scholarly journals Multivariate analysis as a tool for selecting the vine pruning pretreatment towards the highest enzymatic hydrolysis yield

2020 ◽  
Vol 140 ◽  
pp. 105653
Author(s):  
Elenilson G. Alves Filho ◽  
Tatiane C. Maciel ◽  
Eduardo Gudiña ◽  
Emilio C. Miguel ◽  
Ligia R. Rodrigues ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Enzymatic Hydrolysis ◽  
Hydrolysis Yield

Optimization of olive pomace enzymatic hydrolysis for fermentable sugar production

2016 ◽  
Vol 46 (6) ◽  
pp. 778-790 ◽  
Author(s):  
Ghassan Abo Chameh ◽  
Fadi Kheder ◽  
Francois Karabet
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ph Value ◽  
Maximum Yield ◽  
Olive Pomace ◽  
Mixing Rate ◽  
Content Type ◽  
Hydrolysis Yield ◽  
Pretreatment Time ◽  
Hydrolysis Conditions ◽  
Hydrolysis Of

Purpose The purpose of this paper was to find out the appropriate enzymatic hydrolysis conditions of alkali pretreated olive pomace (OP) which enable maximum yield of reducing sugar. Design/methodology/approach The commercial enzymatic preparation (Viscozyme® L) was used for the hydrolysis of OP. The effects of pretreatment, time, temperature, pH, enzyme quantity and substrate loading on the hydrolysis yield were investigated. Findings This study showed that enzymatic hydrolysis of OP using Viscozyme® L can be successfully performed at 50°C. Alkaline pretreatment step of OP prior the enzymatic hydrolysis was indispensable. The hydrolysis yield of alkaline pretreated OP was 2.6 times higher than the hydrolysis yield of untreated OP. Highest hydrolysis yield (33.5 ± 1.5 per cent) was achieved after 24 h using 1 per cent (w/v) OP load in the presence of 100 μl Viscozyme® L at 50°C and pH 5.5 with mixing rate of 100 rpm (p = 0.05). Originality/value Reaction time, temperature, pH value and enzyme quantity were found to have a significant effect on enzymatic hydrolysis yield of alkali pretreated of OP. Although high-solid loadings of OP lowered the hydrolysis yield, it produced higher concentration of reducing sugars, which may render the OP conversion process more economically feasible.

scholarly journals Enzymatic sugar production from elephant grass and reed straw through pretreatments and hydrolysis with addition of thioredoxin-His-S

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Xianqin Lu ◽  
Can Li ◽  
Shengkui Zhang ◽  
Xiaohan Wang ◽  
Wenqing Zhang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Pennisetum Purpureum ◽  
Commercial Application ◽  
Hydrolysis Lignin ◽  
Cellulose Content ◽  
Sugar Production ◽  
Elephant Grass ◽  
Reed Straw ◽  
Hydrolysis Yield ◽  
Hydrolysis Of

Abstract Background The bioconversion of lignocellulose to fermentable C5/C6-saccharides is composed of pretreatment and enzymatic hydrolysis. Lignin, as one of the main components, resists lignocellulose to be bio-digested. Alkali and organosolv treatments were reported to be able to delignify feedstocks and loose lignocellulose structure. In addition, the use of additives was an alternative way to block lignin and reduce the binding of cellulases to lignin during hydrolysis. However, the relatively high cost of these additives limits their commercial application. Results This study explored the feasibility of using elephant grass (Pennisetum purpureum) and reed straw (Phragmites australis), both of which are important fibrous plants with high biomass, no-occupation of cultivated land, and soil phytoremediation, as feedstocks for bio-saccharification. Compared with typical agricultural residues, elephant grass and reed straw contained high contents of cellulose and hemicellulose. However, lignin droplets on the surface of elephant grass and the high lignin content in reed straw limited their hydrolysis performances. High hydrolysis yield was obtained for reed straw after organosolv and alkali pretreatments via increasing cellulose content and removing lignin. However, the hydrolysis of elephant grass was only enhanced by organosolv pretreatment. Further study showed that the addition of bovine serum albumin (BSA) or thioredoxin with His- and S-Tags (Trx-His-S) improved the hydrolysis of alkali-pretreated elephant grass. In particular, Trx-His-S was first used as an additive in lignocellulose saccharification. Its structural and catalytic properties were supposed to be beneficial for enzymatic hydrolysis. Conclusions Elephant grass and reed straw could be used as feedstocks for bioconversion. Organosolv and alkali pretreatments improved their enzymatic sugar production; however, the increase in hydrolysis yield of pretreated elephant grass was not as effective as that of reed straw. During the hydrolysis of alkali-pretreated elephant grass, Trx-His-S performed well as additive, and its structural and catalytic capability was beneficial for enzymatic hydrolysis.

scholarly journals Enzymatic Hydrolysis of Water Extractable Polysaccharides from Leaves of Plantago major L.

Folia Medica ◽  
2017 ◽  
Vol 59 (2) ◽  
pp. 210-216 ◽  
Author(s):  
Paolina K. Lukova ◽  
Diana P. Karcheva-Bahchevanska ◽  
Veselin P. Bivolarski ◽  
Rumen D. Mladenov ◽  
Ilia N. Iliev ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Degree Of Polymerization ◽  
Hot Water ◽  
Cell Mediated Immunity ◽  
Plantago Major ◽  
Beneficial Effects ◽  
Hydrolysis Yield ◽  
Antiviral Activities ◽  
Hydrolysis Of ◽  
Water Extractable

AbstractBackground:Plantago majorL. leaves have been used for centuries by the traditional medicine in the treatment of infectious disorders of the respiratory, urinary and digestive tracts. Researchers have reported that hot water extracts ofPlantago majorpossess a broad-spectrum of anticancer, antioxidant and antiviral activities, as well as activities which modulate cell-mediated immunity. Their beneficial properties may be due to the significant content of polysaccharides. The polysaccharides that have been isolated from the leaves ofPlantago majorL. have different structures – pectic substances, galactans, arabinogalactans, glucomannans.Aim:The aim of this paper was to study the correlation between the structure of the water extractable polysaccharides isolated fromPlantago majorL. leaves and their enzymatic hydrolysis with different carbohydrate hydrolases.Materials and methods:The hydrolysis reactions were performed with the enzymes hemicellulase and mannanase. Spectrophotometric total reducing sugars assay was used to examine the hydrolysis yield. The monosaccharide and oligosaccharide compositions were determined using HPLC analysis.Results:The highest hydrolysis yield of the water extractable polysaccharides fromPlantago majorleaves was obtained by treatment with hemicellulase. The hydrolysis yield increased with the augmentation of the ratio of enzyme to polysaccharide. Galactose was the prevalent monosaccharide identified in the composition of the isolated polysaccharides. Oligosaccharides with different degree of polymerization were also detected.Conclusion:The enzymatic hydrolysis of water extractable polysaccharides fromPlantago majorleaves allows us to obtain different types of oligosaccharides with beneficial effects on both human health and industry.

Research on Pretreatment Process and Product Detection in Enzymatic Hydrolysis of Lignocellulosic Biomass

2012 ◽  
Vol 503-504 ◽  
pp. 190-193
Author(s):  
Jing Jing Zhao ◽  
Hai Yan Yang ◽  
Bo Li ◽  
Fu Ming Chen
Keyword(s):  
Weight Loss ◽  
Enzymatic Hydrolysis ◽  
Lignocellulosic Biomass ◽  
Dilute Acid Pretreatment ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Positive Correlation ◽  
Hydrolysis Yield ◽  
First Time ◽  
Hydrolysis Of

Sulfite pretreatment was explored in enzymatic hydrolysis of bagasse for the first time, and was compared with dilute acid pretreatment. The results showed that enzymatic hydrolysis yield of bagasse after sulfite treatment was lower than that of the bagasse pretreated with dilute acid. Meanwhile, complexity of sulfite pretreatment and its high cost made it infeasible for industrialized production. Results also showed positive correlation of bagasse pretreatment weight loss to enzymatic hydrolysis yield in dilute acid pretreatment processes, which made substrate weight loss a plausible parameter in pretreatment evaluation.

Enhancing enzymatic hydrolysis yield of sweet sorghum straw polysaccharides by heavy ion beams irradiation pretreatment

2019 ◽  
Vol 222 ◽  
pp. 114976 ◽  
Author(s):  
Fuqiang Xu ◽  
Junkai Wang ◽  
Miaoyin Dong ◽  
Shuyang Wang ◽  
Guoqing Xiao ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sweet Sorghum ◽  
Heavy Ion ◽  
Ion Beams ◽  
Hydrolysis Yield ◽  
Sorghum Straw

scholarly journals Co-production of Xylooligosaccharides and Xylose From Poplar Sawdust by Recombinant Endo-1,4-β-Xylanase and β-Xylosidase Mixture Hydrolysis

2021 ◽  
Vol 8 ◽  
Author(s):  
Qi Li ◽  
Yunpeng Jiang ◽  
Xinyi Tong ◽  
Linguo Zhao ◽  
Jianjun Pei
Keyword(s):  
Enzymatic Hydrolysis ◽  
Deep Understanding ◽  
Important Application ◽  
Raw Material ◽  
Hydrolysis Yield ◽  
Wood Polysaccharides ◽  
Optimized Conditions ◽  
Rate Limiting ◽  
Hydrolysis Of ◽  
Poplar Sawdust

As is well-known, endo-1,4-β-xylanase and β-xylosidase are the rate-limiting enzymes in the degradation of xylan (the major hemicellulosic component), main functions of which are cleavaging xylan to release xylooligosaccharides (XOS) and xylose that these two compounds have important application value in fuel, food, and other industries. This study focuses on enzymatic hydrolysis of poplar sawdust xylan for production of XOS and xylose by a GH11 endo-1,4-β-xylanase MxynB-8 and a GH39 β-xylosidase Xln-DT. MxynB-8 showed excellent ability to hydrolyze hemicellulose of broadleaf plants, such as poplar. Under optimized conditions (50°C, pH 6.0, dosage of 500 U/g, substrate concentration of 2 mg/mL), the final XOS yield was 85.5%, and the content of XOS2−3 reached 93.9% after 18 h. The enzymatic efficiency by MxynB-8 based on the poplar sawdust xylan in the raw material was 30.5%. Xln-DT showed excellent xylose/glucose/arabinose tolerance, which is applied as a candidate to apply in degradation of hemicellulose. In addition, the process and enzymatic mode of poplar sawdust xylan with MxynB-8 and Xln-DT were investigated. The results showed that the enzymatic hydrolysis yield of poplar sawdust xylan was improved by adding Xln-DT, and a xylose-rich hydrolysate could be obtained at high purity, with the xylose yield of 89.9%. The enzymatic hydrolysis yield was higher (32.2%) by using MxynB-8 and Xln-DT together. This study provides a deep understanding of double-enzyme synergetic enzymolysis of wood polysaccharides to valuable products.

scholarly journals Effect of Intermediate Washing on Ozonolysis Delignification and Enzymatic Hydrolysis of Wheat Straw

2021 ◽  
Author(s):  
Ali Kamel H. Al Jibouri
Keyword(s):  
Enzymatic Hydrolysis ◽  
Water Content ◽  
Wheat Straw ◽  
Initial Water Content ◽  
Optimal Parameters ◽  
Initial Water ◽  
Hydrolysis Yield ◽  
Fiber Size ◽  
Two Stages ◽  
Hydrolysis Of

Wheat straw was pretreated with ozone to increase the enzymatic hydrolysis yield. Ozonolysis pretreatment was performed in two stages with an intermediate washing step with water in between. Part of the delignification products (lignin fragments) were removed by a washing step so ozone was used to oxidize more lignin rather than oxidizing lignin fragments. Three parameters, i.e. Initial Water Content (IWC) in wheat straw, Washing Starting Time (WST), and Washing Contact Time (WCT), were optimized to minimize Acid Insoluble Lignin (AIL) content of ozonated wheat straw. Performing an experiment using optimal parameters' values, i.e. IWC equal to 45 wt. %, WST equal to 20 minutes and WCT equal to 80 seconds, showed a drop in AIL content to 9.35 % with an increase in enzymatic hydrolysis yield to 80 % of the theoretical. Readjusting water content of wheat straw to 45 % before performing the 2nd ozonolysis stage further reduced AIL content to 7.36 % and increased the hydrolysis yield to 90 %. Increasing wheat straw fiber size from < 2 mm to < 6 mm decreased the hydrolysis yield to 72 %. All results were considered significant improvements when compared to untreated wheat straw with AIL content of 20.5 % and hydrolysis yield of 23 %.

scholarly journals Effect of Intermediate Washing on Ozonolysis Delignification and Enzymatic Hydrolysis of Wheat Straw

2021 ◽  
Author(s):  
Ali Kamel H. Al Jibouri
Keyword(s):  
Enzymatic Hydrolysis ◽  
Water Content ◽  
Wheat Straw ◽  
Initial Water Content ◽  
Optimal Parameters ◽  
Initial Water ◽  
Hydrolysis Yield ◽  
Fiber Size ◽  
Two Stages ◽  
Hydrolysis Of

Wheat straw was pretreated with ozone to increase the enzymatic hydrolysis yield. Ozonolysis pretreatment was performed in two stages with an intermediate washing step with water in between. Part of the delignification products (lignin fragments) were removed by a washing step so ozone was used to oxidize more lignin rather than oxidizing lignin fragments. Three parameters, i.e. Initial Water Content (IWC) in wheat straw, Washing Starting Time (WST), and Washing Contact Time (WCT), were optimized to minimize Acid Insoluble Lignin (AIL) content of ozonated wheat straw. Performing an experiment using optimal parameters' values, i.e. IWC equal to 45 wt. %, WST equal to 20 minutes and WCT equal to 80 seconds, showed a drop in AIL content to 9.35 % with an increase in enzymatic hydrolysis yield to 80 % of the theoretical. Readjusting water content of wheat straw to 45 % before performing the 2nd ozonolysis stage further reduced AIL content to 7.36 % and increased the hydrolysis yield to 90 %. Increasing wheat straw fiber size from < 2 mm to < 6 mm decreased the hydrolysis yield to 72 %. All results were considered significant improvements when compared to untreated wheat straw with AIL content of 20.5 % and hydrolysis yield of 23 %.

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