Methyl jasmonate- or gibberellins A3-induced astaxanthin accumulation is associated with up-regulation of transcription of β-carotene ketolase genes (bkts) in microalga Haematococcus pluvialis

2010 ◽  
Vol 101 (16) ◽  
pp. 6468-6474 ◽  
Author(s):  
Yandu Lu ◽  
Peng Jiang ◽  
Shaofang Liu ◽  
Qinhua Gan ◽  
Hongli Cui ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Haematococcus Pluvialis ◽  
Regulation Of Transcription ◽  
Β Carotene

scholarly journals The Effect of Methyl Jasmonate Vapour on Some Characteristics of Fruit Ripening, Carotenoids and Tomatine Changes in Tomato (Lycopersicon esculentum Mill.)

2013 ◽  
Vol 48 (2) ◽  
pp. 27-35 ◽  
Author(s):  
Janusz Czapski ◽  
Marian Saniewski
Keyword(s):  
Lycopersicon Esculentum ◽  
Methyl Jasmonate ◽  
Fruit Ripening ◽  
Scoring System ◽  
Outer Part ◽  
Lycopersicon Esculentum Mill ◽  
Visual Scoring ◽  
Lycopene Content ◽  
Fruit Pericarp ◽  
Β Carotene

Tomato ripening in normal red-fruited cultivar (Fiorin) was delayed by treatment with methyl jasmonate (JA-Me) vapour. A visual scoring system for describing tomato ripening was used. Surface of fruits exposed to JA-Me vapour, increased in yellow and decreased in red as determined by HunterLab colour meter. JA-Me significantly altered the firmness of fruits after 21 days storage. Vapour of JA-Me enhanced the level of β-carotene in outer part (peel with 3 mm pericarp tissue) of fruit, while it had no effect in peeled fruit pericarp. JA-Me treatment decreased the level of lycopene in outer part and pericarp tissue, however, in outer part lycopene content decreased at a higher rate than in pericarp. Amount of tomatine in fruits treated with JA-Me had enhanced four-fold in outer part and by 62% in peeled fruit pericarp as compared with the control.

scholarly journals EFFECT OF NaCl ON HYEMATOCOCCUS PLUVIALIS PRODUCTIVITY AND CONTENT IN ITS CELLS OF PHOTOSYNTHETIC PIGMENTS, ACTIVE OXYGEN FORMS AND ASTAXANTINE

2018 ◽  
Vol 63 (3) ◽  
pp. 263-275 ◽  
Author(s):  
N. G. Averina ◽  
N. V. Kozel ◽  
R. A. Sherbakov ◽  
M. S. Radyuk ◽  
E. E. Manankina ◽  
...  
Keyword(s):  
Photosynthetic Pigments ◽  
Haematococcus Pluvialis ◽  
Total Content ◽  
Dry Weight ◽  
Control Culture ◽  
Maximum Effect ◽  
Cell Diameter ◽  
Chl A ◽  
Dry Biomass ◽  
Β Carotene

The effect of NaCl (25, 50, 100, 200 and 300 mM) on the productivity of the Haematococcus pluvialis strain IBCE H-17 on such parameters as dry biomass, content of protein, photosynthetic pigments, as well as astaxanthin and reactive oxygen species (ROS) was studied. It was found that NaCl at low and medium concentrations (25, 50 and 100 mM) in the culture medium stimulated the accumulation of dry biomass during the first 7 days of cultivation on average 1,3 times as compared to the control (Rudik’s standard medium). After 12 days of incubation, stimulation averaged 33 % using 25 and 50 mM salt. The protein content on a dry weight basis fell, averaging 70 % of the control on the 7th day of incubation with 50–300 mM salt and 55 % on the 12th day for a salt concentration of 100–300 mM. When the algae was grown for 7 days on solutions containing NaCl, the total content of photosynthetic pigments – chlorophylls (Chl) a and b as well as the carotenoids neoxanthine, violaxanthin, lutein and β-carotene decreased. Chl b was more resistant to salinity than Chl a. Of all the pigments, NaCl exerts the greatest negative effect on β-carotene. Stress conditions created by NaCl led to the generation of ROS, in particular, after 7 days of cultivation the total ROS content in the “NaCl-100” variant was 1.7 times higher than that in the control culture and 3.0 times higher than the control in the 12-day culture. A significant positive effect of salinity on the content of astaxanthin was noted. The maximum effect was observed with 100 mM NaCl. After 7 days of incubation, the content of astaxanthin exceeded the control indices by 2.8 times, and after 12 days – by 20.5 times. The number of algae cells after 7 days of incubation in the “NaCl-100” variant decreased on average by 33 %, while the cell diameter increased by 29 %. 

scholarly journals Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli

1997 ◽  
Vol 324 (2) ◽  
pp. 421-426 ◽  
Author(s):  
Susumu KAJIWARA ◽  
Paul D. FRASER ◽  
Keiji KONDO ◽  
Norihiko MISAWA
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Genomic Library ◽  
Haematococcus Pluvialis ◽  
Isoprenoid Biosynthesis ◽  
Nucleotide Sequence Analysis ◽  
Efficient Production ◽  
Isopentenyl Diphosphate ◽  
E Coli ◽  
Β Carotene

Escherichia coli expressing the Erwinia carotenoid biosynthesis genes, crtE, crtB, crtI and crtY, form yellow-coloured colonies due to the presence of β-carotene. This host was used as a visible marker for evaluating regulatory systems operating in isoprenoid biosynthesis of E. coli. cDNAs enhancing carotenoid levels were isolated from the yeast Phaffia rhodozyma and the green alga Haematococcus pluvialis. Nucleotide sequence analysis indicated that they coded for proteins similar to isopentenyl diphosphate (IPP) isomerase of the yeast Saccharomyces cerevisiae. Determination of enzymic activity confirmed the identity of the gene products as IPP isomerases. The corresponding gene was isolated from the genomic library of S. cerevisiae based on its nucleotide sequence, and was confirmed to have the same effect as the above two IPP isomerase genes when introduced into the E. coli transformant accumulating β-carotene. In the three E. coli strains carrying the individual exogenous IPP isomerase genes, the increases in carotenoid levels are comparable to the increases in IPP isomerase enzyme activity with reference to control strains possessing the endogenous gene alone. These results imply that IPP isomerase forms an influential step in isoprenoid biosynthesis of the prokaryote E. coli, with potential for the efficient production of industrially useful isoprenoids by metabolic engineering.

scholarly journals Haematococcus pluvialis Carotenoids Enrich Fractions Ameliorate Liver Fibrosis Induced by Thioacetamide in Rats: Modulation of Metalloproteinase and Its Inhibitor

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Farouk K. El-Baz ◽  
Abeer Salama ◽  
Sami I. Ali ◽  
Rania Elgohary
Keyword(s):  
Liver Fibrosis ◽  
Hepatic Fibrosis ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Haematococcus Pluvialis ◽  
Smooth Muscle Actin ◽  
Alpha Smooth Muscle Actin ◽  
Proinflammatory Mediators ◽  
Growth Factor Beta ◽  
Β Carotene

Hepatic fibrosis is a consequence of chronic liver diseases. Metalloproteinase and its inhibitor have crucial roles in the resolution of liver fibrosis. The current relevant study is aimed to evaluate the therapeutic effect of Haematococcus pluvialis (H. pluvialis) extract, astaxanthin-rich fraction, astaxanthin ester-rich fraction, and β-carotene-rich fraction as well as their mechanisms of action in curing hepatic fibrosis induced by thioacetamide (TAA). Liver fibrosis was induced using TAA (intraperitoneal injection, two times a week for 6 weeks), in a rat model and H. pluvialis extract (200 mg/kg), and other fractions (30 mg/kg) were orally administered daily for 4 weeks after the last TAA injection. Based on HPLC analysis, H. pluvialis extract contains β-carotene (12.95 mg/g, extract) and free astaxanthin (10.85 mg/g, extract), while HPLC/ESI-MS analysis revealed that H. pluvialis extract contains 28 carotenoid compounds including three isomers of free astaxanthin, α or β-carotene, lutein, 14 astaxanthin mono-esters, 5 astaxanthin di-esters, and other carotenoids. H. pluvialis and its fractions reduced liver enzymes, nitric oxide, collagen 1, alpha-smooth muscle actin, and transforming growth factor-beta as well as elevated catalase antioxidant activity compared to the TAA group. Also, H. pluvialis extract and its fractions exceedingly controlled the balance between metalloproteinase and its inhibitor, activated Kupffer cells proliferation, and suppressed liver apoptosis, necrobiosis, and fibrosis. These findings conclude that H. pluvialis extract and its fractions have an antifibrotic effect against TAA-induced liver fibrosis by regulating the oxidative stress and proinflammatory mediators, suppressing multiple profibrogenic factors, and modulating the metalloproteinase and its inhibitor pathway, recommending H. pluvialis extract and its fractions for the development of new effective medicine for treating hepatic fibrosis disorders.

scholarly journals The pigment composition of Haematococcus pluvialis microalgae cells upon cultivated on biogenic-deficient media

2020 ◽  
Vol 65 (2) ◽  
pp. 163-170
Author(s):  
N. V. Shalygo
Keyword(s):  
Chlorophyll A ◽  
Nutrient Medium ◽  
Haematococcus Pluvialis ◽  
Phosphorus Deficiency ◽  
Nitrogen Deficiency ◽  
Control Level ◽  
Pigment Composition ◽  
Nutrient Media ◽  
P Deficiency ◽  
Β Carotene

The accumulation of carotenoids (carotenogenesis) in the cells of Haematococcus pluvialis (strain IBCE H-17) upon cultivation in nutrient media deficient in nutrient elements (N and K + P) is shown. K and P deficiency in nutrient medium led to accumulation of astaxanthin, β-carotene, anteraxanthin, zeaxanthin and violaxanthin after 18 days of cultivation, while the amount of neoxanthin and lutein was at the control level. Carotenogenesis under such conditions was accompanied by high levels of chlorophyll (a + b). The use of nutrient medium deficient in N led to the accumulation of astaxanthin and, to a lesser extent, β-carotene in haematococcus cells, while lutein, anteraxanthin, zeaxanthin, violaxanthin and neoxanthin levels were lower or the same as control. N deficiency caused the decrease in amount of chlorophyll (a + b). Nitrogen deficiency was 4.3 times more effective than control medium and 1.8 times more effective than combined potassium and phosphorus deficiency in promoting astaxanthin accumulation.

scholarly journals Microalgae-Derived Pigments: A 10-Year Bibliometric Review and Industry and Market Trend Analysis

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3406 ◽  
Author(s):  
Samara C. Silva ◽  
Isabel C. F. R. Ferreira ◽  
Madalena M. Dias ◽  
M. Filomena Barreiro
Keyword(s):  
Spirulina Platensis ◽  
Dunaliella Salina ◽  
Haematococcus Pluvialis ◽  
Market Analysis ◽  
Microalgae Cultivation ◽  
Natural Pigments ◽  
Pigment Extraction ◽  
Bibliometric Review ◽  
Synthetic Pigments ◽  
Β Carotene

Microalgae productive chains are gaining importance as sustainable alternatives to obtain natural pigments. This work presents a review on the most promising pigments and microalgal sources by gathering trends from a 10-year bibliometric survey, a patents search, and an industrial and market analysis built from available market reports, projects and companies’ webpages. The performed analysis pointed out chlorophylls, phycocyanin, astaxanthin, and β-carotene as the most relevant pigments, and Chlorella vulgaris, Spirulina platensis, Haematococcus pluvialis, and Dunaliella salina, respectively, as the most studied sources. Haematococcus is referred in the highest number of patents, corroborating a high technological interest in this microalga. The biorefinery concept, investment in projects and companies related to microalgae cultivation and/or pigment extraction is increasingly growing, particularly, for phycocyanin from Spirulina platensis. These pieces of evidence are a step forward to consolidate the microalgal pigments market, which is expected to grow in the coming years, increasing the prospects of replacing synthetic pigments by natural counterparts.

scholarly journals Cloning and identification of a novel β-carotene hydroxylase gene from Haematococcus pluvialis and its function in Escherichia coli

2021 ◽  
Vol 55 ◽  
pp. 102245
Author(s):  
Danqiong Huang ◽  
Wenfu Liu ◽  
Anguo Li ◽  
Zhangli Hu ◽  
Jiangxin Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Haematococcus Pluvialis ◽  
Hydroxylase Gene ◽  
Β Carotene
Sign in / Sign up

Export Citation Format

Share Document