Effectiveness of the “Sterius 60” SHF Radiation Installation for Disinfection of Objects Contaminated with PBA of Groups I–IV, when Working with Infected Biomodels

The aim was to evaluate the effectiveness of using the “Sterius 60” microwave disinfection system (Russia) for decontamination of objects infected with PBA of groups I–IV emerging as a result of working with infected laboratory animals.Materials and methods. Effectiveness verification of disinfection of biological waste generated as a result of the life of laboratory animals by SHF radiation was carried out in the microwave system “Sterius 60”, recommended by the manufacturer for disinfection of epidemiologically hazardous and extremely dangerous medical waste, including biological ones (classes B and C), by volumetric SHF heating. Carcasses of uninfected laboratory animals (white mice, Guinea pigs, suckling rabbits), granulated feed and bedding material (wood shavings), which are objects directly in contact with biomodels, were used as vivarium waste to be decontaminated. The following microorganisms were utilized as model test ones: Bacillus subtilus VKM B-911, Bacillus stearothermophilus VKM B-718, Bacillus licheniformis G VKM B-1711-D, Alcaligenes faecalis 415, Yersinia pestis EV, Bacillus anthracis STI. Laboratory utensils (plastic Petri dishes, porcelain mortars and pestles) were used as a mock-up chamber filler for model test microorganisms.Results and discussion. As a result of the study, data were obtained indicating that the microwave system for disinfection of medical waste “Sterius 60” is ineffective for decontamination of biological waste in laboratories working with biomodels infected with PBA of groups I–II. The established standard mode of disinfection of this system was effective only for non-spore forms of microorganisms, pathogenicity groups III–IV. Therefore, in our opinion, it is advisable to use it for decontamination of laboratory utensils infected with PBA of groups III–IV, directly at sites of waste generation.

117 Effects of feeding a Bacillus Subtilus active microbial on the clinical health, performance and carcass characteristics of feedlot steers

Cross-bred steers (n = 397; initial BW = 247 ± 21 kg) from a single ranch were used to determine the effects of supplementing a Bacillus subtilus direct-fed microbial (DFM; CLOSTAT, Kemin Animal Health, Des Moines, IA) at a rate of 13 g ∙ steer−1 ∙ d−1 on feedlot performance, carcass characteristics, and clinical health during receiving and finishing. Steers were randomly assigned to pens by BW and pens were pairwise assigned 1 of 2 experimental treatments, control (CON) or DFM. Steers were housed in 12.2 x 30.5 m soil surfaced pens, and pen served as the experimental unit. There were no differences during the receiving period for overall BW (P = 0.97), ADG (P = 0.91), DMI (P = 0.77), or G: F (P = 0.79). There was a tendency (P = 0.09) for the DFM fed steers to be 14% more efficient from d 0 to 14 of the receiving period. There were also no differences in final BW (P = 0.40) or overall DMI (P = 0.46) during finishing, but there was a difference in ADG (P = 0.03) from d 29 to 56 of the finishing period where steers supplemented with DFM had greater ADG. The DFM supplemented steers also tended to be more efficient overall during finishing and for the entirety of the experiment (P = 0.07 and P = 0.08, respectively). There were no differences in carcass characteristics (P ≥ 0.31) between the experimental treatments. While limited research has been conducted examining the effects of Bacillus subtilis on feedlot performance, carcass characteristics, and the clinical health of growing and finishing steers, the results of this experiment suggest that the supplementation of a strain Bacillus subtilis at a rate of 13 g ∙ steer−1 ∙ d−1 may improve feed efficiency

XYLANASE PRODUCTION FROM LOCAL BACTERIAL ISOLATE

Seventeen local isolates of Bacillus were isolated from soil to produce extracellular xylanase under submerged fermentation process by using xylan as carbon sole source. All isolates were subjected to quantitative scanning to select the most efficient one. The highest activity of xylanase (2680u/ml) was obtained from isolate Bacillus sp RS1. The isolate identified by 16SrRNA gene sequence of Bacillus subtilis ( accuracy of 99%)which was matched with sequence of Bacillus subtilis VBN25 that recorded in Genebank under the Accession Number of MG027675.1.Extracted xylan from agricultural waste by acidic method(papyrus, sun flower stalks, Ibaa Wheat type, Furat wheat type and Abo Ghraib wheat type)were used as the substrate for xylanase production from Bacillus. The results showed that the papyrus gave the highest amount of xylan (187.6 µg/ml) as compared with that of the sun flower stalks, Ibaa Wheat type, Furat wheat type and Abo Ghraib wheat type(161.3, 161.6, 157.6, 157.2) µ g/ml respectively. The results indicated that the highest xylanase activity was 2800 u/ml produced by Bacillus subtilus when Papyrus xylan was used.

Total Synthesis and Antibacterial Screening of (±)-6,8-Dihydroxy-3- undecyl-3,4-dihydroisochromen-1-one: A Structural Analogue of Metabolites from Ononis natrix

Background: (±)-6,8-Dihydroxy-3-undecyl-3,4-dihydroisochromen-1-one is one of the structural analog of several substituted undecylisocoumarins isolated from Ononis natrix (Fabaceae), has been successfully synthesized by direct condensation of homopthalic acid (1) with undecanoyl chloride yields isochromen-1-one (2). Methods: Alkaline hydrolysis of (2) gave the corresponding keto-acid (3), which is then reduced to hydroxy acid (4) then its cyclodehydration was carried out with acetic anhydride to afford 3,4- dihydroisochromen-1-one (5). Followed by demethylation step, the synthesis of target 6,8- dihydroxy-7-methyl-3-undecyl-3,4-dihydroisocoumarin (6) was achieved. Results: In vitro antibacterial screening of all the synthesized compounds were carried out against ten bacterial strains by agar well diffusion method. Conclusion: Newly synthesized molecules exhibited moderate antibacterial activity and maximum inhibition was observed against Bacillus subtilus and Salmonella paratyphi.

Évaluation de la teneur des composés phénoliques, des proprieties antioxydantes et antimicrobiennes de l’espèce Erica arborea L. (Ericaceae) dans la médecine traditionnelle du Tell sétifien dans l’Est Algérien

Les sommités florales d’Erica arboea L. sont largement utilisées sous forme d’infusion en médecine traditionnelle et sont recommandées dans le Tell sétifien pour traiter et prévenir les infections urinaires aiguës et chroniques principalement. Nous avons soumis les extraits aqueux des feuilles et des fleurs à un dosage des polyphénols, des flavonoïdes et à la détermination des activités antioxydante et antimicrobienne. Les teneurs en composés phénoliques et flavonoïdes totaux ont montré une corrélation avec les activités antioxydantes évaluées. Les tests antioxydants utilisés (DPPH, FRAP, CAT, blanchiment du β-carotène et piégeage du radical hydroxyle) ont montré que le test du DPPH a donné la meilleure activité radicalaire. En outre, l’extrait des feuilles s’est mieux exprimé dans les tests du DPPH, du FRAP et du β- carotène. Les tests de la CAT et le radical hydroxyle ont par contre révélé une activité antioxydante plus importante pour l’extrait de fleurs. Pour l’activité antimicrobienne, on a utilisé la méthode de diffusion en milieu gélosé en ayant recours à la méthode des puits. Les deux extraits ont montré une activité antimicrobienne contre les bactéries à Gram positif Staphylococcus aureus ATCC25923, Bacillus subtilus CLAM20302, Bacillus cereus CLAMH300. L’extrait des feuilles a été plus actif avec une valeur variant de 15 à 23 mm comparé à celui des fleurs (17–18,5 mm). Les valeurs de la concentration minimale inhibitrice ont été trouvées dans une gamme variant de 6,25 à 25 mg/ml pour les extraits des feuilles et des fleurs respectivement. Les résultats de l’étude peuvent enrichir les données existantes et montrent que les feuilles et les fleurs d’Erica arborea L. constituent une source d’agents antioxydants et antibactériens.

Development of technology of disinfectants based on soda production waste

The main requirements for the development of fundamentally new disinfectants with a high level of efficiency are to reduce toxicity by increasing the technical and economic indicators in the technology of their application. In this paper, an innovative composition of a disinfectant based on iodine monochloride in the complex of waste production of soda and polyvinylpyrrolidone is proposed. The dependence of stability and antibacterial activity of the developed disinfectant is studied. High indicators of stability (3 years) and antibacterial activity were obtained on the example of Staphylococcus aureus (S. aureus), Enterococcus fecalis (E. fecalis), Pseudomonas aeruginosa (P. aerginosa), Bacillus subtilus (B. Subtilis No. 12) at the concentration 0.027-0.055, DS versus commercial drug Zhavilar. The antibacterial activity of the disinfectant DS, DS-1 is maintained up to 0.0044-0.0088 for Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and Escherichia coli and exceeds the efficiency of the commercial drug Betadine by 8-28 times. Thus, the developed formulations are highly effective, while low-toxic and safe for the environment and humans.

Investigation on Potential Starter of Bacillus spp. for Ivorian Cocoa Beans Fermentation Improvement

To investigate some interesting functional properties of Bacillus spp. in cocoa fermentation processing, 702 strains of Bacillus sp. strains were isolated from six main Ivorian cocoa producer regions. Three mains technological and useful properties for good cocoa beans fermentation were monitored. Among the 386 pectinolytic Bacillus spp. strains, 86.79% of them were confirmed for enzymes production in liquid medium. These 335 pectinolytic Bacillus strains displayed variety of pectinolytic activity with 50.45% of them producing both pectinase and polygalacturonase (PG) enzymes. Among these 169 strains, 70% displayed both the ability to acidify the medium and to degrade the citrate. In mimic conditions of pH and temperature variations as for fermentation heap, 11 strains were able to maintain their activity ranged within 2.12 ± 0.02 to 0.41 ± 0.03 µmol/min/mg of proteins for PG and 2.36 ± 0.03 to 0.25 ± 0.03 µmol/min/mg of proteins for pectinase. Two species (T10T2 and T6HS10) identified as Bacillus subtilus and Bacillus thuringiensis, were selected as the best based on pectinase production level. They were able to continuously produce both pectinolytic enzymes in different conditions of organic acids and ethanol contents. The highlighted properties in this study and the performance of these two strains may make them best candidate as starters for cocoa fermentation control.

Efecto de los Bacilli como aditivo alimenticio en la respuesta inmune de la camella y de su guelfo recién nacido

La preñez en el dromedario se asocia con la regulación descendente de las respuestas inmunitarias que pueden llevar a la susceptibilidad a infecciones virales y bacterianas por lo que se espera que la alimentación a base de probióticos puede ayudar a fortalecer la inmunidad de las camellas preñadas. Este estudio tuvo como objetivo evaluar el impacto de la mezcla de bacilos en la inmunidad de las camellas preñadas y en el periparto de sus güelfos recién nacidos. Un total de 10 camellas durante su gestación tardía hasta un mes después del parto se dividieron en dos grupos iguales: El G1 (control) se alimentó con la dieta basal, mientras que G2 fue alimentado con dieta suplementada con una mezcla de [Bacillus subtilus - Bacillus licheniformis] (Potenciador 1.3, 10g/cabeza/día). El plasma de las camellas, guelfos y leche se recolectaron en diferentes ensayos. Los resultados para las camellas revelaron un aumento significativo de los niveles de IgG al comparar el G2 con G1 un mes antes del parto, una semana y un mes después del parto. Los niveles de IgG en leche mostraron un aumento significativo en el G2 a la semana después del parto también. Los niveles de IgG de los güelfos mostraron aumentos significativos en G2 en todos los periodos. Las IgA en las camellas y güelfos no mostraron cambios significativos en absoluto. Los niveles de IgA de leche mostraron aumentos en G2 más que en G1 en el momento del parto y una semana después del parto. El nivel del IFN-γ en el plasma de camellas reveló el aumento significativo en el G2 más que en el G1 en el momento del parto y a la semana mientras que en leche, los niveles demostraron el aumento significativo en G2 solamente una semana post-parto y en güelfos, los niveles eran más altos en G2 a la semana y un mes después del nacimiento. En conclusión, la inclusión de un aditivo a base de una mezcla de bacilos en la alimentación de las camellas preñadas mejoró algunos de sus parámetros inmunológicos y de sus güelfos recién nacidos.