Distribution characteristics and potential risks of heavy metals and antimicrobial resistant Escherichia coli in dairy farm wastewater in Tai’an, China

Chemosphere ◽  
2021 ◽  
Vol 262 ◽  
pp. 127768
Author(s):  
Cong Liu ◽  
Yu Liu ◽  
Chenglian Feng ◽  
Peng Wang ◽  
Lanping Yu ◽  
...  
2021 ◽  
Vol 19 (3) ◽  
pp. 349-362
Author(s):  
Prayuth Saekhow ◽  
◽  
Chayaphon Sriphannam ◽  
◽  

We investigated the prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains in dairy farm wastewater in Chiang Mai, Thailand. We analyzed wastewater samples collected from 150 dairy farms and found that 88.7% of the farms (n = 133) were positive for ESBL-producing E. coli. Multiplex polymerase chain reaction (PCR) amplification was performed to characterize the presence of bla CTX-M, bla TEM, and blaSHV in ESBL-producing isolates. blaCTX-M was found in all isolates (n = 133), followed by blaTEM (80/133, 60.2%), whereas blaSHV was not detected in any isolate. blaCTX-M and blaTEM were present in 60.2% (80/133) of the isolates, and 39.8% (53/133) isolates carried bla CTX-M alone. Subgroup analysis showed that CTX-M-1 was the most prevalent subgroup among the isolates (129/133, 97.0%), followed by CTX-M-8 (2/133, 1.5%) and CTX-M-9 (2/133, 1.5%). The distribution of the phylogenetic groups was as follows: group A (100/133, 75.2%), followed by B1 (14/133, 10.5%), D (6/133, 4.5%), F (6/133, 4.5%), B2 (4/133, 3.0%), and E (3/133, 2.3%). Based on enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and dendrogram analysis, 24 isolates were classified into clades I (n = 21), II (n =1), and III (n =2). Minor genetic differences were found in all clade I isolates. Our data suggest that the circulating of ESBL-producing E. coli carried at least one bla gene strain distributed in dairy farm wastewater in Chiang Mai.


2019 ◽  
Vol 12 (7) ◽  
pp. 984-993 ◽  
Author(s):  
Md. Abdus Sobur ◽  
Abdullah Al Momen Sabuj ◽  
Ripon Sarker ◽  
A. M. M. Taufiqur Rahman ◽  
S. M. Lutful Kabir ◽  
...  

Aim: The present study was carried out to determine load of total bacteria, Escherichia coli and Salmonella spp. in dairy farm and its environmental components. In addition, the antibiogram profile of the isolated bacteria having public health impact was also determined along with identification of virulence and resistance genes by polymerase chain reaction (PCR) under a one-health approach. Materials and Methods: A total of 240 samples of six types (cow dung - 15, milk - 10, milkers' hand wash - 10, soil - 10 water - 5, and vegetables - 10) were collected from four dairy farms. For enumeration, the samples were cultured onto plate count agar, eosin methylene blue, and xylose-lysine deoxycholate agar and the isolation and identification of the E. coli and Salmonella spp. were performed based on morphology, cultural, staining, and biochemical properties followed by PCR. The pathogenic strains of E. coli stx1, stx2, and rfbO157 were also identified through PCR. The isolates were subjected to antimicrobial susceptibility test against 12 commonly used antibiotics by disk diffusion method. Detection of antibiotic resistance genes ereA, tetA, tetB, and SHV were performed by PCR. Results: The mean total bacterial count, E. coli and Salmonella spp. count in the samples ranged from 4.54±0.05 to 8.65±0.06, 3.62±0.07 to 7.04±0.48, and 2.52±0.08 to 5.87±0.05 log colony-forming unit/g or ml, respectively. Out of 240 samples, 180 (75%) isolates of E. coli and 136 (56.67%) isolates of Salmonella spp. were recovered through cultural and molecular tests. Among the 180 E. coli isolates, 47 (26.11%) were found positive for the presence of all the three virulent genes, of which stx1 was the most prevalent (13.33%). Only three isolates were identified as enterohemorrhagic E. coli. Antibiotic sensitivity test revealed that both E. coli and Salmonella spp. were found highly resistant to azithromycin, tetracycline, erythromycin, oxytetracycline, and ertapenem and susceptible to gentamycin, ciprofloxacin, and imipenem. Among the four antibiotic resistance genes, the most observable was tetA (80.51-84.74%) in E. coli and Salmonella spp. and SHV genes were the lowest one (22.06-25%). Conclusion: Dairy farm and their environmental components carry antibiotic-resistant pathogenic E. coli and Salmonella spp. that are potential threat for human health which requires a one-health approach to combat the threat.


Irriga ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 590-602
Author(s):  
Jonathas Batista Goncalves Silva ◽  
Mauro Aparecido Martinez ◽  
Luiz Carlos Chamhum Salomão ◽  
Paulo Roberto Cecon ◽  
Antonio Teixeira de Matos ◽  
...  

EFFECTS OF DAIRY FARM WASTEWATER USE IN CULTIVATION ON FIG TREE (FICUS CARICA L.)     JONATHAS BATISTA GONCALVES SILVA1; MAURO APARECIDO MARTINEZ2; LUIZ CARLOS CHAMHUM SALOMÃO3; PAULO ROBERTO CECON4; ANTONIO TEIXEIRA DE MATOS5 E LEONARDO DUARTE BATISTA DA SILVA6   1Departamento de Engenharia Sanitária e Ambiental, UFJF, Rua José Lourenço Kelmer, s/n,Bairro São Pedro, 36036-900, Juiz de Fora, MG, Brasil, [email protected]. 2Departamento de Engenharia Agrícola, UFV, Av. Peter Henry Rolfs, s/n, 36570-900, Viçosa, MG, Brasil, [email protected] 3Departamento de Fitotecnia, UFV, Av. Peter Henry Rolfs, s/n, 36570-900, Viçosa, MG, Brasil, [email protected] 4Departamento de Estatística, UFV, Av. Peter Henry Rolfs, s/n, 36570-900, Viçosa, MG, Brasil,[email protected]. 5Departamento de Engenharia Sanitária e Ambiental, UFMG, Avenida Presidente Antônio Carlos, 6627, Bairro Pampulha,31270-901, Belo Horizonte, MG, Brasil,[email protected] 6Departamento de Engenharia, UFRRJ, Rodovia BR 465,km 7, s/n,23890-000, Seropédica, RJ, Brasil, [email protected]     1 ABSTRACT   The purpose of this study was to evaluate the effects of dairy farm wastewater (DFW) use on fig tree growth, production, on fig health standard and on nutrient concentration in fig tree leaves. The study was developed in the Integrated Agroecological System area, in Seropédica (RJ, Brazil) between June  2011 and May  2012. The applied fertilizer formulations were: Formulation 1, 100% of nitrogen dose recommended for fig tree supplied by fertilizing with castor bean cake (CB); Formulation 2, 50% of nitrogen dose supplied by DFW application and 50% of nitrogen dose supplied by CB; Formulation 3, 75% of nitrogen dose supplied by DFW application and 25% of nitrogen dose from CB; Formulation 4, 100% of nitrogen dose supplied by DFW application.Data were submitted to analysis of variance and the averages were compared by Tukey’s test at 10% probability. The results demonstrated that branches length, number of leaves per branch, number of fruits, production and yield were lower in plants submitted to Formulation 4. Contamination of fruits by thermotolerant coliforms or Salmonellasp did not occur after DFW use as fertilizer. The results showed that the use of DFW in fig tree cultivation was sufficient to provide the nutritional needs of plants, as regards macronutrients and Fe.   Keywords: fertirrigation, environmental impact, final disposal of effluents and crop nutrition.     SILVA, J.B. G.; MARTINEZ, M. A.; SALOMÃO, L. C. C.; CECON,P. R.; MATOS, A. T.; SILVA, L. D. B. EFEITOS DO USO DE ÁGUA RESIDUÁRIA DE BOVINOCULTURANO CULTIVO DA FIGUEIRA (FICUS CARICA L.)         2 RESUMO   Objetivou-se com este trabalho avaliar os efeitos do uso da água residuária de bovinocultura de leite (ARB) no crescimento, produção, padrão fitossanitário dos frutos e na concentração de nutrientes nas folhas da figueira. As formulações de adubação aplicadas foram: Adubação 1 - 100% da dose de nitrogênio fornecida pela adubação com torta de mamona (TM); Adubação 2 - 50% da dose de nitrogênio comaplicação de ARB e 50% com TM; Adubação 3 - 75% da dose de nitrogênio com aplicação de ARB e os outros 25% da dose com TM; Adubação 4 - 100% da dose de nitrogênio com aplicação da ARB. Os resultados foram submetidos à análise de variância e as médias comparadas utilizando-se o Teste de Tukey a 10% de probabilidade. Diante dos resultados verificou-se que comprimento dos ramos, o número de folhas por ramos, o número de frutos, a produção e a produtividade foram menores nas plantas submetidas à Adubação4. Não ocorreu contaminação dos frutos por coliformes termotolerantes e Salmonella sp. Diante dos resultados concluiu-se que o uso de ARB no cultivo da figueira não proporciona deficiência nutricional às plantas no que se refere aos macronutrientes (N, Ca, Mg, K e P).   Palavras-chave: fertirrigação, impacto ambiental, disposição final de efluentes, nutrição vegetal.


2014 ◽  
Vol 675-677 ◽  
pp. 299-304
Author(s):  
Ren Jun Liang ◽  
Xiao Tian Ma ◽  
Ji Cai Qiu

This study assessed the ecological risk associated with heavy metals in surface sediments in a specific setting: the Wuhan wetland. Metals were extracted and characterized using the Tessier method, and the morphological distribution characteristics and correlation of Zn, Mn, Fe, Cr, and As were analyzed. The results indicated that heavy metals at the study site mainly exist in residual state forms; the Mn pollution was serious; the potential risk from Cr and As was larger; and the environmental risk from Fe and Zn was lower. In order of potential ecological harm: As > Zn > Cr > Mn. The overall risk of ecological harm was slight, but the individual region has seen moderate ecological damage because of As pollution. Because this element has a significant toxicity level, it is important to remain attentive to the pollution caused by it.


2010 ◽  
Author(s):  
Anthony E Salvucci ◽  
Mara Elton ◽  
Larry D Geohring ◽  
Brian K Richards ◽  
Anthony G Hay ◽  
...  

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