSUR + PxB) and mechanically ventilated for 5 hrs. LPS at 500 μg/kg increased lung edema, oxidative stress, and the levels of proinflammatory mediators in lung tissue and bronchoalveolar lavage fluid (BALF). PSUR reduced lung edema and oxidative stress in the lungs and IL-6 in BALF. This effect was further potentiated by PxB added to PSUR. Exogenous surfactant enhanced the gene expression of SP-A, SP-B, and SP-C, however, gene expression for all SPs was reduced after treatment with PSUR + PxB. In mechanically ventilated rats with LPS-induced ALI, the positive effect of exogenous surfactant on inflammation and oxidative stress was potentiated with PxB. Due to the tendency for reduced SPs gene expression after surfactant/PxB treatment topical use of PxB should be considered with caution.

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The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study

International Journal of Reproductive BioMedicine ◽

10.18502/ijrm.v19i3.8575 ◽

2021 ◽

Author(s):

Samira Khani ◽

Maasoume Abdollahi ◽

Azam Khalaj ◽

Hamid Heidari ◽

Somaye Zohali

Keyword(s):

Oxidative Stress ◽

Insulin Resistance ◽

Experimental Study ◽

Polycystic Ovary ◽

Nigella Sativa ◽

Ovarian Tissue ◽

Ovary Syndrome ◽

Hydroalcoholic Extract ◽

Pcos Group ◽

Nigella Sativa Seed

Background: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders among women. Objective: The aim of this study was to investigate the therapeutic effect of hydroalcoholic extract of Nigella sativa (N. sativa) seed as a plant, the consumption of which has been recommended in Islam, on dehydroepiandrosterone (DHEA)-induced PCOS rats. Materials and Methods: This experimental study was carried out on 36 Wistar female rats (3 wk, 60 ± 10 gr). Then rats were divided into 6 groups (n = 6/each): control; PCOSinduced (DHEA 60 mg/kg/sc); PCOS + Metformine (30 mg/kg); and three experimental groups receiving DHEA + hydroalcoholic extract of N. sativa seeds in doses of 50, 100 and 200 mg/kg, respectively. Blood samples were taken for the evaluation of sexual hormones, oxidative stress, glucose, and insulin after 30 days of treatment. Ovarian tissue was used for histopathological study. Results: The serum levels of luteinizing hormone, testosterone, glucose, insulin resistance, malondialdehyde, and insulin (p ≤ 0.001) and estrogen increased while the levels of progesterone (p = 0.01) and antioxidant enzymes in the PCOS group decreased (p ≤ 0.001). Conclusion: The administration of the N. sativa extract to the PCOS rats resulted in remarkable changes in the serumic factors relative to the PCOS group. In addition, the extract improved the structure of the ovarian tissue in the PCOS rat. The histopathological results which are in accordance with biochemical findings imply that N. sativa seed could be useful in the treatment of PCOS, the higher doses of the extract being more effective. Key words: Nigella sativa seed, Oxidative stress, Insulin resistance, Polycystic ovary syndrome, Rat.

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Investigating the Role of Hydroalcoholic Extract of Apium graveolens and Cinnamon zeylanicum on Metabolically Change and Ovarian Oxidative Injury in a Rat Model of Polycystic Ovary Syndrome

International Journal of Women s Health and Reproduction Sciences ◽

10.15296/ijwhr.2019.15 ◽

2018 ◽

Vol 7 (1) ◽

pp. 92-98 ◽

Cited By ~ 2

Author(s):

Fariba Khodaeifar ◽

Seyyed Mohammad Bagher Fazljou ◽

Arash Khaki ◽

Mohammadali Torbati ◽

Elahe Olad Saheb Madarek ◽

...

Keyword(s):

Oxidative Stress ◽

Polycystic Ovary Syndrome ◽

Lipid Profile ◽

Polycystic Ovary ◽

Ovarian Tissue ◽

Apium Graveolens ◽

Ovary Syndrome ◽

Hydroalcoholic Extract ◽

Cinnamon Zeylanicum

Objectives: The present study aimed to compare the role of the hydroalcoholic extract of Apium graveolens and Cinnamon zeylanicum on metabolically change and ovarian oxidative injury in a rat model of polycystic ovary syndrome (PCOS). Materials and Methods: In this experimental research, 64 female Wistar rats with breeding were used including the following groups (G): (I) G1: healthy control; (II) G2: PCOS which received no therapy; (III) G3: PCOS + A. graveolens; (IV) G4: A. graveolens; (V) G5: PCOS + C. zeylanicum; (VI) G6: no PCOS + C. zeylanicum; (VII) G7: PCOS + C. zeylanicum and A. graveolens; and (VIII) G8: C. zeylanicum and A. graveolens. The PCOS was induced by a single dose of the intramuscularly injected estradiol valerate (16 mg/ kg). After 14 days, the animals were anesthetized, then their plasma samples were used to check the blood sugar (BS), insulin, and lipid profile. The ovaries of the rats were removed and fixed for histopathological assessment. In addition, the oxidative stress marker in ovarian tissue was evaluated. Results: The levels of BS, insulin, and lipid profile in plasma significantly enhanced in G2 (P < 0.05) while decreasing significantly in the therapy groups, (P < 0.05). Moreover, a significant decline was observed in the serum level of high-density lipoprotein (HDL) in G2 (P < 0.05) while it enhanced significantly in the therapeutic animals (P < 0.05). Furthermore, a negative change was found in the PCOS group on the ovarian tissue. Besides the oxidative stress enhanced in this tissue while in the treated groups this change was improved. Conclusions: Generally, it was revealed that the extract of A. graveolens and C. zeylanicum had a useful impact on regulating the serum levels of fast blood sugar (FBS), insulin, lipid profile, and oxidative stress markers in the palliation of the PCOS complications.

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Balanced Intense Exercise Training Induces Atrial Oxidative Stress Counterbalanced by the Antioxidant System and Atrial Hypertrophy That Is Not Associated with Pathological Remodeling or Arrhythmogenicity

Antioxidants ◽

10.3390/antiox10030452 ◽

2021 ◽

Vol 10 (3) ◽

pp. 452

Author(s):

Attila Oláh ◽

Bálint András Barta ◽

Alex Ali Sayour ◽

Mihály Ruppert ◽

Eszter Virág-Tulassay ◽

...

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Exercise Training ◽

Right Atrial ◽

Atrial Remodeling ◽

Vigorous Exercise ◽

Adult Rats ◽

Control Male ◽

Increased Risk ◽

Pathological Remodeling

Although regular exercise training is associated with cardiovascular benefits, the increased risk of atrial arrhythmias has been observed after vigorous exercise and has been related to oxidative stress. We aimed at investigating exercise-induced atrial remodeling in a rat model of an athlete’s heart and determining sex-specific differences. Age-matched young adult rats were divided into female exercised, female control, male exercised, and male control groups. After exercised animals completed a 12-week-long swim training protocol, echocardiography and in vivo cardiac electrophysiologic investigation were performed. Additionally, atrial histological and gene expression analyses were carried out. Post-mortem atrial weight data and histological examination confirmed marked atrial hypertrophy. We found increased atrial gene expression of antioxidant enzymes along with increased nitro-oxidative stress. No gene expression alteration was found regarding markers of pathological remodeling, apoptotic, proinflammatoric, and profibrotic processes. Exercise training was associated with a prolonged right atrial effective refractory period. We could not induce arrhythmias by programmed stimulation in any groups. We found decreased expression of potassium channels. Female gender was associated with lower profibrotic expression and collagen density. Long-term, balanced exercise training-induced atrial hypertrophy is not associated with harmful electrical remodeling, and no inflammatory or profibrotic response was observed in the atrium of exercised rats.

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