Return temperature after heat shock affects the production of tetraploids in the yellowtail tetra Astyanax altiparanae

Zygote ◽  
2020 ◽  
pp. 1-5
Author(s):  
Leandro Freitas Martins ◽  
Cleonice Cristina Hilbig ◽  
George Shigueki Yasui ◽  
Paulo Sérgio Monzani ◽  
José Augusto Senhorini ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Heat Shock ◽  
Embryonic Development ◽  
Control Groups ◽  
Temperature Shock ◽  
Future Studies ◽  
Shock Control ◽  
Shock Temperature ◽  
Post Shock ◽  
Basic Biology

Summary The aim of this study was to evaluate different post-shock temperatures for tetraploid induction in the yellowtail tetra Astyanax altiparanae. Newly fertilized eggs were divided into four groups, three were submitted to heat shock (40°C for 2 min) at 24 min post-fertilization (mpf) and another group remained without shock (control). Groups submitted to temperature shock were further separated at the following temperatures: 22°C, 26°C and 28°C. Survival among embryonic development was counted and at hatching the ploidy was analyzed by flow cytometry. The results showed that the post-shock temperature affects the parameters analyzed and, therefore, must be considered for optimization of the production of tetraploid in A. altiparanae. Those data are innovative and could be used in future studies of basic biology in this species.

Heat-induced triploids in Brycon amazonicus: a strategic fish species for aquaculture and conservation

Zygote ◽  
2021 ◽  
pp. 1-5
Author(s):  
Nivaldo Ferreira do Nascimento ◽  
Rafaela Manchin Bertolini ◽  
Lucia Soares Lopez ◽  
Laura Satiko Okada Nakaghi ◽  
Paulo Sérgio Monzani ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Heat Shock ◽  
Early Development ◽  
Fish Species ◽  
Survival Rates ◽  
Shock Treatment ◽  
Hatching Rate ◽  
Heat Shock Treatment ◽  
Control Survival ◽  
Ploidy Status

Summary Triploidization plays an important role in aquaculture and surrogate technologies. In this study, we induced triploidy in the matrinxã fish (Brycon amazonicus) using a heat-shock technique. Embryos at 2 min post fertilization (mpf) were heat shocked at 38°C, 40°C, or 42°C for 2 min. Untreated, intact embryos were used as a control. Survival rates during early development were monitored and ploidy status was confirmed using flow cytometry and nuclear diameter analysis of erythrocytes. The hatching rate reduced with heat-shock treatment, and heat-shock treatments at 42°C resulted in no hatching events. Optimal results were obtained at 40°C with 95% of larvae exhibiting triploidy. Therefore, we report that heat-shock treatments of embryos (2 mpf) at 40°C for 2 min is an effective way to induce triploid individuals in B. amazonicus.

scholarly journals Impaired Temperature Stress Response of a Streptococcus thermophilus deoD Mutant

2003 ◽  
Vol 69 (2) ◽  
pp. 1287-1289 ◽  
Author(s):  
Mario Varcamonti ◽  
Maria R. Graziano ◽  
Romilde Pezzopane ◽  
Gino Naclerio ◽  
Slavica Arsenijevic ◽  
...  
Keyword(s):  
Growth Rate ◽  
Stress Response ◽  
Heat Shock ◽  
Temperature Stress ◽  
Streptococcus Thermophilus ◽  
Wild Type ◽  
Temperature Shock ◽  
Dual Response ◽  
Survival Capacity

ABSTRACT An insertional deoD mutant of Streptococcus thermophilus strain SFi39 had a reduced growth rate at 20°C and an enhanced survival capacity to heat shock compared to the wild type, indicating that the deoD product is involved in temperature shock adaptation. We report evidence that ppGpp is implicated in this dual response.

Διαχρονικές μεταβολές βιοενέργειας, μεταβολισμού και ανοσολογικής απόκρισης στην οξεία φάση της σοβαρής σήψης

2018 ◽  
Author(s):  
Άννα-Μαρία Σπανάκη-Μπαρμπουνάκη
Keyword(s):  
Body Mass Index ◽  
Flow Cytometry ◽  
Severe Sepsis ◽  
Heat Shock ◽  
Energy Expenditure ◽  
Systemic Inflammatory Response Syndrome ◽  
Enzyme Linked Immunosorbent Assay ◽  
Z Scores ◽  
Luminescent Assay ◽  
Shock Proteins

Εισαγωγή: Το κυτταρικό στρες από σοβαρή σήψη (severe sepsis, SS) ή σύνδρομο συστηματικής φλεγμονώδους απάντησης (Systemic inflammatory response syndrome, SIRS) εκδηλώνεται με οξείες φλεγμονώδεις, ορμονικές, ανοσολογικές και μεταβολικές διαταραχές. Η συσχέτισή τους με πιθανή δυσλειτουργία μιτοχονδρίων δεν έχει επαρκώς μελετηθεί. Σκοπός: Σκοπός της μελέτης ήταν η εκτίμηση των διαχρονικών μεταβολών φλεγμονώδους-ορμονικής αντίδρασης, ενδογενούς-ανοσίας, βιοενέργειας και μεταβολισμού σε ασθενείς, ενήλικες και παιδιά, με σοβαρή σήψη (SS) και η σύγκριση με αντίστοιχες ομάδες ασθενών με SIRS και υγιών (H), ενηλίκων και παιδιών.Υλικά/Μέθοδοι: Μελετήθηκαν 68 παιδιά (SS/18, SIRS/23, H/27) και 79 ενήλικες (SS/23, SIRS/23, H/33) διαχρονικά, την 1, 3η και 5η ημέρα νοσηλείας. Υπολογίστηκαν ο δείκτης μάζας σώματος (Body mass index (BMI) z-scores) και τα scores βαρύτητας νόσου (PeLOD, APACHE, TISS, SOFA). Μετρήθηκαν η καρδιακή συσταλτικότητα (EF, SF), η τροπονίνη (Tn), το γαλακτικό οξύ, η κατανάλωση ενέργειας (Energy expenditure, EE) με Gas Module E-COVX, το ATP στα λευκά αιμοσφαίρια με δοκιμασία λουσιφεράσης (luciferase luminescent assay), τα επίπεδα γλουταμίνης και NO2/NO3 με υγρή χρωματογραφία υψηλής πίεσης (HPLC), τα προϊόντα υπεροξείδωσης λιπιδίων (TBARS) με χρωματομετρική δοκιμασία, η ρεζιστίνη, η αντιπονεκτίνη ορού και οι εξωκυττάριες Heat Shock Proteins (HSP) με την ποσοτική ανοσοενζυμική μέθοδο ELISA (sandwich enzyme-linked immunosorbent assay), και οι ενδοκυττάριες HSP72, HSP90α με κυτταρομετρία ροής (flow cytometry). Αποτελέσματα: Διαχρονικά τόσο σε ενήλικες (ICU) όσο και σε παιδιά (PICU) οι τιμές ρεζιστίνης, αντιπονεκτίνης, εξωκυττάριας HPS72 και 90α παρουσιάζαν σταθερό πρότυπο διέγερσης σε όλη την οξεία φάση των 5 ημέρων. Στη χρονική αυτή περίοδο, οι παράμετροι μεταβολισμού VO2, VCO2, EE παρουσίασαν σταθερό υπομεταβολικό προφίλ, ίδιο σε ενήλικες και παιδιά. Η αυξημένη έκφραση των NO3, NO2, TBARS και αντιπονεκτίνης στη σήψη παρουσίασαν μια πιο ασταθή εικόνα όσον αφορά τη διαχρονική τους έκφραση ανά ηλικιακή ομάδα.Η βιοενέργεια των μιτοχονδρίων ήταν διαχρονικά μειωμένη σε ενήλικες κα παιδιά που δεν επιβίωσαν σε σχέση με εκείνους που επιβίωσαν, και συνοδεύονταν από σημαντικά μειωμένο μεταβολισμό και υπομεταβολικά πρότυπα την 3η και 5η ημέρα (p<0.05). Οι ασθενείς που επιβίωσαν παρουσίαζαν σημαντική διαφοροποίηση των αρχικών τιμών BVR, γαλακτικού, ΕΕ, VO2, VCO2 και μεταβολικού προφιλ σε σύγκριση με ασθενείς που πέθαναν, οι οποίοι έδειξαν αδυναμία ανάκαμψης του υπομεταβολισμού ή της αντιοξειδωτικής κατάστασης την 5η ημέρα. Συμπέρασμα: Η SS χαρακτηρίζεται διαχρονικά, από ισχυρότερη ενδοκυττάρια καταστολή μεταβολισμού, μείωση κατανάλωσης ενέργειας, μείωση των ATP, HPS72, HSP90α, αλβουμίνης, γλουταμίνης και εξωκυττάρια αύξηση φλεγμονωδών ορμονών, ρεζιστίνης και αντιπονεκτίνης και πρωτεϊνών έμφυτης ανοσίας (HSP72). Μια πρώιμη κατάσταση υπομεταβολισμού, με καταστολή βιοενέργειας και ενδογενούς ανοσίας, η οποία και παραμένει διαχρονικά μαζί με συνεχιζόμενη κατάσταση φλεγμονής, με διαχρονικά αυξημένες μεταβολικές ορμόνες και πρωτείνες eHSP72/HSP90α, φαίνεται να ξεχωρίζει τη σήψη από το SIRS, και συνδέεται με αυξημένο κίνδυνο θανάτου.Λέξεις κλειδιά: σήψη, SIRS, βιοενέργεια, HSP, μιτοχόνδρια, μεταβολισμός, θερμιδομετρία, αμινοξέα, οξείδιο του αζώτου, ATP, ρεζιστίνη, αντιπονεκτίνη, τραύμα

scholarly journals How the Pilates Method Can Influence Sports Performance in Different Modalities

2020 ◽  
Vol 25 (264) ◽  
pp. 139-152
Author(s):  
Manoela Abreu ◽  
Franciele Carvalho Santos ◽  
Ana Laura Nogueira ◽  
Matheus Lima Zampieri ◽  
Dernival Bertoncello
Keyword(s):  
Systematic Review ◽  
Methodological Quality ◽  
Web Of Science ◽  
Sports Performance ◽  
Review Of The Literature ◽  
Control Groups ◽  
Future Studies ◽  
Pilates Method

The aim of this study was to perform a systematic review of the literature in order to investigate the effects of the Pilates Method on athletes of different sports. Methods: Researches were carried out in databases (SciELO, LILACS, PubMed, Web of Science and SCOPUS) and to evaluate the methodological quality of the studies, the PEDro scale was used. Results: Of the 87 studies found, only four were included. Meta-analyzes to assess flexibility using the Wells Bank's Sit and Reach test and a fleximeter indicated improvement after Pilates application, although there were no statistically significant differences compared to the control groups (Wells Bank's Sit and Reach test: 2 , 83 95% CI: -0.73 to 6.38, I² = 99%; Fleximeter: -0.78, 95% CI: -1.84 to 0.27, I² = 0%). Conclusion: There is evidence of benefits after Pilates intervention. Future studies with standardized protocols, according to the chosen sport, are necessary to determine how the Pilates Method can improve athletes' performance.

Promoter of the heat shock testis-specific Hsp70.2/Hst70 gene is active in nervous system during embryonic development of mice

2006 ◽  
Vol 211 (6) ◽  
pp. 631-638 ◽  
Author(s):  
Weronika Rupik ◽  
Agata Stawierej ◽  
Iwona Stolarczyk ◽  
Wieslawa Widłak
Keyword(s):  
Nervous System ◽  
Heat Shock ◽  
Embryonic Development

scholarly journals Potential caveats of putative microglia-specific markers for assessment of age-related cerebrovascular neuroinflammation

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Pedram Honarpisheh ◽  
Juneyoung Lee ◽  
Anik Banerjee ◽  
Maria P. Blasco-Conesa ◽  
Parisa Honarpisheh ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Ex Vivo ◽  
Myeloid Cells ◽  
Amyloid Angiopathy ◽  
Future Studies ◽  
Age Related ◽  
In Vivo Animal Models ◽  
Intermediate Expression ◽  
The Brain

Abstract Background The ability to distinguish resident microglia from infiltrating myeloid cells by flow cytometry-based surface phenotyping is an important technique for examining age-related neuroinflammation. The most commonly used surface markers for the identification of microglia include CD45 (low-intermediate expression), CD11b, Tmem119, and P2RY12. Methods In this study, we examined changes in expression levels of these putative microglia markers in in vivo animal models of stroke, cerebral amyloid angiopathy (CAA), and aging as well as in an ex vivo LPS-induced inflammation model. Results We demonstrate that Tmem119 and P2RY12 expression is evident within both CD45int and CD45high myeloid populations in models of stroke, CAA, and aging. Interestingly, LPS stimulation of FACS-sorted adult microglia suggested that these brain-resident myeloid cells can upregulate CD45 and downregulate Tmem119 and P2RY12, making them indistinguishable from peripherally derived myeloid populations. Importantly, our findings show that these changes in the molecular signatures of microglia can occur without a contribution from the other brain-resident or peripherally sourced immune cells. Conclusion We recommend future studies approach microglia identification by flow cytometry with caution, particularly in the absence of the use of a combination of markers validated for the specific neuroinflammation model of interest. The subpopulation of resident microglia residing within the “infiltrating myeloid” population, albeit small, may be functionally important in maintaining immune vigilance in the brain thus should not be overlooked in neuroimmunological studies.

scholarly journals Estimate of Plasma Temperatures Across a CME-Driven Shock from a Comparison Between EUV and Radio Data

Solar Physics ◽  
2020 ◽  
Vol 295 (9) ◽  
Author(s):  
Federica Frassati ◽  
Salvatore Mancuso ◽  
Alessandro Bemporad
Keyword(s):  
Shock Wave ◽  
Compression Ratio ◽  
Emission Measure ◽  
Type Ii ◽  
Solar Dynamics Observatory ◽  
Radio Data ◽  
Shock Temperature ◽  
Post Shock ◽  
Dem Analysis ◽  
Solar Dynamics

Abstract In this work, we analyze the evolution of an EUV wave front associated with a solar eruption that occurred on 30 October 2014, with the aim of investigating, through differential emission measure (DEM) analysis, the physical properties of the plasma compressed and heated by the accompanying shock wave. The EUV wave was observed by the Atmospheric Imaging Assembly (AIA) onboard the Solar Dynamics Observatory (SDO) and was accompanied by the detection of a metric Type II burst observed by ground-based radio spectrographs. The EUV signature of the shock wave was also detected in two of the AIA channels centered at 193 Å and 211 Å as an EUV intensity enhancement propagating ahead of the associated CME. The density compression ratio $X$ X of the shock as inferred from the analysis of the EUV data is $X \approx 1.23$ X ≈ 1.23 , in agreement with independent estimates obtained from the analysis of the Type II band-splitting of the radio data and inferred by adopting the upstream–downstream interpretation. By applying the Rankine–Hugoniot jump conditions under the hypothesis of a perpendicular shock, we also estimate the temperature ratio as $T_{\mathrm{D}}/T_{\mathrm{U}} \approx 1.55$ T D / T U ≈ 1.55 and the post-shock temperature as $T_{\mathrm{D}}\approx 2.75$ T D ≈ 2.75 MK. The modest compression ratio and temperature jump derived from the EUV analysis at the shock passage are typical of weak coronal shocks.

Effects of a Curriculum-Integrated Dance Program on Children’s Physical Activity

2020 ◽  
Vol 17 (11) ◽  
pp. 1179-1183
Author(s):  
Geeta Sharma ◽  
Tom Stewart ◽  
Scott Duncan
Keyword(s):  
Physical Activity ◽  
New Zealand ◽  
Control Group ◽  
Control Groups ◽  
Intervention Effects ◽  
Future Studies ◽  
School Time ◽  
And Control ◽  
The Impact ◽  
Children’S Physical Activity

Background: Curriculum-integrated dance programs are a promising but relatively under-researched strategy for increasing children’s physical activity (PA). The aim of this study was to determine the impact of a curriculum-integrated dance program on children’s PA. Methods: A total of 134 primary children aged 7–9 years from 4 New Zealand schools were assigned to either a dance group (n = 78) or a control group (n = 56). The dance group participated in a 6-week curriculum-integrated dance program during school time. Although the dance program focused on curricular learning, fitness and coordination were embedded in the dance sessions. Intensity of PA varied according to the focus of each dance session. PA was measured at baseline and postintervention using a waist-mounted ActiGraph GT3X+ accelerometer for 8 consecutive days. Results: There were no significant intervention effects on PA levels between the dance and control groups postintervention. Conclusion: Dance-embedded learning did not increase overall levels of PA in this study. Future studies may consider assessing longer term effects of a dance-based intervention, or programs that place more focus on PA promotion.

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