Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater

Chemosphere ◽  
2021 ◽  
pp. 131266
Author(s):  
Junyuan Guo ◽  
Cheng Chen ◽  
Wenjing Chen ◽  
Jianying Jiang ◽  
Bozhi Chen ◽  
...  
Author(s):  
Castañeda Alvarez Estefania ◽  
Sánchez Leal Ligia

For farmers the use of agrochemicals is the preferred method to control pests and diseases. Considering the market demand for biological control products, the encapsulation could be a competent alternative to current commercial formulations for cellular viability and controlled release. The purpose of this study was to use ionic gelation with sodium alginate, starch and maltodextrin to immobilize Bacillus subtilis and to evaluate the biocontrol effect against Fusarium oxysporum f. sp. lycopersici in vitro. The matrix with a concentration of 2% sodium alginate, 1% starch, and 1% maltodextrin is a suitable method for cellular viability and biological control activity against Fusarium oxysporum f. sp. lycopersici, with a reduction of mycelial growth of 49.6% and a survival rate for Bacillus subtilis of 98.05% (p less than 0.0001).The use of immobilized bacteria as biological control agents are sustainable and effective bio-inputs that could be used at industrial scale and benefit the tomato crops against attack by Fusarium oxysporum f. sp. lycopersici.


Membranes ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 594
Author(s):  
Run-Feng Chen ◽  
Tao Liu ◽  
Hong-Wei Rong ◽  
Hai-Tao Zhong ◽  
Chun-Hai Wei

Anaerobically treated swine wastewater contains large amounts of orthophosphate phosphorus, ammonium nitrogen and organic substances with potential nutrients recovery via struvite electrochemical precipitation post-treatment. Lab-scale batch experiments were systematically conducted in this study to investigate the effects of initial pH, current density, organic substances upon nutrients removal, and precipitates quality (characterized by X-ray diffraction, scanning electron microscopy and element analysis via acid dissolution method) during the struvite electrochemical precipitation process. The optimal conditions for the initial pH of 7.0 and current density of 4 mA/cm2 favoured nutrients removal and precipitates quality (struvite purity of up to 94.2%) in the absence of organic substances. By contrast, a more adverse effect on nutrients removal, morphology and purity of precipitates was found by humic acid than by sodium alginate and bovine albumin in the individual presence of organic substances. Low concentration combination of bovine albumin, sodium alginate, and humic acid showed antagonistic inhibition effects, whereas a high concentration combination showed the accelerating inhibition effects. Initial pH adjustment from 7 to 8 could effectively mitigate the adverse effects on struvite electrochemical precipitation under high concentration combined with organic substances (500 mg/L bovine albumin, 500 mg/L sodium alginate, and 1500 mg/L humic acid); this may help improve struvite electrochemical precipitation technology in practical application for nutrients recovery from anaerobically treated swine wastewater.


2011 ◽  
Vol 356-360 ◽  
pp. 1469-1474
Author(s):  
Jun Jing Guo ◽  
Wen Bin Chen

In this study, calcium alginate immobilized bacillus subtilis body was used as an adsorbent for the aim of adsorpting Cu(Ⅱ) ions form the aqueous solutions, On sutiable condition, this study use purely bacteria eliminate other bacteria come in life-form bacteria .Through the orthogonal experiment obtains the optimum condition of sodium alginate fossilization bacillus subtilus pellet to the Cu(Ⅱ) adsorption, The biosorption efficiency was strongly influenced by the pH and the initial concentration ofthe Cu(Ⅱ) aqueous solutions. We also discusses the influence of single factor to the sodium alginate fossilization bacillus subtilus pellet to the Cu(Ⅱ) adsorption ,which included pH, contact time, temperature, initial metal ion concentrations and ionic strength in the adsorption process. The adsorption of the adsorbent on Cu(Ⅱ) is related to its concentration . From the research,we know the maximum adsorption rate to the Cu(Ⅱ) is about 83.2%. Using different model to analyse data. The sorption process follows pseudo-second-order kinetics very well,and the correlation coefficients R all reached to 0.9, and the rate constant of this kinetics model for the adsorption processes, k2, increased with the increasing temperatures. The isothermal data could be well described by the Langmuir adsorption isothermal equations.The experimental results showed that heavy metals could be eficciently accumulated by the immobilized bacteria.


2005 ◽  
Vol 71 (12) ◽  
pp. 8565-8572 ◽  
Author(s):  
Cesar Mota ◽  
Melanie A. Head ◽  
Jennifer A. Ridenoure ◽  
Jay J. Cheng ◽  
Francis L. de los Reyes

ABSTRACT The effects of the lengths of aeration and nonaeration periods on nitrogen removal and the nitrifying bacterial community structure were assessed in intermittently aerated (IA) reactors treating digested swine wastewater. Five IA reactors were operated in parallel with different aeration-to-nonaeration time ratios (ANA). Populations of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) were monitored using 16S rRNA slot blot hybridizations. AOB species diversity was assessed using amoA gene denaturant gradient gel electrophoresis. Nitrosomonas and Nitrosococcus mobilis were the dominant AOB and Nitrospira spp. were the dominant NOB in all reactors, although Nitrosospira and Nitrobacter were also detected at lower levels. Reactors operated with the shortest aeration time (30 min) showed the highest Nitrosospira rRNA levels, and reactors operated with the longest anoxic periods (3 and 4 h) showed the lowest levels of Nitrobacter, compared to the other reactors. Nitrosomonas sp. strain Nm107 was detected in all reactors, regardless of the reactor's performance. Close relatives of Nitrosomonas europaea, Nitrosomonas sp. strain ENI-11, and Nitrosospira multiformis were occasionally detected in all reactors. Biomass fractions of AOB and effluent ammonia concentrations were not significantly different among the reactors. NOB were more sensitive than AOB to long nonaeration periods, as nitrite accumulation and lower total NOB rRNA levels were observed for an ANA of 1 h:4 h. The reactor with the longest nonaeration time of 4 h performed partial nitrification, followed by denitrification via nitrite, whereas the other reactors removed nitrogen through traditional nitrification and denitrification via nitrate. Superior ammonia removal efficiencies were not associated with levels of specific AOB species or with higher AOB species diversity.


2011 ◽  
Vol 236-238 ◽  
pp. 2371-2377 ◽  
Author(s):  
Yan Wang ◽  
Chen Lu Zhang ◽  
Ping Li ◽  
Pei Gen Zhou ◽  
Xiao Rong Pan

Sodium alginate and cellulose were combined to use as a composite carrier forPseudomonassp. CUY8 chitosanase immobilization. For free enzyme, immobilized chitosanase within different carriers of sodium alginate and composite carrier, Km values were 1.919, 9.27, and 5.91µM, respectively. The increase of Km value of immobilized chitosanase with composite carrier was lower than that of single carrier. This indicates that the composite carrier of sodium alginate/ cellulose improves the affinity of chitosanase to the substrate. Furthermore, chitosanase immobilization using composite carrier shows improved thermal stability ranging from 65 to 80°C, and enzyme residual activities were more than 75%. The effects of ratio of enzyme to substrate on chitooligosaccharides (COS) production were determined, and COS yields with composite carrier was 68% at optimum ratio of 1:1. Since the immobilization process using composite carrier is simple and effective, this method could be used for the industrial production of COS.


1997 ◽  
Vol 35 (8) ◽  
pp. 169-175 ◽  
Author(s):  
Lei Yang

A new biotechnology of immobilization of nitrifying bacteria is developed to improve the effect of nitrification, in which a concentrated enriched culture of nitrifiers is entrapped into a special biomass carrier and immobilized by sodium alginate to form spherical pellets with diameters ranged from 1 to 2 mm. In addition, zeolite, which is a natural ion exchanger of ammonium, was co-immobilized into the pellets to enhance the efficiencies of transferring ammonium into the pellets as nitrifiers' substrate. The system was operated under five different initial applied ammonia concentration levels. The experimental results showed that physical air stripping effect was stronger than both chemical ion exchange and biological nitrification effects occurring in the system applied with initial ammonia concentration levels of both 10 and 20 mg N/I. In the experiments applied with initial ammonia concentration levels of 50, 70, and 100 mg N/I to the system, due to high nitrite concentrations detected in the effluents, it was inferred that both ion exchange and nitrification effects were dominant over air stripping effect. The reasons might be due to higher ammonium concentrations existing in the system causing stronger effects of ion exchange by zeolite entrapped inside the pellets. Hence, this new ammonia removal biotechnology appeared to be suitable to treat the wastewaters containing higher ammonia concentrations.


Energies ◽  
2020 ◽  
Vol 13 (13) ◽  
pp. 3413
Author(s):  
Adele Folino ◽  
Paolo Salvatore Calabrò ◽  
Demetrio Antonio Zema

In order to overcome anaerobic digestion (AD) inhibition due to the large nitrogen content of swine wastewater (SW), air stripping (AS) and other chemical and physical pretreatments were applied on raw SW before AD. The efficiency of these pretreatments on both ammonia removal—recovering ammonia salts to be used as fertilizers in agriculture—and the increase of methane production were assessed in batch tests. Since the pH, temperature, and air flow rate heavily influence AS efficiency and the composition of treated SW, these parameters were set individually or in combination. In more detail, the pH was increased from the natural value of SW to 8 or 10, temperature was increased from the room value to 40 °C, and the air flow rate was increased from zero to 5 Lair LSW−1 min−1. AS was generally more efficient at removing ammonia (up to 97%) from raw (non-treated) SW compared to the other treatments. However, the tested pretreatments were not as efficient as expected in increasing the biogas production, because the methane yields of all pretreated substrates were lower (by about 10–50%) to compared raw SW. The inhibitory effect on AD could have been due to the lack of nutrients and organic matter in the substrate (due to the excessive removal of the pretreatments), the concentration of toxic compounds (such as metal ions or furfural due to water evaporation), and an excess of alkali ions (used to increase the pH in AS). Overall, AS can be considered a sustainable process for the recovery of ammonium sulphate and the removal of other polluting compounds (e.g., organic matter) from SW. Conversely, the use of AS and other chemical and/or thermal processes tested in this study as pretreatments of SW before AD is not advised because these processes appear to reduce methane yields.


2019 ◽  
Vol 41 (19) ◽  
pp. 2510-2517 ◽  
Author(s):  
Junyuan Guo ◽  
XiaoJuan Jia ◽  
Lan Yang ◽  
Guojun Zhang ◽  
Lu Zhang

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