Influence of anchoring ligands and particle size on the colloidal stability and in vivo biodistribution of polyethylene glycol-coated gold nanoparticles in tumor-xenografted mice

AbstractA significant area of research is biomedical applications of nanoparticles which involves efforts to control the physicochemical properties through simple and scalable processes. Gold nanoparticles have received considerable attention due to their unique properties that they exhibit based on their morphology. Gold nanospheres (AuNSs) and nanorods (AuNRs) were prepared with a seed-mediated method followed of polyethylene glycol (PEG)-coating. The seeds were prepared with 0.1 M cetyltrimethyl-ammonium bromide (CTAB), 0.005 M chloroauric acid (HAuCl4), and 0.01 M sodium borohydride (NaBH4) solution. Gold nanoparticles with spherical morphology was achieved by growth by aggregation at room temperature, while to achieve the rod morphology 0.1 M silver nitrate (AgNO3) and 0.1 M ascorbic acid solution were added. The gold nanoparticles obtained by the seed-mediated synthesis have spherical or rod shapes, depending on the experimental conditions, and a uniform particle size. Surface functionalization was developed using polyethylene glycol. Morphology, and size distribution of AuNPs were evaluated by Field Emission Scanning Electron Microscopy. The average size of AuNSs, and AuNRs was 7.85nm and 7.96 x 31.47nm respectively. Fourier transform infrared spectrometry was performed to corroborate the presence of PEG in the AuNPs surface. Additionally, suspensions of AuNSs and AuNRs were evaluated by UV-Vis spectroscopy. Gold nanoparticles were stored for several days at room temperature and it was observed that the colloidal stability increased once gold nanoparticles were coated with PEG due to the shield formed in the surface of the NPs and the increase in size which were 9.65±1.90 nm of diameter for AuNSs and for AuNRs were 29.03±5.88 and 8.39±1.02 nm for length and transverse axis, respectively.

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Ultrasmall Gold Nanoparticles Behavior in Vivo Modulated by Surface Polyethylene Glycol (PEG) Grafting

Bioconjugate Chemistry ◽

10.1021/acs.bioconjchem.6b00488 ◽

2016 ◽

Vol 28 (1) ◽

pp. 239-243 ◽

Cited By ~ 14

Author(s):

Shuaidong Huo ◽

Shizhu Chen ◽

Ningqiang Gong ◽

Juan Liu ◽

Xianlei Li ◽

...

Keyword(s):

Gold Nanoparticles ◽

Polyethylene Glycol

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Development of Bio-Functionalized, Raman Responsive, and Potentially Excretable Gold Nanoclusters

Nanomaterials ◽

10.3390/nano11092181 ◽

2021 ◽

Vol 11 (9) ◽

pp. 2181

Author(s):

Ryan D. Mellor ◽

Andreas G. Schätzlein ◽

Ijeoma F. Uchegbu

Keyword(s):

Particle Size ◽

Gold Nanoparticles ◽

Ethylene Glycol ◽

Gold Nanoclusters ◽

Physiological Conditions ◽

Non Invasive ◽

In Vivo Detection ◽

Raman Response ◽

Octadecyl Amine

Gold nanoparticles (AuNPs) are used experimentally for non-invasive in vivo Raman monitoring because they show a strong absorbance in the phototherapeutic window (650–850 nm), a feature that is accompanied by a particle size in excess of 100 nm. However, these AuNPs cannot be used clinically because they are likely to persist in mammalian systems and resist excretion. In this work, clustered ultrasmall (sub-5 nm) AuNP constructs for in vivo Raman diagnostic monitoring, which are also suitable for mammalian excretion, were synthesized and characterized. Sub-5 nm octadecyl amine (ODA)-coated AuNPs were clustered using a labile dithiol linker: ethylene glycol bis-mercaptoacetate (EGBMA). Upon clustering via a controlled reaction and finally coating with a polymeric amphiphile, a strong absorbance in the phototherapeutic window was demonstrated, thus showing the potential suitability of the construct for non-invasive in vivo detection and monitoring. The clusters, when labelled with a biphenyl-4-thiol (BPT) Raman tag, were shown to elicit a specific Raman response in plasma and to disaggregate back to sub-5 nm particles under physiological conditions (37 °C, 0.8 mM glutathione, pH 7.4). These data demonstrate the potential of these new AuNP clusters (Raman NanoTheranostics—RaNT) for in vivo applications while being in the excretable size window.

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SYNTHESIS AND STABILITY OF RESVERATROL-CONJUGATED GOLD NANOPARTICLES MODIFIED WITH POLYETHYLENE GLYCOL

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2020.v12s1.ff050 ◽

2020 ◽

pp. 230-236

Author(s):

RADITYA ISWANDANA ◽

RICHA NURSELVIANA ◽

SUTRIYO SUTRIYO

Keyword(s):

Particle Size ◽

Gold Nanoparticles ◽

Polyethylene Glycol ◽

Zeta Potential ◽

High Performance ◽

Entrapment Efficiency ◽

Test Results ◽

Novel Method ◽

The Stability ◽

Phosphate Buffered Saline

Objective: Gold nanoparticles (AuNPs) are highly useful for drug delivery, but their application is limited by their stability as they readily aggregate.This issue can be prevented by adding a stabilizing agent such as resveratrol (RSV), which is a polyphenol derived from plants, that is used to preventcancer. Therefore, we propose a novel method to prepare stable RSV-conjugated nanoparticles modified with polyethylene glycol (RSV-AuNP-PEG).Methods: In the first step, the Turkevich method was used to synthesize the AuNPs. Then, PEG was added as stabilizer agent and conjugated with RSV.The synthesized conjugates were characterized using ultraviolet-visible spectrophotometry, Fourier transform infrared spectroscopy, particle sizeanalysis, and high-performance liquid chromatography.Results: The obtained RSV-AuNP-PEG had a particle size of 83.93 nm with a polydispersity index (PDI) of 0.562 and formed a translucent purple-redfluid in solution. The zeta potential was −22.9 mV, and the highest entrapment efficiency was 75.86±0.66%. For comparison, the RSV-AuNP solutionwas purple and turbid, the particle size was 51.97 nm with a PDI of 0.694, and the zeta potential was −24.6 mV. The stability test results showed thatthe storage stability of RSV-AuNP-PEG was better than that of AuNP-RSV. Further, the RSV-AuNP-PEG was shown to be most stable in 2% bovine serumalbumin (BSA) while the AuNP-RSV was most stable in 2% BSA in phosphate-buffered saline pH 7.4.Conclusion: These results show that modification of RSV-conjugated AuNPs with PEG effectively prevents their aggregation in storage, but only incertain mediums.

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Optical Nonlinear Properties of Gold Nanoparticles Synthesized by Laser Ablation in Polymer Solution

Journal of Nanomaterials ◽

10.1155/2017/4803843 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 7

Author(s):

M. Tajdidzadeh ◽

A. B. Zakaria ◽

Z. Abidin Talib ◽

A. S. Gene ◽

S. Shirzadi

Keyword(s):

Particle Size ◽

Gold Nanoparticles ◽

Polyethylene Glycol ◽

Laser Ablation ◽

Volume Fraction ◽

Chitosan Solution ◽

The Other ◽

Distilled Water ◽

Nonlinear Properties ◽

Glycol Solution

In the present study, gold nanoparticles were synthesized in various polymer solutions by means of employing laser ablation technique at the same ablation time. Specifically, gold nanoparticles were synthesized in polyethylene glycol and chitosan solutions, in order to compare the effects of the liquid media which served as stabilizers for particle size and volume fraction of nanoparticles. In addition, this experiment was repeated in distilled water for reference purposes. As the findings indicated, the particle size which was obtained in polyethylene glycol was about 7.49 nm, that is, smaller than those of chitosan solution and distilled water, respectively. In contrast, it was observed that the volume fraction of gold nanoparticles increased in polyethylene glycol in comparison with the other media which indicated an effect on the formation of NPs. On the other hand,Z-scan technique was employed to measure the nonlinear refractive index and nonlinear absorption coefficient of nanofluids containing gold nanoparticles. Consequently, the nonlinear properties of nanofluids pointed to a significant contribution with the number of nanoparticles observed in fluids and both optical nonlinear parameters were observed to increase by means of a prior increase in the volume fraction of Au-NPs in polyethylene glycol solution.

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Synthesis of Monodispersed Gold Nanoparticles with Exceptional Colloidal Stability with Grafted Polyethylene Glycol-g-polyvinyl Alcohol

Journal of Nanomaterials ◽

10.1155/2015/712359 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 14

Author(s):

Alaaldin M. Alkilany ◽

Alaa I. Bani Yaseen ◽

Mohammed H. Kailani

Keyword(s):

Gold Nanoparticles ◽

Polyethylene Glycol ◽

Polyvinyl Alcohol ◽

Colloidal Stability ◽

Kinetic Mechanism ◽

Grafted Copolymer ◽

Synthesis Parameters ◽

Salt Addition ◽

One Step ◽

Spherical Gold Nanoparticles

Herein, we report the synthesis of spherical gold nanoparticles with tunable core size (23–79 nm) in the presence of polyethylene glycol-g-polyvinyl alcohol (PEG-g-PVA) grafted copolymer as a reducing, capping, and stabilizing agent in a one-step protocol. The resulted PEG-g-PVA-capped gold nanoparticles are monodispersed with an exceptional colloidal stability against salt addition, repeated centrifugation, and extensive dialysis. The effect of various synthesis parameters and the kinetic/mechanism of the nanoparticle formation are discussed.

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Photochemical transformations of thiolated polyethylene glycol coatings on gold nanoparticles

Environmental Science Nano ◽

10.1039/c6en00141f ◽

2016 ◽

Vol 3 (5) ◽

pp. 1090-1102 ◽

Cited By ~ 4

Author(s):

Stacey M. Louie ◽

Justin M. Gorham ◽

Eric A. McGivney ◽

Jingyu Liu ◽

Kelvin B. Gregory ◽

...

Keyword(s):

Gold Nanoparticles ◽

Polyethylene Glycol ◽

Gold Nanoparticle ◽

Uv Irradiation ◽

Colloidal Stability ◽

Chain Scission ◽

Photochemical Reactions ◽

Surface Coatings ◽

Photochemical Transformations ◽

Thiolated Polyethylene Glycol

Photochemical reactions can cause significant transformations of manufactured nanomaterials and their surface coatings in sunlit environments. In this study, loss of thiolated polyethylene glycol from gold nanoparticle surfaces by chain scission was observed under UV irradiation and resulted in diminished colloidal stability.

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Gold Nanoparticles Endowed with Low-Temperature Colloidal Stability by Cyclic Polyethylene Glycol in Ethanol

Soft Matter ◽

10.1039/d1sm00720c ◽

2021 ◽

Author(s):

M. Ali Aboudzadeh ◽

Joscha Kruse ◽

Maria Sanromán-Iglesias ◽

Daniele Cangialosi ◽

Ángel Alegría ◽

...

Keyword(s):

Gold Nanoparticles ◽

Polyethylene Glycol ◽

Low Temperature ◽

Thermal Behavior ◽

Metal Nanoparticles ◽

Polymer Brushes ◽

Colloidal Stability ◽

Solution Temperature ◽

Critical Solution Temperature ◽

Upper Critical Solution Temperature

The colloidal stability of metal nanoparticles is tremendously dependent on the thermal behavior of polymer brushes. Neat polyethylene glycol (PEG) presents an unconventional upper critical solution temperature in ethanol, where...

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Preparation of pegylated nano-liposomal formulation containing SN-38: In vitro characterization and in vivo biodistribution in mice

Acta Pharmaceutica ◽

10.2478/v10007-009-0020-0 ◽

2009 ◽

Vol 59 (2) ◽

pp. 133-144 ◽

Cited By ~ 47

Author(s):

Fatemeh Atyabi ◽

Anahita Farkhondehfai ◽

Farnaz Esmaeili ◽

Rassoul Dinarvand

Keyword(s):

Particle Size ◽

Encapsulation Efficiency ◽

Liposomal Formulation ◽

Pegylated Liposomes ◽

In Vivo Biodistribution ◽

In Vitro Characterization ◽

Poorly Soluble ◽

Low Toxicity

Preparation of pegylated nano-liposomal formulation containing SN-38:In vitrocharacterization andin vivobiodistribution in mice7-Ethyl-10-hydroxy-camptothecin (SN-38), a metabolite of irinotecan x HCl, is poorly soluble in aqueous solutions and practically insoluble in most physiologically compatible and pharmaceutically acceptable solvents. Formulation of SN-38 in concentrated pharmaceutical delivery systems for parenteral administration is thus very difficult. Due to their biocompatibility and low toxicity, liposomes were considered for the delivery of SN-38. In this study, pegylated liposomes with distearoylphosphatidylcholine, distearoylphosphatidylethanolamine containing SN-38 were prepared and their characteristics, such as particle size, encapsulation efficiency,in vitrodrug release and biodistribution, were investigated. The particle size of liposomes was in the range of 150--200 nm. The encapsulation efficiency andin vitrorelease rate of pegylated liposomes was higher than those of non-pegylated liposomes. As expected, the distribution of pegylated liposomes in body organs such as liver, kidney, spleen and lung was considerably lower than that of non-pegylated liposomes. Also, their blood concentration was at least 50 % higher than that of non-pegylated liposomes.

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Engineering of glycyrrhizin capped gold nanoparticles for liver targeting: in vitro evaluation and in vivo biodistribution study

RSC Advances ◽

10.1039/c6ra05202a ◽

2016 ◽

Vol 6 (51) ◽

pp. 44944-44954 ◽

Cited By ~ 5

Author(s):

Shaivee Borker ◽

Milind Patole ◽

Alpana Moghe ◽

Varsha Pokharkar

Keyword(s):

Gold Nanoparticles ◽

Antiviral Drug ◽

Biodistribution Study ◽

Liver Targeting ◽

In Vitro Evaluation ◽

In Vivo Biodistribution

Glycyrrhizin reduced and stabilized gold nanoparticles as carriers of antiviral drug lamivudine. The presence of glycyrrhizin enhanced uptake and localization of drug loaded gold nanoparticles in hepatocytes.

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