99 Effects of Combined TLR Stimulation on Interleukin-27 mRNA Expression in Human Monocyte-derived Dendritic Cells

ABSTRACT Neisseria meningitidis serogroup B is a major cause of life-threatening meningitis and septicemia worldwide, and no effective vaccine is available. Initiation of innate and acquired immune responses to N. meningitidis is likely to be dependent on cellular responses of dendritic cells (DC) to antigens present in the outer membrane (OM) of the meningococcus. In this study, the responses of human monocyte-derived DC (mo-DC) to OM isolated from parent (lipopolysaccharide [LPS]-replete) meningococci and from a mutant deficient in LPS were investigated. Parent OM selectively up-regulated Toll-like receptor 4 (TLR4) mRNA expression and induced mo-DC maturation, as reflected by increased production of chemokines, proinflammatory cytokines, and CD83, CD80, CD86, CD40, and major histocompatibility complex (MHC) class II molecules. In contrast, LPS-deficient OM selectively up-regulated TLR2 mRNA expression and induced moderate increases in both cytokine production and expression of CD86 and MHC class II molecules. Preexposure to OM, with or without LPS, augmented the allostimulatory properties of mo-DC, which induced proliferation of naive CD4+ CD45RA+ T cells. In addition, LPS-replete OM induced a greater gamma interferon/interleukin-13 ratio in naive T cells, whereas LPS-deficient OM induced the reverse profile. These data demonstrate that components of the OM, other than LPS, are also likely to be involved in determining the levels of DC activation and the nature of the T-helper immune response.

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The Expressions of TSLP, IL-33, and IL-17A in Monocyte Derived Dendritic Cells from Asthma and COPD Patients are Related to Epithelial–Macrophage Interactions

Cells ◽

10.3390/cells9091944 ◽

2020 ◽

Vol 9 (9) ◽

pp. 1944

Author(s):

Magdalena Paplinska-Goryca ◽

Paulina Misiukiewicz-Stepien ◽

Malgorzata Proboszcz ◽

Patrycja Nejman-Gryz ◽

Katarzyna Gorska ◽

...

Keyword(s):

Dendritic Cells ◽

Dendritic Cell ◽

Mrna Expression ◽

Human Monocyte ◽

Chronic Obstructive ◽

Obstructive Pulmonary Disease ◽

Copd Patients ◽

Culture Model ◽

Triple Cell ◽

Positive Correlations

Background. The cross-talk between the external and internal environment in the respiratory tract involves macrophage/dendritic cell (DC) transepithelial network. Epithelium triggers dendritic cell-mediated inflammation by producing thymic stromal lymphopoietin (TSLP), IL-33, and IL-17A. The study aimed to evaluate the expression of TSLP, IL-33, and IL-17A in human monocyte derived dendritic cells (moDCs) co-cultured with respiratory epithelium and monocyte derived macrophages (moMφs) in asthma, chronic obstructive pulmonary disease (COPD) and healthy controls. Methods. The study used a triple-cell co-culture model, utilizing nasal epithelial cells, along with moMφs and moDCs. Cells were cultured in mono-, di-, and triple-co-cultures for 24 h. Results. Co-culture with epithelium and moMφs significantly increased TSLP in asthma and did not change IL-33 and IL-17A mRNA expression in moDCs. moDCs from asthmatics were characterized by the highest TSLP mRNA expression and the richest population of TSLPR, ST2, and IL17RA expressed cells. A high number of positive correlations between the assessed cytokines and CHI3L1, IL-12p40, IL-1β, IL-6, IL-8, TNF in moDCs was observed in asthma and COPD. Conclusion. TSLP, IL-33, and IL-17A expression in moDCs are differently regulated by epithelium in asthma, COPD, and healthy subjects. These complex cell–cell interactions may impact airway inflammation and be an important factor in the pathobiology of asthma and COPD.

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Chemokine and chemokine receptor mRNA expression is differentially regulated during IL-4 and GM-CSF-dependent differentiation of human monocyte-derived dendritic cells

Journal of Dermatological Science ◽

10.1016/s0923-1811(98)83549-4 ◽

1998 ◽

Vol 16 ◽

pp. S92

Author(s):

Hilke Block ◽

Ulrich Mrowietz

Keyword(s):

Dendritic Cells ◽

Mrna Expression ◽

Chemokine Receptor ◽

Human Monocyte ◽

Gm Csf ◽

Receptor Mrna

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Expression of somatostatin, cortistatin, and somatostatin receptors in human monocytes, macrophages, and dendritic cells

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.00048.2003 ◽

2003 ◽

Vol 285 (2) ◽

pp. E344-E353 ◽

Cited By ~ 98

Author(s):

Virgil A. S. H. Dalm ◽

P. Martin van Hagen ◽

Peter M. van Koetsveld ◽

Sam Achilefu ◽

Adriaan B. Houtsmuller ◽

...

Keyword(s):

Dendritic Cells ◽

Mrna Expression ◽

Immune Cell ◽

Rank Order ◽

Somatostatin Receptors ◽

Human Monocyte ◽

Time Dependent ◽

Mrna Levels ◽

Human Monocytes ◽

Activated State

Increasing evidence suggests that neuropeptides play a role in the regulatory mechanisms between the neuroendocrine and immune systems. A differential expression of the five known somatostatin (SS) receptors (sst1–5) has been demonstrated in human immune cells and tissues. However, little is known concerning regulation and expression of sst1–5and the peptide SS. Therefore, we investigated the expression and the time-dependent regulation of sst1–5, SS, and cortistatin (CST), a novel SS-like peptide, in human monocytes (MO), monocyte-derived macrophages (MP), and dendritic cells (DC) in the basal and lipopolysaccharide (LPS)-activated state. MO, MP, and DC selectively expressed sst2mRNA. SS mRNA was not detectable, whereas all samples expressed CST mRNA. Expression levels of sst2and CST mRNA showed marked differences and were in the rank order of MP>>DC>>>MO. LPS stimulation did not induce expression of SS or sst1,3,4,5. However, sst2mRNA expression was upregulated significantly by stimulation with LPS. CST mRNA was upregulated as well. During differentiation of MO in MP or DC, time-dependent, significantly increasing sst2and CST mRNA levels were found. By confocal microscopy, the presence of sst2receptors was demonstrated on MP, but not on DC. This study demonstrates for the first time a selective and inducible expression of the recently discovered CST, as well as sst2, in human monocyte-derived cells, suggesting a role for a CST-sst2system rather than a SS-sst2system in these immune cell types.

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Interleukin-1β and surface marker expression changes induced by tetrachloroplatinate in human monocyte-derived dendritic cells

Immunopharmacology and Immunotoxicology ◽

10.1080/08923970903095306 ◽

2009 ◽

Vol 00 (00) ◽

pp. 090824065221002-10

Author(s):

Joanna Arkusz ◽

Dobrosława Gradecka-Meesters ◽

Maciej Stępnik

Keyword(s):

Dendritic Cells ◽

Human Monocyte ◽

Surface Marker ◽

Interleukin 1Β ◽

Surface Marker Expression ◽

Marker Expression

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Faculty Opinions recommendation of Multiple signaling pathways contribute to synergistic TLR ligand-dependent cytokine gene expression in human monocyte-derived macrophages and dendritic cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1156938.617001 ◽

2009 ◽

Author(s):

Stephen Schwartz

Keyword(s):

Gene Expression ◽

Dendritic Cells ◽

Signaling Pathways ◽

Human Monocyte ◽

Cytokine Gene Expression ◽

Cytokine Gene ◽

Multiple Signaling

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Human Herpesvirus 6A Induces Dendritic Cell Death and HMGB1 Release without Virus Replication

Pathogens ◽

10.3390/pathogens10010057 ◽

2021 ◽

Vol 10 (1) ◽

pp. 57

Author(s):

Rasmus Gustafsson

Keyword(s):

Cell Death ◽

Dendritic Cells ◽

High Mobility ◽

Human Herpesvirus ◽

Human Monocyte ◽

Hmgb1 Protein ◽

Innate And Adaptive Immunity ◽

Th2 Polarization ◽

Dependent Cell ◽

Ifn Γ

Human herpesvirus 6A (HHV-6A) is a common virus that has important immunomodulatory effects. Dendritic cells (DC) are key players in innate and adaptive immunity and are implicated in the pathogenesis of many human diseases, including infections. (1) Background: Previous studies have demonstrated suppressive effects of HHV-6A on key DC functions. (2) Methods: human monocyte derived dendritic cells were inoculated with HHV-6A and viral replication, cell viability, and release of high mobility group box 1 (HMGB1) protein from DC and of the cytokines IL-2, IL-4, IL-6, IL-10, TNF and IFN-γ after co-culture with allogenic CD4+ T cells were assessed. (3) Results: Nonproductive infection of HHV-6A in DC leads to titer-dependent cell death and the release of HMGB1 protein, and a Th2 polarization. (4) Conclusion: These immune responses aimed to clear the infection may also imply risks for inflammatory pathologies associated with HHV-6A such as multiple sclerosis.

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