Untreated wheat straw: Potential source for diverse cellulolytic enzyme secretion by Penicillium janthinellum EMS-UV-8 mutant

2015 ◽  
Vol 196 ◽  
pp. 518-524 ◽  
Author(s):  
Bhawna Sharma ◽  
Ruchi Agrawal ◽  
Reeta Rani Singhania ◽  
Alok Satlewal ◽  
Anshu Mathur ◽  
...  
RSC Advances ◽  
2014 ◽  
Vol 4 (84) ◽  
pp. 44726-44732 ◽  
Author(s):  
Mukund Adsul ◽  
Bhawna Sharma ◽  
Reeta Rani Singhania ◽  
Jitendra Kumar Saini ◽  
Ankita Sharma ◽  
...  

A prepared enzyme cocktail from different fungal enzyme preparations increases the hydrolysis of avicel/wheat straw by increasing synergism between the same or different types of cellulases.


AMB Express ◽  
2013 ◽  
Vol 3 (1) ◽  
pp. 65 ◽  
Author(s):  
Claudia Thompson ◽  
Walter Beys-da-Silva ◽  
Lucélia Santi ◽  
Markus Berger ◽  
Marilene Vainstein ◽  
...  

Author(s):  
Ehsan Abbasi Atibeh ◽  
Ahmet Yozgatligil

In this study an attempt was done to profoundly explore the pyrolysis and combustion behaviors and emission characteristics of lignite samples in O2/N2 and O2/CO2 (oxy-fuel conditions) ambients. A special focus was allocated to the effects of three inorganic materials, potassium (K), calcium (Ca) and iron (Fe) on combustion characteristics of Turkish lignite using non-isothermal Thermo-gravimetric Analysis (TGA) technique combined with Fourier Transform Infrared (FTIR) spectroscopy and the effects of ambient gases and various oxygen mole fractions were considered. Eventually the co-processing combustion tests of lignite and the ash contents of different biomass fuels were investigated and the possible way of using biomass as a potential source of inexpensive catalysts in combustion processes were discussed. Co-processing combustion tests of lignite and biomass ash contents indicated that the hazelnut shell and walnut shell ash contents were significantly effective in increasing the char reactivity of lignite due to high concentration of potassium based oxides during combustion tests carried out in both air and 30% O2 in CO2 ambients. Furthermore the catalytic reactivity of wheat straw and cattle manure ash contents were observed in the second region of combustion regarding volatile matter release and combustion in both air and 30% O2 in CO2 ambients. These results are thought to be due to high concentrations of Alkali and Alkaline earth metals existed in the impregnated lignite samples with wheat straw and cattle manure ash contents and especially Na-based oxides in the cattle manure form. Finally in the case of lignite sample impregnated with saw dust ash content, it was observed that the impregnated lignite was significantly more reactive in devolatalization process in 30% O2 in CO2 ambients. These results revealed that the ash contents of walnut and hazelnut shell biomass fuels can be used as a potential source of inexpensive K-based catalysts in the co-processing of coal and biomass ash. Furthermore high concentrations of Alkali and Alkaline earth metals existed in the ash contents of biomass fuels like wheat straw, cattle manure and saw dust can make them suitable sources of inexpensive catalysts and develop a step forward in economic aspects of catalytic coal combustion.


Author(s):  
O. M. Yurieva ◽  
A. P. Hryhanskyi ◽  
S. O. Syrchin ◽  
L. T. Nakonechna ◽  
A. K. Pavlychenko ◽  
...  

Aim. The aim of this research was a comparative study of β-glucosidase activity of endophytic and soil Penicillium funiculosum strains. Methods. β-Gucosidase activity was determined by hydrolysis of pNPG on 4th and 6th days of cultivation. Barcoding of P. funiculosum 16795 DNA was carried out with ITS1 and ITS4 primers. Results. Obtained data demonstrate the ability of endophytic and soil P. funiculosum strains to synthesize β-glucosidase activity and hydrolyze Na-CMC and wheat straw. Studied activities enhanced with increasing cultivation time of micromycetes. Endophytic isolates of P. funiculosum had higher β-glucosidase activities than soil ones. Conclusions. P. funiculosum strains, especially 16795, are promising for further research of β-glucosidase preparations as additional component to the T. reesei enzymes, as well as to create recombinant constructs for obtaining a balanced composition of cellulolytic enzyme complexes.Keywords: Penicillium funiculosum, β-glucosidase activity, endophytes, soil strains.


2014 ◽  
Vol 169 ◽  
pp. 490-495 ◽  
Author(s):  
Reeta Rani Singhania ◽  
Jitendra Kumar Saini ◽  
Reetu Saini ◽  
Mukund Adsul ◽  
Anshu Mathur ◽  
...  

Author(s):  
Philippe Fragu

The identification, localization and quantification of intracellular chemical elements is an area of scientific endeavour which has not ceased to develop over the past 30 years. Secondary Ion Mass Spectrometry (SIMS) microscopy is widely used for elemental localization problems in geochemistry, metallurgy and electronics. Although the first commercial instruments were available in 1968, biological applications have been gradual as investigators have systematically examined the potential source of artefacts inherent in the method and sought to develop strategies for the analysis of soft biological material with a lateral resolution equivalent to that of the light microscope. In 1992, the prospects offered by this technique are even more encouraging as prototypes of new ion probes appear capable of achieving the ultimate goal, namely the quantitative analysis of micron and submicron regions. The purpose of this review is to underline the requirements for biomedical applications of SIMS microscopy.Sample preparation methodology should preserve both the structural and the chemical integrity of the tissue.


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