Effect of seed treatment duration on growth and colonization of Vicia faba by endophytic Beauveria bassiana and Metarhizium brunneum

2016 ◽  
Vol 103 ◽  
pp. 187-195 ◽  
Author(s):  
Lara R. Jaber ◽  
Juerg Enkerli
Keyword(s):  
Vicia Faba ◽  
Beauveria Bassiana ◽  
Treatment Duration ◽  
Seed Treatment ◽  
Metarhizium Brunneum

scholarly journals The Effect Of Seed Treatment Duration With Entomopathogenic Fungi Beauveria Bassiana On Seed Germination And Seedling Growth Of Chili

2020 ◽  
Vol 3 (1) ◽  
pp. 25-29
Author(s):  
Trizelia Trizelia
Keyword(s):  
Seed Germination ◽  
Beauveria Bassiana ◽  
Seedling Growth ◽  
Entomopathogenic Fungi ◽  
Treatment Duration ◽  
Seed Treatment ◽  
Conidial Suspension ◽  
Growth Promoters ◽  
Vigor Index ◽  
Germination And Growth

Beauveria bassiana (Balsamo) Vuill. is an entomopathogenic fungi can live  endophytic on different kinds  of plants. B. bassiana also play a role as growth promoters that  improves seed germination and growth of plant.  The effect of increased duration of seed treatment with Beauveria bassiana on seed germination and seedling parameters in chili were assessed in laboratory and field conditions. Chili seeds were soaked in the conidial suspension of B. bassiana in a concentration of 1 x 108 conidial / ml or in sterile distilled water for 3, 6, 9, and 12 h. Seed germination percentages and the vigor index were significantly affected by the application of B. bassiana. Overall, seed germination, the vigor index and plant growth of chili were significantly enhanced as seed treatment duration increased.  B. bassiana can also accelerate seed germination of chili and increase of seedling growth. Soaking the seeds with a B. bassiana suspension for 9 hours is the best time to increase of seed germination and seedling growth of chili

The entomopathogenic fungi Metarhizium brunneum and Beauveria bassiana promote systemic immunity and confer resistance to a broad range of pests and pathogens in tomato

2021 ◽  
Author(s):  
Rupali Gupta ◽  
Ravindran Keppanan ◽  
Meirav Leibman-Markus ◽  
Dalia Rav David ◽  
Yigal Elad ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Plant Growth ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plant Pathogens ◽  
Plant Immunity ◽  
Systemic Immunity ◽  
Metarhizium Brunneum ◽  
Promote Plant Growth ◽  
Arthropod Pests

Biocontrol agents can control pathogens by re-enforcing systemic plant resistance through systemic acquired resistance (SAR) or induced systemic resistance (ISR). Trichoderma spp. can activate the plant immune system through ISR, priming molecular mechanisms of defense against pathogens. Entomopathogenic fungi (EPF) can infect a wide range of arthropod pests, and play an important role in reducing pests' population. Here, we investigated the mechanisms by which EPF control plant diseases. We tested two well studied EPF, Metarhizium brunneum isolate Mb7 and Beauveria bassiana as the commercial product Velifer, for their ability to induce systemic immunity and disease resistance against several fungal and bacterial phytopathogens, and their ability to promote plant growth. We compared the activity of these EPF to an established biocontrol agent, T. harzianum T39, a known inducer of systemic plant immunity and broad disease resistance. The three fungal agents were effective against several fungal and bacterial plant pathogens and arthropod pests. Our results indicate that EPF induce systemic plant immunity and disease resistance by activating the plant host defense machinery, as evidenced by increases in reactive oxygen species (ROS) production and defense gene expression, and that EPF promote plant growth. EPF should be considered as control means for Tuta absoluta. We demonstrate that, with some exceptions, biocontrol in tomato can be equally potent by the tested EPF and T. harzianum T39, against both insect pests and plant pathogens. Taken together, our findings suggest that EPF may find use in broad-spectrum pest and disease management and as plant growth promoting agents.

scholarly journals Preventative Approach to Microbial Control of Capnodis tenebrionis by Soil Application of Metarhizium brunneum and Beauveria bassiana

Insects ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 319
Author(s):  
Dana Ment ◽  
Hysen Kokiçi ◽  
Enrico de Lillo
Keyword(s):  
Beauveria Bassiana ◽  
Microbial Control ◽  
Soil Surface ◽  
Field Application ◽  
Post Inoculation ◽  
Metarhizium Brunneum ◽  
Root Localization ◽  
Neonate Larvae ◽  
Preventative Approach ◽  
Colonization Rates

Management of the Mediterranean flat-headed root-borer, Capnodis tenebrionis, is critical due to the larvae’s root localization. Neonate larvae can be exposed to natural enemies before penetrating the roots. Application of Metarhizium brunneum strain Mb7 and Beauveria bassiana strain GHA formulations on rice granules was investigated for their efficacy against C. tenebrionis larvae. Mb7 application, evaluated on apricot twigs, significantly and dose-dependently reduced colonization rates of neonates, with highest mortality at 108 conidia/g soil. Neonate susceptibility to Mb7 and GHA was evaluated on potted rootstocks (GF677 almond × peach, 2729 plum) planted in entomopathogenic fungi (EPF)-premixed soil (1.3–1.6 × 105 conidia/cm3 soil) or in EPF-free soil surface-treated with 5 g Mb7 fungal granules (1.25 × 109 conidia). Larval colonization rates were reduced 7.4-fold in 2729 by both fungi; only Mb7 completely prevented colonization of GF677 by larvae. Larvae inside plant galleries exhibited mycosis with EPF-treated soils and both fungi proliferated on larval frass. Mb7 conidia germinated in the rhizosphere of GF677, and conidia of both fungi remained viable throughout the trial. Galleria baiting technique was used on EPF-treated soil to evaluate EPF infectivity over time; Mb7 and GHA persisted 180 and 90 days post inoculation, respectively. The formulation (fungus-covered rice grains), delivery method (mixing with soil) and persistence (3–6 months) of Mb7 and GHA are feasible for potential field application to control C. tenebrionis.

scholarly journals Effect of endophytic isolates of Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metchnikoff) Sorokin on Plutella xylostella (L.) (Lepidoptera: Plutellidae) in cabbage

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Poornesha Bathina ◽  
Ramanujam Bonam
Keyword(s):  
Beauveria Bassiana ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Seed Treatment ◽  
Foliar Application ◽  
Diamondback Moth ◽  
Main Body ◽  
Inoculation Methods ◽  
Application Method ◽  
Application Methods

Abstract Background The diamondback moth (Plutella xylostella L.) (Lepidoptera: Plutellidae) is one of the major pests in cabbage which causes severe loss to the cruciferous crops. Entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae have been established as endophytes in cabbage plants by seed treatment/root inoculation/foliar application methods in glasshouse conditions. Main body A glasshouse experiment was conducted to study the effect of endophytic isolates of B. bassiana (NBAIR Bb-5a and NBAIR Bb-45) and M. anisopliae (NBAIR Ma-4 and NBAIR Ma-35) on P. xylostella in cabbage using detached leaf bioassay method. The isolates were applied through seed treatment/root inoculation/foliar application at the concentration of 1 × 108 spores/ml and evaluated at 15, 30, 45, and 60 days after treatment (DAT) in 2017 and 2018. These isolates were colonized in cabbage leaf tissues from 15 to 60 DAT. All 4 isolates showed different mortality percentages during 15–30 DAT, and no mortality was observed after 45 and 60 DAT in different inoculation methods during both years. Among the application methods tested, foliar application method gave the highest mortality of 70–80% at 15 DAT and 12–58% at 30 DAT mortality of 2nd instar larvae of P. xylostella in cabbage. Among the isolates tested, NBAIR Ma-35 showed the highest mortality (35–79%) in all the 3 inoculation methods tested. Conclusion Endophytic isolates of B. bassiana and M. anisopliae suppressed the population of P. xylostella on cabbage leaves in three inoculation methods tested. Among all the methods tested, foliar application method showed highest mortality. These promising isolates have to be further tested under field conditions for management of P. xylostella in cabbage.

scholarly journals Effect of Beauveria bassiana-Seed Treatment on Zea mays L. Response against Spodoptera frugiperda

2021 ◽  
Vol 11 (7) ◽  
pp. 2887
Author(s):  
Laiju Kuzhuppillymyal-Prabhakarankutty ◽  
Fernando H. Ferrara-Rivero ◽  
Patricia Tamez-Guerra ◽  
Ricardo Gomez-Flores ◽  
María Cristina Rodríguez-Padilla ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Spodoptera Frugiperda ◽  
Field Trial ◽  
Seed Treatment ◽  
Insect Pest ◽  
Field Trials ◽  
Negative Control ◽  
Economic Losses ◽  
Native Strain ◽  
Symbiotic Associations

Spodoptera frugiperda is a widely distributed insect pest that causes major economic losses in various crops, particularly maize. On the other hand, Beauveria bassiana is an entomopathogenic fungus that establishes symbiotic associations with many plants and contributes to tolerance against biotic and abiotic stresses. In the present work, in laboratory experiments, the effects of the B. bassiana strain GHA, in addition to a native strain (PTG4), delivered via seed treatment in maize seedlings, were evaluated on S. frugiperda growth, development, and mortality. We inoculated maize seeds with 1 × 106B. bassiana blastospores; then these seeds were germinated and grown to seedlings under growth chamber conditions. Third-instar S. frugiperda larvae were allowed to feed on B. bassiana-treated and -untreated (negative control) seedlings until reaching the sixth instar and transferred to an artificial diet until reaching adult stage. Results showed that larvae feeding on B. bassiana strain PTG4-treated plants prolonged their larval stage. Furthermore, feeding on plants treated with B. bassiana strains yielded fewer S. frugiperda male moths compared with feeding with the untreated control plants. Under field conditions, 1 × 106 (first trial) and 1 × 108 (second trial) of B. bassiana (GHA strain) blastospores were used for corn seed inoculation. In the first field trial, there were a higher number of larvae in the negative control plants compared to those in the plants treated with B. bassiana. No larvae were found in negative control and B. bassiana-treated plants in the second field trial. In conclusion, seed treatment with B. bassiana in maize reduced S. frugiperda infestation of maize plants in field trials. S. frugiperda development was also affected in laboratory trials.

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