scholarly journals Cellulose-Microtubule Uncoupling Proteins Prevent Lateral Displacement of Microtubules during Cellulose Synthesis in Arabidopsis

2016 ◽  
Vol 38 (3) ◽  
pp. 305-315 ◽  
Author(s):  
Zengyu Liu ◽  
Rene Schneider ◽  
Christopher Kesten ◽  
Yi Zhang ◽  
Marc Somssich ◽  
...  
Keyword(s):  
Lateral Displacement ◽  
Uncoupling Proteins ◽  
Cellulose Synthesis

Single molecule optical diffraction: A tool for understanding the structure of triple stranded left-hand helical cellulose

1989 ◽  
Vol 47 ◽  
pp. 1024-1025
Author(s):  
George C. Ruben ◽  
William Krakow
Keyword(s):  
Single Molecule ◽  
Helical Structure ◽  
Optical Diffraction ◽  
Maximum Diameter ◽  
Primary Cell Wall ◽  
Cellulose Synthesis ◽  
Left Hand ◽  
Left Handed ◽  
Freeze Dried ◽  
Diffraction Patterns

Tobacco primary cell wall and normal bacterial Acetobacter xylinum cellulose formation produced a 36.8±3Å triple-stranded left-hand helical microfibril in freeze-dried Pt-C replicas and in negatively stained preparations for TEM. As three submicrofibril strands exit the wall of Axylinum , they twist together to form a left-hand helical microfibril. This process is driven by the left-hand helical structure of the submicrofibril and by cellulose synthesis. That is, as the submicrofibril is elongating at the wall, it is also being left-hand twisted and twisted together with two other submicrofibrils. The submicrofibril appears to have the dimensions of a nine (l-4)-ß-D-glucan parallel chain crystalline unit whose long, 23Å, and short, 19Å, diagonals form major and minor left-handed axial surface ridges every 36Å.The computer generated optical diffraction of this model and its corresponding image have been compared. The submicrofibril model was used to construct a microfibril model. This model and corresponding microfibril images have also been optically diffracted and comparedIn this paper we compare two less complex microfibril models. The first model (Fig. 1a) is constructed with cylindrical submicrofibrils. The second model (Fig. 2a) is also constructed with three submicrofibrils but with a single 23 Å diagonal, projecting from a rounded cross section and left-hand helically twisted, with a 36Å repeat, similar to the original model (45°±10° crossover angle). The submicrofibrils cross the microfibril axis at roughly a 45°±10° angle, the same crossover angle observed in microflbril TEM images. These models were constructed so that the maximum diameter of the submicrofibrils was 23Å and the overall microfibril diameters were similar to Pt-C coated image diameters of ∼50Å and not the actual diameter of 36.5Å. The methods for computing optical diffraction patterns have been published before.

Structural aspects of tracheary element differentiation in suspension cultures of Zinnia elegans

1989 ◽  
Vol 47 ◽  
pp. 768-769
Author(s):  
C. H. Haigler ◽  
A. W. Roberts
Keyword(s):  
Tracheary Element ◽  
Vesicle Fusion ◽  
Zinnia Elegans ◽  
Cellulose Synthesis ◽  
Tracheary Elements ◽  
Animal Cells ◽  
Mesophyll Cells ◽  
Fixation Techniques ◽  
Localized Patterns ◽  
Structural Aspects

Tracheary elements, the water-conducting cells in plants, are characterized by their reinforced walls that became thickened in localized patterns during differentiation (Fig. 1). The synthesis of this localized wall involves abundant secretion of Golgi vesicles that export preformed matrix polysaccharides and putative proteins involved in cellulose synthesis. Since the cells are not growing, some kind of endocytotic process must also occur. Many researchers have commented on where exocytosis occurs in relation to the thickenings (for example, see), but they based their interpretations on chemical fixation techniques that are not likely to provide reliable information about rapid processes such as vesicle fusion. We have used rapid freezing to more accurately assess patterns of vesicle fusion in tracheary elements. We have also determined the localization of calcium, which is known to regulate vesicle fusion in plant and animal cells.Mesophyll cells were obtained from immature first leaves of Zinnia elegans var. Envy (Park Seed Co., Greenwood, S.C.) and cultured as described previously with the following exceptions: (a) concentration of benzylaminopurine in the culture medium was reduced to 0.2 mg/l and myoinositol was eliminated; and (b) 1.75ml cultures were incubated in 22 x 90mm shell vials with 112rpm rotary shaking. Cells that were actively involved in differentiation were harvested and frozen in solidifying Freon as described previously. Fractures occurred preferentially at the cell/planchet interface, which allowed us to find some excellently-preserved cells in the replicas. Other differentiating cells were incubated for 20-30 min in 10(μM CTC (Sigma), an antibiotic that fluoresces in the presence of membrane-sequestered calcium. They were observed in an Olympus BH-2 microscope equipped for epi-fluorescence (violet filter package and additional Zeiss KP560 barrier filter to block chlorophyll autofluorescence).

scholarly journals Comparison of Novel Seismic Protection Devices to Attenuate the Earthquake Induced Energy

Actuators ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 73
Author(s):  
Osman Hansu ◽  
Esra Mete Güneyisi
Keyword(s):  
Lateral Displacement ◽  
Time History ◽  
Seismic Protection ◽  
Base Shear ◽  
Seismic Demands ◽  
Nonlinear Time History Analyses ◽  
History Of ◽  
Protection Devices ◽  
Nonlinear Time History ◽  
Better Than

This study addresses an alternative use of viscous dampers (VDs) associated with buckling restrained braces (BRBs) as innovative seismic protection devices. For this purpose, 4-, 8- and 12-story steel bare frames were designed with 6.5 m equal span length and 4 m story height. Thereafter, they were seismically improved by mounting the VDs and BRBs in three patterns, namely outer bays, inner bays, and all bays over the frame heights. The structures were modeled using SAP 2000 software and evaluated by the nonlinear time history analyses subjected to the six natural ground motions. The seismic responses of the structures were investigated for the lateral displacement, interstory drift, absolute acceleration, maximum base shear, and time history of roof displacement. The results clearly indicated that the VDs and BRBs reduced seismic demands significantly compared to the bare frame. Moreover, the all-bay pattern performed better than the others.

scholarly journals Three-Axis Pneumatic Haptic Display for the Mechanical and Thermal Stimulation of a Human Finger Pad

Actuators ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 60
Author(s):  
Eun-Hyuk Lee ◽  
Sang-Hoon Kim ◽  
Kwang-Seok Yun
Keyword(s):  
Pulse Width Modulation ◽  
Haptic Feedback ◽  
Control Function ◽  
Lateral Displacement ◽  
Gain Control ◽  
Haptic Display ◽  
Positional Accuracy ◽  
Control Module ◽  
Haptic Displays ◽  
Human Finger

Haptic displays have been developed to provide operators with rich tactile information using simple structures. In this study, a three-axis tactile actuator capable of thermal display was developed to deliver tactile senses more realistically and intuitively. The proposed haptic display uses pneumatic pressure to provide shear and normal tactile pressure through an inflation of the balloons inherent in the device. The device provides a lateral displacement of ±1.5 mm for shear haptic feedback and a vertical inflation of the balloon of up to 3.7 mm for normal haptic feedback. It is designed to deliver thermal feedback to the operator through the attachment of a heater to the finger stage of the device, in addition to mechanical haptic feedback. A custom-designed control module is employed to generate appropriate haptic feedback by computing signals from sensors or control computers. This control module has a manual gain control function to compensate for the force exerted on the device by the user’s fingers. Experimental results showed that it could improve the positional accuracy and linearity of the device and minimize hysteresis phenomena. The temperature of the device could be controlled by a pulse-width modulation signal from room temperature to 90 °C. Psychophysical experiments show that cognitive accuracy is affected by gain, and temperature is not significantly affected.

Lateral obstacle avoidance control based on driving behavior recognition of the preceding vehicles in adjacent lanes

2020 ◽  
Vol 68 (10) ◽  
pp. 880-892
Author(s):  
Youguo He ◽  
Xing Gong ◽  
Chaochun Yuan ◽  
Jie Shen ◽  
Yingkui Du
Keyword(s):  
Obstacle Avoidance ◽  
Lateral Displacement ◽  
Longitudinal Velocity ◽  
Driving Behavior ◽  
Lateral Acceleration ◽  
Lateral Motion ◽  
Behavior Recognition ◽  
Dynamic Surface ◽  
Lane Changing ◽  
The Impact

AbstractThis paper proposes a lateral lane change obstacle avoidance constraint control simulation algorithm based on the driving behavior recognition of the preceding vehicles in adjacent lanes. Firstly, the driving behavior of the preceding vehicles is recognized based on the Hidden Markov Model, this research uses longitudinal velocity, lateral displacement and lateral velocity as the optimal observation signals to recognize the driving behaviors including lane-keeping, left-lane-changing or right-lane-changing; Secondly, through the simulation of the dangerous cutting-in behavior of the preceding vehicles in adjacent lanes, this paper calculates the ideal front wheel steering angle according to the designed lateral acceleration in the process of obstacle avoidance, designs the vehicle lateral motion controller by combining the backstepping and Dynamic Surface Control, and the safety boundary of the lateral motion is constrained based on the Barrier Lyapunov Function; Finally, simulation model is built, and the simulation results show that the designed controller has good performance. This active safety technology effectively reduces the impact on the autonomous vehicle safety when the preceding vehicle suddenly cuts into the lane.

scholarly journals Driven Pile Effects on Nearby Cylindrical and Semi-Tapered Pile in Sandy Clay

2021 ◽  
Vol 11 (7) ◽  
pp. 2919
Author(s):  
Massamba Fall ◽  
Zhengguo Gao ◽  
Becaye Cissokho Ndiaye
Keyword(s):  
Coupling Effect ◽  
Lateral Displacement ◽  
Bending Moment ◽  
Adaptive Mesh ◽  
Pile Driving ◽  
Arbitrary Lagrangian Eulerian ◽  
Axial Forces ◽  
Tapered Pile ◽  
Driven Pile ◽  
The Impact

A pile foundation is commonly adopted for transferring superstructure loads into the ground in weaker soil. They diminish the settlement of the infrastructure and augment the soil-bearing capacity. This paper emphases the pile-driving effect on an existing adjacent cylindrical and semi-tapered pile. Driving a three-dimensional pile into the ground is fruitfully accomplished by combining the arbitrary Lagrangian–Eulerian (ALE) adaptive mesh and element deletion methods without adopting any assumptions that would simplify the simulation. Axial forces, bending moment, and lateral displacement were studied in the neighboring already-installed pile. An investigation was made into some factors affecting the forces and bending moment, such as pile spacing and the shape of the already-installed pile (cylindrical, tapered, or semi-tapered). An important response was observed in the impact of the driven pile on the nearby existing one, the bending moment and axial forces were not negligible, and when the pile was loaded, it was recommended to consider the coupling effect. Moreover, the adjacent semi-tapered pile was subjected to less axial and lateral movement than the cylindrical one with the same length and volume for taper angles smaller than 1.0°, and vice versa for taper angles greater than 1.4°.

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