scholarly journals The Inhibitory Effect of Lactobacillus rhamnosus on Bacillus Genus as Rope Disease Agent, in Flour Medium

2015 ◽  
Vol 43 (4) ◽  
pp. 155-164
Author(s):  
Selcuk ARSLAN ◽  
Zerrin ERGINKAYA ◽  
Mehmet OZASLAN ◽  
I. Halil KILIC ◽  
Emel UNAL
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Inhibitory Effect ◽  
Disease Agent ◽  
Bacillus Genus

scholarly journals Good Bugs vs Bad Bugs: Evaluation of Inhibitory Effect of Selected Probiotics against Enterococcus faecalis

2017 ◽  
Vol 18 (4) ◽  
pp. 312-316 ◽  
Author(s):  
Aarti A Bohora ◽  
Sharad R Kokate
Keyword(s):  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Pathogenic Bacteria ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Indicator Strain ◽  
Oral Bacteria ◽  
Diffusion Method

ABSTRACT Introduction The main goal of endodontics is the prevention of apical periodontitis. This is due to the presence of persistent pathogenic microorganisms, such as Enterococcus faecalis, and its ability to directly cause acute and chronic inflammation in the periapical tissues. Lactobacillus has been shown to promote health in the intestines as well as to inhibit the growth of certain problematic oral bacteria. This study explores shifting the established paradigm of endodontic treatment, which has focused on eliminating all bacteria from the canal system and on elimination of the problematic bacteria through introducing probiotics. A preliminary work was performed to evaluate the possible effectiveness of probiotics in preventing the growth of E. faecalis. Materials and methods Two probiotic cultures Lactobacillus plantarum ATCC 8041 and Lactobacillus rhamnosus ATCC 7408 were selected to check their antimicrobial activity against E. faecalis ATCC 29212 by two methods: agar cup/well diffusion method and deferred antagonism test. Agar cup method A total of 0.5 mL of requisite test pathogen culture was inoculated into 20 mL of molten sterile Mueller and Hinton agar and cooled to 45 ± 2°C. Circular wells of diameter 10 mm were punched in each of the poured plates. Appropriately diluted test samples were added to the above-punched wells. The plates were incubated upright position at 37°C for 24 hours in aerobic conditions. Postincubation, zone of inhibition was measured. The cell-free supernatant of Lactobacillus species was also evaluated for antimicrobial activity. Deferred antagonism test The test probiotic strain was standardized to 0.1 optical density (OD) at 600 nm and inoculated in a 1 cm wide diametric streak across the surface of trypticase soy agar + yeast extract + calcium carbonate (TSYCa) agar using a sterile cotton swab. Then, the plate agar containing the test strain was incubated at 37°C for 24 hours under aerophilic condition and then standardized to 0.1 OD at 600 nm for overnight (18 hours, 37°C). A purified culture of indicator strain (pathogen) was streaked at right angles to the line of original producer growth. Postincubation plates were observed for the inhibition zone width of the indicator strain. Results Under the conditions of this study, Lactobacilli had an inhibitory effect on the growth of E. faecalis by agar cup method but not by deferred antagonism test. Conclusion This pilot study demonstrated that probiotics show a potential in root canal therapy. Clinical Significance If probioitcs are effective against endodontic pathogens, they can be potentially used as intracanal medicaments. This will be a novel concept of introducing bacteriotherapy in endodontics and replacing pathogenic bacteria by healthy bacteria, normal flora. How to cite this article Bohora AA, Kokate SR. Good Bugs vs Bad Bugs: Evaluation of Inhibitory Effect of Selected Probiotics against Enterococcus faecalis. J Contemp Dent Pract 2017;18(4):312-316.

Inhibitory activity in vitro of probiotic lactobacilli against oral Candida under different fermentation conditions

2015 ◽  
Vol 6 (3) ◽  
pp. 361-368 ◽  
Author(s):  
Q. Jiang ◽  
I. Stamatova ◽  
K. Kari ◽  
J.H. Meurman
Keyword(s):  
Inhibitory Activity ◽  
Candida Species ◽  
Lactobacillus Reuteri ◽  
Lactobacillus Rhamnosus ◽  
Inhibitory Effect ◽  
Lactobacillus Bulgaricus ◽  
Ph Values ◽  
Probiotic Lactobacilli ◽  
Oral Candida

Clinical studies have shown that probiotics positively affect oral health by decreasing gum bleeding and/or reducing salivary counts of certain oral pathogens. Our aim was to investigate the inhibitory effect of six probiotic lactobacilli against opportunistic oral Candida species. Sugar utilisation by both lactobacilli and Candida was also assessed. Agar overlay assay was utilised to study growth inhibition of Candida albicans, Candida glabrata and Candida krusei by Lactobacillus rhamnosus GG, Lactobacillus casei Shirota, Lactobacillus reuteri SD2112, Lactobacillus brevis CD2, Lactobacillus bulgaricus LB86 and L. bulgaricus LB Lact. The inhibitory effect was measured at pH 5.5, 6.4, and 7.2, respectively, and in the presence of five different carbohydrates in growth medium (glucose, fructose, lactose, sucrose, and sorbitol). Growth and final pH values were measured at two-hour time points to 24 h. L. rhamnosus GG showed the strongest inhibitory activity in fructose and glucose medium against C. albicans, followed by L. casei Shirota, L. reuteri SD2112 and L. brevis CD2. None of the lactobacilli tested affected the growth of C. krusei. Only L. rhamnosus GG produced slight inhibitory effect on C. glabrata. The lower pH values led to larger inhibition zones. Sugar fermentation profiles varied between the strains. L. casei Shirota grew in the presence of all sugars tested, whereas L. brevis CD2 could utilise only glucose and fructose. All Candida species metabolised the available sugars but the most rapid growth was observed with C. glabrata. The results suggest that commercially available probiotics differ in their inhibitory activity and carbohydrate utilisation; the above properties are modified by different pH values and sugars with more pronounced inhibition at lower pH.

Intestinal flora imbalance affects the prognosis of Parkinson's disease by regulating the expression of fecal microRNA-146a and GDNF: A Preliminary Study

2021 ◽  
Author(s):  
Hongchun Wei ◽  
Yinbao Hu ◽  
Liqing Yang
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Intestinal Flora ◽  
Lactobacillus Rhamnosus ◽  
Inhibitory Effect ◽  
Luciferase Assay ◽  
Regulatory Relationship ◽  
Tissue Samples ◽  
Protein Levels

IntroductionThere is mounting evidence showing the association between the gut microbiome and Parkinson’s disease (PD). In this study, we aimed to explore the role of intestinal flora imbalance in the progression of PD and the underlying molecular mechanism involving the regulatory relationship between miR-146a and glial cell line–derived neurotrophic factor (GDNF).Material and methodsA total of 42 PD patients were recruited in this study and grouped according to their prognosis. Real-time PCR, computational analysis and luciferase assay were carried out to explore the role of miR-146a and GDNF in PD patients as well as the regulatory relationship between the expression of miR-146a and GDNF. A rat model was used to study the effect of intestinal flora imbalance by treating the rats with Lactobacillus rhamnosus NCDC17.ResultsA lower count of total lactobaccilus and B. fragilis may predict the progression of PD. GDNF was identified as a virtual target gene of miR-146a, whose expression in fecal, plasma and cerebral tissue samples was significantly reduced in PD patients. In addition, the presence of miR-146a precursors and GDNF siRNA significantly reduced the mRNA and protein levels of GDNF in SH-SY5Y and U251 cells, and the inhibitory effect of miR-146a precursors on GDNF expression was similar to that of GDNF siRNA.ConclusionsTaken together, the findings of this study demonstrated that GDNF is a key factor involved in the prognosis of PD. we suggested that the administration of Lactobacillus rhamnosus NCDC17 promoted the pathogenesis of PD by regulating the expression of miR-146a and GDNF in the brain.

scholarly journals Survival of Lactobacillus rhamnosus EM1107 in simulated gastrointestinal conditions and its inhibitory effect against pathogenic bacteria in semi-hard goat cheese

LWT ◽  
2015 ◽  
Vol 63 (2) ◽  
pp. 807-813 ◽  
Author(s):  
Fernanda Rodrigues Leite Rolim ◽  
Karina Maria Olbrich dos Santos ◽  
Samuel Carneiro de Barcelos ◽  
Antônio Sílvio do Egito ◽  
Thais Santana Ribeiro ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Lactobacillus Rhamnosus ◽  
Inhibitory Effect ◽  
Goat Cheese ◽  
Gastrointestinal Conditions

scholarly journals Inhibitory effect of essential oils against Lactobacillus rhamnosus and starter culture in fermented milk during its shelf-life period

2012 ◽  
Vol 43 (3) ◽  
pp. 1147-1156 ◽  
Author(s):  
Cristiane Mengue Feniman Moritz ◽  
Vera Lúcia Mores Rall ◽  
Margarida Júri Saeki ◽  
Ary Fernandes Júnior
Keyword(s):  
Essential Oils ◽  
Shelf Life ◽  
Starter Culture ◽  
Lactobacillus Rhamnosus ◽  
Fermented Milk ◽  
Inhibitory Effect ◽  
Life Period

scholarly journals Lactobacillus rhamnosusGG Suppresses MeningiticE. coliK1 Penetration across Human Intestinal Epithelial Cells In Vitro and Protects Neonatal Rats against Experimental Hematogenous Meningitis

2009 ◽  
Vol 2009 ◽  
pp. 1-7 ◽  
Author(s):  
Sheng-He Huang ◽  
Lina He ◽  
Yanhong Zhou ◽  
Chun-Hua Wu ◽  
Ambrose Jong
Keyword(s):  
Neonatal Sepsis ◽  
Lactobacillus Rhamnosus ◽  
Inhibitory Effect ◽  
Neonatal Rat ◽  
Control Group ◽  
Dependent Manner ◽  
Neonatal Rats ◽  
E Coli

The purpose of this study was to examine prophylactic efficacy of probiotics in neonatal sepsis and meningitis caused byE. coliK1. The potential inhibitory effect ofLactobacillus rhamnosusGG (LGG) on meningiticE. coliK1 infection was examined by using (i) in vitro inhibition assays with E44 (a CSF isolate from a newborn baby withE. colimeningitis), and (ii) the neonatal rat model ofE. colisepsis and meningitis. The in vitro studies demonstrated that LGG blocked E44 adhesion, invasion, and transcytosis in a dose-dependent manner. A significant reduction in the levels of pathogen colonization,E. colibacteremia, and meningitis was observed in the LGG-treated neonatal rats, as assessed by viable cultures, compared to the levels in the control group. In conclusion, probiotic LGG strongly suppresses meningiticE. colipathogens in vitro and in vivo. The results support the use of probiotic strains such as LGG for prophylaxis of neonatal sepsis and meningitis.

scholarly journals A Promising Probiotic Irrigant: An In Vitro Study

2019 ◽  
Vol 7 (3) ◽  
pp. 407-411
Author(s):  
Hoda El-sayed ◽  
Yousra Aly ◽  
Hanaa Elgamily ◽  
Mohamed M. Nagy
Keyword(s):  
Enterococcus Faecalis ◽  
Lactobacillus Rhamnosus ◽  
In Vitro Study ◽  
Inhibitory Effect ◽  
Mean Value ◽  
Negative Control ◽  
Control Group ◽  
Anterior Teeth ◽  
Group 3

AIM: The present study aimed to investigate the inhibitory effect of Lactobacillus rhamnosus (B-445) as a probiotics irrigant on the growth of Enterococcus faecalis. METHODS: Forty-two extracted single human canal anterior teeth were prepared with rotary instrumentation and sterilised. Teeth were divided into 3 groups according to the type of irrigant, N = 14. Three experimental groups were inoculated with E. faecalis and cultured for 21 days before use; Group 1 was 2.5% NaOCl (positive control), Group 2 was saline (negative control), Group 3 was the experimental probiotic irrigant. Paper point sampling of the canals of each group was obtained before irrigation (S1), immediately after irrigation (S2) and after 24 hours (post irrigation samples) (S3) to determine remaining colony forming units for E. faecalis. Also, Colony counts for L. rhamnosus in Group 3 after immediate irrigation, as well as 24 hours post irrigation, was performed to determine the survival profile of these bacteria in infected root canal with E. faecalis. RESULTS: The NaOCl irrigant group had the lowest mean value of (log 10 CFU/mL) of E. faecalis after immediate irrigation and after 24 hrs post irrigation followed by the probiotic group, while the highest mean value was the saline group (P ≤ 0.001). The survival profile for L. rhamnosus in Group 3 after immediate irrigation and post-irrigation were slightly higher than for E. faecalis (P ≤ 0.001). CONCLUSION: Lactobacillus rhamnosus which revealed a potential inhibitory effect on the growth of Enterococcus faecalis, could be used as a new natural, safe probiotic irrigant agent. h of Enterococcus faecalis, could be used as a new natural, safe probiotic irrigant agent.

scholarly journals Antifungal and Antivirulence Activity of Vaginal Lactobacillus Spp. Products against Candida Vaginal Isolates

Pathogens ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 150 ◽  
Author(s):  
Camilla Itapary dos Santos ◽  
Yasmine Ramos França ◽  
Carmem Duarte Lima Campos ◽  
Maria Rosa Quaresma Bomfim ◽  
Bruna Oliveira Melo ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Lactobacillus Rhamnosus ◽  
Inhibitory Effect ◽  
Antagonistic Activity ◽  
Antibiofilm Activity ◽  
Vaginal Lactobacillus ◽  
Best Value ◽  
Reducing Activity ◽  
Lactobacillus Spp

Candida yeasts are generally found in the vaginal microbiota; however, disruption of the balance maintained by host factors and microorganisms results in vulvovaginal candidiasis (VVC). This study evaluated the antagonistic activity of vaginal Lactobacillus spp. on Candida albicans to verify whether active compounds of Lactobacillus spp. had antifungal and antivirulence activity. The antagonism assay showed that 15 out of 20 Lactobacillus strains had an inhibitory effect on C. albicans. Biosurfactants displayed surface-tension-reducing activity, with the best value obtained for Lactobacillus gasseri 1. Lactobacillus rhamnosus ATCC 9595, Lactobacillus acidophilus ATCC 4356, and Lactobacillus paracasei 11 produced biosurfactants that decreased C. albicans adhesion and disrupted biofilm formation. The best results were obtained in the pre-incubation assay for L. gasseri 1 and L. paracasei 11. Overall, Lactobacillus strains showed significant anti-Candida activity, and their biosurfactants exhibited considerable anti-adhesion and antibiofilm activity against C. albicans. To be considered safe for use in vivo, the safety of biosurfactant (BS) should be investigated using cytotoxicity assays.

Inhibitory effect of melatonin on nitric oxide synthase in rat enteric nervous system

2001 ◽  
Vol 120 (5) ◽  
pp. A176-A176
Author(s):  
P KOPPITZ ◽  
M STORR ◽  
D SAUR ◽  
M KURJAK ◽  
H ALLESCHER
Keyword(s):  
Nitric Oxide ◽  
Nervous System ◽  
Nitric Oxide Synthase ◽  
Enteric Nervous System ◽  
Inhibitory Effect ◽  
Oxide Synthase
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