Deletion of the aroK gene is essential for high shikimic acid accumulation through the shikimate pathway in E. coli

2012 ◽  
Vol 119 ◽  
pp. 141-147 ◽  
Author(s):  
Kai Chen ◽  
Jie Dou ◽  
Shirui Tang ◽  
Yishun Yang ◽  
Hui Wang ◽  
...  
Keyword(s):  
Shikimic Acid ◽  
Shikimate Pathway ◽  
E Coli ◽  
Acid Accumulation

Effects of Glyphosate on Shikimic Acid Accumulation in Tobacco Cell Cultures with Low and High Yields of Cinnamoyl Putrescines

1981 ◽  
Vol 36 (3-4) ◽  
pp. 210-214 ◽  
Author(s):  
Jochen Berlin ◽  
Ludger Witte
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Cell Cultures ◽  
Phenylalanine Ammonia Lyase ◽  
Shikimic Acid ◽  
Shikimate Pathway ◽  
Tobacco Cell ◽  
Substrate Supply ◽  
Acid Accumulation ◽  
High Yields

To study the flow of carbon through the shikimate pathway in tobacco cell cultures with low and high yields of cinnamoyl putrescines, the cell cultures were treated with glyphosate. In the presence of glyphosate the levels of free shikimic acid were increased more than 300-fold by both cell lines. Despite of a normally 10-fold higher level of cinnamoyl putrescines, the high yielding cell line accumulated only 25% more free shikimic acid than the low yielding cell line. This result together with earlier observations indicated that the increased formation of cinnamoyl putrescines was rather limited by the activity of phenylalanine ammonia lyase than by increased substrate supply caused by alterations in the shikimate pathway

Glyphosate Applied Preharvest Induces Shikimic Acid Accumulation in Hard Red Spring Wheat (Triticum aestivum)

2003 ◽  
Vol 51 (14) ◽  
pp. 4004-4007 ◽  
Author(s):  
Gail A. Bresnahan ◽  
Frank A. Manthey ◽  
Kirk A. Howatt ◽  
Monisha Chakraborty
Keyword(s):  
Triticum Aestivum ◽  
Spring Wheat ◽  
Shikimic Acid ◽  
Hard Red Spring Wheat ◽  
Acid Accumulation

scholarly journals Targeting the Shikimate Pathway in the Malaria Parasite Plasmodium falciparum

1999 ◽  
Vol 43 (1) ◽  
pp. 175-177 ◽  
Author(s):  
Glenn A. McConkey
Keyword(s):  
Plasmodium Falciparum ◽  
Malaria Parasite ◽  
Shikimic Acid ◽  
Shikimate Pathway ◽  
Aminobenzoic Acid ◽  
Parasite Plasmodium ◽  
Parasite Plasmodium Falciparum

ABSTRACT The shikimate pathway presents an attractive target for malaria chemotherapy. Three shikimic acid analogs exhibited different effects on Plasmodium falciparum growth. (6R)-6-Fluoro-shikimate and (6S)-6-fluoro-shikimate inhibited growth (50% inhibitory concentrations, 1.5 × 10−5 and 2.7 × 10−4 M, respectively), whereas 2-fluoro-shikimate had no effect. para-Aminobenzoic acid abrogated the inhibition, demonstrating that the shikimate pathway was specifically targeted.

The shikimate pathway. Part 8. Synthesis of (−)-3(R)-amino-4(R),5(R)-dihydroxy-1-cyclohexene-1-carboxylic acid: The 3(R)-amino analogue of (−)-shikimic acid

Tetrahedron ◽  
1996 ◽  
Vol 52 (25) ◽  
pp. 8565-8580 ◽  
Author(s):  
Harry Adams ◽  
Neil A. Bailey ◽  
Roger Brettle ◽  
Richard Cross ◽  
Martyn Frederickson ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Shikimic Acid ◽  
Shikimate Pathway

Detection of Sourgrass (Digitaria insularis) Biotypes Resistant to Glyphosate in Brazil

Weed Science ◽  
2011 ◽  
Vol 59 (2) ◽  
pp. 171-176 ◽  
Author(s):  
Leonardo Bianco de Carvalho ◽  
Hugo Cruz-Hipolito ◽  
Fidel González-Torralva ◽  
Pedro Luis da Costa Aguiar Alves ◽  
Pedro Jacob Christoffoleti ◽  
...  
Keyword(s):  
Contact Angle ◽  
Dose Response ◽  
Shikimic Acid ◽  
Glyphosate Resistance ◽  
Resistance Factor ◽  
Natural Area ◽  
Perennial Weed ◽  
Perennial Crops ◽  
Spray Droplets ◽  
Acid Accumulation

Sourgrass is a perennial weed infesting annual and perennial crops in Brazil. Three biotypes (R1, R2, and R3) of sourgrass suspected to be glyphosate-resistant (R) and another one (S) from a natural area without glyphosate application, in Brazil, were tested for resistance to glyphosate based on screening, dose-response, and shikimic acid assays. Both screening and dose-response assays confirmed glyphosate resistance in the three sourgrass biotypes. Dose-response assay indicated a resistance factor of 2.3 for biotype R1 and 3.9 for biotypes R2 and R3. The hypothesis of a glyphosate resistance was corroborated on the basis of shikimic acid accumulation, where the S biotype accumulated 3.3, 5.0, and 5.7 times more shikimic acid than biotypes R1, R2, and R3, respectively, 168 h after treatment with 157.50 g ae ha−1of glyphosate. There were no differences in contact angle of spray droplets on leaves and spray retention, indicating that differential capture of herbicide by leaves was not responsible for resistance in these biotypes. The results confirmed resistance of sourgrass to glyphosate in Brazil.

The Role of the ydiB Gene, Which Encodes Quinate/Shikimate Dehydrogenase, in the Production of Quinic, Dehydroshikimic and Shikimic Acids in a PTS- Strain of Escherichia coli

2016 ◽  
Vol 27 (1) ◽  
pp. 11-21 ◽  
Author(s):  
Sofía García ◽  
Noemí Flores ◽  
Ramón De Anda ◽  
Georgina Hernández ◽  
Guillermo Gosset ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shikimic Acid ◽  
Sharp Decrease ◽  
Quinic Acid ◽  
Shikimate Dehydrogenase ◽  
Molar Yield ◽  
E Coli ◽  
Downstream Processes ◽  
Precise Role

The culture of engineered <i>Escherichia coli</i> for shikimic acid (SA) production results in the synthesis of quinic acid (QA) and dehydroshikimic acid (DHS), reducing SA yield and impairing downstream processes. The synthesis of QA by quinate/shikimate dehydrogenase (YdiB, <i>ydiB</i>) has been previously proposed; however, the precise role for this enzyme in the production of QA in engineered strains of <i>E. coli</i> for SA production remains unclear. We report the effect of the inactivation or the overexpression of <i>ydiB</i> in <i>E. coli</i> strain PB12.SA22 on SA, QA, and DHS production in batch fermentor cultures. The results showed that the inactivation of <i>ydiB </i>resulted in a 75% decrease in the molar yield of QA and a 6.17% reduction in the yield of QA (mol/mol) relative to SA with respect to the parental strain. The overexpression of <i>ydiB</i> caused a 500% increase in the molar yield of QA and resulted in a 152% increase in QA (mol/mol) relative to SA, with a sharp decrease in SA production. Production of SA, QA, and DHS in parental and derivative <i>ydiB </i>strains suggests that the synthesis of QA results from the reduction of 3-dehydroquinate by YdiB before its conversion to DHS.

Recombinant expression of glpK and glpD genes improves the accumulation of shikimic acid in E. coli grown on glycerol

2014 ◽  
Vol 30 (12) ◽  
pp. 3263-3272 ◽  
Author(s):  
Yang Yang ◽  
Chao Yuan ◽  
Jie Dou ◽  
Xiaorong Han ◽  
Hui Wang ◽  
...  
Keyword(s):  
Shikimic Acid ◽  
Recombinant Expression ◽  
E Coli

Effects of pre-harvest glyphosate use on protein composition and shikimic acid accumulation in spring wheat

2020 ◽  
Vol 332 ◽  
pp. 127422 ◽  
Author(s):  
Maneka Malalgoda ◽  
Jae-Bom Ohm ◽  
Kirk A. Howatt ◽  
Andrew Green ◽  
Senay Simsek
Keyword(s):  
Spring Wheat ◽  
Shikimic Acid ◽  
Protein Composition ◽  
Acid Accumulation

scholarly journals INHIBITION OF ESCHERICHIA COLI BY p-AMINOBENZOIC ACID AND ITS REVERSAL BY p-HYDROXYBENZOIC ACID

1951 ◽  
Vol 94 (3) ◽  
pp. 243-254 ◽  
Author(s):  
Bernard D. Davis
Keyword(s):  
Shikimic Acid ◽  
Growth Inhibitor ◽  
Metabolic Effects ◽  
Hydroxybenzoic Acid ◽  
Aminobenzoic Acid ◽  
Chlorobenzoic Acid ◽  
E Coli ◽  
Nitrobenzoic Acid ◽  
Low Concentrations ◽  
High Concentrations

p-Aminobenzoic acid (PABA) exerts three metabolic effects on E. coli: it acts as a normal vitamin at low concentrations, as a source of another vitamin, p-hydroxybenzoic acid (POB), at moderate concentrations, and as a growth inhibitor at high concentrations (150 to 1600 µg./ml.). The inhibition is competitively reversed by POB in 1/100 the concentration of PABA. The inhibition is also reversed to a limited extent by shikimic acid and compound X, precursors of POB. p-Nitrobenzoic acid is an inhibitory competitor of both POB and PABA. The retardation of growth produced by PABA and other competitive analogues of POB (p-nitrobenzoic acid; 4,4'-dihydroxydiphenyl sulfone; phenosulfazole) is converted to complete bacteriostasis by the addition of L-aspartic acid in a remarkably low concentration (1 µg./ml.)) without change in the competitive ratio with POB. The mechanism underlying this synergism is not clear. In contrast to wild type, mutants that require POB not only are inhibited by much lower concentrations of the above analogues, but also show inhibition by weaker competitors of POB such as p-hydroxybenzenesulfonamide, p-chlorobenzoic acid, and p-fluorobenzoic acid.

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