Chemokine and chemokine receptor mRNA expression is differentially regulated during IL-4 and GM-CSF-dependent differentiation of human monocyte-derived dendritic cells

1998 ◽  
Vol 16 ◽  
pp. S92
Author(s):  
Hilke Block ◽  
Ulrich Mrowietz
Keyword(s):  
Dendritic Cells ◽  
Mrna Expression ◽  
Chemokine Receptor ◽  
Human Monocyte ◽  
Gm Csf ◽  
Receptor Mrna

scholarly journals Activation of Human Dendritic Cells Is Modulated by Components of the Outer Membranes of Neisseria meningitidis

2003 ◽  
Vol 71 (10) ◽  
pp. 5590-5597 ◽  
Author(s):  
Tamara Al-Bader ◽  
Myron Christodoulides ◽  
John E. Heckels ◽  
Judith Holloway ◽  
Amanda E. Semper ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Neisseria Meningitidis ◽  
Mrna Expression ◽  
Mhc Class Ii ◽  
Human Monocyte ◽  
Class Ii ◽  
Effective Vaccine ◽  
Tlr4 Mrna ◽  
Mhc Class Ii Molecules

ABSTRACT Neisseria meningitidis serogroup B is a major cause of life-threatening meningitis and septicemia worldwide, and no effective vaccine is available. Initiation of innate and acquired immune responses to N. meningitidis is likely to be dependent on cellular responses of dendritic cells (DC) to antigens present in the outer membrane (OM) of the meningococcus. In this study, the responses of human monocyte-derived DC (mo-DC) to OM isolated from parent (lipopolysaccharide [LPS]-replete) meningococci and from a mutant deficient in LPS were investigated. Parent OM selectively up-regulated Toll-like receptor 4 (TLR4) mRNA expression and induced mo-DC maturation, as reflected by increased production of chemokines, proinflammatory cytokines, and CD83, CD80, CD86, CD40, and major histocompatibility complex (MHC) class II molecules. In contrast, LPS-deficient OM selectively up-regulated TLR2 mRNA expression and induced moderate increases in both cytokine production and expression of CD86 and MHC class II molecules. Preexposure to OM, with or without LPS, augmented the allostimulatory properties of mo-DC, which induced proliferation of naive CD4+ CD45RA+ T cells. In addition, LPS-replete OM induced a greater gamma interferon/interleukin-13 ratio in naive T cells, whereas LPS-deficient OM induced the reverse profile. These data demonstrate that components of the OM, other than LPS, are also likely to be involved in determining the levels of DC activation and the nature of the T-helper immune response.

Chemokine and chemokine receptor mRNA expression in human platelets

Cytokine ◽  
1995 ◽  
Vol 7 (6) ◽  
pp. 479-482 ◽  
Author(s):  
Christine A. Power ◽  
Jeannine M. Clemetson ◽  
Kenneth J. Clemetson ◽  
Timothy N.C. Wells
Keyword(s):  
Mrna Expression ◽  
Chemokine Receptor ◽  
Human Platelets ◽  
Receptor Mrna

99 Effects of Combined TLR Stimulation on Interleukin-27 mRNA Expression in Human Monocyte-derived Dendritic Cells

Cytokine ◽  
2007 ◽  
Vol 39 (1) ◽  
pp. 27
Author(s):  
Claudius U. Meyer ◽  
Doreen Krumbiegel ◽  
Helena Markus ◽  
Michaela Fuidl ◽  
Fred Zepp
Keyword(s):  
Dendritic Cells ◽  
Mrna Expression ◽  
Human Monocyte ◽  
Interleukin 27

Differential Effects of Immunosuppressive Drugs on Chemokine Receptor CCR7 in Human Monocyte-Derived Dendritic Cells: Selective Upregulation by Rapamycin

2006 ◽  
Vol 82 (6) ◽  
pp. 826-834 ◽  
Author(s):  
Valeria Sordi ◽  
Giancarlo Bianchi ◽  
Chiara Buracchi ◽  
Alessia Mercalli ◽  
Federica Marchesi ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Chemokine Receptor ◽  
Human Monocyte ◽  
Immunosuppressive Drugs ◽  
Differential Effects

Serial Analysis of Gene Expression in Human Monocyte-Derived Dendritic Cells

Blood ◽  
1999 ◽  
Vol 94 (3) ◽  
pp. 845-852 ◽  
Author(s):  
Shin-ichi Hashimoto ◽  
Takuji Suzuki ◽  
Hong-Yan Dong ◽  
Shigenori Nagai ◽  
Nobuyuki Yamazaki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dendritic Cells ◽  
Cell Structure ◽  
Human Monocyte ◽  
Interleukin 4 ◽  
Maturation Stage ◽  
Blood Monocytes ◽  
Gm Csf ◽  
Highly Expressed Genes

Dendritic cells (DCs) are professional antigen-presenting cells in the immune system and can be generated in vitro from hematopoietic progenitor cells in the bone marrow, CD34+ cord blood cells, precursor cells in the peripheral blood, and blood monocytes by culturing with granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-4, and tumor necrosis factor-. We have performed serial analysis of gene expression (SAGE) in DCs derived from human blood monocytes. A total of 58,540 tag sequences from a DC complementary DNA (cDNA) library represented more than 17,000 different genes, and these data were compared with SAGE analysis of tags from monocytes (Mo) and GM-CSF–induced macrophages (M◊). Many of the genes that were differentially expressed in DCs were identified as genes encoding proteins related to cell structure and cell motility. Interestingly, the highly expressed genes in DCs encode chemokines such as TARC, MDC, and MCP-4, which preferentially chemoattract Th2-type lymphocytes. Although DCs have been considered to be very heterogeneous, the identification of specific genes expressed in human Mo-derived DCs should provide candidate genes to define subsets of, the function of, and the maturation stage of DCs and possibly also to diagnose diseases in which DCs play a significant role, such as autoimmune diseases and neoplasms. This study represents the first extensive gene expression analysis in any type of DCs.

scholarly journals The Expressions of TSLP, IL-33, and IL-17A in Monocyte Derived Dendritic Cells from Asthma and COPD Patients are Related to Epithelial–Macrophage Interactions

Cells ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1944
Author(s):  
Magdalena Paplinska-Goryca ◽  
Paulina Misiukiewicz-Stepien ◽  
Malgorzata Proboszcz ◽  
Patrycja Nejman-Gryz ◽  
Katarzyna Gorska ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Dendritic Cell ◽  
Mrna Expression ◽  
Human Monocyte ◽  
Chronic Obstructive ◽  
Obstructive Pulmonary Disease ◽  
Copd Patients ◽  
Culture Model ◽  
Triple Cell ◽  
Positive Correlations

Background. The cross-talk between the external and internal environment in the respiratory tract involves macrophage/dendritic cell (DC) transepithelial network. Epithelium triggers dendritic cell-mediated inflammation by producing thymic stromal lymphopoietin (TSLP), IL-33, and IL-17A. The study aimed to evaluate the expression of TSLP, IL-33, and IL-17A in human monocyte derived dendritic cells (moDCs) co-cultured with respiratory epithelium and monocyte derived macrophages (moMφs) in asthma, chronic obstructive pulmonary disease (COPD) and healthy controls. Methods. The study used a triple-cell co-culture model, utilizing nasal epithelial cells, along with moMφs and moDCs. Cells were cultured in mono-, di-, and triple-co-cultures for 24 h. Results. Co-culture with epithelium and moMφs significantly increased TSLP in asthma and did not change IL-33 and IL-17A mRNA expression in moDCs. moDCs from asthmatics were characterized by the highest TSLP mRNA expression and the richest population of TSLPR, ST2, and IL17RA expressed cells. A high number of positive correlations between the assessed cytokines and CHI3L1, IL-12p40, IL-1β, IL-6, IL-8, TNF in moDCs was observed in asthma and COPD. Conclusion. TSLP, IL-33, and IL-17A expression in moDCs are differently regulated by epithelium in asthma, COPD, and healthy subjects. These complex cell–cell interactions may impact airway inflammation and be an important factor in the pathobiology of asthma and COPD.

scholarly journals Selective Recruitment of Immature and Mature Dendritic Cells by Distinct Chemokines Expressed in Different Anatomic Sites

1998 ◽  
Vol 188 (2) ◽  
pp. 373-386 ◽  
Author(s):  
Marie-Caroline Dieu ◽  
Béatrice Vanbervliet ◽  
Alain Vicari ◽  
Jean-Michel Bridon ◽  
Elisabeth Oldham ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
Mrna Expression ◽  
Interleukin 4 ◽  
Traffic Pattern ◽  
Gm Csf ◽  
Inflammatory Protein ◽  
Tnf Α ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony

DCs (dendritic cells) function as sentinels of the immune system. They traffic from the blood to the tissues where, while immature, they capture antigens. They then leave the tissues and move to the draining lymphoid organs where, converted into mature DC, they prime naive T cells. This suggestive link between DC traffic pattern and functions led us to investigate the chemokine responsiveness of DCs during their development and maturation. DCs were differentiated either from CD34+ hematopoietic progenitor cells (HPCs) cultured with granulocyte/macrophage colony–stimulating factor (GM-CSF) plus tumor necrosis factor (TNF)-α or from monocytes cultured with GM-CSF plus interleukin 4. Immature DCs derived from CD34+ HPCs migrate most vigorously in response to macrophage inflammatory protein (MIP)-3α, but also to MIP-1α and RANTES (regulated on activation, normal T cell expressed and secreted). Upon maturation, induced by either TNF-α, lipopolysaccharide, or CD40L, DCs lose their response to these three chemokines when they acquire a sustained responsiveness to a single other chemokine, MIP-3β. CC chemokine receptor (CCR)6 and CCR7 are the only known receptors for MIP-3α and MIP-3β, respectively. The observation that CCR6 mRNA expression decreases progressively as DCs mature, whereas CCR7 mRNA expression is sharply upregulated, provides a likely explanation for the changes in chemokine responsiveness. Similarly, MIP-3β responsiveness and CCR7 expression are induced upon maturation of monocyte- derived DCs. Furthermore, the chemotactic response to MIP-3β is also acquired by CD11c+ DCs isolated from blood after spontaneous maturation. Finally, detection by in situ hybridization of MIP-3α mRNA only within inflamed epithelial crypts of tonsils, and of MIP-3β mRNA specifically in T cell–rich areas, suggests a role for MIP-3α/CCR6 in recruitment of immature DCs at site of injury and for MIP-3β/CCR7 in accumulation of antigen-loaded mature DCs in T cell–rich areas.

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