scholarly journals Endosulfan promotes cell proliferation and extracellular matrix accumulation through TGF-β/Smad signaling pathway in HRMCs

2021 ◽  
Vol 228 ◽  
pp. 113040
Author(s):  
Shiwen Liu ◽  
Yumeng Hu ◽  
Yue Wang ◽  
Yeqing Sun ◽  
Shu-Lan Qin ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Proliferation ◽  
Signaling Pathway ◽  
Smad Signaling ◽  
Smad Signaling Pathway ◽  
Matrix Accumulation

scholarly journals MiRNA-21 Regulates Bronchial Epithelial Cell Proliferation by Activating Tgfβ1/Smad Signaling Pathway and Its Correlation with Asthma Severity in Children

2021 ◽  
Author(s):  
Yang Kang ◽  
Minghui Bai ◽  
Liling Deng ◽  
Linbo Fan ◽  
Xing Wang
Keyword(s):  
Cell Proliferation ◽  
Signaling Pathway ◽  
Bronchial Epithelial Cell ◽  
Control Group ◽  
Healthy Children ◽  
Bronchial Epithelial ◽  
Smad Signaling ◽  
Smad Signaling Pathway ◽  
Tgf Β1

Background: This research was designed to probe into the role of miRNA-21 in the pathogenesis of childhood asthma and its correlation with the severity. Methods: Fifty-four children with bronchial asthma admitted to the Third Affiliated Hospital of Qiqihar Medical University from Jun 2018 to Dec 2019 were included. Forty nine healthy children underwent physical examination at this time period were also enrolled. The miR-21 expression in peripheral blood serum was analyzed by qRT-PCR. The relationship between the expression and severity of asthma in children was explored by Spearman correlation analysis and ROC curve. Bronchial epithelial cell lines were cultured in vitro and divided into blank control group, negative control group and miR-21 inhibition and activation group. The changes of cell proliferation after treatment were detected by CCK-8 test in different groups. The expression of TGF-β1/Smad signaling pathway protein in cells was assessed by Western blot (WB). Results: Compared with that of healthy children, the miR-21 expression in peripheral blood serum of asthmatic children was higher (P<0.001). MiR-21 expression was positively correlated with the severity of illness (r=0.853, P<0.001). The results of cell experiments in vitro signified that miR-21 can promote the proliferation of bronchial epithelial cells, and may be involved in regulating the expression of TGF-β1/ Smad3 signaling pathway, thus affecting cell proliferation. Conclusion: miRNA-21 regulates the proliferation of bronchial epithelial cells by activating TGFβ1/Smad signaling pathway. And it is positively correlated with the severity of asthma in children.

scholarly journals Let-7a suppresses cell proliferation via the TGF-β/SMAD signaling pathway in cervical cancer

2016 ◽  
Vol 36 (6) ◽  
pp. 3275-3282 ◽  
Author(s):  
Tianhui Wu ◽  
Xin Chen ◽  
Rui Peng ◽  
Handeng Liu ◽  
Pin Yin ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Proliferation ◽  
Signaling Pathway ◽  
Smad Signaling ◽  
Smad Signaling Pathway

scholarly journals Effect of Kangxianling Decoction on Expression of TGF-β1/Smads and Extracellular Matrix Deposition

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Jing Ji ◽  
Liqun He
Keyword(s):  
Extracellular Matrix ◽  
Growth Factor ◽  
Signaling Pathway ◽  
Renal Fibrosis ◽  
Pathophysiological Mechanism ◽  
Smad Signaling ◽  
Matrix Deposition ◽  
Collagen Iii ◽  
Smad Signaling Pathway ◽  
Group 5

Kangxianling (KXL) decoction is a traditional Chinese herbal formulation which has been used to treat early and midterm chronic renal failure. Renal fibrosis is a common characteristic of progressive chronic kidney diseases (CKD). The formation of renal fibrosis is caused by kidney trauma, infection, and immune response. The pathophysiological mechanism of renal fibrosis was mainly due to increased collagen synthesis in the kidney, decreased degradation, and a large amount of extracellular matrix (ECM) deposition. The purpose of this study was intended to evaluate the effect of Kangxianling decoction on expression of TGF-β1/Smad signaling pathway in renal fibrosis rats. 50 specific pathogen-free Sprague Dawley (SPF SD) rats were randomly divided into five groups: control group, sham group, 5/6 nephrectomy model group, 5/6 nephrectomy model plus KXL decoction (21g /kg) group, and 5/6 nephrectomy model plus Losartan Potassium (LP) (33.3 g/kg) group. The rats were all sacrificed after two months and the left kidney tissue was sampled. HE staining was used to observe the renal pathological changes and the score of kidney damage was made. Masson staining was used to observe the degree of renal fibrosis. Immunohistochemical staining, western blot, and qRT-PCR were used to detect the expression levels of related molecules in TGF-β1/Smad signaling pathway. The results suggested that KXL could lighten renal histopathology damage, downregulate the expression of TGF-β1 (transforming growth factor-β1), Smad2/3, CTGF (connective tissue growth factor), Collagen I, and Collagen III, and upregulate the expression level of Smad7.

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