scholarly journals Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities

2020 ◽  
Vol 124 ◽  
pp. 307-319 ◽  
Author(s):  
Mikel Añibarro-Ortega ◽  
José Pinela ◽  
Ana Ćirić ◽  
Valter Martins ◽  
Filomena Rocha ◽  
...  
Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 703
Author(s):  
Severino Zara ◽  
Giacomo L. Petretto ◽  
Alberto Mannu ◽  
Giacomo Zara ◽  
Marilena Budroni ◽  
...  

The production of saffron spice generates large quantities of plant by-products: over 90% of the plant material collected is discarded, and a consideration fraction of this waste is plant stamens. This work investigated the chemical composition and the antimicrobial activities of the non-polar fraction extracted from four different saffron flower stamens. The chemical composition of ethereal extracts of the saffron stamens was qualitatively assessed by means of gas–chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) analyses. These analyses revealed ethereal extracts to possess a high polyunsaturated fatty acid content. In vitro antibacterial activity of stamen extracts showed no large differences between Gram-positive and Gram-negative bacteria in terms of minimal inhibitory concentration (MIC). In food matrix microbial analysis of the bacterial strains belonging to the main foodborne pathogen species, including Staphylococcus aureus DSM 20231, Escherichia coli DSM 30083, and Listeria monocytogenes DSM 20600, using low-fat UHT milk, revealed a statistically significant reduction in the number of cells (particularly for E. coli and S. aureus with a complete elimination of the population of the two target bacteria following incubation in diethyl ether extracts of saffron stamen (DES) at high concentrations tested, both at 37 °C and 6 °C (for 48 h and 7 days, respectively). A synergic effect was observed when the pathogens were incubated at 6 °C with DES. This work shows these by-products to be excellent sources of bioactive compounds, which could be exploited in high-added-value products, such as food, cosmetics, and drugs.


PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5991 ◽  
Author(s):  
Carla de Fatima Alves Nonato ◽  
Débora Odília Duarte Leite ◽  
Rafael Caldas Pereira ◽  
Aline Augusti Boligon ◽  
Jaime Ribeiro-Filho ◽  
...  

BackgroundFruit consumption is currently considered beyond the nutritional aspects because of the important roles in disease prevention and benefits to health. The objective of this study was to characterize the chemical profile and evaluate the antioxidant and antimicrobial properties of different fractions obtained from fruit pulps ofMauritia flexuosa(MFFs).MethodsInitially, chloroform, ethyl acetate and ethanol fractions were obtained from the pulps. Quantifications of total phenols and flavonoids were performed using the methods of Folin-Ciocalteu and complexation with aluminum chloride, respectively. Standard samples were used to identify and quantify phenolic acids and flavonoids using high-performance liquid chromatography with a diode-array detector (HPLC-DAD). The antioxidant capacity of the fractions was verified by sequestration of the free radical 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and iron chelating activity. The antimicrobial activity was determined using the microdilution method and the modulating activity was determined using sub-inhibitory concentrations of the fractions in association with antibiotics.ResultsThe chemical analyzes revealed the presence of catechin, caffeic acid, rutin, orientin, quercetin, apigenin, luteolin and kaempferol, where all are present in the ethyl acetate fraction. The fractions exhibited moderate antioxidant and antimicrobial activities against Gram-positive andCandidastrains in addition to modulating the activity of conventional antibiotics. The most expressive result was obtained from the association of the chloroform fraction with cefotaxime, which produced a synergistic effect, reducing the minimum inhibitory concentration (MIC) of the antibiotic from 1,024 to 256 μg/mL.DiscussionThe fractions presented a constitution rich in phenolic compounds, especially flavonoids. The data obtained demonstrated that the fractions presented moderate antioxidant activity by acting both as primary and secondary antioxidants. The fractions presented antimicrobial and antibiotic potentiating activities, being the first record of modulating effect of fractions of this species against the studied microbial strains, but failed in modulating the activity of antifungal drugs, indicating that this plant has the potential to be used in the development of therapeutic alternatives against resistant bacteria. The constitution phenolic the fractions may be responsible for their pharmacological properties in vitro.


2008 ◽  
Vol 11 (4) ◽  
pp. 727-737 ◽  
Author(s):  
Rosa Ana Pérez Martín ◽  
Lucía Vela Hortigüela ◽  
Paloma Lorenzo Lozano ◽  
Ma Dolores Rojo Cortina ◽  
Cristina de Lorenzo Carretero

2017 ◽  
Vol 55 (1) ◽  
pp. 1155-1161 ◽  
Author(s):  
Venkanna Banothu ◽  
Chandrasekharnath Neelagiri ◽  
Uma Adepally ◽  
Jayalakshmi Lingam ◽  
Kesavaharshini Bommareddy

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Yu-Chang Su ◽  
Kuan-Ping Hsu ◽  
Chen-Lung Ho

The chemical composition and in vitro anti-inflammatory, antioxidant and antimicrobial activities of the leaf essential oil of Machilus konishii has been investigated. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC–FID and GC–MS. Sixty-six compounds were identified, representing 100% of the oil. The main components identified were α-pinene (33.9%), β-pinene (13.9%), and thymol (12.0%). The leaf oil was able to reduce nitric oxide production by lipopolysaccharide-activated murine macrophages RAW 264.7 without reducing the cell viability. In addition, the leaf oil showed strong antioxidant and antimicrobial activities. The major ingredient of the oil that was responsible for the anti-inflammatory, antioxidant and antimicrobial activities was thymol.


Sign in / Sign up

Export Citation Format

Share Document