Profuse color-evolution-based fluorescent test paper sensor for rapid and visual monitoring of endogenous Cu2+ in human urine

2018 ◽  
Vol 99 ◽  
pp. 332-337 ◽  
Author(s):  
Yueqing Cai ◽  
Junhui You ◽  
Zhengyi You ◽  
Fang Dong ◽  
Shuhu Du ◽  
...  
Keyword(s):  
Human Urine ◽  
Test Paper ◽  
Visual Monitoring ◽  
Color Evolution ◽  
Paper Sensor

scholarly journals An ink-jet printed dual-CD ratiometric fluorescent paper-based sensor for the visual detection of Cu2+

RSC Advances ◽  
2021 ◽  
Vol 11 (52) ◽  
pp. 33036-33047
Author(s):  
Ying Li ◽  
Fei Lu ◽  
Qing-zhi Li ◽  
Yi-hua Zhou ◽  
Jun Qian ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Carbon Dots ◽  
Visual Detection ◽  
Quantitative Detection ◽  
Test Paper ◽  
Photoluminescence Quantum Yield ◽  
Ink Jet ◽  
Paper Sensor

A fluorescent test paper sensor for qualitative and semi-quantitative detection of Cu2+ is designed based on high photoluminescence quantum yield (PLQY) carbon dots (CDs).

X-ray Spectrographic Microanalysis of Human Urine for Arsenic

1974 ◽  
Vol 28 (2) ◽  
pp. 165-170 ◽  
Author(s):  
James C. Mathies
Keyword(s):  
Standard Deviation ◽  
Human Body ◽  
Human Urine ◽  
Test Paper ◽  
X Ray ◽  
Paper Disk ◽  
Filter Paper Disk ◽  
Relative Standard ◽  
Digestion Mixture

A rapid, precise, and interference-free x-ray spectrographic procedure for the determination of arsenic in human body fluids and tissues has been devised. The organic material in the sample is oxidized, and the arsenic is released by a simplified rapid wet-washing technique using nitric-sulfuric-perchloric acids. The arsenic in the digestion mixture is converted to arsine and collected quantitatively on a silver nitrate-impregnated filter paper disk using a new and convenient sub-micro modification of the Gutzeit arsine generator. The arsenic in the test paper is rapidly and nondestructively quantitated to the nearest 0.1 µg in the x-ray spectrograph. The method is relatively free of the interferences usually associated with Gutzeit and colorimetric techniques for arsenic. The precision of the method is indicated by the fact that 5 µg of arsenic can be quantitated with a relative standard deviation of 2.5% or less. Normal levels of arsenic in human urine have been redetermined.

Visual monitoring of trace water in organic solvents based on ecofriendly b/r-CDs ratiometric fluorescence test paper

Talanta ◽  
2020 ◽  
Vol 216 ◽  
pp. 120958
Author(s):  
Xiqing Liu ◽  
Zhiping Zhou ◽  
Tao Wang ◽  
Pengyin Deng ◽  
Yongsheng Yan
Keyword(s):  
Organic Solvents ◽  
Test Paper ◽  
Ratiometric Fluorescence ◽  
Visual Monitoring ◽  
Fluorescence Test ◽  
Trace Water

A highly selective and dual responsive test paper sensor of Hg2+/Cr3+ for naked eye detection in neutral water

RSC Advances ◽  
2012 ◽  
Vol 2 (9) ◽  
pp. 3714 ◽  
Author(s):  
Priyadip Das ◽  
Amrita Ghosh ◽  
Hitesh Bhatt ◽  
Amitava Das
Keyword(s):  
Test Paper ◽  
Eye Detection ◽  
Naked Eye ◽  
Dual Responsive ◽  
Neutral Water ◽  
Paper Sensor ◽  
Naked Eye Detection

Separation of Plasminogen Activators from Human Uterine Tissue and a Comparison with Activators from Human Urine and Porcine Tissue

1979 ◽  
Vol 41 (04) ◽  
pp. 718-733 ◽  
Author(s):  
Preben Kok
Keyword(s):  
Plasminogen Activator ◽  
Human Urine ◽  
Gel Filtration ◽  
Ammonium Thiocyanate ◽  
Plasminogen Activators ◽  
Particle Sizes ◽  
Uterine Tissue ◽  
Porcine Tissue ◽  
Major Activity ◽  
Proliferative Phase

SummaryThree types of plasminogen activator could be distinguished in extracts from human uterine tissue. The activators differed in thermostability or in mode of inhibition by EACA.All the extracts contained stable as well as labile activators. The saline extracts were uniformly inhibited by increasing concentrations of EACA. Extracts made with 2 M ammonium thiocyanate were either uniformly inhibited by EACA or showed deflections indicating contamination with an activator, which was inhibited in a biphasic manner. It was possible to distinguish between: (1) An activator, abundantly present in the tissue, which was uniformly inhibited and stable. (2) Another uniformly inhibited activator, which was labile. (3) An activator, inhibited in a biphasic manner, similar to urokinase, which was present in varying amounts in uteri with the endometrium in the proliferative phase.Gel filtration of the uterine extracts showed two major activity peaks corresponding to particle sizes of 60,000 dalton and about 10,000 dalton.Antiserum to purified plasminogen activator, prepared from porcine ovaries, inhibited the activity of the human uterine extracts, but not the activities of human urokinase or urine. Urokinase antiserum in a concentration completely inhibiting human urine or urokinase, inhibited only 10% or less of the activities of human uterine extracts.

Soluble Thrombomodulin Purified from Human Urine Exhibits a Potent Anticoagulant Effect In Vitro and In Vivo

1995 ◽  
Vol 73 (05) ◽  
pp. 805-811 ◽  
Author(s):  
Yasuo Takahashi ◽  
Yoshitaka Hosaka ◽  
Hiromi Niina ◽  
Katsuaki Nagasawa ◽  
Masaaki Naotsuka ◽  
...  
Keyword(s):  
Human Urine ◽  
Specific Activity ◽  
Anticoagulant Activity ◽  
Rabbit Lung ◽  
Soluble Thrombomodulin ◽  
Molecular Weights ◽  
Dose Dependent Fashion ◽  
Dose Dependent

SummaryWe examined the anticoagulant activity of two major molecules of soluble thrombomodulin purified from human urine. The apparent molecular weights of these urinary thrombomodulins (UTMs) were 72,000 and 79,000, respectively. Both UTMs showed more potent cofactor activity for protein C activation [specific activity >5,000 thrombomodulin units (TMU)/mg] than human placental thrombomodulin (2,180 TMU/mg) and rabbit lung thrombomodulin (1,980 TMU/mg). The UTMs prolonged thrombin-induced fibrinogen clotting time (>1 TMU/ml), APTT (>5 TMU/ml), TT (>5 TMU/ml) and PT (>40 TMU/ml) in a dose-dependent fashion. These effects appeared in the concentration range of soluble thrombomodulins present in human plasma and urine. In the rat DIC model induced by thromboplastin, administration of UTMs by infusion (300-3,000 TMU/kg) restored the hematological abnormalities derived from DIC in a dose-dependent fashion. These results demonstrate that UTMs exhibit potent anticoagulant and antithrombotic activities, and could play a physiologically important role in microcirculation.

Rapid Isolation of High Molecular Weight Urokinase from Native Human Urine

1982 ◽  
Vol 47 (03) ◽  
pp. 197-202 ◽  
Author(s):  
Kurt Huber ◽  
Johannes Kirchheimer ◽  
Bernd R Binder
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Human Urine ◽  
Gel Filtration ◽  
Chain Structure ◽  
The Other ◽  
Single Chain ◽  
Apparent Homogeneity ◽  
Sequential Adsorption

SummaryUrokinase (UK) could be purified to apparent homogeneity starting from crude urine by sequential adsorption and elution of the enzyme to gelatine-Sepharose and agmatine-Sepharose followed by gel filtration on Sephadex G-150. The purified product exhibited characteristics of the high molecular weight urokinase (HMW-UK) but did contain two distinct entities, one of which exhibited a two chain structure as reported for the HMW-UK while the other one exhibited an apparent single chain structure. The purification described is rapid and simple and results in an enzyme with probably no major alterations. Yields are high enough to obtain purified enzymes for characterization of UK from individual donors.

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