Biotransformation of 5-hydroxymethylfurfural and furfural with bacteria of bacillus genus

2022 ◽  
pp. 102281
Author(s):  
Mónica L. Becerra ◽  
Luz M. Lizarazo ◽  
Hugo A. Rojas ◽  
Gloria A. Prieto ◽  
José J. Martinez
Keyword(s):  
Bacillus Genus

PRODUCTION OF CYTASES ACTIVE ON BARLEY GUM BY BACTERIA OF THE GENUS BACILLUS

1953 ◽  
Vol 31 (1) ◽  
pp. 28-32 ◽  
Author(s):  
A. C. Blackwood
Keyword(s):  
Bacillus Subtilis ◽  
Enzymatic Activity ◽  
Nitrogen Source ◽  
Freeze Drying ◽  
Shake Flask ◽  
Sole Nitrogen Source ◽  
Genus Bacillus ◽  
Original Activity ◽  
Bacterial Cultures ◽  
Bacillus Genus

One hundred and fourteen bacterial cultures representing most of the species in the Bacillus genus were tested for the production of extracellular barley gum cytase. Assays were made on shake-flask cultures grown on a medium containing glucose and yeast extract. Although all the organisms had some enzymatic activity, certain strains of Bacillus subtilis gave the best yields of cytase. On a medium with asparagine as the sole nitrogen source even higher yields were obtained. The crude cytase preparations were stable and after freeze-drying most of the original activity remained.

scholarly journals Exometabolites of endospore-forming bacteria of Bacillus genus identified by genomic-metabolomic profiling

2020 ◽  
Vol 92 (6) ◽  
pp. 154-164
Author(s):  
А. M. Ostapchuk ◽  
◽  
М. D. Shtenikov ◽  
V. О. Ivanytsia ◽  
Keyword(s):  
Metabolomic Profiling ◽  
Bacillus Genus

scholarly journals The Inhibitory Effect of Lactobacillus rhamnosus on Bacillus Genus as Rope Disease Agent, in Flour Medium

2015 ◽  
Vol 43 (4) ◽  
pp. 155-164
Author(s):  
Selcuk ARSLAN ◽  
Zerrin ERGINKAYA ◽  
Mehmet OZASLAN ◽  
I. Halil KILIC ◽  
Emel UNAL
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Inhibitory Effect ◽  
Disease Agent ◽  
Bacillus Genus

Evaluation of the presence of Bacillus genus microorganisms in honey

2018 ◽  
Vol 280 ◽  
pp. S52
Author(s):  
Nicoleta Ciocirlie ◽  
Laurentiu Tudor ◽  
Elena Mitranescu
Keyword(s):  
Bacillus Genus

scholarly journals Development of a probiotic for animals and aquaculture based on Bacillus toyonensis B-13249 and Bacillus pumilus B-13250 strains

2021 ◽  
Vol 11 (3) ◽  
pp. 393-402
Author(s):  
A. V. Malkova ◽  
I. Yu. Evdokimov ◽  
M. V. Shirmanov ◽  
A. N. Irkitova ◽  
D. E. Dudnik
Keyword(s):  
No Value ◽  
Nutrient Medium ◽  
Bacillus Pumilus ◽  
Ph Value ◽  
Expiry Date ◽  
Maximum Value ◽  
Bacillus Genus ◽  
Number Of Bacteria ◽  
Bacillus Toyonensis ◽  
Selection Of

Abstract: This article aims to develop a probiotic for animals and aquaculture based on the Bacillus toyonensis B-13249 and Bacillus pumilus B-13250 strains. The selection of a nutrient medium was conducted for cultivating the inoculum of these microorganisms. Several bacteria fermentations of the Bacillus genus were performed in biological reactors with a capacity of 15 and 250 l. A technology for obtaining a finished probiotic for animals and aquaculture was developed. The results indicate that L-broth is the most optimal nutrient medium for cultivating the studied strains. The cultivation of B. toyonensis B-13249 and B. pumilus B-13250 strains in fermenters revealed that sporulation begins after 4–8 hours of fermentation. In contrast to the vegetative medium, the fermentative medium helped the bacilli develop a higher optical density (the maximum value in the B. pumilus strain – 2.400±0.149), pH value (maximum value in the B. toyonensis strain – 8.483±0.609) and titer (at least 1010 CFU/g). After 20–24 hours of incubation, both strains of bacilli in the fermenter, almost completely pass into endospores, which serve as a signal for the start of biomass centrifugation. This was indicated by the following: from a 15 l fermenter – 83.3±6.1 g of concentrate, from a 250 l fermenter – 499.8±51.4 g. The number of bacilli in a concentrated state was at least 1·1011 CFU/g for both strains. Obtaining a finished preparation required mixing bacterial concentrates with maltodextrin to a titer of at least 1·1010 CFU/g. The number of bacteria in the preparation checked every month during the year, recorded no value less than 1·1010 CFU/g. Thus, L-broth is most favorable for growing the mother culture of the B. toyonensis B-13249 and B. pumilus B-13250 strains, and fermentative nutrient medium – for the cultivation in fermenters. The expiry date of the bacilli-based biological preparation is at least 12 months, during which the drug’s polycomponence, color and consistency are preserved, in addition to the bacteria titer (at least 1·1010 CFU/g) and their viability.

scholarly journals Antimicrobial activity of bacteria isolated from tissue of the coral Palythoa caribaeorum (Zoantharia: Sphenopidae) from Paraíba, Brazil coastal reefs

2021 ◽  
Vol 69 (2) ◽  
Author(s):  
Jalcinês C. Pereira ◽  
Krystyna Gorlach-Lira ◽  
Bruno O. de Veras
Keyword(s):  
Antimicrobial Activity ◽  
Culture Cell ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Associated Bacteria ◽  
Microdilution Method ◽  
Palythoa Caribaeorum ◽  
Bacillus Genus ◽  
Low Cytotoxicity

Introduction: The coral-associated bacteria with antimicrobial activity may be important to promote the health of their host through various interactions, and may be explored as a source of new bioactive compounds. Objective: To analyze the antimicrobial activity of bacteria associated with the zoanthid Palythoa caribaeorum from the coral reefs of Carapibus, Paraiba state, Brazil. Methods: The phylogenetic analysis of the bacteria was conducted based on partial sequences of the 16S rRNA gene using molecular and bioinformatics tools. The antimicrobial activity of the 49 isolates was tested against four bacterial strains and one yeast strain: Bacillus cereus (CCT0198), Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa and Candida albicans (ATCC 10231). The antibiosis and antibiogram assays were conducted and the Minimal Inhibitory Concentration (MIC) was determined by the microdilution method. Results: The bacterial isolates belonged to Firmicutes phylum (84 % of the isolates) and the Proteobacteria phylum (16 % of the isolates). Among the 49 isolates five genera were found, with the Bacillus genus being the most abundant (82 % of the isolates), followed by Vibrio (10 %), Pseudomonas (4 %), Staphylococcus (2 %) and Alteromonas (2 %). Antibiosis test revealed that 16 isolates (33 %) showed antimicrobial activity against one or more of five tested reference strains. The highest number of antagonistic bacteria were found in the Bacillus genus (12 isolates), followed by Vibrio (three isolates) and Pseudomonas (one isolate) genera. The B. subtilis NC8 was the only isolate that inhibited all tested strains in the antibiosis assay. However, antibiogram test with post-culture cell-free supernatant of NC8 isolate showed the inhibition of only B. cereus, S. aureus and C. albicans, and the lyophilized and dialyzed material of this isolate inhibited only B. cereus. The lyophilized material showed bacteriostatic activity against B. cereus, with a MIC value of 125 μg/μl, and in the cytotoxicity assay, the hemolysis value was of 4.8 %, indicating its low cytotoxicity. Conclusions: The results show the antimicrobial potential of some bacterial isolates associated with the P. caribaeourum tissue, especially those belonged to Bacillus genus.

scholarly journals Features of isolation of the anthrax pathogen depending on the type of nutrient medium

2020 ◽  
Vol 11 (3) ◽  
pp. 4318-4322
Author(s):  
Nariman S. Sadykov ◽  
Ramzi N. Nizamov ◽  
Elmira N. Mustafina ◽  
Marina Yu. Gallyamova ◽  
Timur R. Mustafin ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
External Environment ◽  
Soil Samples ◽  
Complete Dissolution ◽  
Nutrient Media ◽  
Bacillus Genus ◽  
Positive Evaluation

The purpose of the work is to develop a nutrient medium for differentiation of bacillus from soil aerobic bacilli. In order to achieve the set goal, we used the method of introduction into the environment of cultivation of microorganisms separated from animals and objects of external environment (water, soil, feed, air, scrapes from different surfaces suspected of contamination by their bacillus ) of nutrient substrate sucrose, used by bacteria of Bacillus genus for synthesis of product of their metabolism, a sign absent in bacillus . This feature is essential for identification and differentiation of bacillus from closely related saprophytes. To identify and differentiate the bacillus , the microbes isolated from the external environment were cultivated in a nutrient medium consisting of agar (MPA) and () synthesis of sucrose in the amount of 10% to 100 ml of melted agar. The proposed nutrient media was prepared as follows. agar (500 ml) was melted at 1000C, 10 g of sucrose was added per 100 ml of medium and after the complete dissolution of sucrose, the nutrient medium was poured into dishes and used for sowing the studied material for identification and differentiation of grown crops. The efficiency of the method has been tested in production experiments with positive evaluation. For this purpose, soil samples taken from the territory of old cattle cemeteries were fractionally sown on MPA and for 16-18 hours at 370C and examined crops for the presence of matte and rough (R-form) colonies.

scholarly journals MICROBIAL SUCCESSIONS AT CHICKEN MANURE COMPOSTING WITH CELLULOLYTIC MICROORGANISMS

2017 ◽  
Vol 26 ◽  
pp. 24-29
Author(s):  
M. V. Miahka ◽  
S. M. Derkach ◽  
V. V. Volkohon ◽  
S. B. Dimova ◽  
O. V. Pyrih ◽  
...  
Keyword(s):  
Organic Substrate ◽  
Chicken Manure ◽  
Trophic Groups ◽  
Cellulolytic Microorganisms ◽  
Bacillus Genus ◽  
Positive Effect

Chicken manure composting with selected cellulosolytic microorganisms provides differenteffects on the development of microorganisms of certain ecological trophic groups. Introduction tothe organic substrate bacteria of the Bacillus genus promoted the increase of the cellulolytic bacteriaquantity, while addition of the bacteria of Trichoderma genus stabilized the number of micromycetes in the compost. Microorganisms that have the greatest positive effect on the developmentof compost microbiota were selected

scholarly journals The effect of wheat bread contamination by the Bacillus genus bacteria on the quality and safety of bread

2015 ◽  
Vol 102 (3) ◽  
pp. 351-358 ◽  
Author(s):  
Lina Vaičiulytė-Funk ◽  
Renata Žvirdauskienė ◽  
Joana Šalomskienė ◽  
Antanas Šarkinas
Keyword(s):  
Wheat Bread ◽  
Quality And Safety ◽  
Bacillus Genus
Sign in / Sign up

Export Citation Format

Share Document