Single-step integrated technology for enhanced CO2 biofixation and efficient lipid extraction in microalgal system including a water-immiscible solvent

2021 ◽  
pp. 134374
Author(s):  
Liju Bai ◽  
Shijian Lu ◽  
Shun Qiu ◽  
Jintong Li ◽  
Siming Chen
Keyword(s):  
Lipid Extraction ◽  
Single Step ◽  
Integrated Technology ◽  
Co2 Biofixation

scholarly journals Comparison of different lipid extraction methods for different tissue types of Arabidopsis thaliana

2020 ◽  
Author(s):  
Cheka Kehelpannala ◽  
Thusitha Wasantha Thilaka Rupasinghe ◽  
Thomas Hennessy ◽  
David Bradley ◽  
Berit Ebert ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Stress Responses ◽  
Lipid Analysis ◽  
Lipid Extraction ◽  
Extraction Methods ◽  
Single Step ◽  
Lipid Classes ◽  
Model Plant Arabidopsis Thaliana ◽  
Extraction Period ◽  
Different Tissues

Abstract Background: The plant lipidome is highly complex, and the composition of lipids in different tissues as well as their specific functions in plant development, growth and stress responses have yet to be fully elucidated. To do this, efficient lipid extraction protocols which deliver target compounds in solution at concentrations adequate for subsequent detection, quantitation and analysis through spectroscopic methods are required. To date, numerous methods are used to extract lipids from plant tissues. However, a comprehensive analysis of the efficiency and reproducibility of these methods to extract multiple lipid classes from diverse tissues of a plant has not been undertaken. Results: In this study, we report the comparison of four different lipid extraction procedures in order to determine the most effective lipid extraction protocol to extract lipids from different tissues of the model plant Arabidopsis thaliana . Conclusion: While particular methods were best suited to extract different lipid classes from diverse Arabidopsis tissues, overall a single-step extraction method with a 24 h extraction period, which uses a mixture of chloroform, isopropanol, methanol and water, was the most efficient, reproducible and the least labor-intensive to extract a broad range of lipids for untargeted lipidomic analysis of Arabidopsis tissues. This method extracted a broad range of lipids from leaves, stems, siliques, roots, seeds, seedlings and flowers of Arabidopsis. In addition, appropriate methods for targeted lipid analysis of specific lipids from particular Arabidopsis tissues were also identified.

scholarly journals A comparison of different lipid extraction methods for different tissue types of Arabidopsis thaliana

2020 ◽  
Author(s):  
Cheka Kehelpannala ◽  
Thusitha Wasantha Thilaka Rupasinghe ◽  
Thomas Hennessy ◽  
David Bradley ◽  
Berit Ebert ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Stress Responses ◽  
Lipid Analysis ◽  
Lipid Extraction ◽  
Extraction Methods ◽  
Single Step ◽  
Lipid Classes ◽  
Specific Lipids ◽  
Extraction Period ◽  
Different Tissues

Abstract Background: The plant lipidome is highly complex, and the composition of lipids in different tissues as well as their specific functions in plant development, growth and stress responses have yet to be fully elucidated. To do this, efficient lipid extraction protocols which deliver target compounds in solution at concentrations adequate for subsequent detection, quantitation and analysis through spectroscopic methods are required. To date, numerous methods are used to extract lipids from plant tissues. However, a comprehensive analysis of the efficiency and reproducibility of these methods to extract multiple lipid classes from diverse tissues of a plant has not been undertaken. Results: In this study, we report the comparison of four different lipid extraction procedures in order to determine the most effective lipid extraction protocol to extract lipids from different tissues of the model plant Arabidopsis thaliana. Conclusion: While particular methods were best suited to extract different lipid classes from diverse Arabidopsis tissues, overall a single-step extraction method with a 24 h extraction period, which uses a mixture of chloroform, isopropanol, methanol and water, was the most efficient, reproducible and the least labor-intensive to extract a broad range of lipids for untargeted lipidomic analysis of Arabidopsis tissues. This method extracted a broad range of lipids from leaves, stems, siliques, roots, seeds, seedlings and flowers of Arabidopsis. In addition, appropriate methods for targeted lipid analysis of specific lipids from particular Arabidopsis tissues were also identified.

scholarly journals A comprehensive comparison of four methods for extracting lipids from Arabidopsis tissues

Plant Methods ◽  
2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Cheka Kehelpannala ◽  
Thusitha W. T. Rupasinghe ◽  
Thomas Hennessy ◽  
David Bradley ◽  
Berit Ebert ◽  
...  
Keyword(s):  
Stress Responses ◽  
Lipid Analysis ◽  
Lipid Extraction ◽  
Single Step ◽  
Lipid Classes ◽  
Plant Tissues ◽  
Specific Lipids ◽  
Comprehensive Comparison ◽  
Extraction Period ◽  
Different Tissues

Abstract Background The plant lipidome is highly complex, and the composition of lipids in different tissues as well as their specific functions in plant development, growth and stress responses have yet to be fully elucidated. To do this, efficient lipid extraction protocols which deliver target compounds in solution at concentrations adequate for subsequent detection, quantitation and analysis through spectroscopic methods are required. To date, numerous methods are used to extract lipids from plant tissues. However, a comprehensive analysis of the efficiency and reproducibility of these methods to extract multiple lipid classes from diverse tissues of a plant has not been undertaken. Results In this study, we report the comparison of four different lipid extraction procedures in order to determine the most effective lipid extraction protocol to extract lipids from different tissues of the model plant Arabidopsis thaliana. Conclusion While particular methods were best suited to extract different lipid classes from diverse Arabidopsis tissues, overall a single-step extraction method with a 24 h extraction period, which uses a mixture of chloroform, isopropanol, methanol and water, was the most efficient, reproducible and the least labor-intensive to extract a broad range of lipids for untargeted lipidomic analysis of Arabidopsis tissues. This method extracted a broad range of lipids from leaves, stems, siliques, roots, seeds, seedlings and flowers of Arabidopsis. In addition, appropriate methods for targeted lipid analysis of specific lipids from particular Arabidopsis tissues were also identified.

scholarly journals Single-Step Extraction Coupled with Targeted HILIC-MS/MS Approach for Comprehensive Analysis of Human Plasma Lipidome and Polar Metabolome

Metabolites ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 495
Author(s):  
Jessica Medina ◽  
Vera van der Velpen ◽  
Tony Teav ◽  
Yann Guitton ◽  
Hector Gallart-Ayala ◽  
...  
Keyword(s):  
Human Plasma ◽  
Large Scale ◽  
Lipid Extraction ◽  
Single Step ◽  
Methyl Tert Butyl Ether ◽  
Method Performance ◽  
Simultaneous Extraction ◽  
Lipid Species ◽  
Polar Metabolites ◽  
Complex Lipids

Expanding metabolome coverage to include complex lipids and polar metabolites is essential in the generation of well-founded hypotheses in biological assays. Traditionally, lipid extraction is performed by liquid-liquid extraction using either methyl-tert-butyl ether (MTBE) or chloroform, and polar metabolite extraction using methanol. Here, we evaluated the performance of single-step sample preparation methods for simultaneous extraction of the complex lipidome and polar metabolome from human plasma. The method performance was evaluated using high-coverage Hydrophilic Interaction Liquid Chromatography-ESI coupled to tandem mass spectrometry (HILIC-ESI-MS/MS) methodology targeting a panel of 1159 lipids and 374 polar metabolites. The criteria used for method evaluation comprised protein precipitation efficiency, and relative MS signal abundance and repeatability of detectable lipid and polar metabolites in human plasma. Among the tested methods, the isopropanol (IPA) and 1-butanol:methanol (BUME) mixtures were selected as the best compromises for the simultaneous extraction of complex lipids and polar metabolites, allowing for the detection of 584 lipid species and 116 polar metabolites. The extraction with IPA showed the greatest reproducibility with the highest number of lipid species detected with the coefficient of variation (CV) < 30%. Besides this difference, both IPA and BUME allowed for the high-throughput extraction and reproducible measurement of a large panel of complex lipids and polar metabolites, thus warranting their application in large-scale human population studies.

scholarly journals Hybrid liquid biphasic system for cell disruption and simultaneous lipid extraction from microalgae Chlorella sorokiniana CY-1 for biofuel production

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Guo Yong Yew ◽  
Kit Wayne Chew ◽  
Marlinda Abdul Malek ◽  
Yeek-Chia Ho ◽  
Wei-Hsin Chen ◽  
...  
Keyword(s):  
Cell Wall ◽  
Organic Solvent ◽  
Lipid Extraction ◽  
Research Work ◽  
Volume Ratio ◽  
Single Step ◽  
Biofuel Production ◽  
Biphasic System ◽  
One Step ◽  
Extraction Processes

Abstract Background The extraction of lipids from microalgae requires a pretreatment process to break the cell wall and subsequent extraction processes to obtain the lipids for biofuels production. The multistep operation tends to incur high costs and are energy intensive due to longer process operations. This research work applies the combination of radicals from hydrogen peroxide with an organic solvent as a chemical pretreatment method for disrupting the cell wall of microalgae and simultaneously extracting lipids from the biomass in a one-step biphasic solution. Result Several parameters which can affect the biphasic system were analyzed: contact time, volume of solvent, volume ratio, type of organic solvent, biomass amount and concentration of solvents, to extract the highest amount of lipids from microalgae. The results were optimized and up to 83.5% of lipid recovery yield and 94.6% of enhancement was successfully achieved. The results obtain from GC-FID were similar to the analysis of triglyceride lipid standard. Conclusion The profound hybrid biphasic system shows great potential to radically disrupt the cell wall of microalgae and instantaneously extract lipids in a single-step approach. The lipids extracted were tested to for its comparability to biodiesel performance.

scholarly journals Advances in Lipid Extraction Methods—A Review

2021 ◽  
Vol 22 (24) ◽  
pp. 13643
Author(s):  
Ramesh Kumar Saini ◽  
Parchuri Prasad ◽  
Xiaomin Shang ◽  
Young-Soo Keum
Keyword(s):  
Lipid Analysis ◽  
Neutral Lipids ◽  
Lipid Extraction ◽  
Critical Factor ◽  
Biological Tissues ◽  
Extraction Methods ◽  
Single Step ◽  
Green Solvents ◽  
Advantages And Disadvantages ◽  
Environmentally Safe

Extraction of lipids from biological tissues is a crucial step in lipid analysis. The selection of appropriate solvent is the most critical factor in the efficient extraction of lipids. A mixture of polar (to disrupt the protein-lipid complexes) and nonpolar (to dissolve the neutral lipids) solvents are precisely selected to extract lipids efficiently. In addition, the disintegration of complex and rigid cell-wall of plants, fungi, and microalgal cells by various mechanical, chemical, and enzymatic treatments facilitate the solvent penetration and extraction of lipids. This review discusses the chloroform/methanol-based classical lipid extraction methods and modern modifications of these methods in terms of using healthy and environmentally safe solvents and rapid single-step extraction. At the same time, some adaptations were made to recover the specific lipids. In addition, the high throughput lipid extraction methodologies used for liquid chromatography-mass spectrometry (LC-MS)-based plant and animal lipidomics were discussed. The advantages and disadvantages of various pretreatments and extraction methods were also illustrated. Moreover, the emerging green solvents-based lipid extraction method, including supercritical CO2 extraction (SCE), is also discussed.

scholarly journals Microalgal Lipid Extraction and Evaluation of Single-Step Biodiesel Production

2012 ◽  
Vol 16 (5) ◽  
pp. 157-166 ◽  
Author(s):  
Chattip Prommuak ◽  
Prasert Pavasant ◽  
Armando T Quitain ◽  
Motonobu Goto ◽  
Artiwan Shotipruk
Keyword(s):  
Lipid Extraction ◽  
Single Step ◽  
Biodiesel Production

scholarly journals A High-Throughput Method (ASE-GC/MS/MS/MRM) for Quantification of Multiple Hydrocarbon Compounds in Marine Environmental Samples

2018 ◽  
Vol 52 (6) ◽  
pp. 66-70 ◽  
Author(s):  
Isabel C. Romero
Keyword(s):  
High Throughput ◽  
Environmental Samples ◽  
Multiple Reaction Monitoring ◽  
Lipid Extraction ◽  
Single Step ◽  
Oxidation Products ◽  
Extraction And Purification ◽  
High Throughput Method ◽  
Multiple Reaction Monitoring Mode ◽  
Polycyclic Aromatic

AbstractA high-throughput method was applied in small and complex environmental samples for targeting multiple organic fractions (polar, nonpolar). The analytical method consists of a single-step lipid extraction and purification procedure (accelerated solvent extraction [ASE] system) coupled with a single-run step using gas chromatography with tandem mass spectrometry, operated in multiple reaction monitoring mode (GC/MS/MS/MRM). Successful application of this method is summarized for multiple chemical groups (aliphatics, terpanes, steranes, triaromatic steroids, polycyclic aromatic hydrocarbons, and oxidation products).

An Improved, Single Step Standardized Method of Lipid Extraction from Human Skeletal Muscle Tissue

2006 ◽  
Vol 39 (2) ◽  
pp. 297-315 ◽  
Author(s):  
Niraj Kumar Srivastava ◽  
Sunil Pradhan ◽  
Balraj Mittal ◽  
Raj Kumar ◽  
G. A. Nagana Gowda
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Human Skeletal Muscle ◽  
Lipid Extraction ◽  
Single Step ◽  
Skeletal Muscle Tissue ◽  
Standardized Method

A Resorcinol-Formaldehyde Resin as an Embedding Material for Electron Microscopy of Membranes

1969 ◽  
Vol 27 ◽  
pp. 328-329 ◽  
Author(s):  
J. G. Robertson ◽  
D. F. Parsons
Keyword(s):  
Electron Microscopy ◽  
Osmium Tetroxide ◽  
Neutral Lipids ◽  
Lipid Extraction ◽  
Water Soluble ◽  
Formaldehyde Resin ◽  
Electron Microscope Autoradiography ◽  
Resorcinol Formaldehyde ◽  
Major Disadvantage ◽  
Cell Organization

The extraction of lipids from tissues during fixation and embedding for electron microscopy is widely recognized as a source of possible artifact, especially at the membrane level of cell organization. Lipid extraction is also a major disadvantage in electron microscope autoradiography of radioactive lipids, as in studies of the uptake of radioactive fatty acids by intestinal slices. Retention of lipids by fixation with osmium tetroxide is generally limited to glycolipids, phospholipids and highly unsaturated neutral lipids. Saturated neutral lipids and sterols tend to be easily extracted by organic dehydrating reagents prior to embedding. Retention of the more saturated lipids in embedded tissue might be achieved by developing new cross-linking reagents, by the use of highly water soluble embedding materials or by working at very low temperatures.

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