Neuroprotectin D1, a lipid anti-inflammatory mediator, in patients with intracerebral hemorrhage

Abstract Background Neuroinflammation is important in the pathophysiology of spontaneous intracerebral hemorrhage (ICH) and peripheral inflammatory cells play a role in the clinical evolution and outcome. Methodology Blood samples from ICH patients (n = 20) were collected at admission for 5 consecutive days for peripheral blood mononuclear cells (PBMCs). Frozen PBMCs were used for real-time PCR using Taqman probes (NFKB1, SOD1, PPARG, IL10, NFE2L2, and REL) and normalized to GAPDH. Data on hospital length of stay and modified Rankin score (MRS) were collected with 90-day MRS ≤ 3 as favorable outcome. Statistical analysis of clinical characteristics to temporal gene expression from early to delayed timepoints was compared for MRS groups (favorable vs unfavorable) and hematoma volume. Principle findings and results IL10, SOD1, and REL expression were significantly higher at delayed timepoints in PBMCs of ICH patients with favorable outcome. PPARG and REL increased between timepoints in patients with favorable outcome. NFKB1 expression was not sustained, but significantly decreased from higher levels at early onset in patients with unfavorable outcome. IL10 expression showed a negative correlation in patients with high hematoma volume (>30 mL). Conclusions and significance Anti-inflammatory, pro-survival regulators were highly expressed at delayed time points in ICH patients with a favorable outcome, and IL10 expression showed a negative correlation to high hematoma volume.

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Preeclampsia is associated with a deficiency of lipoxin A4, an endogenous anti-inflammatory mediator

Fertility and Sterility ◽

10.1016/j.fertnstert.2014.03.056 ◽

2014 ◽

Vol 102 (1) ◽

pp. 282-290.e4 ◽

Cited By ~ 30

Author(s):

Zhangye Xu ◽

Feng Zhao ◽

Feng Lin ◽

Huiqiu Xiang ◽

Ni Wang ◽

...

Keyword(s):

Inflammatory Mediator ◽

Lipoxin A4 ◽

Anti Inflammatory

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Accumulation of Non-steroidal Anti-inflammatory Drugs by Gingival Fibroblasts

Journal of Dental Research ◽

10.1177/154405910608500511 ◽

2006 ◽

Vol 85 (5) ◽

pp. 452-456 ◽

Cited By ~ 1

Author(s):

M.M. Zavarella ◽

O. Gbemi ◽

J.D. Walters

Keyword(s):

Steady State ◽

Connective Tissue ◽

Inflammatory Mediator ◽

Dependent Manner ◽

Gingival Fibroblasts ◽

Temperature Dependent ◽

Tnf Α ◽

Steady State Levels ◽

Inflammatory Drugs ◽

Anti Inflammatory

Non-steroidal anti-inflammatory drugs (NSAIDs) are used to manage pain and inflammatory disorders. We hypothesized that gingival fibroblasts actively accumulate NSAIDs and enhance their levels in gingival connective tissue. Using fluorescence to monitor NSAID transport, we demonstrated that cultured gingival fibroblasts transport naproxen in a saturable, temperature-dependent manner with a Km of 127 μg/mL and a Vmax of 1.42 ng/min/μg protein. At steady state, the intracellular/extracellular concentration ratio was 1.9 for naproxen and 7.2 for ibuprofen. Naproxen transport was most efficient at neutral pH and was significantly enhanced upon cell treatment with TNF-α. In humans, systemically administered naproxen attained steady-state levels of 61.9 μg/mL in blood and 9.4 μg/g in healthy gingival connective tissue, while ibuprofen attained levels of 2.3 μg/mL and 1.5 μg/g, respectively. Thus, gingival fibroblasts possess transporters for NSAIDs that are up-regulated by an inflammatory mediator, but there is no evidence that they contribute to elevated NSAID levels in healthy gingiva.

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Effects of esculentoside A on turnour necrosis factor production by mice peritoneal macrophages

Mediators of Inflammation ◽

10.1155/s0962935192000565 ◽

1992 ◽

Vol 1 (6) ◽

pp. 375-377 ◽

Cited By ~ 5

Author(s):

Fang Jun ◽

Zheng Qin Yue ◽

Wang Hong Bin ◽

Ju Dian Wen ◽

Yi Yang Hua

Keyword(s):

Tumour Necrosis Factor ◽

Tumour Necrosis ◽

Inflammatory Mediator ◽

Platelet Activating Factor ◽

Peritoneal Macrophages ◽

Dependent Manner ◽

Anti Inflammatory ◽

Dose Dependent ◽

Necrosis Factor ◽

Inflammatory Effect

Esculentoside A (EsA) is a saponin isolated from the roots of Phytolacca esculenta. Previous experiments showed that it had strong anti-inflammatory effects. Tumour necrosis factor (TNF) is an important inflammatory mediator. In order to study the mechanism of the anti-inflammatory effect of EsA, it was determined whether TNF production from macrophages was altered by EsA under lipopolysaccharide (LPS) stimulated conditions. EsA was found to decrease both extracellular and cell associated TNF production in a dose dependent manner at concentrations higher than 1 μmol/l EsA. Previous studies have showed that EsA reduced the releasing of platelet activating factor (PAF) from rat macrophages. The reducing effects of EsA on the release of TNF and PAF may explain its anti-inflammatory effect.

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Anti-phosphorylcholine IgM, an Anti-inflammatory Mediator, Predicts Peripheral Vein Graft Failure: A Prospective Observational Study

European Journal of Vascular and Endovascular Surgery ◽

10.1016/j.ejvs.2018.09.010 ◽

2019 ◽

Vol 57 (2) ◽

pp. 259-266 ◽

Cited By ~ 1

Author(s):

Michael Sobel ◽

Mayumi Yagi ◽

Katherine Moreno ◽

Ted R. Kohler ◽

Gale L. Tang ◽

...

Keyword(s):

Observational Study ◽

Vein Graft ◽

Graft Failure ◽

Prospective Observational Study ◽

Inflammatory Mediator ◽

Peripheral Vein ◽

Anti Inflammatory ◽

Vein Graft Failure

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The Role of IL-37 and IL-38 in Obstetrics Abnormalities

Frontiers in Medicine ◽

10.3389/fmed.2021.737084 ◽

2021 ◽

Vol 8 ◽

Author(s):

Mei Wang

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Inflammatory Mediator ◽

Inflammatory Responses ◽

Medical Practitioners ◽

Minimal Difference ◽

Anti Inflammatory ◽

Umbilical Cords

There are two fairly common complications during pregnancy, i.e., gestational diabetes mellitus (GDM) and pre-eclampsia, which are independent, but are also closely linked in prevalence in pregnant women, with potential serious adverse consequences. IL-37 and IL-38, which belong to the IL-1 superfamily, participate in anti-inflammatory responses. Dysregulation of IL-37 and IL-38 has been observed in many auto-immune diseases. IL-37 is substantially reduced in the umbilical cords and placentas of GDM subjects, but IL-37 is significantly induced in the placentas of pre-eclampsia patients, suggesting there are differential regulatory roles of IL-37 in obstetrics, despite IL-37 being an anti-inflammatory mediator. Furthermore, IL-38 is substantially increased in the umbilical cords and placentas of GDM subjects, but minimal difference is observed in the placentas from pre-eclampsia patients. These data imply that IL-38 is also regulated independently within the diseased placentas. This review provides some insight for both basic scientists and medical practitioners to manage these patients effectively.

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