Evaluation of antidiabetic activity  of the ethanol extract of Momordica charantia L. and the identification of charantine by gas chromatography coupled with Mass spectrometry

Objective: The objective of the study was to evaluate brine shrimp lethality of Blighia sapida stem-bark extract and its fractions and identify the bioactive constituents in the ethyl acetate fraction (EAF) using gas chromatography-mass spectrometry (GC-MS) technique. Methods: The ethanol extract (EE) and its fractions were subjected to lethality assay, and GC-MS analysis of EAF was carried out. Results: The lethality test showed a concentration-dependent mortality rate in the brine shrimp nauplii for the EE and its fractions. GC-MS analysis of EAF of the extract revealed the existence of 13 peaks of the GC-MS chromatogram with only one prominent compound, n-hexadecanoic acid (peak area of 10.13%). Conclusion: The result revealed the presence of 13 bioactive components in the EAF of the extract, the majority of which have been reported for different biological activities, hence, justifies the use of the plant in the treatment and management of different diseases ethnomedicinally.

Download Full-text

Identification of Secondary Metabolites from the Ethanol extract of the leaves of Bauhinia tomentosa by GC-MS Analysis

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00482 ◽

2021 ◽

pp. 2335-2341

Author(s):

R. Balabhaskar ◽

K. Vijayalakshmi

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Secondary Metabolites ◽

Mass Spectra ◽

Ethanol Extract ◽

Alpha Tocopherol ◽

Gas Chromatography Mass Spectrometry ◽

Chemical Components ◽

Chromatography Mass Spectrometry ◽

Ms Analysis

Gas Chromatography-Mass Spectrometry (GC-MS) is normally used for direct analysis of chemical components existing in herbal medicines. The medicinal plants are having numerous bioactive components which are identified even at less than 1ng by using GC-MS or LC-MS analysis. The aim of this study is to identify the secondary metabolites present in the leaves of B. tomentosa using gas chromatography-mass spectrometry (GC-MS) analysis. In the present study the ethanol extract of the leaves of Bauhinia tomentosa has been subjected to GC-MS analysis, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis revealed the presence of 14 secondary metabolites. These compounds were identified by comparing their retention times and peak areas with those from the literature and by interpretation of the mass spectra. The major secondary metabolites were DL-.alpha.-tocopherol (14.84%), 2-[(trimethylsilyl oxy]-, methyl ester, 1-alpha,2-alpha.-epoxy-1-beta-methylcholesta-4,6-dien-3-one (12.93%), pentacosenoic acid (12.71%), phytol (10.28%), Ethyl Isoallocholate (8.197%), Spirost-8-en-11-one-3-hydroxy-,(3-beta,5 alpha,14 beta,20 beta, 22 beta,25R)-(8.162%), Urs-12-en-28-ol (6.675%), 1-Octadecyne (5.702%) and Cholest-8-en-3-beta-ol,Acetate (5.426%). The compounds having area less that 5% were considered of no significance. These findings suggest that the presence of these secondary metabolites may be the cause for the properties exhibited by Bauhinia tomentosa. Thus, presence of various bioactive compounds justifies the use of the leaf for various ailments by traditional practitioners.

Download Full-text

EKSTRAKSI DAN IDENTIFIKASI PENDAHULUAN GOLONGAN SENYAWA FENOL DARI RIMPANG LENGKUAS MERAH (Alpinia purpurata (Vieill) K. Sch)

Jurnal Sains Natural ◽

10.31938/jsn.v1i2.19 ◽

2017 ◽

Vol 1 (2) ◽

pp. 111

Author(s):

Nia Yuliani ◽

Amry Syawaalz ◽

Mawaddah Lisna

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Phenolic Compounds ◽

Skin Diseases ◽

Ethanol Extract ◽

Molecular Formula ◽

Phenol Red ◽

Food Manufacturing ◽

Red Ginger ◽

Alpinia Purpurata

Extraction and Identification of Phenol Compounds Group Introction galanga Rhizome RED (Alpinia purpurata (Vieill) K. Sch) Indonesia is a country rich in natural resources with a variety of crops are grown, one of which is a spice plant. Plant herb is a plant that is used in addition to the food manufacturing process is also used as potential drugs medicines, such that the red ginger (Alpinia purpurata (Vieill) K. Sch). Red ginger is a plant that has been known to have the potential to cure many diseases. Generally, people use the red ginger to treat diarrhea and skin diseases caused by fungi. Ginjer contains phenol red that could serve as an antibacterial. The study was conducted to extract the phenolic compounds and identify them by Gas Chromatography and Mass Spectrometry (GCMS), with the following steps: extraction of samples, testing of phenolic compounds, and identification of phenolic compounds by GCMS. According to the research and identification was carried out on the red rhizome ethanol extract, we could conclude that the phytochemical tests showed positive rhizome containing phenol red. And from the GCMS got some phenolic compounds contained in the ethanol extract of rhizome of red, one compound with a molecular weight of 164 g / mol with molecular formula C10H12O2 .Keyword : Red gingge (Alpinia purpurata (Vieill) K. Sch), compound fenol, extraction, GCMS. ABSTRAK Indonesia merupakan negara yang kaya dengan sumber daya alam dengan berbagai jenis tanaman yang tumbuh, salah satu diantaranya adalah tanaman rempah - rempah. Tanaman rempah merupakan tanaman yang dimanfaatkan selain untuk proses pembuatan makanan juga cukup potensial dimanfaatkan sebagai obat – obatan, diantaranya yaitu lengkuas merah (Alpinia purpurata (Vieill) K. Sch). Lengkuas merah merupakan tanaman yang telah diketahui berpotensi dapat mengobati berbagai macam penyakit. Umumnya masyarakat menggunakan lengkuas merah untuk mengobati diare dan penyakit kulit yang disebabkan oleh jamur. Lengkuas merah mengandung senyawa fenol yang dapat berfungsi sebagai antibakteri. Penelitian ini dilakukan untuk mengekstraksi senyawa fenol dan mengidentifikasinya dengan Gas Chromatography dan Mass Spectrometry (GCMS), dengan melalui beberapa tahapan yaitu ekstraksi sampel, uji senyawa fenolik, dan identifikasi senyawa fenolik dengan GCMS. Berdasarkan hasil penelitian dan identifikasi yang telah dilakukan pada ekstrak etanol rimpang lengkuas merah, dapat disimpulkan bahwa uji fitokimia menunjukkan rimpang lengkuas merah positif mengandung senyawa fenol. Dan dari hasil GCMS didapatkan beberapa senyawa fenol yang terkandung pada ekstrak etanol rimpang lengkuas merah, salah satunya senyawa dengan berat molekul 164 g/mol yang mempunyai rumus molekul C10H12O2.Kata kunci : Lengkuas merah (Alpinia purpurata (Vieill) K. Sch), senyawa fenol, ekstraksi, GCMS.

Download Full-text

GC–MS analysis and antibacterial activities of Moringa oleifera leaf extracts on selected clinical bacterial isolates

Bulletin of the National Research Centre ◽

10.1186/s42269-021-00640-9 ◽

2021 ◽

Vol 45 (1) ◽

Author(s):

Kingsley Erhons Enerijiofi ◽

Funmilayo Hannah Akapo ◽

Joseph Omorogiuwa Erhabor

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Pathogenic Bacteria ◽

Moringa Oleifera ◽

Ethanol Extract ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Bacterial Isolates ◽

Leaf Extracts ◽

Chromatography Mass Spectrometry

Abstract Background Man has a long history of utilizing herbal preparations to treat infections. Therefore, this study aimed to investigate the quantitative phytochemical components, gas chromatography–mass spectrometry analysis, and the antibacterial properties of the aqueous and ethanol leaf extracts of Moringa oleifera on some clinical bacterial isolates. Results Aqueous and ethanol extractions from Moringa oleifera yielded 40.75% and 62.87%, respectively. Flavonoid (20.76 mg/100 g) was the highest, while saponin (2.00 mg/100 g) was the least of all phytochemicals detected. The proximate nutrient composition revealed that carbohydrate (46.59%) had the highest, while lipid (7.37%) was the least. Eleven compounds were detected in both extracts by gas chromatography–mass spectrometry. The eleven compounds identified had higher concentrations in the ethanol extract except 2-octenoic (26.09 mg/kg) acid and 1, 2-epoxyhexadecane (8.84 mg/kg) in aqueous extract which were considerably higher than 0.62 mg/kg and < 0.01 mg/kg in ethanol extract. The minimum inhibitory concentration and minimum bactericidal concentration were 6.25 mg/ml against the test organisms for ethanol extract. Conclusion The antibacterial activity of the ethanol extract was more active against the bacterial isolates than the aqueous, which increased as the extract concentration increases. The reports revealed that Moringa oleifera is an all-important herb that can inhibit infections from the studied pathogenic bacteria isolates.

Download Full-text