Hsp70s transcription-translation relationship depends on the heat shock temperature in zebrafish

2020 ◽  
Vol 240 ◽  
pp. 110629 ◽  
Author(s):  
Giovanna Mottola ◽  
Mikko Nikinmaa ◽  
Katja Anttila
Keyword(s):  
Heat Shock ◽  
Shock Temperature

Return temperature after heat shock affects the production of tetraploids in the yellowtail tetra Astyanax altiparanae

Zygote ◽  
2020 ◽  
pp. 1-5
Author(s):  
Leandro Freitas Martins ◽  
Cleonice Cristina Hilbig ◽  
George Shigueki Yasui ◽  
Paulo Sérgio Monzani ◽  
José Augusto Senhorini ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Heat Shock ◽  
Embryonic Development ◽  
Control Groups ◽  
Temperature Shock ◽  
Future Studies ◽  
Shock Control ◽  
Shock Temperature ◽  
Post Shock ◽  
Basic Biology

Summary The aim of this study was to evaluate different post-shock temperatures for tetraploid induction in the yellowtail tetra Astyanax altiparanae. Newly fertilized eggs were divided into four groups, three were submitted to heat shock (40°C for 2 min) at 24 min post-fertilization (mpf) and another group remained without shock (control). Groups submitted to temperature shock were further separated at the following temperatures: 22°C, 26°C and 28°C. Survival among embryonic development was counted and at hatching the ploidy was analyzed by flow cytometry. The results showed that the post-shock temperature affects the parameters analyzed and, therefore, must be considered for optimization of the production of tetraploid in A. altiparanae. Those data are innovative and could be used in future studies of basic biology in this species.

Slow heating of barley aleurone layers to heat-shock temperature preserves heat-shock-sensitive cellular properties

2002 ◽  
Vol 89 (3) ◽  
pp. 401-409 ◽  
Author(s):  
Mark K. Johnston ◽  
Paul A. S. Benson ◽  
Tracy M. Rodgers ◽  
Mark R. Brodl
Keyword(s):  
Heat Shock ◽  
Slow Heating ◽  
Shock Temperature ◽  
Barley Aleurone

Changes in photosynthetic parameters and antioxidant activities following heat-shock treatment in tomato plants

2006 ◽  
Vol 33 (2) ◽  
pp. 177 ◽  
Author(s):  
Daymi Camejo ◽  
Ana Jiménez ◽  
Juan José Alarcón ◽  
Walfredo Torres ◽  
Juana María Gómez ◽  
...  
Keyword(s):  
Heat Shock ◽  
Antioxidant Activities ◽  
Dark Respiration ◽  
Photosynthetic Apparatus ◽  
Photosynthetic Parameters ◽  
Tomato Plants ◽  
Shock Temperature ◽  
Oxidative Processes ◽  
Gas Exchange Characteristics ◽  
Chlorophyll Photooxidation

Seedlings of two tomato genotypes, Lycopersicon esculentum Mill. var. Amalia and the wild thermotolerant type Nagcarlang, were grown under a photoperiod of 16 h light at 25°C and 8 h dark at 20°C. At the fourth true leaf stage, a group of plants were exposed to a heat-shock temperature of 45°C for 3 h, and measurements of chlorophyll fluorescence, gas-exchange characteristics, dark respiration and oxidative and antioxidative parameters were made after releasing the stress. The heat shock induced severe alterations in the photosynthesis of Amalia that seem to mitigate the damaging impact of high temperatures by lowering the leaf temperature and maintaining stomatal conductance and more efficient maintenance of antioxidant capacity, including ascorbate and glutathione levels. These effects were not evident in Nagcarlang. In Amalia plants, a larger increase in dark respiration also occurred in response to heat shock and the rates of the oxidative processes were higher than in Nagcarlang. This suggests that heat injury in Amalia may involve chlorophyll photooxidation mediated by activated oxygen species (AOS) and more severe alterations in the photosynthetic apparatus. All these changes could be related to the more dramatic effect of heat shock seen in Amalia than in Nagcarlang plants.

scholarly journals Sensitivity of Seedling Radicles to Chilling and Heat-shock-induced Chilling Tolerance

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 848C-848
Author(s):  
Abdur Rab ◽  
Mikal E. Saltveit
Keyword(s):  
Heat Shock ◽  
Mung Bean ◽  
Lateral Roots ◽  
Chilling Injury ◽  
Chilling Tolerance ◽  
Shock Temperature ◽  
Chilling Sensitivity ◽  
Hibiscus Esculentus ◽  
Time Required ◽  
Shock Proteins

Chilling sensitivity increased as the radicle of germinating corn (Zea mays L. `Jubilee' hybrid), cucumber (Cucumis sativus L. `Poinsett 76'), mung bean (Phaseolus aureus Roxb. `Berkin'), and tomato (Lycopersicon esculentum Mill. `Rio Grande') seeds increased in length from 1 to 7 mm. In contrast, radicles of germinating okra (Hibiscus esculentus L. `Clemson' spineless) seeds exhibited similar levels of chilling sensitivity at all radicle lengths. The degree of chilling sensitivity varied among the species in relation to time required to elicit a significant response and the magnitude of the elicited response. Based on subsequent radicle elongation, okra and cucumber were the most sensitive species to chilling at 2.5C for 96 h; tomato and corn were relatively less sensitive, and mung bean was the least sensitive. This pattern of sensitivities changed when other criteria were used to evaluate chilling sensitivity. The development of lateral roots decreased with prolonged chilling in all species, except for corn in which the apical tip remained viable even after 192 h of chilling. Heat shock (0 to 10 min at 45C) induced chilling tolerance in all species, except okra. In okra, neither increasing the heat shock temperature nor decreasing the severity of chilling (i.e., temperature and duration of exposure) resulted in a significant reduction in chilling injury. The differential induction of heat shock proteins in okra and the other species is discussed.

Tissue-specific variation in Hsp70 expression and thermal damage in Drosophila melanogaster larvae.

1997 ◽  
Vol 200 (14) ◽  
pp. 2007-2015 ◽  
Author(s):  
R A Krebs ◽  
M E Feder
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Trypan Blue ◽  
Fat Body ◽  
Malpighian Tubules ◽  
Blue Staining ◽  
Hsp70 Expression ◽  
Shock Temperature ◽  
Specific Variation ◽  
Imaginal Disks

All tissues of larval Drosophila melanogaster express Hsp70, the major heat-shock protein of this species, after both mild (36 degrees C) and severe (38.5 degrees C) heat shock. We used Hsp70-specific immunofluorescence to compare the rate and intensity of Hsp70 expression in various tissues after these two heat-shock treatments, and to compare this with related differences in the intensity of Trypan Blue staining shown by the tissues. Trypan Blue is a marker of tissue damage. Hsp70 was rarely detectable before heat shock. Brain, salivary glands, imaginal disks and hindgut expressed Hsp70 within the first hour of heat shock, whereas gut tissues, fat body and Malpighian tubules did not express Hsp70 until 4-21 h after heat shock. Differences in Hsp70 expression between tissues were more pronounced at the higher heat-shock temperature. Tissues that expressed Hsp70 slowly stained most intensely with Trypan Blue. Gut stained especially intensely, which suggests that its sensitivity to heat shock may limit larval thermotolerance. These patterns further suggest that some cells respond primarily to damage caused by heat shock rather than to elevated temperature per se and/or that Hsp70 expression is itself damaged by heat and requires time for recovery in some tissues.

Hsp85 conformational change within the heat shock temperature range

1992 ◽  
Vol 184 (1) ◽  
pp. 394-399 ◽  
Author(s):  
Karl W. Lanks ◽  
Erwin London ◽  
Dennis Long-Yu Dong
Keyword(s):  
Heat Shock ◽  
Conformational Change ◽  
Temperature Range ◽  
Shock Temperature

On the chaperonin activity of GroEL at heat-shock temperature

2005 ◽  
Vol 37 (7) ◽  
pp. 1375-1385 ◽  
Author(s):  
Girish C. Melkani ◽  
Gustavo Zardeneta ◽  
Jose A. Mendoza
Keyword(s):  
Heat Shock ◽  
Shock Temperature

Desiccation-induced dormancy in papaya (Carica papayaL.) seeds is alleviated by heat shock

2000 ◽  
Vol 10 (2) ◽  
pp. 135-145 ◽  
Author(s):  
Christopher B. Wood ◽  
Hugh W. Pritchard ◽  
Dilip Amritphale
Keyword(s):  
Heat Shock ◽  
Moisture Content ◽  
Seed Dormancy ◽  
Carica Papaya ◽  
Shock Treatment ◽  
Weight Basis ◽  
Heat Shock Treatment ◽  
Fresh Weight ◽  
Fresh Weight Basis ◽  
Shock Temperature

AbstractThe effects of desiccation and temperature on the germination capabilityof Carica papayaL. were investigated for seeds extracted from three commercial fruit batches. More than 50% of freshly isolated, cleaned (sarcotesta removed) but undried seeds germinated at 26°C. However, desiccation to approx. 20%seed RH reduced germination at this temperature to < 10%. A substantial increase in germination at alternating temperatures (33/19°C) indicated that desiccation induced seed dormancy rather than viability loss. Dormancy could be removed in a large proportion of the population by the application of a single heat shock to rehydrated seeds for 4 h at 36°C, with subsequent return to 26°C for germination. Longer (days) and shorter (minutes) periods of heat shock were less effective for releasing dormancy. Heat shock was generally applied 5 or 14 d after rehydration had started, but the treatment was equally effective after imbibition for only 1 d. Light was always applied during both imbibition and the post-heat shock treatment, but was not essential during the actual heat shock treatment. Rehydration and post-heat shock temperature treatments in the range of 16°C to 36°C revealed the same optima of 26°C. Dormancy was re-imposed in heat-shocked seeds when they were subsequently dried to seed relative humidities of 25 to 75%(5 to 11% moisture content [fresh weight basis]), but this state could be removed by a further heat shock. The longer heat-shocked seeds were held on agar-water at 26°C prior to re-drying, the greater the level of desiccation intolerance.

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