Accelerated bio-methane production rate in thermophilic digestion of cardboard with appropriate biochar: Dose-response kinetic assays, hybrid synergistic mechanism, and microbial networks analysis

2019 ◽  
Vol 290 ◽  
pp. 121782 ◽  
Author(s):  
Dunjie Li ◽  
Liuying Song ◽  
Hongli Fang ◽  
Ping Li ◽  
Yue Teng ◽  
...  
1997 ◽  
Vol 35 (8) ◽  
pp. 209-215 ◽  
Author(s):  
Shuzo Tanaka ◽  
Toshio Kobayashi ◽  
Ken-ichi Kamiyama ◽  
Ma. Lolita N. Signey Bildan

Effects of pretreatment on the anaerobic digestion of waste activated sludge (WAS) were investigated in terms of VSS solubilization and methane production by batch experiments. The methods of pretreatment studied are NaOH addition (chemical), heating (thermal) and heating with NaOH addition (thermochemical) to the domestic WAS and to the combined WAS from domestic, commercial and industrial wastewaters. The thermochemical pretreatment gave the best result among three methods in the combined WAS, i.e., the VSS was solubilized by 40-50% and the methane production increased by more than 200% over the control when the WAS was heated at 130°C for 5 minutes with the dose 0.3 g NaOH/g VSS. In the domestic WAS, the VSS solubilization rate was 70-80% but the increase of the methane production was about 30% after thermochemically pretreated. The domestic WAS consists of 41% protein, 25% lipid and 14% carbohydrate on COD basis, and the solubilization rate of protein, which is the largest constituent of the WAS, was 63% in the thermochemical pretreatment. Although the effect of the thermochemical pretreatment on the methane production was higher to the combined WAS than to the domestic WAS, the methane production rate was 21.9 ml CH4/g VSSWAS·day in the domestic WAS and 12.8 ml CH4/g VSSWAS·day in the combined WAS.


Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 860 ◽  
Author(s):  
Helen Coarita Fernandez ◽  
Diana Amaya Ramirez ◽  
Ruben Teixeira Franco ◽  
Pierre Buffière ◽  
Rémy Bayard

Different methods were tested to evaluate the performance of a pretreatment before anaerobic digestion. Besides conventional biochemical parameters, such as the biochemical methane potential (BMP), the methane production rate, or the extent of solubilization of organic compounds, methods for physical characterization were also developed in the present work. Criteria, such as the particle size distribution, the water retention capacity, and the rheological properties, were thus measured. These methods were tested on samples taken in two full-scale digesters operating with cattle manure as a substrate and using hammer mills. The comparison of samples taken before and after the pretreatment unit showed no significant improvement in the methane potential. However, the methane production rate increased by 15% and 26% for the two hammer mills, respectively. A relevant improvement of the rheological properties was also observed. This feature is likely correlated with the average reduction in particle size during the pretreatment operation, but these results needs confirmation in a wider range of systems.


2006 ◽  
Vol 54 (9) ◽  
pp. 221-227 ◽  
Author(s):  
Y. Yoneyama ◽  
A. Nishii ◽  
M. Nishimoto ◽  
N. Yamada ◽  
T. Suzuki

Upflow anaerobic sludge blanket (UASB) methane fermentation treatment of cow manure that was subjected to screw pressing, thermal treatment and subsequent solid–liquid separation was studied. Conducting batch scale tests at temperatures between 140 and 180 °C, the optimal temperature for sludge settling and the color suppression was found to be between160–170 °C. UASB treatment was carried out with a supernatant obtained from the thermal treatment at the optimal conditions (170 °C for 30 minutes) and polymer-dosed solid–liquid separation. In the UASB treatment with a CODCr loading of 11.7 kg/m3/d and water temperature of 32.2 °C, the CODCr level dropped from 16,360 mg/L in raw water to 3,940 mg/L in treated water (CODCr removal rate of 75.9%), and the methane production rate per CODCr was 0.187 Nm3/kg. Using wastewater thermal-treated at the optimal conditions, also a methane fermentation treatment with a continuously stirred tank reactor (CSTR) was conducted (CODCr in raw water: 38,000 mg/L, hydraulic retention time (HRT): 20 days, 35 °C). At the CODCr loading of 1.9 kg/m3/d, the methane production rate per CODCr was 0.153 Nm3/kg. This result shows that UASB treatment using thermal pre-treatment provides a CODCr loading of four times or more and a methane production rate of 1.3 times higher than the CSTR treatment.


2018 ◽  
Vol 2 (1) ◽  
pp. 18-22
Author(s):  
Rafiqqah Mohamad Sabri ◽  

In this research, sago mill effluent was treated using anaerobic sequencing batch reactor (ASBR). Seven HRT from 10 to 1.5 days were tested to evaluate the methane production from sago mill effluent. The findings revealed the highest methane production rate was found at 1.288 L CH4/L reactor. d under HRT of 2 days The results showed that COD removals decreased from 70% to 47% as HRT was reduced from 10 to 2 days. The HRT 1.5 days was found critical for the studied system, which leads to decreased in methane production, yield and COD removal. Overall, ASBR was capable to treat sago mill effluent in producing methane by means of anaerobic digestion.


1999 ◽  
Vol 40 (8) ◽  
pp. 195-202 ◽  
Author(s):  
G. Gonzalez-Gil ◽  
R. Kleerebezem ◽  
A. van Aelst ◽  
G. R. Zoutberg ◽  
A. I. Versprille ◽  
...  

Methanogenesis from formaldehyde mainly occurred via intermediates as confirmed by the increased concentrations of methanol and H2 in the liquid and gas phases respectively during formaldehyde conversion. While formaldehyde was readily transformed, the methane production rate was immediately and strongly inhibited. Formaldehyde toxicity was in part reversible since the methane production rate recovered after formaldehyde depletion. This recovery can not be explained by biomass growth. The toxicity of formaldehyde was also in part irreversible, since the degree of recovery was not complete. This loss in the methane production rate likely can be attributed to biomass decay as suggested by its linear relation with the amount of formaldehyde dosed, as well as by scanning electron microscopy observations. The addition of the same amount of formaldehyde either in a slug or in a continuous mode caused the same loss in the methane production rate. Thus for the treatment of formaldehyde-containing streams, a balance between loss in the rate (formaldehyde-related decay) and bacterial growth should be attained. By combining good biomass retention and internal dilution of the wastewater, industrial streams containing formaldehyde can still be treated anaerobically.


Processes ◽  
2020 ◽  
Vol 8 (10) ◽  
pp. 1226
Author(s):  
Elvira E. Ziganshina ◽  
Dmitry E. Belostotskiy ◽  
Svetlana S. Bulynina ◽  
Ayrat M. Ziganshin

Anaerobic digestion is an important technology to receive energy from various types of biomass. In this work, the impact of granular activated carbon (GAC) on the mesophilic anaerobic co-digestion of sugar beet pulp and distillers grains was investigated. After a short period, anaerobic reactors began to produce biomethane and were ready for completion within 19–24 days. The addition of GAC to reactors (5–10 g L−1) significantly enhanced the methane production rate and consumption of produced volatile fatty acids. Thus, the maximum methane production rate increased by 13.7% in the presence of GAC (5 g L−1). Bacterial and archaeal community structure and dynamics were investigated, based on 16S rRNA genes analysis. The abundant classes of bacteria in GAC-free and GAC-containing reactors were Clostridia, Bacteroidia, Actinobacteria, and Synergistia. Methanogenic communities were mainly represented by the genera Methanosarcina, Methanoculleus, Methanothrix, and Methanomassiliicoccus in GAC-free and GAC-containing reactors. Our results indicate that the addition of granular activated carbon at appropriate dosages has a positive effect on anaerobic co-digestion of by-products of the processing of sugar beet and ethanol distillation process.


2019 ◽  
Vol 20 (18) ◽  
pp. 4415 ◽  
Author(s):  
Anna Szafranek-Nakonieczna ◽  
Anna Pytlak ◽  
Jarosław Grządziel ◽  
Adam Kubaczyński ◽  
Artur Banach ◽  
...  

Methanogenesis occurs in many natural environments and is used in biotechnology for biogas production. The efficiency of methane production depends on the microbiome structure that determines interspecies electron transfer. In this research, the microbial community retrieved from mining subsidence reservoir sediment was used to establish enrichment cultures on media containing different carbon sources (tryptone, yeast extract, acetate, CO2/H2). The microbiome composition and methane production rate of the cultures were screened as a function of the substrate and transition stage. The relationships between the microorganisms involved in methane formation were the major focus of this study. Methanogenic consortia were identified by next generation sequencing (NGS) and functional genes connected with organic matter transformation were predicted using the PICRUSt approach and annotated in the KEGG. The methane production rate (exceeding 12.8 mg CH4 L−1 d−1) was highest in the culture grown with tryptone, yeast extract, and CO2/H2. The analysis of communities that developed on various carbon sources casts new light on the ecophysiology of the recently described bacterial phylum Caldiserica and methanogenic Archaea representing the genera Methanomassiliicoccus and Methanothrix. Furthermore, it is hypothesized that representatives of Caldiserica may support hydrogenotrophic methanogenesis.


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