Sonapatha (Oroxylum Indicum) Mediates Cytotoxicity In Cultured Hela Cells by Inducing Apoptosis and Suppressing NF-ΚB, COX-2, RASSF7 and NRF2

2021 ◽  
pp. 105126
Author(s):  
Khawlhring Lalrinzuali ◽  
Mathipi Vabeiryureilai ◽  
Ganesh Chandra Jagetia
Keyword(s):  
Hela Cells ◽  
Oroxylum Indicum ◽  
Cox 2

scholarly journals Seminal Plasma Promotes the Expression of Tumorigenic and Angiogenic Genes in Cervical Adenocarcinoma Cells via the E-Series Prostanoid 4 Receptor

Endocrinology ◽  
2006 ◽  
Vol 147 (7) ◽  
pp. 3356-3365 ◽  
Author(s):  
Melissa Muller ◽  
Kurt J. Sales ◽  
Arieh A. Katz ◽  
Henry N. Jabbour
Keyword(s):  
Growth Factor ◽  
Seminal Plasma ◽  
Hela Cells ◽  
Cervical Adenocarcinoma ◽  
Dominant Negative Mutant ◽  
Mapk Kinase ◽  
Ep4 Receptor ◽  
Adenocarcinoma Cells ◽  
Cox 2 ◽  
Angiogenic Genes

E-series prostanoid (EP)4 receptor is up-regulated in numerous cancers, including cervical carcinomas, and has been implicated in mediating the effects of prostaglandin (PG)E2 in tumorigenesis. In addition to regulation by endogenously biosynthesized PGE2, neoplastic cervical epithelial cells in sexually active women may also be regulated by PGs present in seminal plasma. In this study, we investigated the signal transduction pathways mediating the role of seminal plasma and PGE2 in the regulation of tumorigenic and angiogenic genes via the EP4 receptor in cervical adenocarcinoma (HeLa) cells. HeLa cells were stably transfected with EP4 receptor in the sense orientation. Seminal plasma and PGE2 signaling via the EP4 receptor induced the activation of cyclooxygenase (COX)-2 and vascular endothelial growth factor (VEGF) promoters, expression of COX-2 and VEGF mRNA and protein, and secretion of VEGF protein into the culture medium. Treatment of HeLa cells with seminal plasma or PGE2 also rapidly induced the phosphorylation of ERK1/2 via the EP4 receptor. Preincubation of cells with a specific EP4 receptor antagonist (ONO-AE2-227) or chemical inhibitors of epidermal growth factor receptor (EGFR) tyrosine kinase or MAPK kinase or cotransfection of cells with dominant-negative mutant cDNA targeted against the EGFR, serine/threonine kinase Raf, or MAPK kinase abolished the EP4-induced activation of COX-2, VEGF, and ERK1/2. Therefore, we have demonstrated that seminal plasma and PGE2 can promote the expression of tumorigenic and angiogenic factors, in cervical adenocarcinoma cells via the EP4 receptor, EGFR, and ERK1/2 signaling pathways.

Activated PI3K/Akt/COX-2 Pathway Induces Resistance to Radiation in Human Cervical Cancer HeLa Cells

2010 ◽  
Vol 25 (3) ◽  
pp. 317-323 ◽  
Author(s):  
Shu Xia ◽  
Yin Zhao ◽  
Shiying Yu ◽  
Mengxian Zhang
Keyword(s):  
Cervical Cancer ◽  
Hela Cells ◽  
Cox 2 ◽  
Human Cervical Cancer

scholarly journals Proapoptotic activities of Oroxylum indicum leave extract in HeLa cells

2019 ◽  
Vol 9 (8) ◽  
pp. 339
Author(s):  
NorFazila Che Mat ◽  
NurulHidayah Wahab ◽  
NurAfina Mohd Din ◽  
YeeYing Lim ◽  
NoorIzani Noor Jamil
Keyword(s):  
Hela Cells ◽  
Oroxylum Indicum

scholarly journals Dexamethasone Causes Sustained Expression of Mitogen-Activated Protein Kinase (MAPK) Phosphatase 1 and Phosphatase-Mediated Inhibition of MAPK p38

2002 ◽  
Vol 22 (22) ◽  
pp. 7802-7811 ◽  
Author(s):  
Marina Lasa ◽  
Sonya M. Abraham ◽  
Christine Boucheron ◽  
Jeremy Saklatvala ◽  
Andrew R. Clark
Keyword(s):  
Protein Kinase ◽  
Hela Cells ◽  
Interleukin 1 ◽  
Cell Types ◽  
Dose Dependence ◽  
Mitogen Activated Protein Kinase ◽  
Dependent Manner ◽  
Similar Dose ◽  
Mitogen Activated Protein ◽  
Cox 2

ABSTRACT The stress-activated protein kinase p38 stabilizes a number of mRNAs encoding inflammatory mediators, such as cyclooxygenase 2 (Cox-2). In HeLa cells the anti-inflammatory glucocorticoid dexamethasone destabilizes Cox-2 mRNA by inhibiting p38 function. Here we demonstrate that this effect is phosphatase dependent. Furthermore, in HeLa cells dexamethasone induced the sustained expression of mitogen-activated protein kinase phosphatase 1 (MKP-1), a potent inhibitor of p38 function. The inhibition of p38 and the induction of MKP-1 by dexamethasone occurred with similar dose dependence and kinetics. No other known p38 phosphatases were induced by dexamethasone, and other cell types which failed to express MKP-1 also failed to inhibit p38 in response to dexamethasone. The proinflammatory cytokine interleukin 1 (IL-1) induced MKP-1 expression in a p38-dependent manner and acted synergistically with dexamethasone to induce MKP-1 expression. In HeLa cells treated with IL-1 or IL-1 and dexamethasone, the dynamics of p38 activation mirrored the expression of MKP-1. These observations suggest that MKP-1 participates in a negative-feedback loop which regulates p38 function and that dexamethasone may inhibit proinflammatory gene expression in part by inducing MKP-1 expression.

The COX-2 inhibitor NS398 selectively sensitizes hypoxic HeLa cells to ionising radiation by mechanisms both dependent and independent of COX-2

2020 ◽  
Vol 148 ◽  
pp. 106422 ◽  
Author(s):  
Shailendra Anoopkumar-Dukie ◽  
Tom Conere ◽  
Aileen Houston ◽  
Liam King ◽  
David Christie ◽  
...  
Keyword(s):  
Hela Cells ◽  
Ionising Radiation ◽  
Cox 2

scholarly journals The Antitumor Effects of Triterpenoid Saponins from theAnemone flaccidaand the Underlying Mechanism

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Lin-Tao Han ◽  
Ying Fang ◽  
Ming-Ming Li ◽  
Hong-Bing Yang ◽  
Fang Huang
Keyword(s):  
Hela Cells ◽  
Hepatoma Cell ◽  
Underlying Mechanism ◽  
Antitumor Activities ◽  
Antitumor Effects ◽  
Triterpenoid Saponins ◽  
Hepatoma Cell Lines ◽  
Cox 2 ◽  
Preventive Role

Anemone flaccidaFr. Schmidt, a family of ancient hopanoids, have been used as traditional Asian herbs for the treatments of inflammation and convulsant diseases. Previous study on HeLa cells suggested that triterpenoid saponins fromAnemone flaccidaFr. Schmidt may have potential antitumor effect due to their apoptotic activities. Here, we confirmed the apoptotic activities of the following five triterpenoid saponins: glycoside St-I4a (1), glycoside St-J (2), anhuienoside E (3), hedera saponin B (4), and flaccidoside II (5) on human BEL-7402 and HepG2 hepatoma cell lines, as well as the model of HeLa cells treated with lipopolysaccharide (LPS). We found that COX-2/PGE2 signaling pathway, which plays key roles in the development of cancer, is involved in the antitumor activities of these saponins. These data provide the evidence that triterpenoid saponins can induce apoptosis via COX-2/PGE2 pathway, implying a preventive role of saponins fromAnemone flaccidain tumor.

Inhibition of Ovalitenin A on Proliferation of HeLa Cells via Apoptosis, G2/M Cell Cycle Arrest, and Down-regulation of COX-2

2016 ◽  
Vol 8 (3) ◽  
pp. 259-266
Author(s):  
Dong-yu Liu ◽  
Yan Guo ◽  
Jian-yong Si ◽  
Gui-bo Sun ◽  
Bing Zhang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Hela Cells ◽  
M Cell ◽  
Down Regulation ◽  
Cycle Arrest ◽  
Cox 2

Platinum Compounds as Stains for Electron Microscopy

1974 ◽  
Vol 32 ◽  
pp. 230-231
Author(s):  
S. K. Aggarwal ◽  
P. McAllister ◽  
R. W. Wagner ◽  
B. Rosenberg
Keyword(s):  
Electron Microscopy ◽  
Hela Cells ◽  
Uranyl Acetate ◽  
Sarcoma 180 ◽  
Platinum Compounds ◽  
Kb Cells ◽  
En Bloc ◽  
Human Lymphoma ◽  
Ultrathin Sections ◽  
Blue Coloration

Uranyl acetate has been used as an electron stain for en bloc staining as well as for staining ultrathin sections in conjunction with various lead stains (Fig. 1). Present studies reveal that various platinum compounds also show promise as electron stains. Certain platinum compounds have been shown to be effective anti-tumor agents. Of particular interest are the compounds with either uracil or thymine as one of the ligands (cis-Pt(II)-uracil; cis-Pt(II)-thymine). These compounds are amorphous, highly soluble in water and often exhibit an intense blue coloration. These compounds show enough electron density to be used as stains for electron microscopy. Most of the studies are based on various cell lines (human AV, cells, human lymphoma cells, KB cells, Sarcoma-180 ascites cells, chick fibroblasts and HeLa cells) while studies on tissue blocks are in progress.

Electron Microscopy of Mycoplasma Pneumoniae

1971 ◽  
Vol 29 ◽  
pp. 258-259 ◽  
Author(s):  
E. S. Boatman ◽  
G. E. Kenny
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Growth Phase ◽  
Hela Cells ◽  
Sulfonic Acid ◽  
Gamma Globulin ◽  
Horse Serum ◽  
Morphological Aspects ◽  
The Family

Information concerning the morphology and replication of organism of the family Mycoplasmataceae remains, despite over 70 years of study, highly controversial. Due to their small size observations by light microscopy have not been rewarding. Furthermore, not only are these organisms extremely pleomorphic but their morphology also changes according to growth phase. This study deals with the morphological aspects of M. pneumoniae strain 3546 in relation to growth, interaction with HeLa cells and possible mechanisms of replication.The organisms were grown aerobically at 37°C in a soy peptone yeast dialysate medium supplemented with 12% gamma-globulin free horse serum. The medium was buffered at pH 7.3 with TES [N-tris (hyroxymethyl) methyl-2-aminoethane sulfonic acid] at 10mM concentration. The inoculum, an actively growing culture, was filtered through a 0.5 μm polycarbonate “nuclepore” filter to prevent transfer of all but the smallest aggregates. Growth was assessed at specific periods by colony counts and 800 ml samples of organisms were fixed in situ with 2.5% glutaraldehyde for 3 hrs. at 4°C. Washed cells for sectioning were post-fixed in 0.8% OSO4 in veronal-acetate buffer pH 6.1 for 1 hr. at 21°C. HeLa cells were infected with a filtered inoculum of M. pneumoniae and incubated for 9 days in Leighton tubes with coverslips. The cells were then removed and processed for electron microscopy.

Use of Uranium-Labeled Antibodies for Electron Microscopic Localization of Various Antigens in Mammalian Cells

1967 ◽  
Vol 25 ◽  
pp. 136-137
Author(s):  
J. P. Petrali ◽  
E. J. Donati ◽  
L. A. Sternberger
Keyword(s):  
Endoplasmic Reticulum ◽  
Hela Cells ◽  
Mammalian Cells ◽  
Specific Antiserum ◽  
Electron Microscopic ◽  
E Coli ◽  
Cytoplasmic Membranes ◽  
Viral Progeny ◽  
Ultrathin Sections ◽  
Electron Microscopic Localization

Specific contrast is conferred to subcellular antigen by applying purified antibodies, exhaustively labeled with uranium under immunospecific protection, to ultrathin sections. Use of Seligman’s principle of bridging osmium to metal via thiocarbohydrazide (TCH) intensifies specific contrast. Ultrathin sections of osmium-fixed materials were stained on the grid by application of 1) thiosemicarbazide (TSC), 2) unlabeled specific antiserum, 3) uranium-labeled anti-antibody and 4) TCH followed by reosmication. Antigens to be localized consisted of vaccinia antigen in infected HeLa cells, lysozyme in monocytes of patients with monocytic or monomyelocytic leukemia, and fibrinogen in the platelets of these leukemic patients. Control sections were stained with non-specific antiserum (E. coli).In the vaccinia-HeLa system, antigen was localized from 1 to 3 hours following infection, and was confined to degrading virus, the inner walls of numerous organelles, and other structures in cytoplasmic foci. Surrounding architecture and cellular mitochondria were unstained. 8 to 14 hours after infection, antigen was localized on the outer walls of the viral progeny, on cytoplasmic membranes, and free in the cytoplasm. Staining of endoplasmic reticulum was intense and focal early, and weak and diffuse late in infection.

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