scholarly journals Optimizing the Management of Cadmium Bioremediation Capacity of Metal-Resistant Pseudomonas sp. Strain Al-Dhabi-126 Isolated from the Industrial City of Saudi Arabian Environment

2019 ◽  
Vol 16 (23) ◽  
pp. 4788 ◽  
Author(s):  
Naif Abdullah Al-Dhabi ◽  
Galal Ali Esmail ◽  
Abdul-Kareem Mohammed Ghilan ◽  
Mariadhas Valan Arasu
Keyword(s):  
Bacterial Strain ◽  
Cadmium Toxicity ◽  
Industrial Effluents ◽  
Pseudomonas Sp ◽  
Bacterial Strains ◽  
16S Rdna Gene ◽  
Sequence Characterization ◽  
Industrial City ◽  
Toxic Pollutant ◽  
Cd Removal

In this study, 23 bacterial strains were isolated from a Cadmium (Cd) contaminated soil in the industrial city, Riyadh of Saudi Arabia. Among these isolates six strains were found to withstand cadmium contamination and grow well. From the six isolates Pseudomonas sp. strain Al-Dhabi-122–127 were found to resist cadmium toxicity to a higher level. The isolates were subjected to biochemical and 16S rDNA gene sequence characterization to confirm their identification. The bacterial strain Al-Dhabi-124 showed 1.5 times higher Cd-degrading activity than Al-Dhabi-122 and Al-Dhabi-123, and Al-Dhabi-126 exhibited 3.5 times higher Cd-degrading activity, higher than the other strains. An atomic absorption spectrophotometer study showed that the strain Al-Dhabi-126 absorbed Cd, and that the bacterial strain Al-Dhabi-126 was found to tolerate cadmium level up to 2100 µg/mL. The bacterial strain Al-Dhabi-126 showed a maximum Cd removal efficacy at pH between 6.0 and 8.0. The efficacy decreased sharply after an increase in pH (9.0). An optimum temperature of 50 °C and pH 6.0 were found to be effective for the Cd removal process by the isolate. The study indicated that the bacterial strain Al-Dhabi-126 can be used effectively for the bioremediation of heavy metals like cadmium, a major toxic pollutant in industrial effluents.

scholarly journals Effect of TiO2 on Selected Pathogenic and Opportunistic Intestinal Bacteria

2021 ◽  
Author(s):  
Ewa Baranowska-Wójcik ◽  
Dominik Szwajgier ◽  
Klaudia Gustaw
Keyword(s):  
Titanium Dioxide ◽  
Bacterial Strain ◽  
Intestinal Bacteria ◽  
Food Additive ◽  
Bacterial Strains ◽  
Gastrointestinal Microbiota ◽  
Food Grade ◽  
The Eu

AbstractFood-grade titanium dioxide (TiO2) containing a nanoparticle fraction (TiO2 NPs-nanoparticles) is widely used as a food additive (E171 in the EU). In recent years, questions concerning its effect on the gastrointestinal microbiota have been raised. In the present study, we examined interactions between bacteria and TiO2. The study involved six pathogenic/opportunistic bacterial strains and four different-sized TiO2 types: three types of food-grade E171 compounds and TiO2 NPs (21 nm). Each bacterial strain was exposed to four concentrations of TiO2 (60, 150, 300, and 600 mg/L TiO2). The differences in the growth of the analyzed strains, caused by the type and concentration of TiO2, were observed. The growth of a majority of the strains was shown to be inhibited after exposure to 300 and 600 mg/L of the food-grade E171 and TiO2 NPs.

Genetics of symbiosis and nitrogen fixation in legumes

1969 ◽  
Vol 172 (1029) ◽  
pp. 417-437 ◽  
Keyword(s):  
Nitrogen Fixation ◽  
Bacterial Strain ◽  
Host Plants ◽  
Strain Differences ◽  
Homogeneous Material ◽  
Developmental Sequence ◽  
Bacterial Strains ◽  
Nodule Bacteria ◽  
Microbial Genetics ◽  
Nodular Tissue

Soon after the isolation of nodule bacteria in 1888, differences were recognized in the ability of bacterial strains to form nodules on particular host plants and in the nitrogen-fixing ability of the nodules so formed. These and other symbiotic heterogeneities were attributed, sometimes correctly, to bacterial strain differences, not then thought to be open to formal genetic analysis. The realization that the host plant was an essential component of this variability came only gradually, stimulated by observations of host varietal differences and by the demand for reliable and homogeneous material for experimental work. Only within the last two decades has host variability been studied by plant breeding, and bacterial strain differences by some of the methods of microbial genetics. This review, except for a brief reference to earlier work of some historic interest, will consider only genetic problems open to investigation by these methods. The developmental sequence in all legume nodules is broadly similar. The initial infection phases are followed by the induction of the nodule, the invasion of part of the nodular tissue and culminate in bacteroid formation and nitrogen fixation; the genetics of symbiosis will be considered in this context.

scholarly journals Agroecological aspects of application of Pseudomonas sp. DA1.2 in overcoming herbicidal stress in wheat

2020 ◽  
Vol 23 ◽  
pp. 03009
Author(s):  
Maxim. D. Timergalin ◽  
Arina V. Feoktistova ◽  
Timur V. Rameev ◽  
Gaisar G. Khudaygulov ◽  
Sergei N. Starikov ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Field Experiments ◽  
Steppe Zone ◽  
Pseudomonas Sp ◽  
Synthetic Auxins ◽  
Promising Agent ◽  
Relative Water ◽  
Stress Application ◽  
Positive Effect

This article submits results of laboratory and field experiments on the effect of an auxin-producing bacterial strain Pseudomonas sp. DA1.2 in comparison with Pseudomonas koreensis IB-4 on wheat plants in conjunction with the “Chistalan” herbicide treatment. Our work shows the positive effect of bacterial treatments on plant growth, the relative water content in leaves and the role of bacteria in the redistribution of ABA and IAA in wheat shoots under conditions of herbicidal stress. Application of Pseudomonas sp. DA1.2 together with the herbicide in the field of the steppe zone led to an increase in yield by 20% relative to the control variant. This bacterial strain helps to overcome herbicidal stress and is a promising agent for improving the technology of using synthetic auxins herbicides.

scholarly journals Studies on decolorization of textile dye by using Pseudomonas and bacillus sp from the contaminated effluent soil samples of Kovilpatti, Thoothukudi district of Tamil Nadu

2019 ◽  
Vol 11 (1) ◽  
pp. 134-137 ◽  
Author(s):  
Kannan D ◽  
Renuga Devi ◽  
A. G. Murugesan ◽  
S. Rajan
Keyword(s):  
Contaminated Soil ◽  
Tamil Nadu ◽  
Soil Samples ◽  
Toxic Chemicals ◽  
Textile Dye ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Textile Industries ◽  
Source Of Pollution

Textile industries releasing large amount of effluent which contains textile dyes and toxic chemicals and it is one of the major source of pollution also contaminating water bodies. To remove that, bacteria have been of great attention because of their ability to treat effluent. The present study was undertaken to exploit the ability of Pseudomonassp and Bacillus sp from dye contaminated soil samples for bioremediation for dye effluent. Among the bacterial strains used in the study. Pseudomonas sp emerged out to be most potent decolorizer in comparison to Bacillus sp with the degree of decolorization of 90.0 %. Thus, it was concluded that the Pseudomonas sp had highest color removing capacity from contaminated effluent soil samples. 

Dicyano-bis(pyridin-2,6-dicarbothioato)-ferrat (II)/ferrat (III), ein weiteres eisenhaltiges Redoxsystem aus der Kulturlösung eines Pseudomonas-Stammes [1] / Dicyano-bis(pyridin-2,6-dicarbothioato)-ferrate (II)/ferrate (III), a Further Fe Containing Redox System from the Culture Medium of Pseudomonas sp.

1985 ◽  
Vol 40 (3-4) ◽  
pp. 201-207 ◽  
Author(s):  
U. Hildebrand ◽  
K. Taraz ◽  
H. Budzikiewicz ◽  
H. Korth ◽  
G. Pulverer
Keyword(s):  
Pseudomonas Putida ◽  
Culture Medium ◽  
Redox System ◽  
Pseudomonas Sp ◽  
Redox Potentials ◽  
Bacterial Strains ◽  
Normal Potential

From the culture medium of Pseudomonas sp. a further Fe containing complex, viz. dicyano- bis(pyridin-2,6-dicarbothioato)-ferrate (III) (2) has been isolated which participates in a ferrate (II)/ferrate (III) redox system (normal potential of-0.013 V) in the range of the redox potentials of cytochromes. Pyridine-2,6-di(monothiocarboxylic acid) which originally has been considered to be characteristic for Pseudomonas putida has been found recently as a metabolite of other bacterial strains (two of which have been characterized in this paper) as well.

scholarly journals Opposite Enantioselectivities of Two Phenotypically and Genotypically Similar Strains of Pseudomonas frederiksbergensis in Bacterial Whole-Cell Sulfoxidation

2005 ◽  
Vol 71 (4) ◽  
pp. 2199-2202 ◽  
Author(s):  
Waldemar Adam ◽  
Frank Heckel ◽  
Chantu R. Saha-Möller ◽  
Marcus Taupp ◽  
Jean-Marie Meyer ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Soil Samples ◽  
Bacterial Strains ◽  
Whole Cell ◽  
Organic Sulfides

ABSTRACT Soil samples were screened to select microorganisms with the capability to oxidize organic sulfides into the corresponding sulfoxides with differential enantioselectivities. Several bacterial strains that preferentially produced the S-configured sulfoxide enantiomer were isolated. Surprisingly, one bacterial strain, genotypically and phenotypically characterized as Pseudomonas frederiksbergensis, selectively gave the R enantiomer. The finding that two apparently identical organisms displayed opposite enantioselectivities is novel for non-genetically modified organisms.

scholarly journals Bacterial Consortiums Able to Use Metal-Cyanide Complexes as a Nitrogen Source

Proceedings ◽  
2018 ◽  
Vol 2 (20) ◽  
pp. 1284
Author(s):  
María Isabel Igeño ◽  
Daniel Macías ◽  
María Isabel Guijo ◽  
Rubén Sánchez-Clemente ◽  
Ana G. Población ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Industrial Effluents ◽  
Free Form ◽  
Bacterial Strains ◽  
Pseudomonas Pseudoalcaligenes ◽  
Metal Cyanide ◽  
Biodegradation Process ◽  
Cyanide Complexes ◽  
Cyano Compounds

Most cyanide-containing industrial effluents also contain other cyano-derivatives and high amounts of metals and metal-cyanide compounds. For this reason, the biotreatment of these wastes requires the use of microorganisms capable to degrade all these different cyano-compounds and to tolerate metals. Pseudomonas pseudoalcaligenes CECT 5344 is a cyanotrophic bacterium capable of metabolize cyanide in its free form, but it is not very efficient at degrading metal-cyanide complexes. Therefore, for the optimization of the cyanide biodegradation process it is essential to find and characterize new bacterial strains, capable of assimilating metal cyanide-complexes, to complement the capacities of P. pseudoalcaligenes CECT 5344.

scholarly journals Bacillus subtilis Strain Engineered for Treatment of Soil-Transmitted Helminth Diseases

2013 ◽  
Vol 79 (18) ◽  
pp. 5527-5532 ◽  
Author(s):  
Yan Hu ◽  
Melanie M. Miller ◽  
Alan I. Derman ◽  
Brian L. Ellis ◽  
Rose Gomes Monnerat ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacterial Strain ◽  
Subtilis Strain ◽  
Human Consumption ◽  
Bacterial Strains ◽  
Content Type ◽  
Live Bacteria ◽  
Therapeutic Properties ◽  
Adverse Environmental Conditions ◽  
Hookworm Infections

ABSTRACTSoil-transmitted helminths (hookworms, whipworms, and large roundworms) are agents of intestinal roundworm diseases of poverty that infect upwards of 2 billion people worldwide. A great challenge in treating these diseases is the development of anthelmintic therapeutics that are inexpensive, can be produced in great quantity, and are capable of delivery under varied and adverse environmental conditions. A potential solution to this challenge is the use of live bacteria that are acceptable for human consumption, e.g.,Bacillus subtilis, and that can be engineered with therapeutic properties. In this study, we expressed theBacillus thuringiensisanthelmintic protein Cry5B in a bacterial strain that has been used as a model for live bacterial therapy,Bacillus subtilisPY79. PY79 transformed with a Cry5B expression plasmid (PY79-Cry5B) is able to express Cry5B from the endogenousB. thuringiensis cry5Bpromoter. During sporulation of PY79-Cry5B, Cry5B is packaged as a crystal. Furthermore, Cry5B produced in PY79 is bioactive, with a 50% lethal concentration (LC50) of 4.3 μg/ml against the roundwormCaenorhabditis elegans. PY79-Cry5B was a significantly effective therapeutic in experimentalAncylostoma ceylanicumhookworm infections of hamsters. A single 10-mg/kg (0.071 μmol/kg of body weight) dose of Cry5B administered as a Cry5B-PY79 spore crystal lysate achieved a 93% reduction in hookworm burdens, which is superior on a molar level to reductions seen with clinically used anthelmintics. Given that a bacterial strain such as this one can be produced cheaply in massive quantities, our results demonstrate that the engineering and delivery of live bacterial strains have great potential to treat a significant contributor to poverty worldwide, namely, hookworm disease and other soil-transmitted helminthiasis.

688. Phage-organism relationships among strains ofStreptococcus cremoris: the selection of strains as cheese starters

1957 ◽  
Vol 24 (3) ◽  
pp. 381-386 ◽  
Author(s):  
H. R. Whitehead ◽  
Elizabeth J. Bush
Keyword(s):  
Host Range ◽  
Bacterial Strain ◽  
Bacterial Strains ◽  
Clear Cut ◽  
Phage Multiplication ◽  
Selection Of ◽  
Family Groups

The relationships between nineteen strains ofStr. cremorisand nineteen phage races were investigated. It was found that in addition to clear-cut lytic reactions there were other actions between some phage races and bacterial strains whereby in some cases a phage could show adaptation to a strain and become changed in host range, and in other cases could inhibit growth of a strain without any phage multiplication.There is some evidence to indicate that bacterial strains and phage races fall into family groups within which most of the phages act in one way or another on most of the strains. The suggestion is made that the strains and races in a group may have originated from a parent bacterial strain and parent phage race.The selection of strains ofStr. cremorisfor use as cheese starters is discussed in the light of the findings reported in the present paper. The diffculty of isolating a large number of strains unrelated in every respect is indicated.

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