Expression patterns of five heat shock proteins in Sesamia inferens (Lepidoptera: Noctuidae) during heat stress

2015 ◽  
Vol 18 (3) ◽  
pp. 529-533 ◽  
Author(s):  
Xiao-Tian Tang ◽  
Meng Sun ◽  
Ming-Xing Lu ◽  
Yu-Zhou Du
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Expression Patterns ◽  
Sesamia Inferens ◽  
Shock Proteins ◽  
Lepidoptera Noctuidae

scholarly journals 607 Modulation of Heat Shock Proteins in Potato Leaves by Rhizospheric Calcium: Mitigation of Heat Stress Effect

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 501E-502
Author(s):  
Sookhee Park ◽  
Jiwan P. Palta
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Calcium Concentration ◽  
Expression Patterns ◽  
Dry Weight ◽  
Potato Production ◽  
Stress Effect ◽  
Shock Proteins

High temperature effects potato production by reducing overall growth and partitioning of photosynthate to tubers. Recent studies from our laboratory demonstrated that these effects can be reduced by increasing rhizospheric calcium. This present study was conducted to determine if this mitigation of heat stress effect on potato is due to modulation of heat shock protein by calcium during stress. An inert medium and nutrient delivery system capable of maintaining precise rhizospheric calcium levels were used. Biomass was measured and protein samples were collected from potato leaves. Using electroblotting, heat shock proteins were detected by antibodies to Hsp21 and Hsp70 (obtained from Dr. Elizabeth Vierling). Injury by prolonged heat stress was mitigated at calcium concentration >5 ppm. The calcium concentration of leaf and stem tissues were twice as high in 25 ppm calcium-treated plant compared to 1 ppm calcium-treated plants. Total foliage fresh weight was 33% higher and dry weight 20% higher in plants supplied with 25 ppm of calcium than supplied with 1 ppm of calcium. HSP21 was expressed only at high temperature and at greater concentrations in 25 ppm calcium treatment. HSP70 was expressed in both control, 20 °C/15 °C (day/night) and heat-stressed tissue, 35 °C/25 °C (day/night) under various calcium treatments (1 to 25 ppm). Also, there were some differences in HSPs expression patterns between young and mature leaves. Young tissue responded immediately to the heat stress and started to express HSP21 within 1 day. Mature tissue started to express HSP21 after 2 days. HSP21 of young tissue disappeared sooner than mature tissue when heat stress-treated plants were returned to normal conditions. These results support our earlier studies indicating that an increase in rhizospheric calcium mitigate heat stress effects on the potato plant. Furthermore these results suggest that this mitigation may be due to modulation of HSP21by rhizospheric calcium during heat stress.

scholarly journals Proteomic analysis of heat shock proteins in maize (Zea mays L.)

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Mahmoud Hussien Abou-Deif ◽  
Mohamed Abdel-Salam Rashed ◽  
Kamal Mohamed Khalil ◽  
Fatma El-Sayed Mahmoud
Keyword(s):  
Heat Treatment ◽  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Proteome Analysis ◽  
High Temperature Stress ◽  
Heat Treatments ◽  
Maize Plants ◽  
Adverse Influence ◽  
Shock Proteins

Abstract Background Maize is one of the important cereal food crops in the world. High temperature stress causes adverse influence on plant growth. When plants are exposed to high temperatures, they produce heat shock proteins (HSPs), which may impart a generalized role in tolerance to heat stress. Proteome analysis was performed in plant to assess the changes in protein types and their expression levels under abiotic stress. The purpose of the study is to explore which proteins are involved in the response of the maize plant to heat shock treatment. Results We investigated the responses of abundant proteins of maize leaves, in an Egyptian inbred line of maize “K1”, upon heat stress through two-dimensional electrophoresis (2-DE) on samples of maize leaf proteome. 2-DE technique was used to recognize heat-responsive protein spots using Coomassie Brilliant Blue (CBB) and silver staining. In 2-D analysis of proteins from plants treated at 45 °C for 2 h, the results manifested 59 protein spots (4.3%) which were reproducibly detected as new spots where did not present in the control. In 2D for treated plants for 4 h, 104 protein spots (7.7%) were expressed only under heat stress. Quantification of spot intensities derived from heat treatment showed that twenty protein spots revealed clear differences between the control and the two heat treatments. Nine spots appeared with more intensity after heat treatments than the control, while four spots appeared only after heat treatments. Five spots were clearly induced after heat treatment either at 2 h or 4 h and were chosen for more analysis by LC-MSMS. They were identified as ATPase beta subunit, HSP26, HSP16.9, and unknown HSP/Chaperonin. Conclusion The results revealed that the expressive level of the four heat shock proteins that were detected in this study plays important roles to avoid heat stress in maize plants.

scholarly journals Proteome and Transcriptome Reveal Involvement of Heat Shock Proteins and Indoleacetic Acid Metabolism Process in Lentinula Edodes Thermotolerance

2018 ◽  
Vol 50 (5) ◽  
pp. 1617-1637 ◽  
Author(s):  
Gang-Zheng Wang ◽  
Chao-Jun Ma ◽  
Yi Luo ◽  
Sha-Sha Zhou ◽  
Yan Zhou ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Indoleacetic Acid ◽  
Lentinula Edodes ◽  
Acid Biosynthesis ◽  
Over Expression ◽  
Indoleacetic Acid Biosynthesis ◽  
Shock Proteins ◽  
Exogenous Iaa

Background/Aims: Heat stress could cause huge losses for Lentinula edodes in China and other Asian cultivation areas. Yet our understanding of mechanism how to defend to heat stress is incomplete. Methods: Using heat-tolerant and heat-sensitive strains of L. edodes, we reported a combined proteome and transcriptome analysis of L. edodes response to 40 °C heat stress for 24 h. Meanwhile, the effect of LeDnaJ on the thermotolerance and IAA (indoleacetic acid) biosynthesis in L. edodes was analyzed via the over-expression method. Results: The proteome results revealed that HSPs (heat shock proteins) such as Hsp40 (DnaJ), Hsp70, Hsp90 and key enzymes involved in tryptophan and IAA metabolism process LeTrpE, LeTrpD, LeTam-1, LeYUCCA were more highly expressed in S606 than in YS3357, demonstrating that HSPs and tryptophan as well as IAA metabolism pathway should play an important role in thermotolerance. Over-expression of LeDnaJ gene in S606 strains showed better tolerance to heat stress. It was also documented that intracellular IAA accumulation of S606 (8-fold up) was more than YS3357 (2-fold up), and exogenous IAA enhanced L. edodes tolerance to heat stress. Conclusion: Our data support the interest of LeTrpE, LeDnaJ, tryptophan and IAA could play a pivotal role in enhancing organism thermotolerance.

scholarly journals Immunomodulation of function of small heat shock proteins prevents their assembly into heat stress granules and results in cell death at sublethal temperatures

2004 ◽  
Vol 41 (2) ◽  
pp. 269-281 ◽  
Author(s):  
Sergey Miroshnichenko ◽  
Joanna Tripp ◽  
Uta zur Nieden ◽  
Dieter Neumann ◽  
Udo Conrad ◽  
...  
Keyword(s):  
Cell Death ◽  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Stress Granules ◽  
Small Heat Shock Proteins ◽  
Shock Proteins
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