Design and development of a method for the reduction of infectious pathogen load and inactivation of white blood cells in whole blood products

Biologicals ◽  
2010 ◽  
Vol 38 (1) ◽  
pp. 20-30 ◽  
Author(s):  
Raymond P. Goodrich ◽  
Suzann Doane ◽  
Heather L. Reddy
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
Blood Products ◽  
Design And Development ◽  
Pathogen Load ◽  
Infectious Pathogen

Inactivation of Human White Blood Cells in Red Blood Cell Products Using the MIRASOL® System for Whole Blood.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 2897-2897
Author(s):  
Loren D. Fast ◽  
Susanne Marschner ◽  
Gilbert DiLeone ◽  
Suzann Doane ◽  
Christy Fitzpatrick ◽  
...  
Keyword(s):  
Immune Responses ◽  
T Cell Activation ◽  
Whole Blood ◽  
Blood Cells ◽  
Cell Activation ◽  
Mononuclear Cells ◽  
Protein Quality ◽  
Human Peripheral Blood ◽  
White Blood Cells ◽  
Blood Products

Abstract Transfusion of blood products containing white blood cells (WBC) can result in the induction of immune responses that can negatively impact the recipient. An approach that would mitigate these consequences would be beneficial. Previous studies had shown that exposure of platelet concentrates to light in the presence of riboflavin was able to inhibit immune responses mediated by WBC. To make this protocol more widely applicable the effect of treating whole blood units with riboflavin and varying amounts of light was tested. Human peripheral blood mononuclear cells were purified by Ficoll-Hypaque discontinuous centrifugation from aliquots of nonleukoreduced whole blood units that were untreated or exposed to Mirasol treatment using varying light dosages. Viability and phenotype of treated cells was unchanged compared to untreated controls. The results showed that exposure of whole blood units to 33J/mL red blood cells (RBC) UV light in the presence of riboflavin completely inhibited proliferation of WBC in response to polyclonal stimulators such as phytohemagglutinin, and anti-CD3/CD28 or to allogeneic stimulator cells in a mixed lymphocyte culture (MLC). Additional assays showed that treated WBC were unable to induce proliferation of normal responder cells in an MLC. Treated cells did not produce inflammatory or TH1/TH2 cytokines when stimulated with lipopolysaccharide for 24 hours or anti-CD3/CD28 for 72 hours. In addition, treatment was found to inhibit T cell activation as evidenced by the lack of CD69 expression in treated compared to untreated control cells when incubated with phorbol 12-myristate 13-acetate. These treatment conditions did not induce crossmatch incompatibility. Methemoglobin levels and hemolysis in RBC units stayed below 1% during storage for 42 days in AS-3. Platelet and plasma units separated from whole blood after treatment showed acceptable cell and protein quality over 5 days in storage or as fresh frozen plasma, respectively. In summary, Mirasol treatment was able to functionally inactivate WBC in whole blood products without adversely affecting the quality of the RBC, platelets and plasma. This technique offers potential means to achieve inactivation of WBC in whole blood units that can subsequently be separated into RBC, platelet and plasma components.

EFFECTS OF BLOOD CELLS ON PLATELET AGGREGATION BY IMPEDANCE METHOD

1987 ◽  
Author(s):  
L Mannucci ◽  
R Redaelli ◽  
E Tremoll
Keyword(s):  
Platelet Aggregation ◽  
Whole Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
Normal Subjects ◽  
Impedance Method ◽  
Induced Aggregation ◽  
Vitro Data ◽  
In Vitro Data

To evaluate the effects of blood cells on the response of platelets to aggregating agents using whole blood impedance aggregometer, studies were carried out on whole blood (WB) of normal subjects and of patients with: polycythemia vera (PV), iatrogenic anemia (IA), primary thrombocytosis (PT), idiopathic thrombotic purpura (ITP), myeloid chronic leukemia (MCL), iatrogenic leukopenia (IL). The in vitro effects of red blood cells (RBC) and of white blood cells (WBC) on platelet rich plasma (PRP) aggregation were also evaluated. WB, PRP, WBC and RBC were prepared by conventional methods. Aggregation was performed using the impedance aggregometer (mod. 540, Chrono Log Corp). In normal subjects the concentration of collagen giving 50 % aggregation (AC50 ) found in PRP did not differ from that of WB, indicating that hematocrit values within the normal range did not appreciably affect platelet aggregation. The results obtained in WB of patients are summarized in the table: In vitro data showed that aggregation in prp in wb of normal subjects was related to the number of platelets present in the sample. RBC added to PRP significant reduced aggregation only when the RBC number was greater than 4.101 cells. No effect of WBC on collagen induced aggregation of PRP was observed, whereas significant inhibition was detected after ADP. It is concluded that the aggregation evaluated in WB with impedance method is dependent on the platelet number. Also, in vitro data and studies in WB of patients indicate that aggregation is significantly affected by the presence of cells other than platelets only in conditions of changes of the ratio between platelets and leukocytes and/or red cells.

Direct freezing of whole blood enables analysis of leucocyte markers by flow cytometry: a proof-of-concept study

2021 ◽  
Author(s):  
Pénélope Bourgoin ◽  
Inès Ait Belkacem ◽  
Isabelle Arnoux ◽  
Pierre-Emmanuel Morange ◽  
Fabrice Malergue
Keyword(s):  
Flow Cytometry ◽  
Whole Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
Proof Of Concept ◽  
Fresh Blood ◽  
Blood Samples ◽  
Step Flow ◽  
Freezing Method ◽  
One Step

Aim: A new one-step flow cytometry procedure has been recently demonstrated for identifying subjects with infections, but only for fresh whole blood samples. The goal of this study was to assess its applicability on frozen samples, by proposing a new method to perform the sample freezing directly and easily. Methods: Fresh blood was tested, then frozen either directly or with dimethylsulfoxide and serum. Common markers of white blood cells as well as infection-related biomarkers were tested. Results: All percentages of leucocyte subsets and levels of infection-related biomarkers were significantly correlated between frozen and fresh samples. Conclusion: The direct freezing method enables an accurate assessment of common cellular sub-populations and of levels of important infectious biomarkers via flow cytometry.

scholarly journals Analysis of the dynamics of Staphylococcus aureus binding to white blood cells using whole blood assay and geno-to-pheno mapping

2020 ◽  
Vol 310 (3) ◽  
pp. 151411
Author(s):  
Daria Gaidar ◽  
Alice Jonas ◽  
Ruslan Akulenko ◽  
Ulla Ruffing ◽  
Mathias Herrmann ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Whole Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
Whole Blood Assay ◽  
Blood Assay

Smoothed Particle Hydrodynamics Simulations of Whole Blood in Three-Dimensional Shear Flow

2020 ◽  
Vol 17 (10) ◽  
pp. 2050009
Author(s):  
Sisi Tan ◽  
Mingze Xu
Keyword(s):  
Shear Flow ◽  
Smoothed Particle Hydrodynamics ◽  
Whole Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
Three Dimensional ◽  
Immersed Boundary ◽  
Particle Hydrodynamics ◽  
Sph Model ◽  
Smoothed Particle

Numerical modeling of whole blood still faces great challenges although significant progress has been achieved in recent decades, because of the large differences of physical and geometric properties among blood components, including red blood cells (RBCs), platelets (PLTs) and white blood cells (WBCs). In this work, we develop a three-dimensional (3D) smoothed particle hydrodynamics (SPH) model to study the whole blood in shear flow. The immersed boundary method (IBM) is used to deal with the interaction between the fluid and cells, which provides a possibility to model the RBCs, PLTs and WBCs simultaneously. The deformation of a small capsule, comparable to a PLT in size, is first examined to show the feasibility of SPH model for the PLTs’ behaviors. The motion of a single RBC in shear flow is then studied, and three typical modes, tank-treading, swinging and tumbling motions, are reproduced, which further confirm the reliability of the SPH model. After that, a simulation of the whole blood in shear flow is carried out, in which the margination trend is observed for both PLTs and WBC. This shows the capability of SPH model with IBM for the simulation of whole blood.

EFFECTS OF PLATELETS AND WHITE BLOOD CELLS AND ANTIPLATELET AGENT C7E3 (ReoproTM) ON A NEW TEST OF PAF PROCOAGULANT ACTIVITY OF WHOLE BLOOD.

1997 ◽  
Vol 86 (3) ◽  
pp. 205-219 ◽  
Author(s):  
George J Despotis ◽  
Stavroula Ikonomalou ◽  
Vladimir Levine ◽  
Diane Joiner-Maier ◽  
Samuel A Santoro ◽  
...  
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
Antiplatelet Agent ◽  
Procoagulant Activity

Preliminary results of the study on some hematological and biochemical values of Takhi

2014 ◽  
Vol 11 (2) ◽  
pp. 3-6
Author(s):  
B Bayar-Enkh ◽  
S Ganbat ◽  
P Enkhtuya
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Total Protein ◽  
Blood Cells ◽  
White Blood Cells ◽  
Total Proteins ◽  
Preliminary Results ◽  
Natural Park ◽  
Calcium And Phosphorus ◽  
Hematological Values

Some hematological values, blood total protein, protein fractions, and minerals such as calcium and phosphorus of takhi, which are now being reintroduced in Khustain natural park, were measured. There were 8.3 million red blood cells and 8000 white blood cells in 1 mm3 whole blood of takhi reintroduced in Khustain natural park, and hemoglobin was 179.6±3.2 g/l. As well, total proteins, calcium and phosphorus of takhi were 67± 0.6 g/l, 2.1±0.31 mmol/l, and 1.1±0.2 mmol/l respectively and they were similar to those in Mongolian horses. It has been necessary to investigate further adaptability of takhi in asscoation with both internal and external environments of its body.DOI: http://dx.doi.org/10.5564/mjas.v11i2.206 Mongolian Journal of Agricultural Sciences Vol.11(2) 2013 pp.3-6

Enumeration of residual white blood cells in leukoreduced blood products: Comparing flow cytometry with a portable microscopic cell counter

2016 ◽  
Vol 54 (2) ◽  
pp. 266-270 ◽  
Author(s):  
Silvia Castegnaro ◽  
Patrizia Dragone ◽  
Katia Chieregato ◽  
Alberta Alghisi ◽  
Francesco Rodeghiero ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Blood Cells ◽  
White Blood Cells ◽  
Blood Products ◽  
Cell Counter
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