In vitro skin decontamination of the organophosphorus pesticide Paraoxon with nanometric cerium oxide CeO 2

2017 ◽  
Vol 267 ◽  
pp. 57-66 ◽  
Author(s):  
Alicia Salerno ◽  
Thierry Devers ◽  
Marie-Alexandrine Bolzinger ◽  
Jocelyne Pelletier ◽  
Denis Josse ◽  
...  
Keyword(s):  
Cerium Oxide ◽  
Organophosphorus Pesticide ◽  
Skin Decontamination

Cerium oxide nanomaterial with dual antioxidative scavenging potential: Synthesis and characterization

2021 ◽  
pp. 088532822110134
Author(s):  
Sushant Singh ◽  
Udit Kumar ◽  
David Gittess ◽  
Tamil S Sakthivel ◽  
Balaashwin Babu ◽  
...  
Keyword(s):  
Cerium Oxide ◽  
Biological Systems ◽  
Mixed Valence ◽  
Stable Solution ◽  
Ability To Act ◽  
Valence States ◽  
Transmission Electron ◽  
Efficient Reduction ◽  
Rich Media

Many studies have linked reactive oxygen species (ROS) to various diseases. Biomedical research has therefore sought a way to control and regulate ROS produced in biological systems. In recent years, cerium oxide nanoparticles (nanoceria, CNPs) have been pursued due to their ability to act as regenerative ROS scavengers. In particular, they are shown to have either superoxide dismutase (SOD) or catalase mimetic (CAT) potential depending on the ratio of Ce3+/Ce4+ valence states. Moreover, it has been demonstrated that SOD mimetic activity can be diminished by the presence of phosphate, which can be a problem given that many biological systems operate in a phosphate-rich environment. Herein, we report a CNP formulation with both SOD and catalase mimetic activity that is preserved in a phosphate-rich media. Characterization demonstrated a highly dispersed, stable solution of uniform-sized, spherical-elliptical shaped CNP of 12 ± 2 nm, as determined through dynamic light scattering, zeta potential, and transmission electron microscopy. Mixed valence states of Ce ions were observed via UV/Visible spectroscopy and XPS (Ce3+/Ce4+ > 1) (Ce3+∼ 62%). X-ray diffraction and XPS confirmed the presence of oxygen-deficient cerium oxide (CeO2-x) particles. Finally, the CNP demonstrated very good biocompatibility and efficient reduction of hydrogen peroxide under in-vitro conditions.

scholarly journals Cerium oxide nanoparticles improve liver regeneration after acetaminophen-induced liver injury and partial hepatectomy in rats

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Bernat Córdoba-Jover ◽  
Altamira Arce-Cerezo ◽  
Jordi Ribera ◽  
Montse Pauta ◽  
Denise Oró ◽  
...  
Keyword(s):  
Liver Injury ◽  
Liver Regeneration ◽  
Cerium Oxide ◽  
Partial Hepatectomy ◽  
Experimental Models ◽  
Cerium Oxide Nanoparticles ◽  
Hepatic Regeneration ◽  
Oxide Nanoparticles ◽  
Acetyl Cysteine

Abstract Background and aims Cerium oxide nanoparticles are effective scavengers of reactive oxygen species and have been proposed as a treatment for oxidative stress-related diseases. Consequently, we aimed to investigate the effect of these nanoparticles on hepatic regeneration after liver injury by partial hepatectomy and acetaminophen overdose. Methods All the in vitro experiments were performed in HepG2 cells. For the acetaminophen and partial hepatectomy experimental models, male Wistar rats were divided into three groups: (1) nanoparticles group, which received 0.1 mg/kg cerium nanoparticles i.v. twice a week for 2 weeks before 1 g/kg acetaminophen treatment, (2) N-acetyl-cysteine group, which received 300 mg/kg of N-acetyl-cysteine i.p. 1 h after APAP treatment and (3) partial hepatectomy group, which received the same nanoparticles treatment before partial hepatectomy. Each group was matched with vehicle-controlled rats. Results In the partial hepatectomy model, rats treated with cerium oxide nanoparticles showed a significant increase in liver regeneration, compared with control rats. In the acetaminophen experimental model, nanoparticles and N-acetyl-cysteine treatments decreased early liver damage in hepatic tissue. However, only the effect of cerium oxide nanoparticles was associated with a significant increment in hepatocellular proliferation. This treatment also reduced stress markers and increased cell cycle progression in hepatocytes and the activation of the transcription factor NF-κB in vitro and in vivo. Conclusions Our results demonstrate that the nanomaterial cerium oxide, besides their known antioxidant capacities, can enhance hepatocellular proliferation in experimental models of liver regeneration and drug-induced hepatotoxicity.

scholarly journals In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates

Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1565 ◽  
Author(s):  
Turin-Moleavin ◽  
Fifere ◽  
Lungoci ◽  
Rosca ◽  
Coroaba ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Iron Oxide ◽  
Cerium Oxide ◽  
Chemical Activation ◽  
Radical Scavenging ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles

Background. Cerium oxide nanoparticles present the mimetic activity of superoxide dismutase, being able to inactivate the excess of reactive oxygen species (ROS) correlated with a large number of pathologies, such as stents restenosis and the occurrence of genetic mutations that can cause cancer. This study presents the synthesis and biological characterisation of nanoconjugates based on nanoparticles of iron oxide interconnected with cerium oxide conjugates. Methods. The synthesis of magnetite-nanoceria nanoconjugates has been done in several stages, where the key to the process is the coating of nanoparticles with polyethyleneimine and its chemical activation-reticulation with glutaraldehyde. The nanoconjugates are characterised by several techniques, and the antioxidant activity was evaluated in vitro and in vivo. Results. Iron oxide nanoparticles interconnected with cerium oxide nanoparticles were obtained, having an average diameter of 8 nm. Nanoconjugates prove to possess superparamagnetic properties and the saturation magnetisation varies with the addition of diamagnetic components in the system, remaining within the limits of biomedical applications. In vitro free-radical scavenging properties of nanoceria are improved after the coating of nanoparticles with polyethylenimine and conjugation with magnetite nanoparticles. In vivo studies reveal increased antioxidant activity in all organs and fluids collected from mice, which demonstrates the ability of the nanoconjugates to reduce oxidative stress. Conclusion. Nanoconjugates possess magnetic properties, being able to scavenge free radicals, reducing the oxidative stress. The combination of the two properties mentioned above makes them excellent candidates for theranostic applications.

scholarly journals In vitro inhibition of human red blood cell acetylcholinesterase (AChE) by temephos-oxidized products

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Francisco Alberto Verdín-Betancourt ◽  
Mario Figueroa ◽  
Ma. de Lourdes López-González ◽  
Elizabeth Gómez ◽  
Yael Yvette Bernal-Hernández ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Organophosphorus Pesticide ◽  
Oxidation Products ◽  
Toxicological Studies ◽  
Biological And Environmental Samples ◽  
Metabolic Products ◽  
Chlorinated Drinking Water ◽  
Oxidized Products

Abstract Temephos (Tem) is an organophosphorus pesticide widely used to kill and prevent the growth of the main vectors for the transmission of dengue, zika, and chikungunya viruses. In chlorinated water, Tem is oxidized to its dioxon-sulfoxide (Tem-dox-SO), dioxon-sulfone (Tem-dox-SO2), and sulfoxide (Tem-SO) derivatives; however, these compounds are not commercially available to be used as standards and in toxicological studies. In the present study, we synthesized and characterized the Tem-oxidation products and the compound 4,4′-sulfinyldiphenol. These compounds were obtained by a simple reaction between Tem or 4,4′-thiodiphenol with sodium hypochlorite or potassium periodate, and were characterized by IR, NMR, and UPLC-HRESIMS. The in vitro evaluation of inhibitory potency of Tem-oxidized products on human red blood cell acetylcholinesterase (RBC AChE) showed that Tem-dox-SO2 was the most potent inhibitor of human RBC AChE, and its effect was more pronounced than that observed for ethyl-paraoxon, a potent typical inhibitor of AChE. An HPLC-DAD method for the analysis of metabolic products of Tem was developed, which may be useful for monitoring in biological and environmental samples. The ability of Tem-oxidized metabolites to inhibit human RBC AChE suggests that the addition of Tem to chlorinated drinking water could result in an increase in the risk of RBC AChE inhibition after exposure.

scholarly journals Planarian cholinesterase: in vitro characterization of an evolutionarily ancient enzyme to study organophosphorus pesticide toxicity and reactivation

2016 ◽  
Vol 91 (8) ◽  
pp. 2837-2847 ◽  
Author(s):  
Danielle Hagstrom ◽  
Hideto Hirokawa ◽  
Limin Zhang ◽  
Zoran Radic ◽  
Palmer Taylor ◽  
...  
Keyword(s):  
Organophosphorus Pesticide ◽  
Pesticide Toxicity ◽  
In Vitro Characterization

scholarly journals Impact of cerium oxide nanoparticles shape on their in vitro cellular toxicity

2017 ◽  
Vol 38 ◽  
pp. 136-141 ◽  
Author(s):  
Valérie Forest ◽  
Lara Leclerc ◽  
Jean-François Hochepied ◽  
Adeline Trouvé ◽  
Gwendoline Sarry ◽  
...  
Keyword(s):  
Cerium Oxide ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Cellular Toxicity

scholarly journals In vitro effects of organophosphorus pesticide malathion on the reactivity of rat aorta

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Agnes Afrodite Sumarelli Albuquerqu Fagundes ◽  
Marco Túlio Menezes Carvalho ◽  
Luciana Garros Ferreira ◽  
Ana Paula Cassiano Silveira ◽  
Tales Rubens Nadai ◽  
...  
Keyword(s):  
Organophosphorus Pesticide ◽  
Rat Aorta ◽  
In Vitro Effects

Proteome Mediated Synthesis of Biocompatible Green Fluorescence Cerium Oxide Quantum Dots with Enhanced Antioxidant Activity

2020 ◽  
Vol 12 (6) ◽  
pp. 831-839
Author(s):  
Amita A. Bhagit ◽  
Sveeta V. Mhatre ◽  
R. P. Yadav
Keyword(s):  
Quantum Dots ◽  
Antioxidant Activity ◽  
Band Gap ◽  
Cerium Oxide ◽  
Bulk Material ◽  
Degree Of Crystallinity ◽  
Light Quantum ◽  
Vis Spectroscopy

Biocompatibility of quantum dots make good candidature for in vivo and in vitro diagnostic applications. In this study biocompatible cerium oxide quantum dots (CeO2 QDs) were synthesized from proteome of Justicia adhatoda leaf using simple aqueous protocol. Synthesized cerium oxide nanoparticle possessed green colored fluorescence under UV light. Quantum yield of CeO2 QDs was found to be 31.13% and were stable for 3 month at 4 °C. The band gap was found to be 3.26 eV which was higher than the band gap of bulk material i.e., 3.19 eV. The concentration of synthesized CeO2 QDs was determined as 165.96 ppm. These quantum dots were also characterized using an UV-Vis spectroscopy, Fourier Transform Infra-red Spectroscopy (FT-IR) and Transmission Electron Microscopy (TEM) respectively. It displayed distinct absorbance peak at 380 nm under UV-Visible spectrum. TEM images showed monodispersity of 2–5 nm spherical shaped CeO2 QDs and higher degree of crystallinity was observed by the pattern of selected area electron diffraction. In FTIR, the stretching observed at 621.65 cm–1 is assigned to Ce-O which confirmed the CeO2QDs formation. In view of biocompability, synthesized CeO2QDs were also characterised in reference to cell toxicity. Generated CeO2QDs has not showed any toxicity to J774A.1, Raw 264.7 and 3T3 cell lines in vitro cell viability assay. Significant enhancement in antioxidant activity of synthesized cerium oxide nanoparticles was observed from bulk material. This process offers plenty of advantages such as simple protocol, mild environment operation, potential for large scale commercial production of biocompatible CeO2QD. These QDs might find potential applications in both in vitro and in vivo diagnostics.

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