Characterization of a new multidrug-resistant Brazilian K. pneumoniae isolate and 172 Klebsiella spp. sequenced strains: Genomic island, multilocus sequence typing and capsule locus dataset

ABSTRACT Strains of multidrug-resistant Salmonella enterica serovar Typhimurium DT104 (DT104) and S. enterica serovar Agona (Agona) have been found to harbor Salmonella genomic island 1 (SGI1), a 43-kb genomic region that contains many of the drug resistance genes. Such strains are resistant to ampicillin (pse-1), chloramphenicol/florfenicol (floR), streptomycin/spectinomycin (aadA2), sulfonamides (sul1), and tetracycline [tet(G)] (commonly called the ACSSuT phenotype). All five resistance genes are found in a 13-kb multidrug resistance (MDR) region consisting of an unusual class I integron structure related to In4. We examined DT104 and Agona strains that exhibited other resistance phenotypes to determine if the resistance genes were associated with variant SGI1 MDR regions. All strains were found to harbor variant SGI1-like elements by using a combination of Southern hybridization, PCR mapping, and sequencing. Variant SGI1-like elements were found with MDR regions consisting of (i) an integron consisting of the SGI1 MDR region with the addition of a region containing a putative transposase gene (orf513) and dfrA10 located between duplicated qacEΔ1/sulI genes (SGI1-A; ACSSuTTm); (ii) an integron with either an aadA2 (SSu) or a pse-1 (ASu) cassette (SGI1-C and SGI1-B, respectively); (iii) an integron consisting of the SGI1-C MDR region plus an orf513/dfrA10 region as in SGI1-A (SGI1-D; ASSuTm; ampicillin resistance due to a TEM β-lactamase); and (iv) an integron related to that in SGI1 but which contains a 10-kb inversion between two copies of IS6100, one which is inserted in floR (SGI1-E; ASSuT). We hypothesize that the MDR of SGI1 is subject to recombinational events that lead to the various resistance phenotypes in the Salmonella strains in which it is found.

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Identification of Three Major Clones of Multiply Antibiotic-Resistant Streptococcus pneumoniae in Taiwanese Hospitals by Multilocus Sequence Typing

Journal of Clinical Microbiology ◽

10.1128/jcm.36.12.3514-3519.1998 ◽

1998 ◽

Vol 36 (12) ◽

pp. 3514-3519 ◽

Cited By ~ 70

Author(s):

Zhi-Yuan Shi ◽

Mark C. Enright ◽

Paul Wilkinson ◽

David Griffiths ◽

Brian G. Spratt

Keyword(s):

Streptococcus Pneumoniae ◽

Molecular Typing ◽

Multilocus Sequence Typing ◽

Housekeeping Genes ◽

Multidrug Resistant ◽

Single Locus ◽

Allelic Profile ◽

Antibiotic Resistant ◽

Typing Procedure

In this paper we demonstrate the advantages of a new molecular typing procedure, multilocus sequence typing, for the unambiguous characterization of penicillin-resistant pneumococci. The sequences of ∼450-bp fragments of seven housekeeping genes were determined for 74 penicillin-resistant Taiwanese isolates of Streptococcus pneumoniae (MIC of penicillin > 0.5 μg/ml). The combination of alleles at the seven loci defined an allelic profile for each strain, and a dendrogram, based on the pairwise mismatches in allelic profiles, grouped 86% of the isolates into one of three penicillin-resistant clones for which the MICs of penicillin were 1 to 2 μg/ml. Isolates within each clone had identical alleles at all seven loci or differed at only a single locus, and the fingerprints of their pbp1A, pbp2B, and pbp2X genes were uniform. Isolates of the Taiwan-19F clone and the Taiwan-23F clone were resistant to penicillin, tetracycline, and erythromycin but were susceptible to chloramphenicol. A second serotype 23F clone and serotype 19F variants of this clone were resistant to penicillin, tetracycline, chloramphenicol, and, in some cases, erythromycin. Comparisons of the allelic profiles of the three major clones with those of reference isolates of the known penicillin-resistant clones showed that the Taiwan-19F and Taiwan-23F clones were previously undescribed, whereas the second serotype 23F clone was indistinguishable from the Spanish multidrug-resistant serotype 23F clone. Single isolates of the Spanish penicillin-resistant serotype 9V clone and the Spanish multidrug-resistant serotype 6B clone were also identified in the collection.

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Isolation and characterization of multidrug-resistant Klebsiella spp. isolated from shrimp imported from Thailand

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2012.02.002 ◽

2012 ◽

Vol 155 (3) ◽

pp. 179-184 ◽

Cited By ~ 25

Author(s):

Mohamed Nawaz ◽

S.A. Khan ◽

Q. Tran ◽

K. Sung ◽

A.A. Khan ◽

...

Keyword(s):

Multidrug Resistant ◽

Isolation And Characterization ◽

Klebsiella Spp

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Characterization of Bacteria and Inducible Phages in an Intensive Care Unit

Journal of Clinical Medicine ◽

10.3390/jcm8091433 ◽

2019 ◽

Vol 8 (9) ◽

pp. 1433 ◽

Cited By ~ 2

Author(s):

Pacífico ◽

Hilbert ◽

Sofka ◽

Dinhopl ◽

Pap ◽

...

Keyword(s):

Escherichia Coli ◽

Intensive Care ◽

Environmental Samples ◽

Multidrug Resistant ◽

Antimicrobial Treatment ◽

Bacterial Isolates ◽

Surface Samples ◽

Klebsiella Spp ◽

Synergistic Relationship

Intensive care units (ICUs) are critical locations for the transmission of pathogenic and opportunistic microorganisms. Bacteria may develop a synergistic relationship with bacteriophages and more effectively resist various stresses, enabling them to persist despite disinfection and antimicrobial treatment. We collected 77 environmental samples from the surroundings of 12 patients with infection/colonizations by Escherichia coli, Staphylococcus aureus or Klebsiella spp in an ICU in Austria. Surface swabs were tested for lytic phages and bacterial isolates for mitomycin C-inducible prophages. No lytic bacteriophages were detected, but S. aureus was isolated from the surroundings of all patients. About 85% of the colonies isolated from surface samples were resistant to antimicrobials, with 94% of them multidrug resistant. Two inducible temperate bacteriophages—myovirus vB_EcoM_P5 and siphovirus vB_SauS_P9—were recovered from two clinical isolates. Staphylococci phage vB_SauS_P9 lysed S. aureus isolates from the surface swabs collected from the surroundings of three patients. No transductants were obtained on propagation in phage-sensitive antimicrobial-resistant isolates. The two phages were sensitive to 0.25% (v/v) of the disinfectant TPH Protect, which eliminated viable phages after 15 min. Coliphage vB_EcoM_P5 was inactivated at 70 °C and staphylococci phage vB_SauS_P9 at 60 °C after 60 min.

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Risk Factors of Multidrug-resistant Klebsiella spp. in Hospitalized Cancer Patients in Erbil Governate, Iraq

Journal of Research on the Lepidoptera ◽

10.36872/lepi/v51i3/301174 ◽

2020 ◽

Vol 51 (3) ◽

pp. 123-140

Author(s):

FATTMA A. ALI ◽

SUSAN F. KHADHEM AL-SUDANI ◽

GAILAN CHWAIS HASSAN ◽

SEVAN HASSAN BAKIR

Keyword(s):

Risk Factors ◽

Cancer Patients ◽

Multidrug Resistant ◽

Klebsiella Spp

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Distribution of Pathogens Involved in Infections in Oncological Patients and their Antibiotic Resistance Profile

Revista de Chimie ◽

10.37358/rc.19.12.7747 ◽

2020 ◽

Vol 70 (12) ◽

pp. 4287-4294

Keyword(s):

Antibiotic Resistance ◽

Cancer Patients ◽

Bacterial Pathogen ◽

Multidrug Resistant ◽

Diffusion Method ◽

Resistance Pattern ◽

Antibiotic Resistance Profile ◽

Oncological Patients ◽

Eastern European Countries ◽

Klebsiella Spp

Cancer is the second leading cause of death in Romania and worldwide. Cancer patients are at increasing risk of acquiring bacterial infection with multi-resistant germs, including multidrug-resistant (MDR) strains of Gram-negative bacteria involved in nosocomial infection. Romania is one of the South-Eastern European countries with one of the highest prevalence rates of MDR pathogens. To determine the resistance pattern of bacterial profile and antibiotic resistance pattern in cancer patients admitted at the County Emergency Clinical Hospital Craiova, Romania. A retrospective study of bacterial pathogens was carried out on 90 adult cancer patients admitted from January to December 2018. The analysis of the resistance patterns for the action of the appropriate antibiotics was performed using Vitek 2 Compact system and diffusion method. In this study there were analysed 92 samples from 90 oncological patients (37-86 years). A total of 157 bacterial isolates were obtained, of which 37 strains of Staphylococcus aureus (23.56%), followed by Streptococcus pneumoniae (23- 14.64%), Klebsiella spp. and Escherichia coli (22 - 14,01%). The most common isolates were from respiratory tract (86 isolates - 54.77%). High rates of MDR were found for E. coli (63.63%), MRSA (61,11%) and Klebsiella spp. (54,54%), while one third of the isolated strains of Pseudomonas aeruginosa, Acinetobacter spp. and Proteus spp. were MDR. The findings of this study may be the basis for further more extensive studies highlighting the germs involved in the infectious pathology of cancer patients, in order to determine the antimicrobial resistance and to improve the methods of prophylaxis and treatment. Keywords: multidrug resistance (MDR), cancer patients, bacterial pathogen

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Genomic characterization of multidrug-resistant Bacillus toyonensis strain 4HC1 isolated from marine plastic in Norway

Journal of Global Antimicrobial Resistance ◽

10.1016/j.jgar.2021.07.002 ◽

2021 ◽

Author(s):

Vera Radisic ◽

Nachiket P. Marathe

Keyword(s):

Multidrug Resistant ◽

Genomic Characterization ◽

Bacillus Toyonensis

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Klebsiella spp. cause severe and fatal disease in Mozambican children: antimicrobial resistance profile and molecular characterization

BMC Infectious Diseases ◽

10.1186/s12879-021-06245-x ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Arsénia J. Massinga ◽

Marcelino Garrine ◽

Augusto Messa ◽

Nélio A. Nobela ◽

Nadia Boisen ◽

...

Keyword(s):

Antimicrobial Susceptibility ◽

Case Fatality ◽

Multidrug Resistant ◽

Childhood Mortality ◽

Fatal Disease ◽

Appropriate Treatment ◽

Antimicrobial Resistance Profile ◽

Postmortem Blood ◽

Postmortem Studies ◽

Klebsiella Spp

Abstract Background Klebsiella spp. are important pathogens associated with bacteremia among admitted children and is among the leading cause of death in children < 5 years in postmortem studies, supporting a larger role than previously considered in childhood mortality. Herein, we compared the antimicrobial susceptibility, mechanisms of resistance, and the virulence profile of Klebsiella spp. from admitted and postmortem children. Methods Antimicrobial susceptibility and virulence factors of Klebsiella spp. recovered from blood samples collected upon admission to the hospital (n = 88) and postmortem blood (n = 23) from children < 5 years were assessed by disk diffusion and multiplex PCR. Results Klebsiella isolates from postmortem blood were likely to be ceftriaxone resistant (69.6%, 16/23 vs. 48.9%, 43/88, p = 0.045) or extended-spectrum β-lactamase (ESBL) producers (60.9%, 14/23 vs. 25%, 22/88, p = 0.001) compared to those from admitted children. blaCTX-M-15 was the most frequent ESBL gene: 65.3%, 9/14 in postmortem isolates and 22.7% (5/22) from admitted children. We found higher frequency of genes associated with hypermucoviscosity phenotype and invasin in postmortem isolates than those from admitted children: rmpA (30.4%; 7/23 vs. 9.1%, 8/88, p = 0.011), wzi-K1 (34.7%; 8/23 vs. 8%; 7/88, p = 0.002) and traT (60.8%; 14/23 vs. 10.2%; 9/88, p < 0.0001), respectively. Additionally, serine protease auto-transporters of Enterobacteriaceae were detected from 1.8% (pic) to 12.6% (pet) among all isolates. Klebsiella case fatality rate was 30.7% (23/75). Conclusion Multidrug resistant Klebsiella spp. harboring genes associated with hypermucoviscosity phenotype has emerged in Mozambique causing invasive fatal disease in children; highlighting the urgent need for prompt diagnosis, appropriate treatment and effective preventive measures for infection control.

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