Low dose of cholecalciferol induces antidepressant-like effect in the middle aged ovariectomized female rats treated with 17β-estradiol

2019 ◽  
Vol 29 ◽  
pp. S85-S86
Author(s):  
J. Fedotova ◽  
S. Pivina
Keyword(s):  
Low Dose ◽  
Female Rats ◽  
Middle Aged ◽  
17Β Estradiol

scholarly journals Glyphosate-based herbicide enhances the uterine sensitivity to estradiol in rats

2018 ◽  
Vol 239 (2) ◽  
pp. 197-213 ◽  
Author(s):  
Marlise Guerrero Schimpf ◽  
María M Milesi ◽  
Enrique H Luque ◽  
Jorgelina Varayoud
Keyword(s):  
Cell Proliferation ◽  
Endometrial Hyperplasia ◽  
Low Dose ◽  
Saline Solution ◽  
Female Rats ◽  
Postnatal Exposure ◽  
Rat Uterus ◽  
Mrna Extraction ◽  
17Β Estradiol ◽  
Prepubertal Rats

In a previous work, we detected that postnatal exposure to a glyphosate-based herbicide (GBH) alters uterine development in prepubertal rats causing endometrial hyperplasia and increasing cell proliferation. Our goal was to determine whether exposure to low dose of a GBH during postnatal development might enhance the sensitivity of the uterus to an estrogenic treatment. Female Wistar pups were subcutaneously injected with saline solution (control) or GBH using the reference dose (2 mg/kg/day, EPA) on postnatal days (PND) 1, 3, 5 and 7. At weaning (PND21), female rats were bilaterally ovariectomized and treated with silastic capsules containing 17β-estradiol (E2, 1 mg/mL) until they were 2 months of age. On PND60, uterine samples were removed and processed for histology, immunohistochemistry and mRNA extraction to evaluate: (i) uterine morphology, (ii) uterine cell proliferation by the detection of Ki67, (iii) the expression of the estrogen receptors alpha (ESR1) and beta (ESR2) and (iv) the expression of WNT7A and CTNNB1. GBH-exposed animals showed increased luminal epithelial height and stromal nuclei density. The luminal and glandular epithelium were markedly hyperplastic in 43% of GBH-exposed animals. GBH exposure caused an increase in E2-induced cell proliferation in association with an induction of both ESR1 and ESR2. GBH treatment decreased membranous and cytoplasmic expression of CTNNB1 in luminal and glandular epithelial cells and increased WNT7A expression in the luminal epithelium. These results suggest that early postnatal exposure to a GBH enhances the sensitivity of the rat uterus to estradiol and induces histomorphological and molecular changes associated with uterine hyperplasia.

scholarly journals A long-term cyclic plus tonic regimen of 17β-estradiol improves the ability to handle a high spatial working memory load in ovariectomized middle-aged female rats

2020 ◽  
Vol 118 ◽  
pp. 104656 ◽  
Author(s):  
Stephanie V. Koebele ◽  
Kenji J. Nishimura ◽  
Heather A. Bimonte-Nelson ◽  
Salma Kemmou ◽  
J. Bryce Ortiz ◽  
...  
Keyword(s):  
Working Memory ◽  
Spatial Working Memory ◽  
Memory Load ◽  
Female Rats ◽  
Middle Aged ◽  
Working Memory Load ◽  
17Β Estradiol

Abstract WMP42: Astrocyte Specific rAAV-mediated Insulin-Like Growth Factor-1 Expression in Aging Female Rats Increases Post-Stroke Survival and Sensory Motor Performance

Stroke ◽  
2016 ◽  
Vol 47 (suppl_1) ◽  
Author(s):  
Andre K Okoreeh ◽  
Shameena Bake ◽  
Farida Sohrabji
Keyword(s):  
Motor Performance ◽  
Low Dose ◽  
Female Rats ◽  
Specific Gene ◽  
High Dose ◽  
Middle Aged ◽  
Post Stroke ◽  
Post Surgery ◽  
Virus Serotype ◽  
Sensory Motor

Background and Purpose: Our previous work shows that middle aged female rats sustain larger strokes as compared to younger female rats. With age, circulating and brain parenchymal levels of IGF-1 are reduced. Exogenous IGF-1 treatment improves infarct volume in aging females. Our recent studies show that astrocytes from aging females synthesize less IGF-1. Here we tested the hypothesis that elevation of astrocyte derived IGF-1 would improve stroke impairment in older female rats. Methods: Middle-aged (10-12 month old; acyclic) female rats were injected with adeno-associated virus serotype 5 (rAAV5) into the cortex and striatum. rAAV5 was packaged with the coding sequence of the IGF-1 gene downstream of an astrocyte-specific gene (GFAP). The construct contained the mCherry reporter gene. Control rAAV consisted of an identical shuttle vector construct without the IGF-1 gene. In separate experiments, two titers of virus were injected: high dose (5 X 10 12 VP/mL) or low dose (5 X 10 11 VP/mL). Three to four weeks after injection, middle-cerebral artery occlusion via an intraluminal suture for ninety minutes was performed followed by reperfusion. Post-surgery survival was monitored as well as sensory motor function using the vibrissae evoked forelimb placement task. Results: Specificity of IGF-1 expression was confirmed by visualization of the mCherry reporter under fluorescent illumination and immunohistochemistry. Post stroke survival was improved in animals that received the high dose rAAV-IGF-1 animals a 5-day period (p<0.001). Low dose rAAV-IGF-1 did not affect post stroke survival, however sensory motor performance was preserved in this group. In low dose control animals, ischemic stroke impaired performance on the vibrissae evoked forelimb placement task. Impairment was seen in the same-side and cross-midline task performance on the limb contralateral to the infarct and cross midline task on the limb ipsilateral to the infarcted side (p<0.05). No significant deficits were seen in the rAAV-IGF-1 low dose treated animals. Conclusion: These data support the hypothesis that increasing astrocytic IGF-1 in aging females improves post stroke survival and behavior outcomes.

Soybean phytoestrogens reduce 27-hydroxycholesterol concentration in the liver of middle-aged female rats

2017 ◽  
Author(s):  
Branka Šošić-Jurjević ◽  
Snežana Janković ◽  
Dieter Lutjohann ◽  
Ivana Jarić ◽  
Marko Miler ◽  
...  
Keyword(s):  
Female Rats ◽  
Middle Aged

Effect of Fetal and Neonatal Hypothyroidism on Glucose Tolerance in Middle-Aged Female Rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Sajad Jeddi ◽  
Saeedeh Khalifi ◽  
Mahboubeh Ghanbari ◽  
Asghar Ghasemi
Keyword(s):  
Glucose Tolerance ◽  
Plasma Glucose ◽  
Female Offspring ◽  
Female Rats ◽  
Control Group ◽  
Female Rat ◽  
Middle Aged ◽  
Intravenous Glucose ◽  
Neonatal Hypothyroidism ◽  
Glucose Levels

Background and objective: The effects of hypothyroidism during pregnancy and lactation on carbohydrate metabolism have been mostly studied in male animals. The aim of this study is therefore to investigate effect of fetal and neonatal hypothyroidism (FH and NH) on the glucose tolerance in middle-aged female rat offspring. Methods: Pregnant female rats were divided into three groups: Rats in the control group consumed tap water, while those in the FH and NH groups consumed 250 mg/L of 6-propyl-2-thiouracil (PTU) in their drinking water during gestation or lactation periods, respectively. After weaning, the female offspring were separated and divided into 3 groups (n=8/group): Control, FH, and NH. Body weight was recorded monthly and intravenous glucose tolerance test (IVGTT) was performed at month 12. Results: Compared to controls, female rats in the FH group had significantly higher plasma glucose levels than controls throughout the IVGTT except at min 60. Values at min 5 of the FH and control group were 196.1±1.9 and 155.3±5.9 mg/dL, respectively (P<0.05). In the NH group, plasma glucose levels were significantly higher only at min 5 (185.7±14.1 vs. 155.3±5.9 mg/dL, P<0.05). Conclusion: Hypothyroidism during fetal or neonatal periods caused glucose intolerance in middle-aged female offspring rats.

scholarly journals Vulnerability factors for mephedrone-induced conditioned place preference in rats—the impact of sex differences, social-conditioning and stress

2021 ◽  
Author(s):  
Olga Wronikowska ◽  
Maria Zykubek ◽  
Łukasz Kurach ◽  
Agnieszka Michalak ◽  
Anna Boguszewska-Czubara ◽  
...  
Keyword(s):  
Sex Differences ◽  
Conditioned Place Preference ◽  
Low Dose ◽  
Social Factor ◽  
Place Preference ◽  
Female Rats ◽  
Male Rats ◽  
Social Conditioning ◽  
Male And Female Rats ◽  
The Impact

Abstract Rationale Mephedrone is a frequently overused drug of abuse that belongs to the group of novel psychoactive substances. Although its mechanism of action, as well as toxic and psychoactive effects, has been widely studied, the role of different factors that could contribute to the increased vulnerability to mephedrone abuse is still poorly understood. Objectives The aim of the presented study was to assess the impact of several factors (sex differences, social-conditioning, and chronic mild unpredictable stress — CMUS) on the liability to mephedrone-induced reward in Wistar rats. Methods The rewarding effects of mephedrone in male and female rats were assessed using the conditioned place preference (CPP) procedure. Furthermore, the impact of social factor and stress was evaluated in male rats using social-CPP and CMUS-dependent CPP, respectively. Results Mephedrone induced classic-CPP in female (10 mg/kg), as well as in male (10 and 20 mg/kg) rats. However, the impact of mephedrone treatment during social-CPP was highly dose-dependent as the rewarding effects of low dose of mephedrone (5 mg/kg; non-active in classic-CPP) were potentiated when administered during social-conditioning. Interestingly, social-conditioning with a higher dose of 20 mg/kg (that induced classic-CPP) was able to reverse these effects. Finally, CMUS potentiated rewarding effects of a low dose of mephedrone (5 mg/kg) and increased the level of corticosterone in rats’ prefrontal cortex and hippocampus. Conclusions Altogether, the presented results give new insight into possible factors underlying the vulnerability to mephedrone abuse and can serve as a basis for further studies assessing mechanisms underlying observed effects.

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