Quantification of bone tissue regeneration employing β-tricalcium phosphate by three-dimensional non-invasive synchrotron micro-tomography — A comparative examination with histomorphometry

Bone ◽  
2009 ◽  
Vol 44 (4) ◽  
pp. 619-628 ◽  
Author(s):  
Michael Stiller ◽  
Alexander Rack ◽  
Simon Zabler ◽  
Jürgen Goebbels ◽  
Olaf Dalügge ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Tricalcium Phosphate ◽  
Three Dimensional ◽  
Bone Tissue Regeneration ◽  
Non Invasive ◽  
Micro Tomography ◽  
Comparative Examination

scholarly journals Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 61 ◽  
Author(s):  
Yannan Liu ◽  
Juan Gu ◽  
Daidi Fan
Keyword(s):  
Mechanical Properties ◽  
Enzymatic Hydrolysis ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Repair ◽  
Three Dimensional ◽  
High Strength ◽  
Biological Properties ◽  
Bone Tissue Regeneration

A novel, three-dimensional, porous, human-like collagen (HLC)/nano-hydroxyapatite (n-HA) scaffold cross-linked by 1,2,7,8-diepoxyoctane (DEO) was successfully fabricated, which showed excellent mechanical and superior biological properties for bone tissue regeneration in this study. The physicochemical characterizations of different n-HA/HLC/DEO (nHD) scaffolds were investigated by determining the morphology, compression stress, elastic modulus, Young’s modulus and enzymatic hydrolysis behavior in vitro. The results demonstrated that nHD-2 and nHD-3 scaffolds showed superior mechanical properties and resistance to enzymatic hydrolysis compared to nHD-1 scaffolds. The cell viability, live cell staining and cell adhesion analysis results demonstrated that nHD-2 scaffolds exhibited low cytotoxicity and excellent cytocompatibility compared with nHD-1 and nHD-3 scaffolds. Furthermore, subcutaneous injections of nHD-2 scaffolds in rabbits produced superior anti-biodegradation effects and histocompatibility compared with injections of nHD-1 and nHD-3 scaffolds after 1, 2 and 4 weeks. In addition, the repair of bone defects in rabbits demonstrated that nHD-2 scaffolds presented an improved ability for guided bone regeneration and reconstruction compared to commercially available bone scaffold composite hydroxyapatite/collagen (HC). Collectively, the results show that nHD-2 scaffolds show promise for application in bone tissue engineering due to their excellent mechanical properties, anti-biodegradation, anti-biodegradation, biocompatibility and bone repair effects.

Hybrid Tricalcium Phosphate/Hydrogel Constructs Functionalized with an Antitumor Drug for Bone Tissue Regeneration

2020 ◽  
Vol 11 (5) ◽  
pp. 1144-1152
Author(s):  
P. A. Karalkin ◽  
N. S. Sergeyeva ◽  
I. K. Sviridova ◽  
V. A. Kirsanova ◽  
S. A. Akhmedova ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Tricalcium Phosphate ◽  
Antitumor Drug ◽  
Bone Tissue Regeneration

Three-Dimensional Scaffolds of Carbonized Polyacrylonitrile for Bone Tissue Regeneration

2014 ◽  
Vol 126 (35) ◽  
pp. 9367-9371 ◽  
Author(s):  
Seungmi Ryu ◽  
Choonghyeon Lee ◽  
Jooyeon Park ◽  
Jun Seop Lee ◽  
Seokyung Kang ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Three Dimensional ◽  
Bone Tissue Regeneration

scholarly journals Bioink Formulations for Bone Tissue Regeneration

2021 ◽  
Vol 9 ◽  
Author(s):  
Na Li ◽  
Rui Guo ◽  
Zhenyu Jason Zhang
Keyword(s):  
Extracellular Matrix ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Three Dimensional ◽  
Bone Tissue Regeneration ◽  
3D Bioprinting ◽  
Native Bone ◽  
Technical Requirements ◽  
Tissue Scaffolding ◽  
Functional Transformations

Unlike the conventional techniques used to construct a tissue scaffolding, three-dimensional (3D) bioprinting technology enables fabrication of a porous structure with complex and diverse geometries, which facilitate evenly distributed cells and orderly release of signal factors. To date, a range of cell-laden materials, such as natural or synthetic polymers, have been deployed by the 3D bioprinting technique to construct the scaffolding systems and regenerate substitutes for the natural extracellular matrix (ECM). Four-dimensional (4D) bioprinting technology has attracted much attention lately because it aims to accommodate the dynamic structural and functional transformations of scaffolds. However, there remain challenges to meet the technical requirements in terms of suitable processability of the bioink formulations, desired mechanical properties of the hydrogel implants, and cell-guided functionality of the biomaterials. Recent bioprinting techniques are reviewed in this article, discussing strategies for hydrogel-based bioinks to mimic native bone tissue-like extracellular matrix environment, including properties of bioink formulations required for bioprinting, structure requirements, and preparation of tough hydrogel scaffolds. Stimulus mechanisms that are commonly used to trigger the dynamic structural and functional transformations of the scaffold are analyzed. At the end, we highlighted the current challenges and possible future avenues of smart hydrogel-based bioink/scaffolds for bone tissue regeneration.

Apatite Foam Fabrication Based on Hydrothermal Reaction of α-Tricalcium Phosphate Foam

2007 ◽  
Vol 361-363 ◽  
pp. 319-322 ◽  
Author(s):  
Ishikawa Kunio ◽  
Satoshi Karashima ◽  
Akari Takeuchi ◽  
Shigeki Matsuya
Keyword(s):  
Pore Size ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Tricalcium Phosphate ◽  
Hydrothermal Reaction ◽  
Bone Substitutes ◽  
Tissue Response ◽  
Bone Tissue Regeneration ◽  
Setting Reaction ◽  
Interconnected Pores

Apatite foam (AP foam) is an ideal material for bone substitutes and scaffolds in bone tissue regeneration. This is because its highly porous interconnected pores provide the space for cell growth and tissue penetration, and that its composition induces excellent tissue response and good osteoconductivity. In the present study, the feasibility of apatite foam fabrication was evaluated based on so-called dissolution-reprecipitation reaction of α-tricalcium phosphate (α-TCP) foam granules. When α-TCP foam granules were placed in water at 37°C for 24h, no reaction was observed. However, α-TCP foam set to form AP foam when treated hydrothermally at 200°C. The network of fully interconnected pores was retained, and porosity was as high as 82%. Pore size ranged from 50 to 300 0m with average pore size at 160 0m. Compressive strength was 207 kPa. Although no setting reaction was observed at 37°C, setting reaction caused by hydrothermal treatment of α-TCP foam granules at 200°C allows AP foam of any shape to be fabricated. Therefore, this method was suggested to be useful for the fabrication of bone substitutes and the scaffold in bone tissue regeneration.

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