Objective:
Colorectal Cancer (CRC) is one of the most common types of cancer in both sexes; it is
considered to be the third leading death factor among other types of cancer.
:
This study aimed to examine the cytotoxicity of a new fluorine boron hybrid complex [L(BF2)2] on human
colorectal adenocarcinoma cell line (HT-29), based on the potency of the half-metal based complexes to initiate
apoptosis.
Methods:
Methods: Based on this data, the impact of it in different concentrations on HT-29 cancerous cells was
determined by apoptosis (ELISA, DNA fragmentation laddering, AO/EB staining), cytotoxicity (MTT) and
genotoxicity (comet assay). We also calculated the cellular Oxidative Stress Index (OSI) by measuring the Total
Antioxidant Status (TAS) and Total Oxidant Status (TOS).
Results:
Firstly, [L(BF2)2] was examined in view of cytotoxic effect in seven various cell lines (HELA, DU-145,
PC3, DLD-1, ECC, PNT1-A and HT-29), and then it was found that the applied complex had a mighty
antiproliferative action on HT-29 cells. Thus, the most effective IC50 value turned out to be 26.49 µM in HT-29
cell line. The present study found a tremendous efficacy of [L(BF2)2] on HT-29 cells, especially in terms of
damage to cancer cells' DNA, and consequently caused a series of reactions leading to programmed cell death.
Conclusion:
The results suggest that the [L(BF2)2] as a novel fluorine boron hybrid complex can induce the
apoptosis of HT-29 colorectal cancerous cell line and is a possible candidate for future cancer studies.
Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin
