Analysis of Th1/Th2 response pattern with Treg cell inhibition and stochastic effect

Background. Regulatory T cells (Tregs) are important in regulating responses to innocuous antigens, such as allergens, by controlling the Th2 response, a mechanism that appears to be compromised in atopic asthmatic individuals. Different isogenic mouse strains also have distinct immunological responses and susceptibility to the experimental protocols used to develop lung allergic inflammation. In this work, we investigated the differences in the frequency of Treg cell subtypes among A/J, BALB/c, and C57BL/6, under normal conditions and following induction of allergic asthma with ovalbumin (OVA). Methods. Subcutaneous sensitization followed by 4 consecutive intranasal OVA challenges induced asthma characteristic changes such as airway hyperreactivity, inflammation, and production of Th2 cytokines (IL-4, IL-13, IL-5, and IL-33) in the lungs of only A/J and BALB/c but not C57BL/6 strain and evaluated by invasive whole-body plethysmography, flow cytometry, and ELISA, respectively. Results. A/J strain naturally showed a higher frequency of CD4+IL-10+ T cells in the lungs of naïve mice compared to the other strains, accompanied by higher frequencies of CD4+IL-4+ T cells. C57BL/6 mice did not develop lung inflammation and presented higher frequency of CD4+CD25+Foxp3+ Treg cells in the bronchoalveolar lavage fluid (BALF) after the allergen challenge. In in vitro settings, allergen-specific stimulation of mediastinal LN (mLN) cells from OVA-challenged animals induced higher frequency of CD4+IL-10+ Treg cells from A/J strain and CD4+CD25+Foxp3+ from C57BL/6. Conclusions. The observed differences in the frequencies of Treg cell subtypes associated with the susceptibility of the animals to experimental asthma suggest that CD4+CD25+Foxp3+ and IL-10-producing CD4+ Treg cells may play different roles in asthma control. Similar to asthmatic individuals, the lack of an efficient regulatory response and susceptibility to the development of experimental asthma in A/J mice further suggests that this strain could be preferably chosen in experimental models of allergic asthma.

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Analysis of Th1/Th2 response pattern for erythrodermic psoriasis

Journal of Huazhong University of Science and Technology [Medical Sciences] ◽

10.1007/s11596-014-1322-0 ◽

2014 ◽

Vol 34 (4) ◽

pp. 596-601 ◽

Cited By ~ 13

Author(s):

Ping Zhang ◽

Hong-xiang Chen ◽

Yi-qun Duan ◽

Wei-zhen Wang ◽

Tian-zhu Zhang ◽

...

Keyword(s):

Response Pattern ◽

Th2 Response ◽

Erythrodermic Psoriasis

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Stimulus Ratio Effects on Speech Discrimination by Children and Adults

Journal of Speech Language and Hearing Research ◽

10.1044/jshr.3403.671 ◽

1991 ◽

Vol 34 (3) ◽

pp. 671-678 ◽

Cited By ~ 9

Author(s):

Joan E. Sussman

Keyword(s):

Discrimination Task ◽

Response Pattern ◽

Response Patterns ◽

Speech Discrimination ◽

Adult Group ◽

Discrimination Accuracy ◽

Formant Frequency ◽

Response Strategies ◽

Place Of Articulation ◽

Adults And Children

This investigation examined the response strategies and discrimination accuracy of adults and children aged 5–10 as the ratio of same to different trials was varied across three conditions of a “change/no-change” discrimination task. The conditions varied as follows: (a) a ratio of one-third same to two-thirds different trials (33% same), (b) an equal ratio of same to different trials (50% same), and (c) a ratio of two-thirds same to one-third different trials (67% same). Stimuli were synthetic consonant-vowel syllables that changed along a place of articulation dimension by formant frequency transition. Results showed that all subjects changed their response strategies depending on the ratio of same-to-different trials. The most lax response pattern was observed for the 50% same condition, and the most conservative pattern was observed for the 67% same condition. Adult response patterns were most conservative across condition. Differences in discrimination accuracy as measured by P(C) were found, with the largest difference in the 5- to 6-year-old group and the smallest change in the adult group. These findings suggest that children’s response strategies, like those of adults, can be manipulated by changing the ratio of same-to-different trials. Furthermore, interpretation of sensitivity measures must be referenced to task variables such as the ratio of same-to-different trials.

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Maternal Perception of Infants’ Expressions of Emotion

Journal of Psychophysiology ◽

10.1027/0269-8803/a000008 ◽

2010 ◽

Vol 24 (3) ◽

pp. 173-185 ◽

Cited By ~ 6

Author(s):

Martin Krippl ◽

Stephanie Ast-Scheitenberger ◽

Ina Bovenschen ◽

Gottfried Spangler

Keyword(s):

Response Pattern ◽

Electrodermal Activity ◽

Startle Amplitude ◽

Caregiving System ◽

Parental Caregiving ◽

Opponent Process ◽

Opponent Process Theory ◽

Valence And Arousal ◽

Methodological Considerations

In light of Lang’s differentiation of the aversive and the approach system – and assumptions stemming from attachment theory – this study investigates the role of the approach or caregiving system for processing infant emotional stimuli by comparing IAPS pictures, infant pictures, and videos. IAPS pictures, infant pictures, and infant videos of positive, neutral, or negative content were presented to 69 mothers, accompanied by randomized startle probes. The assessment of emotional responses included subjective ratings of valence and arousal, corrugator activity, the startle amplitude, and electrodermal activity. In line with Lang’s original conception, the typical startle response pattern was found for IAPS pictures, whereas no startle modulation was observed for infant pictures. Moreover, the startle amplitudes during negative video scenes depicting crying infants were reduced. The results are discussed with respect to several theoretical and methodological considerations, including Lang’s theory, emotion regulation, opponent process theory, and the parental caregiving system.

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Confound It!

European Journal of Psychological Assessment ◽

10.1027/1015-5759/a000459 ◽

2019 ◽

Vol 35 (6) ◽

pp. 855-867 ◽

Cited By ~ 2

Author(s):

John T. Kulas ◽

Rachael Klahr ◽

Lindsey Knights

Keyword(s):

Social Desirability ◽

Response Pattern ◽

Experimental Manipulation ◽

Scoring Method ◽

Method Effect ◽

Coding Method ◽

The Social ◽

Current Article ◽

Method Factors ◽

Psychological Inventory

Abstract. Many investigators have noted “reverse-coding” method factors when exploring response pattern structure with psychological inventory data. The current article probes for the existence of a confound in these investigations, whereby an item’s level of saturation with socially desirable content tends to covary with the item’s substantive scale keying. We first investigate its existence, demonstrating that 15 of 16 measures that have been previously implicated as exhibiting a reverse-scoring method effect can also be reasonably characterized as exhibiting a scoring key/social desirability confound. A second set of analyses targets the extent to which the confounding variable may confuse interpretation of factor analytic results and documents strong social desirability associations. The results suggest that assessment developers perhaps consider the social desirability scale value of indicators when constructing scale aggregates (and possibly scales when investigating inter-construct associations). Future investigations would ideally disentangle the confound via experimental manipulation.

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