Evaluation of a biosurfactant producing bacterial strain Pseudomonas sp. ISTPY2 for efficient pyrene degradation and landfill soil bioremediation through soil microcosm and proteomic studies

This article submits results of laboratory and field experiments on the effect of an auxin-producing bacterial strain Pseudomonas sp. DA1.2 in comparison with Pseudomonas koreensis IB-4 on wheat plants in conjunction with the “Chistalan” herbicide treatment. Our work shows the positive effect of bacterial treatments on plant growth, the relative water content in leaves and the role of bacteria in the redistribution of ABA and IAA in wheat shoots under conditions of herbicidal stress. Application of Pseudomonas sp. DA1.2 together with the herbicide in the field of the steppe zone led to an increase in yield by 20% relative to the control variant. This bacterial strain helps to overcome herbicidal stress and is a promising agent for improving the technology of using synthetic auxins herbicides.

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Optimizing the Management of Cadmium Bioremediation Capacity of Metal-Resistant Pseudomonas sp. Strain Al-Dhabi-126 Isolated from the Industrial City of Saudi Arabian Environment

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16234788 ◽

2019 ◽

Vol 16 (23) ◽

pp. 4788 ◽

Cited By ~ 4

Author(s):

Naif Abdullah Al-Dhabi ◽

Galal Ali Esmail ◽

Abdul-Kareem Mohammed Ghilan ◽

Mariadhas Valan Arasu

Keyword(s):

Bacterial Strain ◽

Cadmium Toxicity ◽

Industrial Effluents ◽

Pseudomonas Sp ◽

Bacterial Strains ◽

16S Rdna Gene ◽

Sequence Characterization ◽

Industrial City ◽

Toxic Pollutant ◽

Cd Removal

In this study, 23 bacterial strains were isolated from a Cadmium (Cd) contaminated soil in the industrial city, Riyadh of Saudi Arabia. Among these isolates six strains were found to withstand cadmium contamination and grow well. From the six isolates Pseudomonas sp. strain Al-Dhabi-122–127 were found to resist cadmium toxicity to a higher level. The isolates were subjected to biochemical and 16S rDNA gene sequence characterization to confirm their identification. The bacterial strain Al-Dhabi-124 showed 1.5 times higher Cd-degrading activity than Al-Dhabi-122 and Al-Dhabi-123, and Al-Dhabi-126 exhibited 3.5 times higher Cd-degrading activity, higher than the other strains. An atomic absorption spectrophotometer study showed that the strain Al-Dhabi-126 absorbed Cd, and that the bacterial strain Al-Dhabi-126 was found to tolerate cadmium level up to 2100 µg/mL. The bacterial strain Al-Dhabi-126 showed a maximum Cd removal efficacy at pH between 6.0 and 8.0. The efficacy decreased sharply after an increase in pH (9.0). An optimum temperature of 50 °C and pH 6.0 were found to be effective for the Cd removal process by the isolate. The study indicated that the bacterial strain Al-Dhabi-126 can be used effectively for the bioremediation of heavy metals like cadmium, a major toxic pollutant in industrial effluents.

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Functional Characterization and Genomic Analysis of the Chlorantraniliprole-Degrading Strain Pseudomonas Sp. GW13

Bioengineering ◽

10.3390/bioengineering6040106 ◽

2019 ◽

Vol 6 (4) ◽

pp. 106

Author(s):

Wa Gao ◽

Dongyang Li ◽

Hong You

Keyword(s):

Bacterial Strain ◽

Degradation Mechanism ◽

Functional Characterization ◽

Genomic Analysis ◽

Rrna Gene ◽

Pseudomonas Sp ◽

16S Rrna Gene Analysis ◽

Federal Institute ◽

Amide Hydrolysis ◽

Aquatic Science

Chlorantraniliprole (CAP) is a widely used insecticide in many areas due to its excellent insecticidal ability and mammalian safety, however, the removal of CAP has not been extensively studied. In this study, a bacterial strain GW13, which is capable of co-metabolizing CAP, was isolated from a vegetable field soil. The strain was identified as Pseudomonas sp. based on its physico-biochemical characteristics and 16S rRNA gene analysis. The bacterial strain GW13 could degrade CAP through co-metabolism, and glucose was the best additional carbon resource. In the presence of 1.0 g/L glucose, GW13 could co-metabolize over 80% of 200 mg/L CAP in 24 h. The degradation rate increased after 6 h and slowed again after 10 h. The GW13 genome analysis revealed many genes associated with metabolism, showing the degradation mechanism of GW13 from the genomic perspective. The EAWAG-BBD (Swiss Federal Institute of Aquatic Science and Technology Biocatalysis/Biodegradation Database) prediction results showed that the main pathway for CAP degradation is amide hydrolysis, which is consistent with many genes associated with amidase in the GW13 genome. This study may facilitate research on CAP biodegradation mechanisms in the environment.

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Drastic change in cell surface hydrophobicity of a new bacterial strain, Pseudomonas sp. TIS1-127, induced by growth temperature and its effects on the toluene-conversion rate

Journal of Bioscience and Bioengineering ◽

10.1016/j.jbiosc.2008.11.003 ◽

2009 ◽

Vol 107 (3) ◽

pp. 250-255 ◽

Cited By ~ 11

Author(s):

Katsutoshi Hori ◽

Naoto Hiramatsu ◽

Mari Nannbu ◽

Kei Kanie ◽

Mina Okochi ◽

...

Keyword(s):

Cell Surface ◽

Growth Temperature ◽

Bacterial Strain ◽

Conversion Rate ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Drastic Change ◽

Pseudomonas Sp ◽

Toluene Conversion

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Biosurfactant-Enhanced Remediation of Oil-Contaminated Environments

Adsorption Science & Technology ◽

10.1260/0263617001493369 ◽

2000 ◽

Vol 18 (2) ◽

pp. 171-176 ◽

Cited By ~ 13

Author(s):

A. Shulga ◽

E. Karpenko ◽

R. Vildanova-Martsishin ◽

A. Turovsky ◽

M. Soltys

Keyword(s):

Organic Contaminants ◽

Bacterial Strain ◽

High Surface ◽

Pseudomonas Sp ◽

Emulsifying Activity ◽

Glycolipid Biosurfactant ◽

Synthetic Materials ◽

Sea Sands ◽

Positive Results ◽

Enhanced Remediation

The development of new efficient cleaning technologies for the bioremediation of the environment and its cleaning from oil products has become a subject of great interest. Surfactants influence the bioremediation of oil and other organic contaminants through increasing their availability. Since surfactant products of a microbial nature are highly efficient in this respect, they have important advantages over synthetic materials. Being biodegradable, they are ecologically safe. A new washing bioremedy based on surfactant products synthesized microbially from the bacterial strain Pseudomonas sp. PS-17 has been obtained. It contains an unique biocomplex of a glycolipid biosurfactant and an alginate biopolymer and possesses a high surface and emulsifying activity. The washing and antisorptional capacity of this bioremedy has been tested on objects of various natures, viz. bird feathers, animal skins and furs. We have obtained positive results in the remediation of sea sands, birds and animals suffering from ecological catastrophy. It was shown that the bioremedy has mild properties and does not change the structure of the feathers, skins and furs studied. The bioremedy remains active over the temperature range 15–90°C.

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Treatment of Textile Dyes by Bio-chemical Process in Stirred Tank Sequencing Batch Bioreactor (STSBBR)

Journal of Chemical Engineering ◽

10.3329/jce.v27i2.17808 ◽

2014 ◽

Vol 27 (2) ◽

pp. 83-87

Author(s):

Pradip Saha ◽

MR Khan ◽

TK Deb ◽

SY Sony ◽

AC Baishnab

Keyword(s):

Waste Water ◽

Negative Correlation ◽

Bacterial Strain ◽

Chemical Engineering ◽

Stirred Tank ◽

Pseudomonas Sp ◽

Stirred Tank Bioreactor ◽

Batch Mode ◽

Batch Bioreactor ◽

Working Volume

A bacterial isolate Pseudomonas sp. was isolated from the solid waste slump collected from a local duping site of Sylhet district and was efficiently utilized for the removal of dye (Orange 3R) from simulated synthetic waste water in a lab scale Stirred Tank Sequencing Batch Bioreactor (STSBBR) in batch mode. A reactor with 2 L capacity (working volume 0.5 L) equipped with suitable control means and stirring mechanism was operated at room temperature and pH 6.6 ± 1 in fill-react-settling-draw mode with different initial dye concentrations (50, 100, 150, 200 & 300 ppm) where the hydraulic retention time was maintained for 12-72 hours depending on the adaptation of waste water by the bacterial strain. The efficiency of the reactor was analyzed with respect to three strands and found to be negative correlation with the concentration of the dye. Overall color, COD, and BOD in the Stirred tank bioreactor system (STSBBR) were removed by 49.67, 37.45 and 33.89%, respectively with 50 ppm dye concentration and HRT of 24 h. The efficiency of the reactor was found to be in negative correlation with the concentration of the dye. This STSBBR system was found very effective for efficient biological treatment of such dyeing industry waste water by the bacterial strain Pseudomonas sp. DOI: http://dx.doi.org/10.3329/jce.v27i2.17808 Journal of Chemical Engineering, IEB Vol. ChE. 27, No. 2, December 2012: 77-82

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