A quantitative evaluation of ethylene production in the recombinant cyanobacterium Synechocystis sp. PCC 6803 harboring the ethylene-forming enzyme by membrane inlet mass spectrometry

2016 ◽  
Vol 202 ◽  
pp. 142-151 ◽  
Author(s):  
Tomáš Zavřel ◽  
Henning Knoop ◽  
Ralf Steuer ◽  
Patrik R. Jones ◽  
Jan Červený ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Evaluation ◽  
Ethylene Production ◽  
Membrane Inlet Mass Spectrometry ◽  
Synechocystis Sp ◽  
Pcc 6803

The effect of nitrogen starvation on membrane lipids of Synechocystis sp. PCC 6803 investigated by using easy ambient sonic-spray ionization mass spectrometry

2019 ◽  
Vol 1861 (10) ◽  
pp. 183027 ◽  
Author(s):  
Leonidas Mavroudakis ◽  
Eleftheria-Angeliki Valsami ◽  
Sofia Grafanaki ◽  
Theofania-Pagona Andreadaki ◽  
Demetrios F. Ghanotakis ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nitrogen Starvation ◽  
Membrane Lipids ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Sonic Spray Ionization ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Ethylene production with engineered Synechocystis sp PCC 6803 strains

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Vinod Puthan Veetil ◽  
S. Andreas Angermayr ◽  
Klaas J. Hellingwerf
Keyword(s):  
Ethylene Production ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Comparative Proteomic Profiling of Marine and Freshwater Synechocystis Strains Using Liquid Chromatography-Tandem Mass Spectrometry

2020 ◽  
Vol 8 (10) ◽  
pp. 790
Author(s):  
Dami Kwon ◽  
Jong-Moon Park ◽  
Van-An Duong ◽  
Seong-Joo Hong ◽  
Byung-Kwan Cho ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Proteomic Profiling ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Acquisition Method ◽  
Synechocystis Sp ◽  
Pcc 6803

Freshwater Synechocystis sp. PCC 6803 has been considered to be a platform for the production of the next generation of biofuels and is used as a model organism in various fields. Various genomics, transcriptomics, metabolomics, and proteomics studies have been performed on this strain, whereas marine Synechocystis sp. PCC 7338 has not been widely studied despite its wide distribution. This study analyzed the proteome profiles of two Synechocystis strains using a liquid chromatography–tandem mass spectrometry-based bottom-up proteomic approach. Proteomic profiling of Synechocystis sp. PCC 7338 was performed for the first time with a data-dependent acquisition method, revealing 18,779 unique peptides and 1794 protein groups. A data-independent acquisition method was carried out for the comparative quantitation of Synechocystis sp. PCC 6803 and 7338. Among 2049 quantified proteins, 185 up- and 211 down-regulated proteins were defined in Synechocystis sp. PCC 7338. Some characteristics in the proteome of Synechocystis sp. PCC 7338 were revealed, such as its adaptation to living conditions, including the down-regulation of some photosynthesis proteins, the up-regulation of kdpB, and the use of osmolyte glycine as a substrate in C1 metabolism for the regulation of carbon flow. This study will facilitate further studies on Synechocystis 7338 to define in depth the proteomic differences between it and other Synechocystis strains.

scholarly journals Ethylene production in Synechocystis sp. PCC 6803 promotes phototactic movement

Microbiology ◽  
2017 ◽  
Vol 163 (12) ◽  
pp. 1937-1945 ◽  
Author(s):  
Ekaterina Kuchmina ◽  
Stephan Klähn ◽  
Annik Jakob ◽  
Werner Bigott ◽  
Heike Enke ◽  
...  
Keyword(s):  
Ethylene Production ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Biosynthesis of arsenolipids by the cyanobacterium Synechocystis sp. PCC 6803

2014 ◽  
Vol 11 (5) ◽  
pp. 506 ◽  
Author(s):  
Xi-Mei Xue ◽  
Georg Raber ◽  
Simon Foster ◽  
Song-Can Chen ◽  
Kevin A. Francesconi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mutant Strain ◽  
High Performance ◽  
Tandem Mass ◽  
Wild Type ◽  
Arsenic Compounds ◽  
Synechocystis Sp ◽  
Pcc 6803

Environmental context Arsenic biotransformation processes play a key role in the cycling of arsenic in aquatic systems. We show that a freshwater cyanobacterium can convert inorganic arsenic into arsenolipids, and the conversion efficiency depends on the arsenic concentration. The role of these novel arsenic compounds remains to be elucidated. Abstract Although methylated arsenic and arsenosugars have been verified in various freshwater organisms, lipid-soluble arsenic compounds have not been identified. Here, we report investigations with the model organism cyanobacterium Synechocystis sp. PCC 6803 wild type and ΔarsM (arsenic(III) S-adenosylmethionine methyltransferase) mutant strain, which lacks the enzymes for arsenic methylation cultured in various concentrations of arsenate (AsV). Although Synechocystis accumulated higher arsenic concentrations at the higher exposure levels, the bioaccumulation factor decreased with increasing AsV. The accumulated arsenic in the cells was partitioned into water-soluble and lipid-soluble fractions; lipid-soluble arsenic was found in Synechocystis wild type cells (3–35% of the total depending on the level of arsenic exposure), but was not detected in Synechocystis ΔarsM mutant strain showing that ArsM was required for arsenolipid biosynthesis. The arsenolipids present in Synechocystis sp. PCC 6803 were analysed by high performance liquid chromatography–inductively coupled plasma–mass spectrometry, high performance liquid chromatography–electrospray mass spectrometry, and high resolution tandem mass spectrometry. The two major arsenolipids were characterised as arsenosugar phospholipids based on their assigned molecular formulas C47H88O14AsP and C47H90O14AsP, and tandem mass spectrometric data demonstrated the presence of the phosphate arsenosugar and acylated glycerol groups.

scholarly journals Enhancing photosynthetic production of ethylene in genetically engineered Synechocystis sp. PCC 6803

2015 ◽  
Vol 17 (1) ◽  
pp. 421-434 ◽  
Author(s):  
Tao Zhu ◽  
Xiaoman Xie ◽  
Zhimin Li ◽  
Xiaoming Tan ◽  
Xuefeng Lu
Keyword(s):  
Ethylene Production ◽  
Genetically Engineered ◽  
Genetic Modifications ◽  
Photosynthetic Production ◽  
Synechocystis Sp ◽  
Pcc 6803

The enhanced ethylene production (9.7 mL L−1h−1) was achieved by genetic modifications and improved cultivation ofSynechocystissp. PCC 6803.

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