Preparation of core-shell PAN nanofibers encapsulated α-tocopherol acetate and ascorbic acid 2-phosphate for photoprotection

In this paper, a novel hIg E aptamer biosensor was designed base on the core-shell Fe3O4@Au magnetic composite nanoparticles (Fe3O4@Au NPs). Firstly, Fe3O4@Au NPs were prepared by one-step reduction process with Fe3O4 nanoparticles (Fe3O4 NPs) as magnetic core and hydroxylamine hydrochloride as deoxidizer. Then, the morphology, composition, and properties of Fe3O4@Au NPs were characterized by scanning electron microscope (SEM), fourier translation infrared spectroscopy (FT-IR), vibrating sample magnetometer (VSM) and so on. Lastly, Human immunoglobulin E (hIg E) was used as the model analyte, a hIg E aptamer biosensor was presented which the hIg E antibody is covalently immobilized as the capture probe on Fe3O4@Au NPs surface, and hIg E aptamer was used as the detection probe. After the hIg E antigen was captured, the ascorbic acid 2-phosphate (AAP) formed ascorbic acid (AA) in the presence of alkaline phosphatase (ALP). The AA reduced the silver ions (Ag+) in the solution to silver metal that preferentially deposited on surface of the Fe3O4@Au NPs. The amount of deposited silver could be quantified using the electrochemical methods. The oxidation current of Ag0 was linear with the concertration of hIg E over the range 0.25~2.0 μg/mL. Therefore, the hIg E aptamer biosensor possessed higher sensitivity, low detection limit and rapid response speed.

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Simultaneous electrochemical determination of dopamine and ascorbic acid using AuNPs@polyaniline core–shell nanocomposites modified electrode

Talanta ◽

10.1016/j.talanta.2011.12.002 ◽

2012 ◽

Vol 89 ◽

pp. 136-141 ◽

Cited By ~ 61

Author(s):

Limin Yang ◽

Shufeng Liu ◽

Qixiu Zhang ◽

Feng Li

Keyword(s):

Ascorbic Acid ◽

Modified Electrode ◽

Electrochemical Determination ◽

Core Shell

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ІНТЕНСИВНІСТЬ ПРОЦЕСІВ ПОЛ У КРОВІ КУРЧАТ–БРОЙЛЕРІВ НА ТЛІ ВАКЦИНАЦІЇ ПРОТИ ХВОРОБИ НЮКАСЛА ТА ЗА ДІЇ ВІТАМІНІВ E ТА C

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.15421/nvlvet7048 ◽

2016 ◽

Vol 18 (3(70)) ◽

pp. 200-204 ◽

Cited By ~ 1

Author(s):

L.V. Romanovich ◽

B.M. Kurtyak ◽

М.S. Romanovich ◽

D.I. Mudrak

Keyword(s):

Lipid Peroxidation ◽

Ascorbic Acid ◽

Research Group ◽

Broiler Chickens ◽

Negative Impact ◽

Stress Factors ◽

Poultry Feed ◽

Control Group ◽

Lipid Hydroperoxides ◽

Tocopherol Acetate

The article presents research data on indices of lipid peroxidation (TBA–active products and hydroperoxides lipids) in the blood of broiler chickens on the background of vaccination and disease Nyukasla for the actions of vitamins E and C.The study was conducted on three groups of 100 broiler birds each. Control group fed normal chicken feed. The first research group in addition to poultry feed said received – tocopherol acetate in an amount of 0.1 g/kg feed, the second – ascorbic acid 0.25 g/kg feed. The third research group chickens – tocopherol acetate and ascorbic acid at these doses. For research use blood that was in the chicken after decapitation at different ages: 11–, 27–, 34– and 41–day age. The results of performance of different age groups broiler chickens compared with the value of the index poultry 11–day age for vaccination, and a control group of birds.Studies have shown that the content of intermediate and final products of lipid peroxidation in the blood plasma of broilers depends on the age and period of immunization. Feeding high amounts of vitamin E and C in the composition of feed for broiler chickens caused a reduction (p < 0.05 – 0.001) content in plasma lipid hydroperoxides and TBA–active products. PAUL likely lower rates of broiler chickens research groups suggest reducing the negative impact of stress factors on their bodies on the background of vaccination against the disease Nyukasla. These changes were more pronounced in the blood of chickens, which in addition to the basic diet receiving tocopherol acetate and ascorbic acid.

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The influence of vitamin E and C feeding on protein content and ratios of its fractions in broiler chickens serum

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.32718/nvlvet9723 ◽

2020 ◽

Vol 22 (97) ◽

pp. 141-146

Author(s):

L. V. Romanovych

Keyword(s):

Ascorbic Acid ◽

Protein Content ◽

Broiler Chickens ◽

Protein Biosynthesis ◽

Control Group ◽

Total Protein Content ◽

Globulin Fraction ◽

Tocopherol Acetate ◽

Vitamins E And C ◽

Combined Use

Protein content and the ratio of its fractions were investigated in the serum of broiler chickens during the growing period with the addition of vitamins E and C to their ration. The investigations were performed in one of the farms in Lviv region on four groups of 100 broiler chickens in each, starting from 1- to 41-day-olds. Chickens in the control group received a standard ration, the first experimental diet with tocopherol acetate, the second experimental – ascorbic acid, and the third – a complex of these vitamins. Poultry research were conducted at different ages: 27-, 34-, and 41-day-olds. The content of common protein was determined in blood serum – biuret method and the range of soluble proteins – by vertical electrophoresis in plates of 7.5 % polyacrylamide gel. Conducted research have shown that the use of tocopherol acetate and ascorbic acid supplements to compound feed of broiler chickens caused a rise in serum total protein content. However, these changes were more pronounced in chickens with the combined use of vitamins E and C in all search periods (P < 0.05–0.01), and also in 11-day-old broilers (P < 0.05), who used ascorbic acid supplement. In the search of the ratio of protein fractions was ascertained an increase in the content of γ-globulin fraction in chickens from the search groups regarding controls at 11 and 27 days of age. Herewith the differences were likely to be in broiler chickens, who used the tocopherol acetate supplement. Instead, 11-day-old chicks in this group had a lower b-globulin fraction (P < 0.05). However, in the serum of chickens from the experimental groups regarding control at 41 days of age a significantly higher content of the α-globulin fraction was registered. In general, the results of the searches showed that additional introduction of tocopherol acetate and ascorbic acid to chickens ration has a stimulating influence on the processes of protein biosynthesis and immune function in the organism of chickens in the process of their growth. This influence was more expressed in chickens with the combined use of vitamins E and C.

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