Synergistic antidiabetic activity of Taraxacum officinale (L.) Weber ex F.H.Wigg and Momordica charantia L. polyherbal combination

The aim of this study was to evaluate fresh dandelion leaves on turkey performance. The study used a complete randomized design of 4 treatments and 6 replications. A total number of 24 turkeys (Meleagris gallapavo)-12 week-old were used in this 5 weeks of feed trial. The experimental animals were fed with the diet containing 37% of commercial broiler starter diet, 40% rice bran and forage (water hyacinth and fresh dandelion leaves). The treatments with fresh dandelion leaves addition were P0: 0%, P1: 5%, P2: 10%, and P3: 15%. The variables observed were bio active compound in dandelion leaves, daily behavior and turkey performance. Data were analyzed by Analysis of Variance (ANOVA) and the significant differences between treatments were analyzed by Duncan's Multiple Range Test. The results showed that dandelion leaves contain flavonoids, tannins, saponins and steroids, vitamin D were 17.37 ppm and antioxidants IC50 have an antioxidant activity with a value of 102.31. Observation of daily behavior showed that turkeys behavior was dominated by eating and resting activities, where the highest frequency of resting activities was 0.43 per second (P0). Turkey performance were not significantly different on feed consumption, carcass weight, carcass percentage for all treatments but significantly different (p<0.05) on average daily gain (AVG) and feed conversion ratio. Therefore it can be concluded that fresh dendelion leaves are able to used up to 15% in turkey diet. Key words: antioxidant, dandelion, turkey performance, vitamin D DAFTAR PUSTAKA Azhari NT & Apliriana E. 2016. Peranan jombang (Taraxacum officinale) sebagai hepatoprotektor. Jurnal Majority. 5(5): 32-36. Astuti S. 2008. Isoflavon kedelai dan potensinya sebagai penangkap radikal bebas. Jurnal Teknologi Industri dan Hasil Pertanian. 13(2): 126-136. Adawiah, Sukandar D & Muawanah A. 2015. Aktivitas antioksidan dan kandungan komponen bioaktif sari buah namnam. Jurnal Penelitian dan Pengembangan Ilmu Kimia. 1(2): 130-136. Amin M, Sawhney SS & Jassal MMS. 2013. Qualitative and quantitative analysis of phytochemicals of Taraxacum officinale. Wudpecker Journal of Pharmacy and Pharmocology. 2(1):1-5. Badarinath A, Rao K, Chetty CS, Ramkanth S, Rajan T & Gnanaprakash K. 2010. A review on in vitro antioxidant methods: comparisons, correlations and considerations. International Journal of Pharmatical Technology Research. 2(2): 1276-1285. Cook R, Xin H & Nettleton D. 2005. Effects of cage stocking density of feeding behaviours of groups housed laying hens. Journal Animals Agriculture Biology. 49(1): 187-192. Dheer R & Bhatnager P. 2010. A study of the antidiabetic activity of Barleria prionitis linn. Indian Journal of Phamacology. 42(2): 1-5. Gibril S, Samsel S RM, Yassin OE, Hassan AA & Atta M. 2013. Body gain and carcas charateristics of turkeys (Meleagris gallopavo) under extensive system of management in the student. International Journal of Applied Poultry Research. 2(2): 23-26. Mishra A, Kaone P, Schouten W, Sprujit B & Metz J. 2005. Tempral and sequential structure of behaviour and facility usage of laying hens in an encrihed environment. Poultry Science. 84:979-991. Rizky A, Haryono D & Kasymir. 2016. Analisis usaha dan strategi pengembangan ternak kalkun mitra alam kabupaten pringsewu provinsi Lampung. Jurnal Ilmu Ilmu Agribisnis. 4(3): 235-242. Ramian F & Indrianti MA. 2018. Analisa potensi eceng gondok (Eichornia crassipes) Danau Limboto sebagai pakan ternak. Prosiding Seminar Nasional Integrated Farming System. Gorontalo (ID): Universitas Muhammadiyah Gorontalo. Setiawan B. 2017. Kandungan protein kasar dan serat kasar dedak padi yang difermentasi dengan mikroorganisme lokal [skripsi]. Makassar (ID): Universitas Hasanuddin. Steel RGD & Torrie JH. 1993. Prinsip dan Prosedur Statistika Suatu Pendekatan Biometrik. Sumatri B, penerjemah. Terjemah dari: Principles and Procedures of Statistics. Jakarta (ID): Gramedia Pustaka Utama, Suprijatna E, Sunarti D, Mahfudz L D, Ardiningsasi S M, Inayah A & Purnomo A H S. 2010. Performans produksi dan efisiensi penggunaan protein ransum pada kalkun lokal yang dipelihara secara intensif diberi ransum mengandung daun kobis (Brassica oleracea var capitata) afkir. Seminar Nasional Unggas Lokal. Semarang (ID): Fakultas Peternakan, Universitas Diponegoro. Wulan, Yudistira A, & Rotinsulu H. 2019. Uji aktivitas antioksidan dari ekstrak etanol daun Mimosa pudica Linn. menggunakan metode DPPH. Jurnal Ilmiah Farmasi. 8(1): 106-113.

Download Full-text

In vivo and in vitro antidiabetic potential of Taraxacum officinale root extracts

Current Issues in Pharmacy and Medical Sciences ◽

10.2478/cipms-2020-0030 ◽

2020 ◽

Vol 33 (3) ◽

pp. 168-175

Author(s):

Lana YM. Juee ◽

Alaadin M. Naqishbandi

Keyword(s):

Glucose Tolerance ◽

Glucose Uptake ◽

Aqueous Extract ◽

Taraxacum Officinale ◽

Antidiabetic Activity ◽

Total Phenolic ◽

Diabetic Mice ◽

Phytochemical Study

AbstractTaraxacum officinale F.H. Wigg (Asteraceae) root is traditionally used to treat diabetes, dyspepsia, heartburn, anorexia and hepatitis. In this work, petroleum ether, chloroform, methanol and aqueous extracts of T. officinale root were evaluated for their antidiabetic activity in normoglycemic and alloxan-induced diabetic mice at two concentrations (200 and 400 mg/kg) using antidiabetic and subcutaneous glucose tolerance tests. Herein, in vitro glucose uptake assay was performed using HepG2 and 2-NDBG, while LC-MS/MS was employed for the phytochemical study of the main active constituents in the active extract. In the experiments, T. officinale root aqueous extract (400 mg/kg) showed a significant decrement in blood glucose level (62.33%, p ≤0.05), while other extracts (p >0.05) showed insignificant activity – in alloxan-induced diabetic mice with no apparent effect on the normoglycemic model. The extracts also showed an insignificant reduction in glucose levels (p >0.05) in the subcutaneous glucose tolerance test. However, a significant glucose uptake enhancement (149.6724%, p ≤0.05) was exhibited by the aqueous extract. Phytochemical study of the aqueous extract showed higher total phenolic than total flavonoid content, in which chlorogenic acid, protocatechuic acid, and luteolin-7-glucoside were identified.

Download Full-text

Antioxidant and Antidiabetic Activity of Aqueous Ethanolic Extract of Momordica charantia L. (Cucurbitaceaevar Guti) Fruit in Streptozotocin (STZ) Induced Diabetes Rats

Asian Journal of Biochemistry ◽

10.3923/ajb.2017.59.70 ◽

2017 ◽

Vol 12 (2) ◽

pp. 59-70

Author(s):

Samir Abd-elmonem Ah Ismail ◽

Rehab Farouk Mohamme Ali ◽

Mohamed Diaa el-Dein El-Maksoud ◽

Amgad Kamal Hasan ◽

Ayman Mohammed El-Anany

Keyword(s):

Ethanolic Extract ◽

Momordica Charantia ◽

Antidiabetic Activity ◽

Aqueous Ethanolic Extract

Download Full-text

Cucurbitane-type triterpenoids from the vines of Momordica charantia and their anti-inflammatory, cytotoxic, and antidiabetic activity

Phytochemistry ◽

10.1016/j.phytochem.2021.113026 ◽

2022 ◽

Vol 195 ◽

pp. 113026

Author(s):

Chia-Ching Liaw ◽

Hung-Tse Huang ◽

Hui-Kang Liu ◽

Yu-Chi Lin ◽

Li-Jie Zhang ◽

...

Keyword(s):

Momordica Charantia ◽

Antidiabetic Activity ◽

Anti Inflammatory

Download Full-text

Exploring Antioxidant and Enzymes (A-Amylase and B-Glucosidase) Inhibitory Activity of Morinda lucida and Momordica charantia Leaves from Benin

Foods ◽

10.3390/foods9040434 ◽

2020 ◽

Vol 9 (4) ◽

pp. 434 ◽

Cited By ~ 4

Author(s):

Michaelle Chokki ◽

Mihaela Cudălbeanu ◽

Cheikna Zongo ◽

Durand Dah-Nouvlessounon ◽

Ioana Otilia Ghinea ◽

...

Keyword(s):

Inhibitory Activity ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Momordica Charantia ◽

Antidiabetic Activity ◽

Array Detector ◽

Scavenging Activity ◽

Inhibition Assay ◽

Medicinal Uses

Background: Momordica charantia Linn. (Cucurbitaceae), the wild variety of bitter melon and Morinda lucida Benth (Rubiaceae) were commonly used as a popular folk medicine in Benin. This research focused to measure the antioxidant and enzyme inhibitory effects of M. charantia and M. lucida leaves and their antidiabetic activity. Methods: Antioxidant activities were evaluated by micro-dilution technique using DPPH free radical scavenging activity and β-carotene-linoleate bleaching assay. The α-amylase inhibition assay was carried out utilizing the 3,5-dinitrosalicylic acid procedure, while β-glucosidase inhibition assay was demonstrated using as substrate p-nitrophenyl-β-D-glucopyranoside (PNPG). HPLC-DAD analysis was realized using a high-performance liquid chromatography systems with diode-array detector, L-3000. Results: Chlorogenic acid, epicatechin, daidzein, rutin, naringin, quercetin, naringenin and genistein were identified as polyphenol compounds in the both plants extract. Dichloromethane and ethyl acetate extracts showed a good α-amylase inhibitory activity (56.46 ± 1.96% and 58.76 ± 2.74% respectively). M. lucida methanolic extract has shown IC50 of 0.51 ± 0.01 mg/mL, which is the lowest for DPPH scavenging activity. M. lucida dichloromethane extract showed the highest inhibitory capacity of β-glucosidase activity (82.11. ± 2.15%). Conclusion: These results justify some traditional medicinal uses of both plants. The purified fractions could be used in future formulations, possibly incorporated in functional foods to combat certain diseases.

Download Full-text

Structure modeling and antidiabetic activity of a seed protein of Momordica charantia in non-obese diabetic (NOD) mice

Bioinformation ◽

10.6026/97320630009766 ◽

2013 ◽

Vol 9 (15) ◽

pp. 766-770 ◽

Cited By ~ 6

Author(s):

Gagan Chhabra ◽

◽

Aparna Dixit

Keyword(s):

Seed Protein ◽

Nod Mice ◽

Momordica Charantia ◽

Antidiabetic Activity ◽

Structure Modeling

Download Full-text

Análisis del contenido fúngico de algunas especies vegetales cultivadas en Ecuador

FACSALUD-UNEMI ◽

10.29076/issn.2602-8360vol3iss4.2019pp3-12p ◽

2019 ◽

Vol 3 (4) ◽

pp. 3-12

Author(s):

Mariuxi Medina ◽

Haydelba D'Armas ◽

Carmita Jaramillo-Jaramillo ◽

Diana San Martín

Keyword(s):

Moringa Oleifera ◽

Taraxacum Officinale ◽

Momordica Charantia ◽

Coriandrum Sativum ◽

Melissa Officinalis ◽

Cymbopogon Citratus ◽

Ageratum Conyzoides ◽

Artemisia Absinthium ◽

Borago Officinalis

El uso de plantas medicinales es una de las terapias médicas más antiguas y extendidas que se remonta a los tiempos prehistóricos, y en la actualidad también las plantas son utilizadas en la preparación de fitofármacos con opciones para curar enfermedades. El ensayo de la determinación de hongos se llevó a cabo en la Planta Piloto de Farmacia (tratamiento de las hojas de las especies vegetales) y el Laboratorio de Microbiología (determinación de hongos de las especies vegetales, utilizando medio de cultivo denominado agar Sabouraud más la muestra vegetal), de la Unidad Académica de Ciencias Químicas y de la Salud, de la Universidad Técnica de Machala. Posteriormente, se realizó el conteo de colonias, tanto macroscópica, que se determina en el medio de cultivo de la siembra, como microscópica, para identificar las formas germinativas usando azul lactofenol. El procedimiento se repitió por duplicado, para obtener réplicas de los datos de los resultados. Se evaluó la determinación del contenido total de hongos de las siguientes especies vegetales: Cymbopogon citratus (hierba luisa), Melissa officinalis (toronjil), Taraxacum officinale (diente de león), Artemisia absinthium (ajenjo), Piper carpunya (guaviduca), Moringa oleífera (moringa), Coriandrum sativum (cilantro), Momordica charantia (achochilla), Borago officinalis (borraja), Aloysia citriodora (cedrón), Ambrosia artemisifolia (altamisa) y Ageratum conyzoides (mastranto). Los resultados obtenidos mostraron que todas las muestras de las doce especies vegetales estudiadas presentaron desarrollo de hongos filamentosos, con una gran variabilidad de los mismos, dentro de los límites permisibles y contemplados por el Instituto Ecuatoriano de Normalización (INEN), encontrándose aptos como materia prima para su utilización en la elaboración de nutracéuticos y productos medicinales o fitofármacos; siendo A. conyzoides (mastranto) la única especie que supera la normativa en el promedio de diluciones.

Download Full-text

Penggunaan Dandelion (Taraxacum officinale) dalam Pakan Terhadap Performa Kalkun (Meleagris gallopavo)

Jurnal Ilmu Nutrisi dan Teknologi Pakan ◽

10.29244/jintp.v18i1.30538 ◽

2020 ◽

Vol 18 (1) ◽

pp. 19-23

Author(s):

H D S Tadjudin ◽

M Shofiah Shofiah ◽

S Aini Aini ◽

Widya Hermana ◽

Arif Darmawan

Keyword(s):

Vitamin D ◽

Laying Hens ◽

Average Daily Gain ◽

Meleagris Gallopavo ◽

Taraxacum Officinale ◽

Feed Consumption ◽

Antidiabetic Activity ◽

Feed Conversion ◽

Daily Behavior

The aim of this study was to evaluate fresh dandelion leaves on turkey performance. The study used a complete randomized design of 4 treatments and 6 replications. A total number of 24 turkeys (Meleagris gallapavo)-12 week-old were used in this 5 weeks of feed trial. The experimental animals were fed with the diet containing 37% of commercial broiler starter diet, 40% rice bran and forage (water hyacinth and fresh dandelion leaves). The treatments with fresh dandelion leaves addition were P0: 0%, P1: 5%, P2: 10%, and P3: 15%. The variables observed were bio active compound in dandelion leaves, daily behavior and turkey performance. Data were analyzed by Analysis of Variance (ANOVA) and the significant differences between treatments were analyzed by Duncan's Multiple Range Test. The results showed that dandelion leaves contain flavonoids, tannins, saponins and steroids, vitamin D were 17.37 ppm and antioxidants IC50 have an antioxidant activity with a value of 102.31. Observation of daily behavior showed that turkeys behavior was dominated by eating and resting activities, where the highest frequency of resting activities was 0.43 per second (P0). Turkey performance were not significantly different on feed consumption, carcass weight, carcass percentage for all treatments but significantly different (p<0.05) on average daily gain (AVG) and feed conversion ratio. Therefore it can be concluded that fresh dendelion leaves are able to used up to 15% in turkey diet. Key words: antioxidant, dandelion, turkey performance, vitamin D DAFTAR PUSTAKA Azhari NT & Apliriana E. 2016. Peranan jombang (Taraxacum officinale) sebagai hepatoprotektor. Jurnal Majority. 5(5): 32-36. Astuti S. 2008. Isoflavon kedelai dan potensinya sebagai penangkap radikal bebas. Jurnal Teknologi Industri dan Hasil Pertanian. 13(2): 126-136. Adawiah, Sukandar D & Muawanah A. 2015. Aktivitas antioksidan dan kandungan komponen bioaktif sari buah namnam. Jurnal Penelitian dan Pengembangan Ilmu Kimia. 1(2): 130-136. Amin M, Sawhney SS & Jassal MMS. 2013. Qualitative and quantitative analysis of phytochemicals of Taraxacum officinale. Wudpecker Journal of Pharmacy and Pharmocology. 2(1):1-5. Badarinath A, Rao K, Chetty CS, Ramkanth S, Rajan T & Gnanaprakash K. 2010. A review on in vitro antioxidant methods: comparisons, correlations and considerations. International Journal of Pharmatical Technology Research. 2(2): 1276-1285. Cook R, Xin H & Nettleton D. 2005. Effects of cage stocking density of feeding behaviours of groups housed laying hens. Journal Animals Agriculture Biology. 49(1): 187-192. Dheer R & Bhatnager P. 2010. A study of the antidiabetic activity of Barleria prionitis linn. Indian Journal of Phamacology. 42(2): 1-5. Gibril S, Samsel S RM, Yassin OE, Hassan AA & Atta M. 2013. Body gain and carcas charateristics of turkeys (Meleagris gallopavo) under extensive system of management in the student. International Journal of Applied Poultry Research. 2(2): 23-26. Mishra A, Kaone P, Schouten W, Sprujit B & Metz J. 2005. Tempral and sequential structure of behaviour and facility usage of laying hens in an encrihed environment. Poultry Science. 84:979-991. Rizky A, Haryono D & Kasymir. 2016. Analisis usaha dan strategi pengembangan ternak kalkun mitra alam kabupaten pringsewu provinsi Lampung. Jurnal Ilmu Ilmu Agribisnis. 4(3): 235-242. Ramian F & Indrianti MA. 2018. Analisa potensi eceng gondok (Eichornia crassipes) Danau Limboto sebagai pakan ternak. Prosiding Seminar Nasional Integrated Farming System. Gorontalo (ID): Universitas Muhammadiyah Gorontalo. Setiawan B. 2017. Kandungan protein kasar dan serat kasar dedak padi yang difermentasi dengan mikroorganisme lokal [skripsi]. Makassar (ID): Universitas Hasanuddin. Steel RGD & Torrie JH. 1993. Prinsip dan Prosedur Statistika Suatu Pendekatan Biometrik. Sumatri B, penerjemah. Terjemah dari: Principles and Procedures of Statistics. Jakarta (ID): Gramedia Pustaka Utama, Suprijatna E, Sunarti D, Mahfudz L D, Ardiningsasi S M, Inayah A & Purnomo A H S. 2010. Performans produksi dan efisiensi penggunaan protein ransum pada kalkun lokal yang dipelihara secara intensif diberi ransum mengandung daun kobis (Brassica oleracea var capitata) afkir. Seminar Nasional Unggas Lokal. Semarang (ID): Fakultas Peternakan, Universitas Diponegoro. Wulan, Yudistira A, & Rotinsulu H. 2019. Uji aktivitas antioksidan dari ekstrak etanol daun Mimosa pudica Linn. menggunakan metode DPPH. Jurnal Ilmiah Farmasi. 8(1): 106-113.

Download Full-text