A biotechnological process efficiently co-produces two high value-added products, glucose and xylooligosaccharides, from sugarcane bagasse

Lignocellulosic residues obtained from crops cultivation form useful sources to be used as substrate for bioconversion processes. Sugarcane bagasse, which is a complex substrate obtained from the processing of sugar cane, is an important biomass among such sources. Due to its abundant availability, it can serve as an ideal substrate for microbial processes for the production of value added products. This paper reviews recent developments on biological processes developed on production of various products in solid state fermentation using sugarcane bagasse as the substrate and describes production of protein enriched feed, enzymes, amino acid, organic acids and compounds of pharmaceutical importance, etc. through microbial means.

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Bioconversion of cassava bagasse and sugarcane bagasse using cheap home-made enzymatic cocktails

10.21203/rs.3.rs-605152/v1 ◽

2021 ◽

Author(s):

Gabriela Verónica Díaz ◽

Silvana Soledad Sawostjanik Afanasiuk ◽

Romina Olga Coniglio ◽

Juan Ernesto Velázquez ◽

Pedro Darío Zapata ◽

...

Keyword(s):

Sugarcane Bagasse ◽

Value Added ◽

Added Value ◽

Cassava Bagasse ◽

Commercial Enzymes ◽

Before And After ◽

Agricultural Industries ◽

The Cost ◽

Agroindustrial Wastes ◽

Value Added Products

Abstract The agricultural industries generate lignocellulosic wastes that can be modified by fungi to generate high value-added products. The aim of this work was to analyze the efficiency of the bioconversion of sugarcane bagasse and cassava bagasse using two cheap home-made enzymatic cocktails from Aspergillus niger LBM 134 (produced also from agroindustrial wastes) and compare the hydrolysis yield with that obtained from the bioconversion using commercial enzymes. Sugarcane bagasse and cassava bagasse were pretreated with a soft alkaline solution before the hydrolysis carried out with home-made enzymatic cocktails of A. niger LBM 134 and with commercial enzymes to compare their performances. Mono and polysaccharides were analyzed before and after the bioconversion of both bagasses as well as their microscopic structure. The maximal yield was the 80% of total glucans saccharified from cassava bagasse. The bioconversion of both bagasses were better when we used the home-made enzymatic cocktails than commercial enzymes. We obtained high added-value products from agroindustrial wastes, home-made enzymatic cocktails and hydrolysates rich in fermentable sugars. The importance of this work lays in the higher performance of the cheap home-made enzymatic cocktails over the hydrolytic performance of commercial enzymes due to the cost of producing the home-made enzymatic cocktails were more than 500 times lower than commercial enzymes.

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Cellulosic value-added products from sugarcane bagasse

Cellulose ◽

10.1007/s10570-021-03918-3 ◽

2021 ◽

Author(s):

Selorm Torgbo ◽

Vo Minh Quan ◽

Prakit Sukyai

Keyword(s):

Sugarcane Bagasse ◽

Value Added ◽

Value Added Products

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COMPARISON OF ETHANOL YIELD BETWEEN SEPARATE AND SIMULTANEOUS HYDROLYSIS AND ETHANOL FERMENTATION OF FORMIC- FRACTIONATED SUGARCANE BAGASSE

Vietnam Journal of Science and Technology ◽

10.15625/2525-2518/54/2a/11934 ◽

2018 ◽

Vol 54 (2A) ◽

pp. 222

Author(s):

Ngo Duy Sa

Keyword(s):

Sugarcane Bagasse ◽

Ethanol Fermentation ◽

Ethanol Yield ◽

Food Additives ◽

Value Added ◽

Significant Inhibition ◽

Process Time ◽

Separate Hydrolysis And Fermentation ◽

High Ethanol ◽

Value Added Products

The fractionation of sugarcane bagasse using formic acid allowed removing lignin and hemicellulose, obtaining a material containing up to 90 % cellulose. The material can be easily hydrolyzed into glucose to serve as materials to produce high value added products such as biofuel, chemicals, pharmaceuticals, food additives, and the likes. The hydrolysate of fractionated bagasse was easily fermented with a (ethanol) fermentation yield attained 91.08 ± 2.02 %, showing no significant inhibition to the yeast in the hydrolysate. In this study, a process of simultaneous hydrolysis and fermentation (SSF) was performed to convert fractionated sugarcane bagasse at 20 % consistency to ethanol. The process with 6h pre-hydrolysis at 50 0C then SSF at 37 0C could attain a high ethanol concentration of 82.46 ± 3.42 g/L in the fermentation with the ethanol recovery yield of 81.66±1.88%; which was15.37 ± 1.06 % higher than that of the separate hydrolysis and fermentation (SHF) process (70.78 ± 0.25 %). In addition, in the SSF, the process time was shorten to 4 days instead of 7 days in the SHF.

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Bioconversion of Sugarcane Bagasse into Value-Added Products by Bioaugmentation of Endogenous Cellulolytic and Fermentative Communities

Waste and Biomass Valorization ◽

10.1007/s12649-018-0201-5 ◽

2018 ◽

Vol 10 (7) ◽

pp. 1899-1912 ◽

Cited By ~ 3

Author(s):

Laís Américo Soares ◽

Juliana Kawanish Braga ◽

Fabrício Motteran ◽

Isabel Kimiko Sakamoto ◽

Patrícia Aparecida Santiago Monteiro ◽

...

Keyword(s):

Sugarcane Bagasse ◽

Value Added ◽

Value Added Products

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OPPORTUNITIES FOR STRATEGIC DEVELOPMENT OF BIO-ECONOMY IN THE FOOD AND TOURISM INDUSTRY

Knowledge International Journal ◽

10.35120/kij28051681g ◽

2018 ◽

Vol 28 (5) ◽

pp. 1681-1684

Author(s):

Georgi Toskov ◽

Ana Yaneva ◽

Stanko Stankov ◽

Hafize Fidan

Keyword(s):

National Level ◽

Value Added ◽

Tourism Industry ◽

Small Companies ◽

Food Sector ◽

Strategic Development ◽

Innovation Potential ◽

Wide Range ◽

Foreign Brands ◽

Value Added Products

The European Commission defines the bioeconomy as "the production of renewable biological resources and the conversion of these resources and waste streams into value added products, such as food, feed, bio-based products and bioenergy. Its sectors and industries have strong innovation potential due to their use of a wide range of sciences, enabling and industrial technologies, along with local and implied knowledge." The Bulgarian food industry faces a lot of challenges on the local and national level, which have direct influence on the structure of the production companies. Most of the enterprises from the food sector produce under foreign brands in order to be flexible partners to the large Bulgarian retail chains. The small companies from the food sector are not able to develop as an independent competitive producer on the territory of their local markets. This kind of companies rarely has a working strategy for positioning on new markets. In order to consolidate their already built positions for long period of time, the producers are trying to optimize their operations in a short term. However, the unclear vision of the companies for the business segment does not allow them to fully develop. Tourism in Bulgaria is a significant contributor to the country's economy.

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Oxidized indium with transformable dimensions for CO2 electroreduction toward formate aided by oxygen vacancies

Sustainable Energy & Fuels ◽

10.1039/d0se00498g ◽

2020 ◽

Vol 4 (7) ◽

pp. 3726-3731

Author(s):

Fenghui Ye ◽

Jinghui Gao ◽

Yilin Chen ◽

Yunming Fang

Keyword(s):

Crucial Role ◽

Oxygen Vacancies ◽

Value Added ◽

Promising Technique ◽

Co2 Electroreduction ◽

Value Added Products

Electroreduction of CO2 into value-added products is a promising technique in which the structure of the catalyst plays a crucial role.

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β-Galactosidases from a Sequence-Based Metagenome: Cloning, Expression, Purification and Characterization

Microorganisms ◽

10.3390/microorganisms9010055 ◽

2020 ◽

Vol 9 (1) ◽

pp. 55

Author(s):

María Florencia Eberhardt ◽

José Matías Irazoqui ◽

Ariel Fernando Amadio

Keyword(s):

Bacterial Species ◽

Value Added ◽

Raw Material ◽

Activity Levels ◽

Treatment Technology ◽

Catalytic Domains ◽

Stabilization Ponds ◽

Cazy Database ◽

Genes Encoding ◽

Value Added Products

Stabilization ponds are a common treatment technology for wastewater generated by dairy industries. Large proportions of cheese whey are thrown into these ponds, creating an environmental problem because of the large volume produced and the high biological and chemical oxygen demands. Due to its composition, mainly lactose and proteins, it can be considered as a raw material for value-added products, through physicochemical or enzymatic treatments. β-Galactosidases (EC 3.2.1.23) are lactose modifying enzymes that can transform lactose in free monomers, glucose and galactose, or galactooligosacharides. Here, the identification of novel genes encoding β-galactosidases, identified via whole-genome shotgun sequencing of the metagenome of dairy industries stabilization ponds is reported. The genes were selected based on the conservation of catalytic domains, comparing against the CAZy database, and focusing on families with β-galactosidases activity (GH1, GH2 and GH42). A total of 394 candidate genes were found, all belonging to bacterial species. From these candidates, 12 were selected to be cloned and expressed. A total of six enzymes were expressed, and five cleaved efficiently ortho-nitrophenyl-β-galactoside and lactose. The activity levels of one of these novel β-galactosidase was higher than other enzymes reported from functional metagenomics screening and higher than the only enzyme reported from sequence-based metagenomics. A group of novel mesophilic β-galactosidases from diary stabilization ponds’ metagenomes was successfully identified, cloned and expressed. These novel enzymes provide alternatives for the production of value-added products from dairy industries’ by-products.

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Milk Fat Globule Membrane Proteome and Micronutrients in the Milk Lipid Fraction: Insights into Milk Bioactive Compounds

Dairy ◽

10.3390/dairy2020018 ◽

2021 ◽

Vol 2 (2) ◽

pp. 202-217

Author(s):

Michele Manoni ◽

Donata Cattaneo ◽

Sharon Mazzoleni ◽

Carlotta Giromini ◽

Antonella Baldi ◽

...

Keyword(s):

Milk Fat ◽

Lipid Fraction ◽

Value Added ◽

Milk Lipid ◽

Milk Fat Globule Membrane ◽

Fat Globules ◽

Milk Fat Globules ◽

Milk Fat Globule ◽

Fat Globule ◽

Value Added Products

Milk lipids are composed of milk fat globules (MFGs) surrounded by the milk fat globule membrane (MFGM). MFGM protects MFGs from coalescence and enzymatic degradation. The milk lipid fraction is a “natural solvent” for macronutrients such as phospholipids, proteins and cholesterol, and micronutrients such as minerals and vitamins. The research focused largely on the polar lipids of MFGM, given their wide bioactive properties. In this review we discussed (i) the composition of MFGM proteome and its variations among species and phases of lactation and (ii) the micronutrient content of human and cow’s milk lipid fraction. The major MFGM proteins are shared among species, but the molecular function and protein expression of MFGM proteins vary among species and phases of lactation. The main minerals in the milk lipid fraction are iron, zinc, copper and calcium, whereas the major vitamins are vitamin A, β-carotene, riboflavin and α-tocopherol. The update and the combination of this knowledge could lead to the exploitation of the MFGM proteome and the milk lipid fraction at nutritional, biological or technological levels. An example is the design of innovative and value-added products, such as MFGM-supplemented infant formulas.

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Extraction of sugarcane bagasse arabinoxylan, integrated with enzymatic production of xylo-oligosaccharides and separation of cellulose

Biotechnology for Biofuels ◽

10.1186/s13068-021-01993-z ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Leila Khaleghipour ◽

Javier A. Linares-Pastén ◽

Hamid Rashedi ◽

Seyed Omid Ranaei Siadat ◽

Andrius Jasilionis ◽

...

Keyword(s):

Sugarcane Bagasse ◽

Sugar Content ◽

Value Added ◽

Apparent Molecular Weight ◽

Bacillus Halodurans ◽

Glycoside Hydrolase Family ◽

High Concentration ◽

Enzymatic Production ◽

Successful Separation ◽

Ft Ir

AbstractSugarcane processing roughly generates 54 million tonnes sugarcane bagasse (SCB)/year, making SCB an important material for upgrading to value-added molecules. In this study, an integrated scheme was developed for separating xylan, lignin and cellulose, followed by production of xylo-oligosaccharides (XOS) from SCB. Xylan extraction conditions were screened in: (1) single extractions in NaOH (0.25, 0.5, or 1 M), 121 °C (1 bar), 30 and 60 min; (2) 3 × repeated extraction cycles in NaOH (1 or 2 M), 121 °C (1 bar), 30 and 60 min or (3) pressurized liquid extractions (PLE), 100 bar, at low alkalinity (0–0.1 M NaOH) in the time and temperature range 10–30 min and 50–150 °C. Higher concentration of alkali (2 M NaOH) increased the xylan yield and resulted in higher apparent molecular weight of the xylan polymer (212 kDa using 1 and 2 M NaOH, vs 47 kDa using 0.5 M NaOH), but decreased the substituent sugar content. Repeated extraction at 2 M NaOH, 121 °C, 60 min solubilized both xylan (85.6% of the SCB xylan), and lignin (84.1% of the lignin), and left cellulose of high purity (95.8%) in the residuals. Solubilized xylan was separated from lignin by precipitation, and a polymer with β-1,4-linked xylose backbone substituted by arabinose and glucuronic acids was confirmed by FT-IR and monosaccharide analysis. XOS yield in subsequent hydrolysis by endo-xylanases (from glycoside hydrolase family 10 or 11) was dependent on extraction conditions, and was highest using xylan extracted by 0.5 M NaOH, (42.3%, using Xyn10A from Bacillus halodurans), with xylobiose and xylotriose as main products. The present study shows successful separation of SCB xylan, lignin, and cellulose. High concentration of alkali, resulted in xylan with lower degree of substitution (especially reduced arabinosylation), while high pressure (using PLE), released more lignin than xylan. Enzymatic hydrolysis was more efficient using xylan extracted at lower alkaline strength and less efficient using xylan obtained by PLE and 2 M NaOH, which may be a consequence of polymer aggregation, via remaining lignin interactions.

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