Astragaloside IV ameliorates renal injury in streptozotocin-induced diabetic rats through inhibiting NF-κB-mediated inflammatory genes expression

Advanced glycation end-products (AGEs) induce the production of reactive oxygen species (ROS) and extra cellular matrix (ECM) degradation via suppression of neuropilin-1 (NRP-1) and interaction with AGE-receptors (RAGE). This study aimed to reveal whether modulation of NRP-1 by rosuvastatin (RT) prevents AGE-induced renal injury via targeting RAGE/matrix metalloproteinase-2 (MMP-2) signaling in diabetic rats. Treatment with RT ameliorated the altered level of markers of glycemic control, renal injury, cholesterol, triglyceride (TG) and hepatic HMG-CoA reductase activity; the level of circulatory carboxymethyl-lysine (CML) and the accumulation of fluorogenic-AGEs in renal tissue was reduced; the expression of renal NRP-1, a checkpoint target, was stimulated; the transcription of RAGE, NF?B-2, TGF-?1 and MMP-2 was suppressed; the circulatory carbonyl content (CC) and paraoxonase-1 (PON-1) activity was ameliorated, and renal histopathological features were attenuated as evidenced by improved glomerular appearance, Bowman?s space and abundant podocytes in kidneys. In conclusion, RT exhibited the potential to counteract diabetes and AGE-induced renal pathologies via stimulation of NRP-1, suppression of RAGE, and of genes responsible for ECM disintegration (MMP-2) and the inflammatory response (NF?B-2).

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Phenethyl isothiocyanate alleviates renal injury in STZ‐induced diabetic rats: Effect on oxidative stress, glycative stress and inflammation

The FASEB Journal ◽

10.1096/fasebj.2021.35.s1.00390 ◽

2021 ◽

Vol 35 (S1) ◽

Author(s):

Mohamed El‐Sherbiny ◽

Nada Eisa ◽

Eman Said ◽

Nehal Elsherbiny

Keyword(s):

Oxidative Stress ◽

Renal Injury ◽

Diabetic Rats ◽

Phenethyl Isothiocyanate

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Diabetes mellitus increased integrins gene expression in rat endometrium at the time of embryo implantation

International Journal of Reproductive BioMedicine ◽

10.18502/ijrm.v17i6.4810 ◽

2019 ◽

Author(s):

Abbas Bakhteyari ◽

Yasaman Zarrin ◽

Parvaneh Nikpour ◽

Zeinab Sadat Hosseiny ◽

...

Keyword(s):

Gene Expression ◽

Diabetes Mellitus ◽

Group Treatment ◽

Embryo Implantation ◽

Treated Group ◽

Diabetic Rats ◽

Diabetic Rat ◽

Control Group ◽

Genes Expression ◽

Normal Menstrual Cycle

Background: Diabetes mellitus deeply changes the genes expression of integrin (Itg) subunits in several cells and tissues such as monocytes, arterial endothelium, kidney glomerular cells, retina. Furthermore, hyperglycemia could impress and reduce the rate of successful assisted as well as non-assisted pregnancy. Endometrium undergoes thorough changes in normal menstrual cycle and the question is: What happens in the endometrium under diabetic condition? Objective: The aim of the current study was to investigate the endometrial gene expression of α3, α4, αv, Itg β1 and β3 subunits in diabetic rat models at the time of embryo implantation. Materials and Methods: Twenty-eight rats were randomly divided into 4 groups: control group, diabetic group, pioglitazone-treated group, and metformin-treated group. Real-time PCR was performed to determine changes in the expression of Itg α3, α4, αv, β1, and β3 genes in rat’s endometrium. Results: The expression of all Itg subunits increased significantly in diabetic rats’ endometrium compared with control group. Treatment with pioglitazone significantly reduced the level of Itg subunits gene expression compared with diabetic rats. While metformin had a different effect on α3 and α4 and elevated these two subunits gene expression. Conclusion: Diabetes mellitus significantly increased the expression of studied Itg subunits, therefore untreated diabetes could be potentially assumed as one of the preliminary elements in embryo implantation failure.

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BOLD MRI to evaluate early development of renal injury in a rat model of diabetes

Journal of International Medical Research ◽

10.1177/0300060517743826 ◽

2018 ◽

Vol 46 (4) ◽

pp. 1391-1403 ◽

Cited By ~ 3

Author(s):

Qidong Wang ◽

Chuangen Guo ◽

Lan Zhang ◽

Rui Zhang ◽

Zhaoming Wang ◽

...

Keyword(s):

Renal Injury ◽

Diabetic Rats ◽

Blood Oxygenation ◽

Dynamic Changes ◽

Bold Mri ◽

Oxygenation Level ◽

Mri Scans ◽

Streptozotocin Induced Diabetes ◽

Magnetic Resonance Imaging Mri ◽

Diabetes Induction

Objective To investigate changes in renal oxygenation levels by blood-oxygenation-level dependent (BOLD)-magnetic resonance imaging (MRI), and to evaluate BOLD-MRI for detecting early diabetic renal injury. Methods Seventy-five rats, with unilateral nephrectomy, were randomly divided into streptozotocin-induced diabetes mellitus (DM, n = 65) and normal control (NC, n = 10) groups. BOLD-MRI scans were performed at baseline (both groups) and at 3, 7, 14, 21, 28, 35, 42, 49, 56, 63 and 70 days (DM only). Renal cortical (C) and medullary (M) R2* signals were measured and R2* medulla/cortex ratio (MCR) was calculated. Results DM-group CR2* and MR2* values were significantly higher than NC values following diabetes induction. R2* values increased gradually and peaked at day 35 (CR2*, 33.95 ± 0.34 s–1; MR2*, 43.79 ± 1.46 s–1), then dropped gradually (CR2*, 33.17 ± 0.69 s–1; MR2*, 41.61 ± 0.95 s–1 at day 70). DM-group MCR rose gradually from 1.12 to 1.32 at day 42, then decreased to 1.25 by day 70. Conclusions BOLD-MRI can be used to non-invasively evaluate renal hypoxia and early diabetic renal injury in diabetic rats. MCR may be adopted to reflect dynamic changes in renal hypoxia.

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