scholarly journals Structure-based design and biological evaluation of inhibitors of the pseudomonas aeruginosa heme oxygenase (pa-HemO)

2018 ◽  
Vol 28 (6) ◽  
pp. 1024-1029 ◽  
Author(s):  
Dongdong Liang ◽  
Elizabeth Robinson ◽  
Kellie Hom ◽  
Wenbo Yu ◽  
Nam Nguyen ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Heme Oxygenase ◽  
Biological Evaluation

scholarly journals De novo synthesis, structural assignment and biological evaluation of pseudopaline, a metallophore produced by Pseudomonas aeruginosa

2019 ◽  
Vol 10 (27) ◽  
pp. 6635-6641 ◽  
Author(s):  
Jian Zhang ◽  
Tianhu Zhao ◽  
Rongwen Yang ◽  
Ittipon Siridechakorn ◽  
Sanshan Wang ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Total Synthesis ◽  
Natural Product ◽  
Absolute Configuration ◽  
De Novo ◽  
Biological Evaluation ◽  
De Novo Synthesis ◽  
Structural Assignment ◽  
The Absolute

The first total synthesis and isolation of pseudopaline was reported, which allows determination and confirmation of the absolute configuration of the natural product.

scholarly journals Design, synthesis and biological evaluation of 1,2,3-triazole based 2-aminobenzimidazoles as novel inhibitors of LasR dependent quorum sensing in Pseudomonas aeruginosa

RSC Advances ◽  
2019 ◽  
Vol 9 (50) ◽  
pp. 29273-29292 ◽  
Author(s):  
Singireddi Srinivasarao ◽  
Adinarayana Nandikolla ◽  
Shashidhar Nizalapur ◽  
Tsz Tin Yu ◽  
Sravani Pulya ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Cell Line ◽  
Biological Evaluation ◽  
Design Synthesis ◽  
Hek 293 ◽  
Line P ◽  
Synthesis And Biological Evaluation ◽  
Hek 293 Cell Line

Out of 40 benzimdazoles, 12 exhibited potent QSI activity against P. aeruginosa6p, most active QSI is docked to LasR and is less toxic against HEK 293 cell line.

scholarly journals Homologues of Neisserial Heme Oxygenase in Gram-Negative Bacteria: Degradation of Heme by the Product of thepigA Gene of Pseudomonas aeruginosa

2001 ◽  
Vol 183 (21) ◽  
pp. 6394-6403 ◽  
Author(s):  
Melanie Ratliff ◽  
Wenming Zhu ◽  
Rahul Deshmukh ◽  
Angela Wilks ◽  
Igor Stojiljkovic
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Heme Oxygenase ◽  
High Performance ◽  
Visible Spectrum ◽  
Inducible Promoter ◽  
Major Product ◽  
Gene Encoding ◽  
Heme Oxygenases ◽  
Chromatography Analysis ◽  
Biliverdin Ix

ABSTRACT The oxidative cleavage of heme to release iron is a mechanism by which some bacterial pathogens can utilize heme as an iron source. ThepigA gene of Pseudomonas aeruginosa is shown to encode a heme oxygenase protein, which was identified in the genome sequence by its significant homology (37%) with HemO ofNeisseria meningitidis. When the gene encoding the neisserial heme oxygenase, hemO, was replaced withpigA, we demonstrated that pigA could functionally replace hemO and allow for heme utilization by neisseriae. Furthermore, when pigA was disrupted by cassette mutagenesis in P. aeruginosa, heme utilization was defective in iron-poor media supplemented with heme. This defect could be restored both by the addition of exogenous FeSO4, indicating that the mutant did not have a defect in iron metabolism, and by in trans complementation with pigA from a plasmid with an inducible promoter. The PigA protein was purified by ion-exchange chromotography. The UV-visible spectrum of PigA reconstituted with heme showed characteristics previously reported for other bacterial and mammalian heme oxygenases. The heme-PigA complex could be converted to ferric biliverdin in the presence of ascorbate, demonstrating the need for an exogenous reductant. Acidification and high-performance liquid chromatography analysis of the ascorbate reduction products identified a major product of biliverdin IX-β. This differs from the previously characterized heme oxygenases in which biliverdin IX-α is the typical product. We conclude that PigA is a heme oxygenase and may represent a class of these enzymes with novel regiospecificity.

The effects of novel heme oxygenase inhibitors on the growth of Pseudomonas aeruginosa

2019 ◽  
Vol 129 ◽  
pp. 64-67
Author(s):  
Jingming Zhao ◽  
Dongdong Liang ◽  
Elizabeth Robinson ◽  
Fengtian Xue
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Heme Oxygenase

scholarly journals Iminoguanidines as Allosteric Inhibitors of the Iron-Regulated Heme Oxygenase (HemO) of Pseudomonas aeruginosa

2016 ◽  
Vol 59 (14) ◽  
pp. 6929-6942 ◽  
Author(s):  
Geoffrey A. Heinzl ◽  
Weiliang Huang ◽  
Wenbo Yu ◽  
Bennett J. Giardina ◽  
Yue Zhou ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Heme Oxygenase ◽  
Allosteric Inhibitors

Synthesis, Characterization, and Biological Evaluation of Novel Pseudomonas aeruginosa RTAC 11 Synthesized Ag Nanoparticles

2020 ◽  
Vol 18 (9) ◽  
pp. 694-699
Author(s):  
Lavina Temkar ◽  
Ritika Tripathi ◽  
Abhishekh Tiwari ◽  
Navjeet Kaur ◽  
Arivarasan Vishnu Kirthi ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Spectroscopic Analysis ◽  
Biological Evaluation ◽  
Toxic Chemicals ◽  
Biological Synthesis ◽  
Good Antibacterial Activity ◽  
Vis Spectroscopy ◽  
Nano Size ◽  
Harmful Effects

Biological synthesis of silver nanoparticles (AgNPs) has been an area of research to avoid the harmful effects of the toxic chemicals used in its synthesis on our health and environment. In the present study, a novel strain of Pseudomonas aeruginosa-RTAC 11 isolated from soil was used for the synthesis of AgNPs. Characterization of the synthesized nanoparticles like UV-vis spectroscopy confirmed the synthesis of AgNPs through a signature peak at 420 nm. Fourier Transform Infrared (FTIR) spectroscopic analysis revealed the capping of AgNPs by proteins through signature functional groups. The nano-size of the particles were confirmed using scanning electron microscopy (SEM). The AgNPs showed good antibacterial activity against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 6538, thus indicating an excellent antibacterial agent.

Synthesis and biological evaluation of novel pyrimidines derived from 6-aryl-5-cyano-2-thiouracil

2016 ◽  
Vol 71 (5-6) ◽  
pp. 133-140 ◽  
Author(s):  
Asma Mohamed Mahran ◽  
Nasser Abdelhamid Hassan ◽  
Dalia Ahmed A. Osman ◽  
Sherif Shaban Ragab ◽  
Allam Abdelhamid Hassan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antifungal Agents ◽  
Biological Evaluation ◽  
Microbial Activities ◽  
Gram Positive ◽  
Fungal Strains ◽  
Gram Positive Bacteria ◽  
Synthesis And Biological Evaluation

Abstract Starting from 6-aryl-5-cyano-2-thiouracil derivative 1a–f, a series of novel thiazolo[3,2-a]pyrimidines 4a–f were synthesized. The mechanism and the regioselectivity of the studied reactions are discussed. In addition, a series of tetrahydro-4-H-pyrimido[2,1-b][1,3]thiazines 7a–e and 2-((ethoxymethyl)thio)-4-aryl-1,6-dihydropyrimidines 9b,c,e were synthesized. The anti-microbial activities of some of the prepared compounds were screened, and the results revealed that compounds 3c and 4c were more active than the standard (Ampicillin) against gram positive bacteria (Pseudomonas aeruginosa). Moreover, compounds 4b,e and 3f were found to be good antifungal agents against the studied fungal strains.

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