Production of bioethanol and xylitol from non-detoxified corn cob via a two-stage fermentation strategy

2020 ◽  
Vol 310 ◽  
pp. 123427 ◽  
Author(s):  
Cong Du ◽  
Yimin Li ◽  
Han Zong ◽  
Tangguo Yuan ◽  
Wenjie Yuan ◽  
...  
Keyword(s):  
Corn Cob ◽  
Two Stage ◽  
Fermentation Strategy

scholarly journals Enhanced acarbose production by Streptomyces M37 using a two-stage fermentation strategy

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0166985 ◽  
Author(s):  
Fei Ren ◽  
Long Chen ◽  
Shuangli Xiong ◽  
Qunyi Tong
Keyword(s):  
Two Stage ◽  
Fermentation Strategy

scholarly journals Sequential Production of ᴅ-xylonate and Ethanol from Non-Detoxified Corncob at Low-pH by Pichia kudriavzevii via a Two-Stage Fermentation Strategy

2021 ◽  
Vol 7 (12) ◽  
pp. 1038
Author(s):  
Hao Ji ◽  
Ke Xu ◽  
Xiameng Dong ◽  
Da Sun ◽  
Libo Jin
Keyword(s):  
Ph Control ◽  
Low Ph ◽  
Economic Viability ◽  
Excellent Performance ◽  
Two Stage ◽  
Pichia Kudriavzevii ◽  
Comprehensive Utilization ◽  
Ph Conditions ◽  
Fermentation Strategy ◽  
Simultaneous Saccharification

Improving the comprehensive utilization of sugars in lignocellulosic biomass is a major challenge for enhancing the economic viability of lignocellulose biorefinement. A robust yeast Pichia kudriavzevii N-X showed excellent performance in ethanol production under high temperature and low pH conditions and was engineered for ᴅ-xylonate production without xylitol generation. The recombinant strain P. kudriavzevii N-X/S1 was employed for sequential production of ᴅ-xylonate and ethanol from ᴅ-xylose, feeding on ᴅ-glucose without pH control in a two-stage strategy of aerobic and shifting micro-aerobic fermentation. Acid-pretreated corncob without detoxification and filtration was used for ᴅ-xylonate production, then simultaneous saccharification and ethanol fermentation was performed with cellulase added at pH 4.0 and at 40 °C. By this strategy, 33.5 g/L ᴅ-xylonate and 20.8 g/L ethanol were produced at yields of 1.10 g/g ᴅ-xylose and 84.3% of theoretical value, respectively. We propose a promising approach for the sequential production of ᴅ-xylonate and ethanol from non-detoxified corncob using a single microorganism.

scholarly journals Accumulation of γ‐aminobutyric acid by E nterococcus avium 9184 in scallop solution in a two‐stage fermentation strategy

2015 ◽  
Vol 9 (4) ◽  
pp. 478-485 ◽  
Author(s):  
Haoyue Yang ◽  
Ronge Xing ◽  
Linfeng Hu ◽  
Song Liu ◽  
Pengcheng Li
Keyword(s):  
Aminobutyric Acid ◽  
Two Stage ◽  
Fermentation Strategy

Exploring two-stage fermentation strategy of polyhydroxyalkanoate production using Aeromonas hydrophila

2013 ◽  
Vol 78 ◽  
pp. 80-84 ◽  
Author(s):  
Bor-Yann Chen ◽  
Jhao-Yin Hung ◽  
Tz-Jau Shiau ◽  
Yu-Hong Wei
Keyword(s):  
Aeromonas Hydrophila ◽  
Two Stage ◽  
Fermentation Strategy

scholarly journals Combined Deacetylation and PFI Refining Pretreatment of Corn Cob for the Improvement of a Two-Stage Enzymatic Hydrolysis

2014 ◽  
Vol 62 (20) ◽  
pp. 4661-4667 ◽  
Author(s):  
Yuedong Zhang ◽  
Xindong Mu ◽  
Haisong Wang ◽  
Bin Li ◽  
Hui Peng
Keyword(s):  
Enzymatic Hydrolysis ◽  
Corn Cob ◽  
Two Stage ◽  
Pfi Refining

scholarly journals Enhancement of Bacillus subtilis Growth and Sporulation by Two-Stage Solid-State Fermentation Strategy

Processes ◽  
2019 ◽  
Vol 7 (10) ◽  
pp. 644
Author(s):  
Zhao ◽  
Xi ◽  
Xu ◽  
Ma ◽  
Zhao
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Cell Growth ◽  
Solid State Fermentation ◽  
Active Sites ◽  
Cell Number ◽  
Two Stage ◽  
Fermentation Time ◽  
Second Stage ◽  
Fermentation Strategy

Two-stage solid-state fermentation strategy was exploited and systematically optimized to enhance Bacillus subtilis growth and sporulation for increasing effective cell number in B. subtilis microbial ecological agents. The first stage focused on improving cell growth followed by the second stage aiming to enhance both cell growth and sporulation. The optimal fermentation condition was that temperature changed from 37 °C to 47 °C at a fermentation time of 48 h and Mn2+ content in medium was 4.9 mg MnSO4/g dry medium. Solid medium properties were improved by the optimal two-stage fermentation. HPLC results demonstrated that glucose utilization was facilitated and low-field nuclear magnetic resonance (LF-NMR) results showed that more active sites in medium for microbial cells were generated during the optimal two-stage fermentation. Moreover, microbial growth and sporulation were enhanced simultaneously during the second stage of fermentation through delaying microbial decline phase and increasing sporulation rate. As a result, effective cell number of B. subtilis reached 1.79 × 1010/g dry medium after fermentation for 72 h, which was 29.7% and 8.48% higher than that of conventional fermentation for 72 h and 48 h, respectively. Therefore, the optimal two-stage fermentation could increase the effective cell number of B. subtilis microbial ecological agents efficiently.

Two-stage self-seeding method for preparation of single crystals of poly(phenylene sulfide)

1989 ◽  
Vol 47 ◽  
pp. 368-369
Author(s):  
Sengshiu Chung ◽  
Peggy Cebe
Keyword(s):  
Single Crystals ◽  
High Performance ◽  
Dilute Solution ◽  
Two Stage ◽  
Annealing Behavior ◽  
High Performance Polymers ◽  
Seeding Method ◽  
Phenylene Sulfide

We are studying the crystallization and annealing behavior of high performance polymers, like poly(p-pheny1ene sulfide) PPS, and poly-(etheretherketone), PEEK. Our purpose is to determine whether PPS, which is similar in many ways to PEEK, undergoes reorganization during annealing. In an effort to address the issue of reorganization, we are studying solution grown single crystals of PPS as model materials.Observation of solution grown PPS crystals has been reported. Even from dilute solution, embrionic spherulites and aggregates were formed. We observe that these morphologies result when solutions containing uncrystallized polymer are cooled. To obtain samples of uniform single crystals, we have used two-stage self seeding and solution replacement techniques.

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