Methicillin-resistant Staphylococcus aureus carrying SCCmec type II was more frequent than the Brazilian endemic clone as a cause of nosocomial bacteremia

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of infections acquired in both community and hospital settings. In this study, MRSA isolated from different sources of hospitalized patients was characterized by molecular and phenotypic methods. Methodology: A total of 123 S. aureus isolates were characterized according to their genetic relatedness by repetitive element sequence based-PCR (REP-PCR), in vitro antimicrobial susceptibility profile, SCCmec typing and presence of seven virulence factor-encoding genes. Results: REP-PCR fingerprinting showed low relatedness between the isolates, and the predominance of one specific lineage or clonal group was not observed. All isolates were susceptible to teicoplanin and linezolide. All isolates were resistant to cefoxitin and penicillin, and the majority were also resistant to one or more other antimicrobials. Fifty isolates (41.7%) were intermediately resistant to vancomycin. Most isolates harbored SCCmec type II (53.7%), followed by type I (22.8%), type IV (8.1%) and type III (1.6%). All isolates harbored at least two virulence factor-encoding genes, and the prevalence was as follows: coa, 100%; icaA, 100%; hla, 13.0%; hlb, 91.1%, hld, 91.1%; lukS-PV and lukF-PV, 2.4%; and tst, 34.1%. A positive association with the presence of hla and SCCmec type II, and tst and SCCmec type I was observed. Conclusion: This study showed the high virulence potential of multidrug-resistant MRSA circulating in a teaching hospital. A high prevalence of MRSA showing intermediate vancomycin resistance was also observed, indicating the urgent need to improve strategies for controlling the use of antimicrobials for appropriate management of S. aureus infections.

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Comparison of the Staphylococcal Chromosome Cassette (SCC) mec in Methicillin-Resistant Staphylococcus aureus (MRSA) and Non-aureus Staphylococci (MRNAS) from Animals and Humans

Antibiotics ◽

10.3390/antibiotics10030256 ◽

2021 ◽

Vol 10 (3) ◽

pp. 256

Author(s):

Cyrille Ngassam Tchamba ◽

Jean-Noël Duprez ◽

Pierrick Lucas ◽

Yannick Blanchard ◽

Filip Boyen ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Animal Species ◽

Methicillin Resistant Staphylococcus Aureus ◽

Health Hazard ◽

Sccmec Type ◽

Meca Gene ◽

Type Ii ◽

Methicillin Resistant ◽

High Prevalence ◽

Sccmec Types

Methicillin-resistant Staphylococcus aureus (MRSA) and non-aureus staphylococci (MRNAS) cause different infections in animals, including mastitis, in livestock and humans. This study aimed to identify and compare the staphylococcal chromosome cassette mec (SCCmec) types of MRSA or MRNAS isolated from several animal species and humans in different countries. Of 1462 S. aureus and non-aureus staphylococci, 68 grew on Chrom MRSA ID® agar, were phenotypically resistant to cefoxitin and tested positive with the PCR for the mecA gene. These 60 MRSA and 8 MRNAS were isolated in Belgium mainly from cows (livestock-associated (LA) MRS) and humans (community-acquired (CA) MRS) and in Japan from dogs and cats. The SCCmec cassettes were identified by multiplex PCR in 52 MRSA and 7 MRNAS and by whole genome sequencing (WGS) in 8 additional MRSA. The SCCmec types IV and V were the most frequent in Belgian LA-MRS and CA-MRS, while the SCCmec type II was identified in four of the five Japanese MRSA. The remaining isolate was a bovine S. haemolyticus in which no SCCmec was identified. These results confirm the high prevalence of the SCCmec types IV and V in LA-MRS and CA-MRS in Belgium, emphasizing the possible public health hazard of the former, and the absence of SCCmec in some MRNAS.

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Sccmec type II gene is common among clinical isolates of methicillin-resistant Staphylococcus aureus in Jakarta, Indonesia

BMC Research Notes ◽

10.1186/1756-0500-6-110 ◽

2013 ◽

Vol 6 (1) ◽

pp. 110 ◽

Cited By ~ 6

Author(s):

Latre Buntaran ◽

Mochammad Hatta ◽

Andi R Sultan ◽

Ressy Dwiyanti ◽

Muhammad Sabir

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Sccmec Type ◽

Clinical Isolates ◽

Type Ii ◽

Methicillin Resistant

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Methicillin-resistant Staphylococcus aureus in Rio de Janeiro hospitals: Dissemination of the USA400/ST1 and USA800/ST5 SCCmec type IV and USA100/ST5 SCCmec type II lineages in a public institution and polyclonal presence in a private one

American Journal of Infection Control ◽

10.1016/j.ajic.2012.08.008 ◽

2013 ◽

Vol 41 (3) ◽

pp. e21-e26 ◽

Cited By ~ 33

Author(s):

Roberta Mello Ferreira Caboclo ◽

Fernanda Sampaio Cavalcante ◽

Natalia Lopes Pontes Iorio ◽

Ricardo Pinto Schuenck ◽

André Nogueira Olendzki ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Rio De Janeiro ◽

Methicillin Resistant Staphylococcus Aureus ◽

Sccmec Type ◽

Public Institution ◽

Type Ii ◽

Methicillin Resistant ◽

Type Iv

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Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm

Toxins ◽

10.3390/toxins13010014 ◽

2020 ◽

Vol 13 (1) ◽

pp. 14

Author(s):

Thamiris Santana Machado ◽

Felipe Ramos Pinheiro ◽

Lialyz Soares Pereira Andre ◽

Renata Freire Alves Pereira ◽

Reginaldo Fernandes Correa ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Sccmec Type ◽

Protein A ◽

Invasive Infection ◽

Type I ◽

Methicillin Resistant ◽

Type Iv ◽

Spa Gene ◽

The City

Hospitalizations related to Methicillin-resistant Staphylococcus aureus (MRSA) are frequent, increasing mortality and health costs. In this way, this study aimed to compare the genotypic and phenotypic characteristics of MRSA isolates that colonize and infect patients seen at two hospitals in the city of Niterói—Rio de Janeiro, Brazil. A total of 147 samples collected between March 2013 and December 2015 were phenotyped and genotyped to identify the protein A (SPA) gene, the mec staphylococcal chromosomal cassette (SCCmec), mecA, Panton-Valentine Leucocidin (PVL), icaC, icaR, ACME, and hla virulence genes. The strength of biofilm formation has also been exploited. The prevalence of SCCmec type IV (77.1%) was observed in the colonization group; however, in the invasive infection group, SCCmec type II was prevalent (62.9%). The Multilocus Sequence Typing (MLST), ST5/ST30, and ST5/ST239 analyses were the most frequent clones in colonization, and invasive infection isolates, respectively. Among the isolates selected to assess the ability to form a biofilm, 51.06% were classified as strong biofilm builders. Surprisingly, we observed that isolates other than the Brazilian Epidemic Clone (BEC) have appeared in Brazilian hospitals. The virulence profile has changed among these isolates since the ACME type I and II genes were also identified in this collection.

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The First Report of a Methicillin-Resistant Staphylococcus aureus Isolate Harboring Type IV SCCmec in Thailand

Pathogens ◽

10.3390/pathogens10040430 ◽

2021 ◽

Vol 10 (4) ◽

pp. 430

Author(s):

Wichai Santimaleeworagun ◽

Praewdow Preechachuawong ◽

Wandee Samret ◽

Tossawan Jitwasinkul

Keyword(s):

Staphylococcus Aureus ◽

Resistance Genes ◽

Methicillin Resistant Staphylococcus Aureus ◽

Methicillin Resistance ◽

Sccmec Type ◽

Clinical Strain ◽

Spa Typing ◽

Methicillin Resistant ◽

Type Iv ◽

Antimicrobial Resistance Genes

Methicillin-resistant Staphylococcus aureus (MRSA) is mostly found in Thailand in the hospital as a nosocomial pathogen. This study aimed to report the genetic characterization of a clinical community-acquired MRSA (CA-MRSA) isolate collected from hospitalized patients in Thailand. Among 26 MRSA isolates, S. aureus no. S17 preliminarily displayed the presence of a staphylococcal cassette chromosome mec (SCCmec) type IV pattern. The bacterial genomic DNA was subjected to whole-genome sequencing. Panton–Valentine leukocidin (PVL) production, virulence toxins, and antibiotic resistance genes were identified, and multi-locus sequence typing (MLST) and spa typing were performed. The strain was matched by sequence to MLST type 2885 and spa type t13880. This strain carried type IV SCCmec with no PVL production. Five acquired antimicrobial resistance genes, namely blaZ, mecA, Inu(A), tet(K), and dfrG conferring resistance to β-lactams, lincosamides, tetracycline, and trimethoprim, were identified. The detected toxins were exfoliative toxin A, gamma-hemolysin, leukocidin D, and leukocidin E. Moreover, there were differences in seven regions in CR-MRSA no. S17 compared to CA-MRSA type 300. In summary, we have reported the ST2885-SCCmec IV CA-MRSA clinical strain in Thailand for the first time, highlighting the problem of methicillin resistance in community settings and the consideration in choosing appropriate antibiotic therapy.

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Distribution of Virulence Genes Among Methicillin-resistant Staphylococcus Aureus of Clinical and Environmental Origin

10.21203/rs.3.rs-87157/v1 ◽

2020 ◽

Author(s):

Deepshikha Bhowmik ◽

Shiela Chetri ◽

Bhaskar Jyoti Das ◽

Debadatta Dhar Chanda ◽

Amitabha Bhattacharjee

Keyword(s):

Staphylococcus Aureus ◽

Multidrug Resistance ◽

Virulence Genes ◽

Methicillin Resistant Staphylococcus Aureus ◽

Virulence Gene ◽

Type I ◽

Type Ii ◽

Methicillin Resistant ◽

Type Iv ◽

Type V

Abstract Objective: This study was designed to discover the dissemination of virulence genes in Methicillin-resistant Staphylococcus aureus from clinical and environmental settings. Results: The virulence gene such as sea (n=54), seb (n=21), eta (n=27), etb (n=2), cna (n=24), ica (n=2) and tst (n=30) was revealed from this study. Different SCCmec types such as type I, type II, type III, type IV, type V, type VI, type VII, type VIII and type XII were detected among sixty three MRSA isolates where SCCmec type II having ST1551 and type V with ST2416 were found to be associated with multidrug resistance and were highly prevalent in the study area.

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